Job ID = 16439200 SRX = SRX15206476 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:47:21 prefetch.2.10.7: 1) Downloading 'SRR19139329'... 2022-08-02T05:47:21 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:48:33 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:48:33 prefetch.2.10.7: 1) 'SRR19139329' was downloaded successfully 2022-08-02T05:48:33 prefetch.2.10.7: 'SRR19139329' has 0 unresolved dependencies Read 19524057 spots for SRR19139329/SRR19139329.sra Written 19524057 spots for SRR19139329/SRR19139329.sra 2022-08-02T05:50:35 prefetch.2.10.7: 1) Downloading 'SRR19139330'... 2022-08-02T05:50:35 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:51:43 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:51:43 prefetch.2.10.7: 1) 'SRR19139330' was downloaded successfully 2022-08-02T05:51:43 prefetch.2.10.7: 'SRR19139330' has 0 unresolved dependencies Read 19524057 spots for SRR19139330/SRR19139330.sra Written 19524057 spots for SRR19139330/SRR19139330.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439572 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:55 39048114 reads; of these: 39048114 (100.00%) were unpaired; of these: 1879224 (4.81%) aligned 0 times 31238469 (80.00%) aligned exactly 1 time 5930421 (15.19%) aligned >1 times 95.19% overall alignment rate Time searching: 00:34:56 Overall time: 00:34:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 11846296 / 37168890 = 0.3187 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:52:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:52:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:52:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:26: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:52:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:52:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:52:43: 2000000 INFO @ Tue, 02 Aug 2022 15:52:54: 1000000 INFO @ Tue, 02 Aug 2022 15:53:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:53:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:53:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:53:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:53:12: 2000000 INFO @ Tue, 02 Aug 2022 15:53:17: 4000000 INFO @ Tue, 02 Aug 2022 15:53:23: 1000000 INFO @ Tue, 02 Aug 2022 15:53:28: 3000000 INFO @ Tue, 02 Aug 2022 15:53:34: 5000000 INFO @ Tue, 02 Aug 2022 15:53:40: 2000000 INFO @ Tue, 02 Aug 2022 15:53:46: 4000000 INFO @ Tue, 02 Aug 2022 15:53:52: 6000000 INFO @ Tue, 02 Aug 2022 15:53:58: 3000000 INFO @ Tue, 02 Aug 2022 15:54:02: 5000000 INFO @ Tue, 02 Aug 2022 15:54:09: 7000000 INFO @ Tue, 02 Aug 2022 15:54:15: 4000000 INFO @ Tue, 02 Aug 2022 15:54:19: 6000000 INFO @ Tue, 02 Aug 2022 15:54:25: 8000000 INFO @ Tue, 02 Aug 2022 15:54:33: 5000000 INFO @ Tue, 02 Aug 2022 15:54:39: 7000000 INFO @ Tue, 02 Aug 2022 15:54:42: 9000000 INFO @ Tue, 02 Aug 2022 15:54:50: 6000000 INFO @ Tue, 02 Aug 2022 15:54:55: 8000000 INFO @ Tue, 02 Aug 2022 15:55:00: 10000000 INFO @ Tue, 02 Aug 2022 15:55:07: 7000000 INFO @ Tue, 02 Aug 2022 15:55:12: 9000000 INFO @ Tue, 02 Aug 2022 15:55:15: 11000000 INFO @ Tue, 02 Aug 2022 15:55:23: 8000000 INFO @ Tue, 02 Aug 2022 15:55:29: 10000000 INFO @ Tue, 02 Aug 2022 15:55:34: 12000000 INFO @ Tue, 02 Aug 2022 15:55:41: 9000000 INFO @ Tue, 02 Aug 2022 15:55:48: 11000000 INFO @ Tue, 02 Aug 2022 15:55:52: 13000000 INFO @ Tue, 02 Aug 2022 15:55:58: 10000000 INFO @ Tue, 02 Aug 2022 15:56:03: 12000000 INFO @ Tue, 02 Aug 2022 15:56:08: 14000000 INFO @ Tue, 02 Aug 2022 15:56:14: 11000000 INFO @ Tue, 02 Aug 2022 15:56:20: 13000000 INFO @ Tue, 02 Aug 2022 15:56:24: 15000000 INFO @ Tue, 