Job ID = 1293992 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 79,102,347 reads read : 79,102,347 reads written : 79,102,347 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:23 79102347 reads; of these: 79102347 (100.00%) were unpaired; of these: 6441299 (8.14%) aligned 0 times 53498792 (67.63%) aligned exactly 1 time 19162256 (24.22%) aligned >1 times 91.86% overall alignment rate Time searching: 00:31:23 Overall time: 00:31:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 25718225 / 72661048 = 0.3539 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:11:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:11:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:11:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:11:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:11:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:11:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:11:23: 1000000 INFO @ Mon, 03 Jun 2019 05:11:24: 1000000 INFO @ Mon, 03 Jun 2019 05:11:24: 1000000 INFO @ Mon, 03 Jun 2019 05:11:30: 2000000 INFO @ Mon, 03 Jun 2019 05:11:32: 2000000 INFO @ Mon, 03 Jun 2019 05:11:33: 2000000 INFO @ Mon, 03 Jun 2019 05:11:38: 3000000 INFO @ Mon, 03 Jun 2019 05:11:41: 3000000 INFO @ Mon, 03 Jun 2019 05:11:42: 3000000 INFO @ Mon, 03 Jun 2019 05:11:46: 4000000 INFO @ Mon, 03 Jun 2019 05:11:50: 4000000 INFO @ Mon, 03 Jun 2019 05:11:51: 4000000 INFO @ Mon, 03 Jun 2019 05:11:54: 5000000 INFO @ Mon, 03 Jun 2019 05:11:59: 5000000 INFO @ Mon, 03 Jun 2019 05:12:00: 5000000 INFO @ Mon, 03 Jun 2019 05:12:01: 6000000 INFO @ Mon, 03 Jun 2019 05:12:08: 6000000 INFO @ Mon, 03 Jun 2019 05:12:09: 6000000 INFO @ Mon, 03 Jun 2019 05:12:09: 7000000 INFO @ Mon, 03 Jun 2019 05:12:17: 8000000 INFO @ Mon, 03 Jun 2019 05:12:17: 7000000 INFO @ Mon, 03 Jun 2019 05:12:18: 7000000 INFO @ Mon, 03 Jun 2019 05:12:25: 9000000 INFO @ Mon, 03 Jun 2019 05:12:26: 8000000 INFO @ Mon, 03 Jun 2019 05:12:27: 8000000 INFO @ Mon, 03 Jun 2019 05:12:32: 10000000 INFO @ Mon, 03 Jun 2019 05:12:35: 9000000 INFO @ Mon, 03 Jun 2019 05:12:36: 9000000 INFO @ Mon, 03 Jun 2019 05:12:40: 11000000 INFO @ Mon, 03 Jun 2019 05:12:44: 10000000 INFO @ Mon, 03 Jun 2019 05:12:44: 10000000 INFO @ Mon, 03 Jun 2019 05:12:47: 12000000 INFO @ Mon, 03 Jun 2019 05:12:53: 11000000 INFO @ Mon, 03 Jun 2019 05:12:53: 11000000 INFO @ Mon, 03 Jun 2019 05:12:55: 13000000 INFO @ Mon, 03 Jun 2019 05:13:01: 12000000 INFO @ Mon, 03 Jun 2019 05:13:01: 12000000 INFO @ Mon, 03 Jun 2019 05:13:02: 14000000 INFO @ Mon, 03 Jun 2019 05:13:10: 13000000 INFO @ Mon, 03 Jun 2019 05:13:10: 15000000 INFO @ Mon, 03 Jun 2019 05:13:10: 13000000 INFO @ Mon, 03 Jun 2019 05:13:18: 16000000 INFO @ Mon, 03 Jun 2019 05:13:18: 14000000 INFO @ Mon, 03 Jun 2019 05:13:19: 14000000 INFO @ Mon, 03 Jun 2019 05:13:25: 17000000 INFO @ Mon, 03 Jun 2019 05:13:27: 15000000 INFO @ Mon, 03 Jun 2019 05:13:27: 15000000 INFO @ Mon, 03 Jun 2019 05:13:33: 18000000 INFO @ Mon, 03 Jun 2019 05:13:36: 16000000 INFO @ Mon, 03 Jun 2019 05:13:36: 16000000 INFO @ Mon, 03 Jun 2019 