Job ID = 1293991 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 74,673,445 reads read : 74,673,445 reads written : 74,673,445 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:38 74673445 reads; of these: 74673445 (100.00%) were unpaired; of these: 6129469 (8.21%) aligned 0 times 47450465 (63.54%) aligned exactly 1 time 21093511 (28.25%) aligned >1 times 91.79% overall alignment rate Time searching: 00:30:38 Overall time: 00:30:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13324681 / 68543976 = 0.1944 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:40:44: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:40:44: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:40:44: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:40:44: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:40:44: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:40:44: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:40:52: 1000000 INFO @ Mon, 03 Jun 2019 04:40:52: 1000000 INFO @ Mon, 03 Jun 2019 04:40:54: 1000000 INFO @ Mon, 03 Jun 2019 04:40:59: 2000000 INFO @ Mon, 03 Jun 2019 04:41:00: 2000000 INFO @ Mon, 03 Jun 2019 04:41:04: 2000000 INFO @ Mon, 03 Jun 2019 04:41:07: 3000000 INFO @ Mon, 03 Jun 2019 04:41:08: 3000000 INFO @ Mon, 03 Jun 2019 04:41:13: 3000000 INFO @ Mon, 03 Jun 2019 04:41:14: 4000000 INFO @ Mon, 03 Jun 2019 04:41:16: 4000000 INFO @ Mon, 03 Jun 2019 04:41:21: 5000000 INFO @ Mon, 03 Jun 2019 04:41:23: 4000000 INFO @ Mon, 03 Jun 2019 04:41:23: 5000000 INFO @ Mon, 03 Jun 2019 04:41:28: 6000000 INFO @ Mon, 03 Jun 2019 04:41:31: 6000000 INFO @ Mon, 03 Jun 2019 04:41:32: 5000000 INFO @ Mon, 03 Jun 2019 04:41:36: 7000000 INFO @ Mon, 03 Jun 2019 04:41:39: 7000000 INFO @ Mon, 03 Jun 2019 04:41:42: 6000000 INFO @ Mon, 03 Jun 2019 04:41:43: 8000000 INFO @ Mon, 03 Jun 2019 04:41:46: 8000000 INFO @ Mon, 03 Jun 2019 04:41:50: 9000000 INFO @ Mon, 03 Jun 2019 04:41:52: 7000000 INFO @ Mon, 03 Jun 2019 04:41:54: 9000000 INFO @ Mon, 03 Jun 2019 04:41:57: 10000000 INFO @ Mon, 03 Jun 2019 04:42:01: 8000000 INFO @ Mon, 03 Jun 2019 04:42:02: 10000000 INFO @ Mon, 03 Jun 2019 04:42:04: 11000000 INFO @ Mon, 03 Jun 2019 04:42:09: 11000000 INFO @ Mon, 03 Jun 2019 04:42:10: 9000000 INFO @ Mon, 03 Jun 2019 04:42:11: 12000000 INFO @ Mon, 03 Jun 2019 04:42:17: 12000000 INFO @ Mon, 03 Jun 2019 04:42:19: 13000000 INFO @ Mon, 03 Jun 2019 04:42:19: 10000000 INFO @ Mon, 03 Jun 2019 04:42:25: 13000000 INFO @ Mon, 03 Jun 2019 04:42:26: 14000000 INFO @ Mon, 03 Jun 2019 04:42:29: 11000000 INFO @ Mon, 03 Jun 2019 04:42:32: 14000000 INFO @ Mon, 03 Jun 2019 04:42:33: 15000000 INFO @ Mon, 03 Jun 2019 04:42:39: 12000000 INFO @ Mon, 03 Jun 2019 04:42:40: 15000000 INFO @ Mon, 03 Jun 2019 04:42:40: 16000000 INFO @ Mon, 03 Jun 2019 04:42:47: 17000000 INFO @ Mon, 03 Jun 2019 04:42:48: 16000000 INFO @ Mon, 03 Jun 2019 04:42:48: 13000000 INFO @ Mon, 03 Jun 2019 04:42:54: 18000000 INFO @ Mon, 03 Jun 2019 04:42:55: 17000000 INFO @ Mon, 03 Jun 2019 04:42:57: 14000000 INFO @ Mon, 03 Jun 2019 04:43:02: 19000000 INFO @ Mon, 03 Jun 2019 04:43:03: 18000000 INFO @ Mon, 03 Jun 2019 04:43:07: 15000000 INFO @ Mon, 03 Jun 2019 04:43:09: 20000000 INFO @ Mon, 03 Jun 2019 04:43:11: 19000000 INFO @ Mon, 03 Jun 2019 04:43:16: 16000000 INFO @ Mon, 03 Jun 2019 04:43:16: 21000000 INFO @ Mon, 03 Jun 2019 04:43:18: 20000000 INFO @ Mon, 03 Jun 2019 04:43:23: 22000000 INFO @ Mon, 03 Jun 2019 04:43:26: 21000000 INFO @ Mon, 03 Jun 2019 04:43:26: 