Job ID = 16438999 SRX = SRX15312100 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:21:17 prefetch.2.10.7: 1) Downloading 'SRR19251483'... 2022-08-02T05:21:17 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:22:27 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:22:27 prefetch.2.10.7: 1) 'SRR19251483' was downloaded successfully 2022-08-02T05:22:27 prefetch.2.10.7: 'SRR19251483' has 0 unresolved dependencies Read 40258128 spots for SRR19251483/SRR19251483.sra Written 40258128 spots for SRR19251483/SRR19251483.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439177 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:02 Multiseed full-index search: 00:15:47 40258128 reads; of these: 40258128 (100.00%) were unpaired; of these: 924005 (2.30%) aligned 0 times 31543142 (78.35%) aligned exactly 1 time 7790981 (19.35%) aligned >1 times 97.70% overall alignment rate Time searching: 00:15:49 Overall time: 00:15:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4353845 / 39334123 = 0.1107 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:54:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:54:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:54:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:54:09: 1000000 INFO @ Tue, 02 Aug 2022 14:54:16: 2000000 INFO @ Tue, 02 Aug 2022 14:54:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:54:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:54:31: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:54:31: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:54:31: 4000000 INFO @ Tue, 02 Aug 2022 14:54:39: 1000000 INFO @ Tue, 02 Aug 2022 14:54:40: 5000000 INFO @ Tue, 02 Aug 2022 14:54:47: 2000000 INFO @ Tue, 02 Aug 2022 14:54:48: 6000000 INFO @ Tue, 02 Aug 2022 14:54:54: 3000000 INFO @ Tue, 02 Aug 2022 14:54:56: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:55:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:55:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:55:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:55:02: 4000000 INFO @ Tue, 02 Aug 2022 14:55:03: 8000000 INFO @ Tue, 02 Aug 2022 14:55:09: 5000000 INFO @ Tue, 02 Aug 2022 14:55:10: 1000000 INFO @ Tue, 02 Aug 2022 14:55:12: 9000000 INFO @ Tue, 02 Aug 2022 14:55:16: 6000000 INFO @ Tue, 02 Aug 2022 14:55:19: 2000000 INFO @ Tue, 02 Aug 2022 14:55:20: 10000000 INFO @ Tue, 02 Aug 2022 14:55:24: 7000000 INFO @ Tue, 02 Aug 2022 14:55:28: 3000000 INFO @ Tue, 02 Aug 2022 14:55:29: 11000000 INFO @ Tue, 02 Aug 2022 14:55:31: 8000000 INFO @ Tue, 02 Aug 2022 14:55:37: 4000000 INFO @ Tue, 02 Aug 2022 14:55:38: 12000000 INFO @ Tue, 02 Aug 2022 14:55:38: 9000000 INFO @ Tue, 02 Aug 2022 14:55:46: 10000000 INFO @ Tue, 02 Aug 2022 14:55:46: 5000000 INFO @ Tue, 02 Aug 2022 14:55:48: 13000000 INFO @ Tue, 02 Aug 2022 14:55:53: 11000000 INFO @ Tue, 02 Aug 2022 14:55:56: 6000000 INFO @ Tue, 02 Aug 2022 14:55:57: 14000000 INFO @ Tue, 02 Aug 2022 14:56:00: 12000000 INFO @ Tue, 02 Aug 2022 14:56:05: 7000000 INFO @ Tue, 02 Aug 2022 14:56:06: 15000000 INFO @ Tue, 02 Aug 2022 14:56:07: 13000000 INFO @ Tue, 02 Aug 2022 14:56:14: 8000000 INFO @ Tue, 02 Aug 2022 14:56:15: 14000000 INFO @ Tue, 02 Aug 2022 14:56:15: 16000000 INFO @ Tue, 02 Aug 2022 14:56:22: 15000000 INFO @ Tue, 02 Aug 2022 14:56:23: 9000000 INFO @ Tue, 02 Aug 2022 14:56:24: 17000000 INFO @ Tue, 02 Aug 2022 14:56:29: 16000000 INFO @ Tue, 02 Aug 2022 14:56:32: 10000000 INFO @ Tue, 02 Aug 2022 14:56:34: 18000000 INFO @ Tue, 02 Aug 2022 14:56:36: 17000000 INFO @ Tue, 02 Aug 2022 14:56:41: 11000000 INFO @ Tue, 02 Aug 2022 14:56:43: 18000000 INFO @ Tue, 02 Aug 2022 14:56:43: 19000000 INFO @ Tue, 02 Aug 2022 14:56:50: 19000000 INFO @ Tue, 02 Aug 2022 14:56:50: 12000000 INFO @ Tue, 02 Aug 2022 14:56:53: 20000000 INFO @ Tue, 02 Aug 2022 14:56:57: 20000000 INFO @ Tue, 02 Aug 2022 14:57:00: 13000000 INFO @ Tue, 02 Aug 2022 14:57:02: 21000000 INFO @ Tue, 02 Aug 2022 14:57:04: 21000000 INFO @ Tue, 02 Aug 2022 14:57:09: 14000000 INFO @ Tue, 02 Aug 2022 14:57:12: 22000000 INFO @ Tue, 02 Aug 2022 14:57:12: 22000000 INFO @ Tue, 02 Aug 2022 14:57:18: 15000000 INFO @ Tue, 02 Aug 2022 14:57:20: 23000000 INFO @ Tue, 02 Aug 2022 14:57:21: 23000000 INFO @ Tue, 02 Aug 2022 