05:13:40: 19000000 INFO @ Mon, 03 Jun 2019 05:13:44: 17000000 INFO @ Mon, 03 Jun 2019 05:13:44: 17000000 INFO @ Mon, 03 Jun 2019 05:13:48: 20000000 INFO @ Mon, 03 Jun 2019 05:13:53: 18000000 INFO @ Mon, 03 Jun 2019 05:13:53: 18000000 INFO @ Mon, 03 Jun 2019 05:13:55: 21000000 INFO @ Mon, 03 Jun 2019 05:14:02: 19000000 INFO @ Mon, 03 Jun 2019 05:14:02: 19000000 INFO @ Mon, 03 Jun 2019 05:14:03: 22000000 INFO @ Mon, 03 Jun 2019 05:14:10: 23000000 INFO @ Mon, 03 Jun 2019 05:14:11: 20000000 INFO @ Mon, 03 Jun 2019 05:14:11: 20000000 INFO @ Mon, 03 Jun 2019 05:14:18: 24000000 INFO @ Mon, 03 Jun 2019 05:14:20: 21000000 INFO @ Mon, 03 Jun 2019 05:14:20: 21000000 INFO @ Mon, 03 Jun 2019 05:14:25: 25000000 INFO @ Mon, 03 Jun 2019 05:14:29: 22000000 INFO @ Mon, 03 Jun 2019 05:14:29: 22000000 INFO @ Mon, 03 Jun 2019 05:14:33: 26000000 INFO @ Mon, 03 Jun 2019 05:14:38: 23000000 INFO @ Mon, 03 Jun 2019 05:14:38: 23000000 INFO @ Mon, 03 Jun 2019 05:14:40: 27000000 INFO @ Mon, 03 Jun 2019 05:14:47: 24000000 INFO @ Mon, 03 Jun 2019 05:14:47: 24000000 INFO @ Mon, 03 Jun 2019 05:14:48: 28000000 INFO @ Mon, 03 Jun 2019 05:14:55: 29000000 INFO @ Mon, 03 Jun 2019 05:14:56: 25000000 INFO @ Mon, 03 Jun 2019 05:14:56: 25000000 INFO @ Mon, 03 Jun 2019 05:15:02: 30000000 INFO @ Mon, 03 Jun 2019 05:15:05: 26000000 INFO @ Mon, 03 Jun 2019 05:15:05: 26000000 INFO @ Mon, 03 Jun 2019 05:15:10: 31000000 INFO @ Mon, 03 Jun 2019 05:15:14: 27000000 INFO @ Mon, 03 Jun 2019 05:15:14: 27000000 INFO @ Mon, 03 Jun 2019 05:15:17: 32000000 INFO @ Mon, 03 Jun 2019 05:15:23: 28000000 INFO @ Mon, 03 Jun 2019 05:15:23: 28000000 INFO @ Mon, 03 Jun 2019 05:15:25: 33000000 INFO @ Mon, 03 Jun 2019 05:15:32: 29000000 INFO @ Mon, 03 Jun 2019 05:15:33: 34000000 INFO @ Mon, 03 Jun 2019 05:15:33: 29000000 INFO @ Mon, 03 Jun 2019 05:15:40: 35000000 INFO @ Mon, 03 Jun 2019 05:15:42: 30000000 INFO @ Mon, 03 Jun 2019 05:15:42: 30000000 INFO @ Mon, 03 Jun 2019 05:15:47: 36000000 INFO @ Mon, 03 Jun 2019 05:15:51: 31000000 INFO @ Mon, 03 Jun 2019 05:15:51: 31000000 INFO @ Mon, 03 Jun 2019 05:15:55: 37000000 INFO @ Mon, 03 Jun 2019 05:16:00: 32000000 INFO @ Mon, 03 Jun 2019 05:16:00: 32000000 INFO @ Mon, 03 Jun 2019 05:16:02: 38000000 INFO @ Mon, 03 Jun 2019 05:16:09: 33000000 INFO @ Mon, 03 Jun 2019 05:16:09: 33000000 INFO @ Mon, 03 Jun 2019 05:16:10: 39000000 INFO @ Mon, 03 Jun 2019 05:16:17: 40000000 INFO @ Mon, 03 Jun 2019 05:16:18: 34000000 INFO @ Mon, 03 Jun 2019 05:16:18: 34000000 INFO @ Mon, 03 Jun 2019 05:16:24: 41000000 INFO @ Mon, 03 Jun 2019 05:16:27: 35000000 INFO @ Mon, 03 Jun 2019 05:16:27: 35000000 INFO @ Mon, 03 Jun 2019 05:16:32: 42000000 INFO @ Mon, 03 Jun 2019 05:16:36: 36000000 INFO @ Mon, 03 Jun 2019 05:16:36: 36000000 INFO @ Mon, 03 Jun 2019 05:16:39: 43000000 INFO @ Mon, 03 Jun 2019 05:16:45: 37000000 INFO @ Mon, 03 Jun 2019 05:16:45: 37000000 INFO @ Mon, 03 Jun 2019 05:16:47: 44000000 INFO @ Mon, 03 Jun 2019 05:16:54: 38000000 INFO @ Mon, 03 Jun 2019 05:16:54: 38000000 INFO @ Mon, 03 Jun 2019 05:16:54: 45000000 INFO @ Mon, 03 Jun 2019 05:17:02: 46000000 INFO @ Mon, 03 Jun 2019 05:17:03: 39000000 INFO @ Mon, 03 Jun 2019 05:17:03: 39000000 INFO @ Mon, 03 Jun 2019 05:17:09: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:17:09: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:17:09: #1 total tags in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:17:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:17:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:17:10: #1 tags after filtering in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:17:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:17:10: #1 finished! INFO @ Mon, 03 Jun 2019 05:17:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:17:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:17:12: 40000000 INFO @ Mon, 03 Jun 2019 05:17:12: 40000000 INFO @ Mon, 03 Jun 2019 05:17:14: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 05:17:14: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:17:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:17:20: 41000000 INFO @ Mon, 03 Jun 2019 05:17:21: 41000000 INFO @ Mon, 03 Jun 2019 05:17:29: 42000000 INFO @ Mon, 03 Jun 2019 05:17:29: 42000000 INFO @ Mon, 03 Jun 2019 05:17:38: 43000000 INFO @ Mon, 03 Jun 2019 05:17:38: 43000000 INFO @ Mon, 03 Jun 2019 05:17:47: 44000000 INFO @ Mon, 03 Jun 2019 05:17:47: 44000000 INFO @ Mon, 03 Jun 2019 05:17:55: 45000000 INFO @ Mon, 03 Jun 2019 05:17:55: 45000000 INFO @ Mon, 03 Jun 2019 05:18:04: 46000000 INFO @ Mon, 03 Jun 2019 05:18:04: 46000000 INFO @ Mon, 03 Jun 2019 05:18:13: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:18:13: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:18:13: #1 total tags in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:18:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:18:13: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 05:18:13: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 05:18:13: #1 total tags in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:18:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:18:13: #1 tags after filtering in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:18:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:18:13: #1 finished! INFO @ Mon, 03 Jun 2019 05:18:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:18:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:18:14: #1 tags after filtering in treatment: 46942823 INFO @ Mon, 03 Jun 2019 05:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:18:14: #1 finished! INFO @ Mon, 03 Jun 2019 05:18:14: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:18:17: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 05:18:17: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:18:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:18:18: #2 number of paired peaks: 5 WARNING @ Mon, 03 Jun 2019 05:18:18: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:18:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159164/SRX159164.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。