17000000 INFO @ Mon, 03 Jun 2019 04:43:30: 23000000 INFO @ Mon, 03 Jun 2019 04:43:33: 22000000 INFO @ Mon, 03 Jun 2019 04:43:36: 18000000 INFO @ Mon, 03 Jun 2019 04:43:37: 24000000 INFO @ Mon, 03 Jun 2019 04:43:41: 23000000 INFO @ Mon, 03 Jun 2019 04:43:45: 25000000 INFO @ Mon, 03 Jun 2019 04:43:45: 19000000 INFO @ Mon, 03 Jun 2019 04:43:49: 24000000 INFO @ Mon, 03 Jun 2019 04:43:52: 26000000 INFO @ Mon, 03 Jun 2019 04:43:54: 20000000 INFO @ Mon, 03 Jun 2019 04:43:56: 25000000 INFO @ Mon, 03 Jun 2019 04:43:59: 27000000 INFO @ Mon, 03 Jun 2019 04:44:03: 21000000 INFO @ Mon, 03 Jun 2019 04:44:04: 26000000 INFO @ Mon, 03 Jun 2019 04:44:06: 28000000 INFO @ Mon, 03 Jun 2019 04:44:12: 27000000 INFO @ Mon, 03 Jun 2019 04:44:13: 22000000 INFO @ Mon, 03 Jun 2019 04:44:13: 29000000 INFO @ Mon, 03 Jun 2019 04:44:19: 28000000 INFO @ Mon, 03 Jun 2019 04:44:20: 30000000 INFO @ Mon, 03 Jun 2019 04:44:23: 23000000 INFO @ Mon, 03 Jun 2019 04:44:27: 29000000 INFO @ Mon, 03 Jun 2019 04:44:28: 31000000 INFO @ Mon, 03 Jun 2019 04:44:32: 24000000 INFO @ Mon, 03 Jun 2019 04:44:35: 30000000 INFO @ Mon, 03 Jun 2019 04:44:35: 32000000 INFO @ Mon, 03 Jun 2019 04:44:41: 25000000 INFO @ Mon, 03 Jun 2019 04:44:42: 33000000 INFO @ Mon, 03 Jun 2019 04:44:42: 31000000 INFO @ Mon, 03 Jun 2019 04:44:49: 34000000 INFO @ Mon, 03 Jun 2019 04:44:50: 32000000 INFO @ Mon, 03 Jun 2019 04:44:51: 26000000 INFO @ Mon, 03 Jun 2019 04:44:56: 35000000 INFO @ Mon, 03 Jun 2019 04:44:57: 33000000 INFO @ Mon, 03 Jun 2019 04:45:00: 27000000 INFO @ Mon, 03 Jun 2019 04:45:03: 36000000 INFO @ Mon, 03 Jun 2019 04:45:05: 34000000 INFO @ Mon, 03 Jun 2019 04:45:09: 28000000 INFO @ Mon, 03 Jun 2019 04:45:11: 37000000 INFO @ Mon, 03 Jun 2019 04:45:13: 35000000 INFO @ Mon, 03 Jun 2019 04:45:18: 38000000 INFO @ Mon, 03 Jun 2019 04:45:19: 29000000 INFO @ Mon, 03 Jun 2019 04:45:20: 36000000 INFO @ Mon, 03 Jun 2019 04:45:25: 39000000 INFO @ Mon, 03 Jun 2019 04:45:28: 37000000 INFO @ Mon, 03 Jun 2019 04:45:28: 30000000 INFO @ Mon, 03 Jun 2019 04:45:32: 40000000 INFO @ Mon, 03 Jun 2019 04:45:35: 38000000 INFO @ Mon, 03 Jun 2019 04:45:38: 31000000 INFO @ Mon, 03 Jun 2019 04:45:39: 41000000 INFO @ Mon, 03 Jun 2019 04:45:43: 39000000 INFO @ Mon, 03 Jun 2019 04:45:46: 42000000 INFO @ Mon, 03 Jun 2019 04:45:47: 32000000 INFO @ Mon, 03 Jun 2019 04:45:51: 40000000 INFO @ Mon, 03 Jun 2019 04:45:53: 43000000 INFO @ Mon, 03 Jun 2019 04:45:56: 33000000 INFO @ Mon, 03 Jun 2019 04:45:58: 41000000 INFO @ Mon, 03 Jun 2019 04:46:00: 44000000 INFO @ Mon, 03 Jun 2019 04:46:05: 34000000 INFO @ Mon, 03 Jun 2019 04:46:06: 42000000 INFO @ Mon, 03 Jun 2019 04:46:07: 45000000 INFO @ Mon, 03 Jun 2019 04:46:14: 43000000 INFO @ Mon, 03 Jun 2019 04:46:15: 46000000 INFO @ Mon, 03 Jun 2019 04:46:15: 35000000 INFO @ Mon, 03 Jun 2019 04:46:21: 44000000 INFO @ Mon, 03 Jun 2019 04:46:22: 47000000 INFO @ Mon, 03 Jun 2019 04:46:25: 36000000 INFO @ Mon, 03 Jun 2019 04:46:29: 45000000 INFO @ Mon, 03 Jun 2019 04:46:29: 48000000 INFO @ Mon, 03 Jun 2019 04:46:34: 37000000 INFO @ Mon, 03 Jun 2019 04:46:36: 49000000 INFO @ Mon, 03 Jun 2019 04:46:36: 46000000 INFO @ Mon, 03 Jun 2019 04:46:43: 50000000 INFO @ Mon, 03 Jun 2019 04:46:43: 38000000 INFO @ Mon, 03 Jun 2019 04:46:44: 47000000 INFO @ Mon, 03 Jun 2019 04:46:50: 51000000 INFO @ Mon, 03 Jun 2019 04:46:51: 48000000 INFO @ Mon, 03 Jun 2019 04:46:52: 39000000 INFO @ Mon, 03 Jun 2019 04:46:57: 52000000 INFO @ Mon, 03 Jun 2019 04:46:59: 49000000 INFO @ Mon, 03 