14:57:27: 16000000 INFO @ Tue, 02 Aug 2022 14:57:29: 24000000 INFO @ Tue, 02 Aug 2022 14:57:29: 24000000 INFO @ Tue, 02 Aug 2022 14:57:36: 17000000 INFO @ Tue, 02 Aug 2022 14:57:36: 25000000 INFO @ Tue, 02 Aug 2022 14:57:38: 25000000 INFO @ Tue, 02 Aug 2022 14:57:43: 26000000 INFO @ Tue, 02 Aug 2022 14:57:45: 18000000 INFO @ Tue, 02 Aug 2022 14:57:47: 26000000 INFO @ Tue, 02 Aug 2022 14:57:50: 27000000 INFO @ Tue, 02 Aug 2022 14:57:54: 19000000 INFO @ Tue, 02 Aug 2022 14:57:57: 27000000 INFO @ Tue, 02 Aug 2022 14:57:57: 28000000 INFO @ Tue, 02 Aug 2022 14:58:04: 20000000 INFO @ Tue, 02 Aug 2022 14:58:04: 29000000 INFO @ Tue, 02 Aug 2022 14:58:06: 28000000 INFO @ Tue, 02 Aug 2022 14:58:12: 30000000 INFO @ Tue, 02 Aug 2022 14:58:13: 21000000 INFO @ Tue, 02 Aug 2022 14:58:16: 29000000 INFO @ Tue, 02 Aug 2022 14:58:20: 31000000 INFO @ Tue, 02 Aug 2022 14:58:23: 22000000 INFO @ Tue, 02 Aug 2022 14:58:25: 30000000 INFO @ Tue, 02 Aug 2022 14:58:28: 32000000 INFO @ Tue, 02 Aug 2022 14:58:32: 23000000 INFO @ Tue, 02 Aug 2022 14:58:35: 31000000 INFO @ Tue, 02 Aug 2022 14:58:36: 33000000 INFO @ Tue, 02 Aug 2022 14:58:42: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:58:44: 34000000 INFO @ Tue, 02 Aug 2022 14:58:44: 32000000 INFO @ Tue, 02 Aug 2022 14:58:51: 25000000 INFO @ Tue, 02 Aug 2022 14:58:52: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:58:52: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:58:52: #1 total tags in treatment: 34980278 INFO @ Tue, 02 Aug 2022 14:58:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:58:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:58:53: #1 tags after filtering in treatment: 34980278 INFO @ Tue, 02 Aug 2022 14:58:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:58:53: #1 finished! INFO @ Tue, 02 Aug 2022 14:58:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:58:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:58:54: 33000000 INFO @ Tue, 02 Aug 2022 14:58:55: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 14:58:55: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:58:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:59:01: 26000000 INFO @ Tue, 02 Aug 2022 14:59:03: 34000000 INFO @ Tue, 02 Aug 2022 14:59:10: 27000000 INFO @ Tue, 02 Aug 2022 14:59:12: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:59:12: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:59:12: #1 total tags in treatment: 34980278 INFO @ Tue, 02 Aug 2022 14:59:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:59:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:59:13: #1 tags after filtering in treatment: 34980278 INFO @ Tue, 02 Aug 2022 14:59:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:59:13: #1 finished! INFO @ Tue, 02 Aug 2022 14:59:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:59:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:59:15: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 14:59:15: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:59:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:59:19: 28000000 INFO @ Tue, 02 Aug 2022 14:59:28: 29000000 INFO @ Tue, 02 Aug 2022 14:59:37: 30000000 INFO @ Tue, 02 Aug 2022 14:59:46: 31000000 INFO @ Tue, 02 Aug 2022 14:59:53: 32000000 INFO @ Tue, 02 Aug 2022 15:00:01: 33000000 INFO @ Tue, 02 Aug 2022 15:00:08: 34000000 INFO @ Tue, 02 Aug 2022 15:00:16: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:00:16: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:00:16: #1 total tags in treatment: 34980278 INFO @ Tue, 02 Aug 2022 15:00:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:00:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:00:16: #1 tags after filtering in treatment: 34980278 INFO @ Tue, 02 Aug 2022 15:00:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:00:16: #1 finished! INFO @ Tue, 02 Aug 2022 15:00:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:00:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:00:18: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 15:00:18: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:00:18: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312100/SRX15312100.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling