Job ID = 1293997 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 64,882,446 reads read : 64,882,446 reads written : 64,882,446 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:46 64882446 reads; of these: 64882446 (100.00%) were unpaired; of these: 6308918 (9.72%) aligned 0 times 40580989 (62.55%) aligned exactly 1 time 17992539 (27.73%) aligned >1 times 90.28% overall alignment rate Time searching: 00:31:46 Overall time: 00:31:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 16917213 / 58573528 = 0.2888 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:07:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:07:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:07:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:07:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:07:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:07:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:07:58: 1000000 INFO @ Mon, 03 Jun 2019 05:07:58: 1000000 INFO @ Mon, 03 Jun 2019 05:07:58: 1000000 INFO @ Mon, 03 Jun 2019 05:08:10: 2000000 INFO @ Mon, 03 Jun 2019 05:08:10: 2000000 INFO @ Mon, 03 Jun 2019 05:08:10: 2000000 INFO @ Mon, 03 Jun 2019 05:08:22: 3000000 INFO @ Mon, 03 Jun 2019 05:08:22: 3000000 INFO @ Mon, 03 Jun 2019 05:08:22: 3000000 INFO @ Mon, 03 Jun 2019 05:08:33: 4000000 INFO @ Mon, 03 Jun 2019 05:08:33: 4000000 INFO @ Mon, 03 Jun 2019 05:08:33: 4000000 INFO @ Mon, 03 Jun 2019 05:08:44: 5000000 INFO @ Mon, 03 Jun 2019 05:08:45: 5000000 INFO @ Mon, 03 Jun 2019 05:08:45: 5000000 INFO @ Mon, 03 Jun 2019 05:08:56: 6000000 INFO @ Mon, 03 Jun 2019 05:08:56: 6000000 INFO @ Mon, 03 Jun 2019 05:08:56: 6000000 INFO @ Mon, 03 Jun 2019 05:09:07: 7000000 INFO @ Mon, 03 Jun 2019 05:09:07: 7000000 INFO @ Mon, 03 Jun 2019 05:09:07: 7000000 INFO @ Mon, 03 Jun 2019 05:09:19: 8000000 INFO @ Mon, 03 Jun 2019 05:09:19: 8000000 INFO @ Mon, 03 Jun 2019 05:09:19: 8000000 INFO @ Mon, 03 Jun 2019 05:09:30: 9000000 INFO @ Mon, 03 Jun 2019 05:09:30: 9000000 INFO @ Mon, 03 Jun 2019 05:09:30: 9000000 INFO @ Mon, 03 Jun 2019 05:09:41: 10000000 INFO @ Mon, 03 Jun 2019 05:09:41: 10000000 INFO @ Mon, 03 Jun 2019 05:09:41: 10000000 INFO @ Mon, 03 Jun 2019 05:09:53: 11000000 INFO @ Mon, 03 Jun 2019 05:09:53: 11000000 INFO @ Mon, 03 Jun 2019 05:09:53: 11000000 INFO @ Mon, 03 Jun 2019 05:10:04: 12000000 INFO @ Mon, 03 Jun 2019 05:10:04: 12000000 INFO @ Mon, 03 Jun 2019 05:10:04: 12000000 INFO @ Mon, 03 Jun 2019 05:10:15: 13000000 INFO @ Mon, 03 Jun 2019 05:10:15: 13000000 INFO @ Mon, 03 Jun 2019 05:10:16: 13000000 INFO @ Mon, 03 Jun 2019 05:10:27: 14000000 INFO @ Mon, 03 Jun 2019 05:10:27: 14000000 INFO @ Mon, 03 Jun 2019 05:10:27: 14000000 INFO @ Mon, 03 Jun 2019 05:10:38: 15000000 INFO @ Mon, 03 Jun 2019 05:10:38: 15000000 INFO @ Mon, 03 Jun 2019 05:10:38: 15000000 INFO @ Mon, 03 Jun 2019 05:10:50: 16000000 INFO @ Mon, 03 Jun 2019 05:10:50: 16000000 INFO @ Mon, 03 Jun 2019 05:10:50: 16000000 INFO @ Mon, 03 Jun 2019 05:11:01: 17000000 INFO @ Mon, 03 Jun 2019 05:11:01: 17000000 INFO @ Mon, 03 Jun 2019 05:11:01: 17000000 INFO @ Mon, 03 Jun 2019 05:11:12: 18000000 INFO @ Mon, 03 Jun 2019 05:11:12: 18000000 INFO @ Mon, 03 Jun 2019 05:11:12: 18000000 INFO @ Mon, 03 Jun 2019 05:11:24: 19000000 INFO @ Mon, 03 Jun 2019 05:11:24: 19000000 INFO @ Mon, 03 Jun 2019 05:11:24: 19000000 INFO @ Mon, 03 Jun 2019 05:11:35: 20000000 INFO @ Mon, 03 Jun 2019 05:11:35: 20000000 INFO @ Mon, 03 Jun 2019 05:11:35: 20000000 INFO @ Mon, 03 Jun 2019 05:11:46: 21000000 INFO @ Mon, 03 Jun 2019 05:11:46: 21000000 INFO @ Mon, 03 Jun 2019 05:11:47: 21000000 INFO @ Mon, 03 Jun 2019 05:11:58: 22000000 INFO @ Mon, 03 Jun 2019 05:11:58: 22000000 INFO @ Mon, 03 Jun 2019 05:11:58: 22000000 INFO @ Mon, 03 Jun 2019 05:12:09: 23000000 INFO @ Mon, 03 Jun 2019 05:12:09: 23000000 INFO @ Mon, 03 Jun 2019 05:12:09: 23000000 INFO @ Mon, 03 Jun 2019 05:12:20: 24000000 INFO @ Mon, 03 Jun 2019 05:12:20: 24000000 INFO @ Mon, 03 Jun 2019 05:12:20: 24000000 INFO @ Mon, 03 Jun 2019 05:12:31: 25000000 INFO @ Mon, 03 Jun 2019 05:12:31: 25000000 INFO @ Mon, 03 Jun 2019 05:12:31: 25000000 INFO @ Mon, 03 Jun 2019 05:12:43: 26000000 INFO @ Mon, 03 Jun 2019 05:12:43: 26000000 INFO @ Mon, 03 Jun 2019 05:12:43: 26000000 INFO @ Mon, 03 Jun 2019 05:12:54: 27000000 INFO @ Mon, 03 Jun 2019 05:12:54: 27000000 INFO @ Mon, 03 Jun 2019 05:12:54: 27000000 INFO @ Mon, 03 Jun 2019 05:13:05: 28000000 INFO @ Mon, 03 Jun 2019 05:13:05: 28000000 INFO @ Mon, 03 Jun 2019 05:13:05: 28000000 INFO @ Mon, 03 Jun 2019 05:13:16: 29000000 INFO @ Mon, 03 Jun 2019 05:13:16: 29000000 INFO @ Mon, 03 Jun 2019 05:13:16: 29000000 INFO @ Mon, 03 Jun 2019 05:13:28: 30000000 INFO @ Mon, 03 Jun 2019 05:13:28: 30000000 INFO @ Mon, 03 Jun 2019 05:13:28: 30000000 INFO @ Mon, 03 Jun 2019 05:13:39: 31000000 INFO @ Mon, 03 Jun 2019 05:13:39: 31000000 INFO @ Mon, 03 Jun 2019 05:13:39: 31000000 INFO @ Mon, 03 Jun 2019 05:13:50: 32000000 INFO @ Mon, 03 Jun 2019 05:13:50: 32000000 INFO @ Mon, 03 Jun 2019 05:13:50: 32000000 INFO @ Mon, 03 Jun 2019 05:14:01: 33000000 INFO @ Mon, 03 Jun 2019 05:14:01: 33000000 INFO @ Mon, 03 Jun 2019 05:14:01: 33000000 INFO @ Mon, 03 Jun 2019 05:14:13: 34000000 INFO @ Mon, 03 Jun 2019 05:14:13: 34000000 INFO @ Mon, 03 Jun 2019 05:14:13: 34000000 INFO @ Mon, 03 Jun 2019 05:14:24: 35000000 INFO @ Mon, 03 Jun 2019 05:14:24: 35000000 INFO @ Mon, 03 Jun 2019 05:14:24: 35000000 INFO @ Mon, 03 Jun 2019 05:14:34: 36000000 INFO @ Mon, 03 Jun 2019 05:14:34: 36000000 INFO @ Mon, 03 Jun 2019 05:14:36: 36000000 INFO @ Mon, 03 Jun 2019 05:14:44: 37000000 INFO @ Mon, 03 Jun 2019 05:14:44: 37000000 INFO @ Mon, 03 Jun 2019 05:14:49: 37000000 INFO @ Mon, 03 Jun 2019 05:14:54: 38000000 INFO @ Mon, 03 Jun 2019 05:14:54: 38000000 INFO @ Mon, 03 Jun 2019 05:15:00: 38000000 INFO @ Mon, 03 Jun 2019 05:15:05: 39000000 INFO @ Mon, 03 Jun 2019 05:15:05: 39000000 INFO @ Mon, 03 Jun 2019 05:15:11: 39000000 INFO @ Mon, 03 Jun 2019 05:15:15: 40000000 INFO @ Mon, 03 Jun 2019 05:15:15: 40000000 INFO @ Mon, 03 Jun 2019 05:15:21: 40000000 INFO @ Mon, 03 Jun 2019 05:15:25: 41000000 INFO @ Mon, 03 Jun 2019 05:15:27: 41000000 INFO @ Mon, 03 Jun 2019 05:15:30: 41000000 INFO @ Mon, 03 Jun 2019 05:15:32: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:15:32: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:15:32: #1 total tags in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:15:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:15:33: #1 tags after filtering in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:15:33: #1 finished! INFO @ Mon, 03 Jun 2019 05:15:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:15:34: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:15:34: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:15:34: #1 total tags in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:15:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:15:35: #1 tags after filtering in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:15:35: #1 finished! INFO @ Mon, 03 Jun 2019 05:15:35: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:15:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:15:36: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:15:36: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:15:36: #1 total tags in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:36: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:15:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:15:37: #2 number of paired peaks: 54 WARNING @ Mon, 03 Jun 2019 05:15:37: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:15:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:15:37: #1 tags after filtering in treatment: 41656315 INFO @ Mon, 03 Jun 2019 05:15:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:15:37: #1 finished! INFO @ Mon, 03 Jun 2019 05:15:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:15:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:15:38: #2 number of paired peaks: 54 WARNING @ Mon, 03 Jun 2019 05:15:38: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:15:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:15:41: #2 number of paired peaks: 54 WARNING @ Mon, 03 Jun 2019 05:15:41: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159168/SRX159168.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。