Jun 2019 04:47:01: 40000000 INFO @ Mon, 03 Jun 2019 04:47:04: 53000000 INFO @ Mon, 03 Jun 2019 04:47:07: 50000000 INFO @ Mon, 03 Jun 2019 04:47:11: 54000000 INFO @ Mon, 03 Jun 2019 04:47:11: 41000000 INFO @ Mon, 03 Jun 2019 04:47:14: 51000000 INFO @ Mon, 03 Jun 2019 04:47:19: 55000000 INFO @ Mon, 03 Jun 2019 04:47:21: 42000000 INFO @ Mon, 03 Jun 2019 04:47:21: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:47:21: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:47:21: #1 total tags in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:47:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:47:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:47:22: 52000000 INFO @ Mon, 03 Jun 2019 04:47:22: #1 tags after filtering in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:47:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:47:22: #1 finished! INFO @ Mon, 03 Jun 2019 04:47:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:47:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:47:27: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:47:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:47:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:47:29: 53000000 INFO @ Mon, 03 Jun 2019 04:47:30: 43000000 INFO @ Mon, 03 Jun 2019 04:47:37: 54000000 INFO @ Mon, 03 Jun 2019 04:47:39: 44000000 INFO @ Mon, 03 Jun 2019 04:47:45: 55000000 INFO @ Mon, 03 Jun 2019 04:47:47: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:47:47: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:47:47: #1 total tags in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:47:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:47:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:47:48: 45000000 INFO @ Mon, 03 Jun 2019 04:47:48: #1 tags after filtering in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:47:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:47:48: #1 finished! INFO @ Mon, 03 Jun 2019 04:47:48: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:47:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:47:53: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:47:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:47:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:47:57: 46000000 INFO @ Mon, 03 Jun 2019 04:48:06: 47000000 INFO @ Mon, 03 Jun 2019 04:48:15: 48000000 INFO @ Mon, 03 Jun 2019 04:48:24: 49000000 INFO @ Mon, 03 Jun 2019 04:48:33: 50000000 INFO @ Mon, 03 Jun 2019 04:48:42: 51000000 INFO @ Mon, 03 Jun 2019 04:48:51: 52000000 INFO @ Mon, 03 Jun 2019 04:49:00: 53000000 INFO @ Mon, 03 Jun 2019 04:49:09: 54000000 INFO @ Mon, 03 Jun 2019 04:49:18: 55000000 INFO @ Mon, 03 Jun 2019 04:49:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:49:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:49:20: #1 total tags in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:49:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:49:22: #1 tags after filtering in treatment: 55219295 INFO @ Mon, 03 Jun 2019 04:49:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:49:22: #1 finished! INFO @ Mon, 03 Jun 2019 04:49:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:49:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:49:26: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:49:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:49:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159163/SRX159163.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。