Job ID = 16440188 SRX = SRX15369008 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T08:32:28 prefetch.2.10.7: 1) Downloading 'SRR19308737'... 2022-08-02T08:32:28 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:33:07 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:33:07 prefetch.2.10.7: 1) 'SRR19308737' was downloaded successfully 2022-08-02T08:33:07 prefetch.2.10.7: 'SRR19308737' has 0 unresolved dependencies Read 19376276 spots for SRR19308737/SRR19308737.sra Written 19376276 spots for SRR19308737/SRR19308737.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440777 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:05 19376276 reads; of these: 19376276 (100.00%) were unpaired; of these: 647964 (3.34%) aligned 0 times 11758815 (60.69%) aligned exactly 1 time 6969497 (35.97%) aligned >1 times 96.66% overall alignment rate Time searching: 00:14:05 Overall time: 00:14:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3746977 / 18728312 = 0.2001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:56:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:56:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:56:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:56:12: 1000000 INFO @ Tue, 02 Aug 2022 17:56:20: 2000000 INFO @ Tue, 02 Aug 2022 17:56:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:56:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:56:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:56:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:56:36: 4000000 INFO @ Tue, 02 Aug 2022 17:56:41: 1000000 INFO @ Tue, 02 Aug 2022 17:56:44: 5000000 INFO @ Tue, 02 Aug 2022 17:56:49: 2000000 INFO @ Tue, 02 Aug 2022 17:56:52: 6000000 INFO @ Tue, 02 Aug 2022 17:56:56: 3000000 INFO @ Tue, 02 Aug 2022 17:57:00: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:57:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:57:03: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:57:03: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:57:04: 4000000 INFO @ Tue, 02 Aug 2022 17:57:08: 8000000 INFO @ Tue, 02 Aug 2022 17:57:11: 1000000 INFO @ Tue, 02 Aug 2022 17:57:12: 5000000 INFO @ Tue, 02 Aug 2022 17:57:17: 9000000 INFO @ Tue, 02 Aug 2022 17:57:19: 2000000 INFO @ Tue, 02 Aug 2022 17:57:20: 6000000 INFO @ Tue, 02 Aug 2022 17:57:25: 10000000 INFO @ Tue, 02 Aug 2022 17:57:27: 3000000 INFO @ Tue, 02 Aug 2022 17:57:28: 7000000 INFO @ Tue, 02 Aug 2022 17:57:34: 11000000 INFO @ Tue, 02 Aug 2022 17:57:36: 4000000 INFO @ Tue, 02 Aug 2022 17:57:37: 8000000 INFO @ Tue, 02 Aug 2022 17:57:42: 12000000 INFO @ Tue, 02 Aug 2022 17:57:44: 5000000 INFO @ Tue, 02 Aug 2022 17:57:45: 9000000 INFO @ Tue, 02 Aug 2022 17:57:50: 13000000 INFO @ Tue, 02 Aug 2022 17:57:52: 6000000 INFO @ Tue, 02 Aug 2022 17:57:53: 10000000 INFO @ Tue, 02 Aug 2022 17:58:00: 14000000 INFO @ Tue, 02 Aug 2022 17:58:00: 7000000 INFO @ Tue, 02 Aug 2022 17:58:01: 11000000 INFO @ Tue, 02 Aug 2022 17:58:08: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 17:58:08: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 17:58:08: #1 total tags in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:58:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:58:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:58:08: 8000000 INFO @ Tue, 02 Aug 2022 17:58:08: #1 tags after filtering in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:58:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:58:08: #1 finished! INFO @ Tue, 02 Aug 2022 17:58:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:58:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:58:09: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 17:58:09: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:58:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:58:09: 12000000 INFO @ Tue, 02 Aug 2022 17:58:16: 9000000 INFO @ Tue, 02 Aug 2022 17:58:17: 13000000 INFO @ Tue, 02 Aug 2022 17:58:24: 10000000 INFO @ Tue, 02 Aug 2022 17:58:25: 14000000 INFO @ Tue, 02 Aug 2022 17:58:32: 11000000 INFO @ Tue, 02 Aug 2022 17:58:32: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 17:58:32: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 17:58:32: #1 total tags in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:58:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:58:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:58:33: #1 tags after filtering in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:58:33: #1 finished! INFO @ Tue, 02 Aug 2022 17:58:33: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:58:34: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 17:58:34: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:58:39: 12000000 INFO @ Tue, 02 Aug 2022 17:58:47: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:58:55: 14000000 INFO @ Tue, 02 Aug 2022 17:59:02: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 17:59:02: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 17:59:02: #1 total tags in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:59:02: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:59:02: #1 tags after filtering in treatment: 14981335 INFO @ Tue, 02 Aug 2022 17:59:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:59:02: #1 finished! INFO @ Tue, 02 Aug 2022 17:59:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:59:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:59:03: #2 number of paired peaks: 88 WARNING @ Tue, 02 Aug 2022 17:59:03: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:59:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15369008/SRX15369008.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。