Job ID = 16439320 SRX = SRX15206498 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:53:22 prefetch.2.10.7: 1) Downloading 'SRR19139294'... 2022-08-02T05:53:22 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:53:32 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:53:33 prefetch.2.10.7: 'SRR19139294' is valid 2022-08-02T05:53:33 prefetch.2.10.7: 1) 'SRR19139294' was downloaded successfully 2022-08-02T05:53:33 prefetch.2.10.7: 'SRR19139294' has 0 unresolved dependencies Read 4965525 spots for SRR19139294/SRR19139294.sra Written 4965525 spots for SRR19139294/SRR19139294.sra 2022-08-02T05:53:58 prefetch.2.10.7: 1) Downloading 'SRR19139356'... 2022-08-02T05:53:58 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:54:09 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:54:10 prefetch.2.10.7: 'SRR19139356' is valid 2022-08-02T05:54:10 prefetch.2.10.7: 1) 'SRR19139356' was downloaded successfully 2022-08-02T05:54:10 prefetch.2.10.7: 'SRR19139356' has 0 unresolved dependencies Read 4965525 spots for SRR19139356/SRR19139356.sra Written 4965525 spots for SRR19139356/SRR19139356.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439379 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:17 9931050 reads; of these: 9931050 (100.00%) were unpaired; of these: 290107 (2.92%) aligned 0 times 8418059 (84.77%) aligned exactly 1 time 1222884 (12.31%) aligned >1 times 97.08% overall alignment rate Time searching: 00:04:17 Overall time: 00:04:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 490003 / 9640943 = 0.0508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:03:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:03:27: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:03:27: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:03:34: 1000000 INFO @ Tue, 02 Aug 2022 15:03:41: 2000000 INFO @ Tue, 02 Aug 2022 15:03:49: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:03:56: 4000000 INFO @ Tue, 02 Aug 2022 15:03:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:03:57: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:03:57: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:04:03: 5000000 INFO @ Tue, 02 Aug 2022 15:04:04: 1000000 INFO @ Tue, 02 Aug 2022 15:04:10: 6000000 INFO @ Tue, 02 Aug 2022 15:04:11: 2000000 INFO @ Tue, 02 Aug 2022 15:04:17: 7000000 INFO @ Tue, 02 Aug 2022 15:04:19: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:04:24: 8000000 INFO @ Tue, 02 Aug 2022 15:04:26: 4000000 INFO @ Tue, 02 Aug 2022 15:04:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:04:27: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:04:27: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:04:32: 9000000 INFO @ Tue, 02 Aug 2022 15:04:34: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:04:34: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:04:34: #1 total tags in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:04:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:04:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:04:34: #1 tags after filtering in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:04:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:04:34: #1 finished! INFO @ Tue, 02 Aug 2022 15:04:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:04:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:04:34: 5000000 INFO @ Tue, 02 Aug 2022 15:04:35: #2 number of paired peaks: 91 WARNING @ Tue, 02 Aug 2022 15:04:35: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:04:35: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 15:04:35: 1000000 cut: /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:04:42: 6000000 INFO @ Tue, 02 Aug 2022 15:04:42: 2000000 INFO @ Tue, 02 Aug 2022 15:04:50: 7000000 INFO @ Tue, 02 Aug 2022 15:04:50: 3000000 INFO @ Tue, 02 Aug 2022 15:04:57: 4000000 INFO @ Tue, 02 Aug 2022 15:04:57: 8000000 INFO @ Tue, 02 Aug 2022 15:05:04: 9000000 INFO @ Tue, 02 Aug 2022 15:05:05: 5000000 INFO @ Tue, 02 Aug 2022 15:05:05: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:05:06: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:05:06: #1 total tags in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:05:06: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:06: #1 tags after filtering in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:05:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:05:06: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:06: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:06: #2 number of paired peaks: 91 WARNING @ Tue, 02 Aug 2022 15:05:06: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:05:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:05:11: 6000000 INFO @ Tue, 02 Aug 2022 15:05:18: 7000000 INFO @ Tue, 02 Aug 2022 15:05:25: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:05:33: 9000000 INFO @ Tue, 02 Aug 2022 15:05:34: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:05:34: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:05:34: #1 total tags in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:05:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:35: #1 tags after filtering in treatment: 9150940 INFO @ Tue, 02 Aug 2022 15:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:05:35: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:35: #2 number of paired peaks: 91 WARNING @ Tue, 02 Aug 2022 15:05:35: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:05:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206498/SRX15206498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。