02 Aug 2022 15:56:30: 12000000 INFO @ Tue, 02 Aug 2022 15:56:35: 14000000 INFO @ Tue, 02 Aug 2022 15:56:39: 16000000 INFO @ Tue, 02 Aug 2022 15:56:48: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:56:51: 15000000 INFO @ Tue, 02 Aug 2022 15:56:54: 17000000 INFO @ Tue, 02 Aug 2022 15:57:04: 14000000 INFO @ Tue, 02 Aug 2022 15:57:06: 16000000 INFO @ Tue, 02 Aug 2022 15:57:09: 18000000 INFO @ Tue, 02 Aug 2022 15:57:20: 15000000 INFO @ Tue, 02 Aug 2022 15:57:23: 17000000 INFO @ Tue, 02 Aug 2022 15:57:26: 19000000 INFO @ Tue, 02 Aug 2022 15:57:35: 16000000 INFO @ Tue, 02 Aug 2022 15:57:37: 18000000 INFO @ Tue, 02 Aug 2022 15:57:42: 20000000 INFO @ Tue, 02 Aug 2022 15:57:50: 17000000 INFO @ Tue, 02 Aug 2022 15:57:52: 19000000 INFO @ Tue, 02 Aug 2022 15:57:58: 21000000 INFO @ Tue, 02 Aug 2022 15:58:05: 18000000 INFO @ Tue, 02 Aug 2022 15:58:07: 20000000 INFO @ Tue, 02 Aug 2022 15:58:13: 22000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:58:20: 19000000 INFO @ Tue, 02 Aug 2022 15:58:24: 21000000 INFO @ Tue, 02 Aug 2022 15:58:30: 23000000 INFO @ Tue, 02 Aug 2022 15:58:36: 20000000 INFO @ Tue, 02 Aug 2022 15:58:41: 22000000 INFO @ Tue, 02 Aug 2022 15:58:47: 24000000 INFO @ Tue, 02 Aug 2022 15:58:51: 21000000 INFO @ Tue, 02 Aug 2022 15:58:57: 23000000 INFO @ Tue, 02 Aug 2022 15:59:03: 25000000 INFO @ Tue, 02 Aug 2022 15:59:06: 22000000 INFO @ Tue, 02 Aug 2022 15:59:08: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:59:08: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:59:08: #1 total tags in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:59:08: #1 tags after filtering in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:59:08: #1 finished! INFO @ Tue, 02 Aug 2022 15:59:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:59:11: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 15:59:11: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:59:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:59:12: 24000000 INFO @ Tue, 02 Aug 2022 15:59:22: 23000000 INFO @ Tue, 02 Aug 2022 15:59:27: 25000000 INFO @ Tue, 02 Aug 2022 15:59:32: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:59:32: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:59:32: #1 total tags in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:59:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:59:33: #1 tags after filtering in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:59:33: #1 finished! INFO @ Tue, 02 Aug 2022 15:59:33: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:59:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:59:35: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 15:59:35: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:59:36: 24000000 INFO @ Tue, 02 Aug 2022 15:59:50: 25000000 INFO @ Tue, 02 Aug 2022 15:59:55: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 15:59:55: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 15:59:55: #1 total tags in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:59:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:59:55: #1 tags after filtering in treatment: 25322594 INFO @ Tue, 02 Aug 2022 15:59:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:59:55: #1 finished! INFO @ Tue, 02 Aug 2022 15:59:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:59:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:59:57: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 15:59:57: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:59:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206476/SRX15206476.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling