Job ID = 16439179 SRX = SRX15206459 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:42:49 prefetch.2.10.7: 1) Downloading 'SRR19139347'... 2022-08-02T05:42:49 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:43:03 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:43:04 prefetch.2.10.7: 'SRR19139347' is valid 2022-08-02T05:43:04 prefetch.2.10.7: 1) 'SRR19139347' was downloaded successfully 2022-08-02T05:43:04 prefetch.2.10.7: 'SRR19139347' has 0 unresolved dependencies Read 8384814 spots for SRR19139347/SRR19139347.sra Written 8384814 spots for SRR19139347/SRR19139347.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439279 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 8384814 reads; of these: 8384814 (100.00%) were unpaired; of these: 563509 (6.72%) aligned 0 times 6795669 (81.05%) aligned exactly 1 time 1025636 (12.23%) aligned >1 times 93.28% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 585090 / 7821305 = 0.0748 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:49:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:49:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:49:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:49:34: 1000000 INFO @ Tue, 02 Aug 2022 14:49:44: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:49:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:49:51: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:49:51: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:49:55: 3000000 INFO @ Tue, 02 Aug 2022 14:50:02: 1000000 INFO @ Tue, 02 Aug 2022 14:50:05: 4000000 INFO @ Tue, 02 Aug 2022 14:50:13: 2000000 INFO @ Tue, 02 Aug 2022 14:50:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:50:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:50:21: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:50:21: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:50:24: 3000000 INFO @ Tue, 02 Aug 2022 14:50:26: 6000000 INFO @ Tue, 02 Aug 2022 14:50:33: 1000000 INFO @ Tue, 02 Aug 2022 14:50:35: 4000000 INFO @ Tue, 02 Aug 2022 14:50:37: 7000000 INFO @ Tue, 02 Aug 2022 14:50:39: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:50:39: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:50:39: #1 total tags in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:50:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:50:39: #1 tags after filtering in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:50:39: #1 finished! INFO @ Tue, 02 Aug 2022 14:50:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:50:40: #2 number of paired peaks: 87 WARNING @ Tue, 02 Aug 2022 14:50:40: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:50:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:50:44: 2000000 INFO @ Tue, 02 Aug 2022 14:50:46: 5000000 INFO @ Tue, 02 Aug 2022 14:50:56: 6000000 INFO @ Tue, 02 Aug 2022 14:50:56: 3000000 INFO @ Tue, 02 Aug 2022 14:51:06: 7000000 INFO @ Tue, 02 Aug 2022 14:51:08: 4000000 INFO @ Tue, 02 Aug 2022 14:51:08: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:51:08: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:51:08: #1 total tags in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:51:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:51:08: #1 tags after filtering in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:51:08: #1 finished! INFO @ Tue, 02 Aug 2022 14:51:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:51:09: #2 number of paired peaks: 87 WARNING @ Tue, 02 Aug 2022 14:51:09: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:51:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:51:19: 5000000 INFO @ Tue, 02 Aug 2022 14:51:31: 6000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:51:43: 7000000 INFO @ Tue, 02 Aug 2022 14:51:45: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:51:45: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:51:45: #1 total tags in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:51:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:51:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:51:45: #1 tags after filtering in treatment: 7236215 INFO @ Tue, 02 Aug 2022 14:51:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:51:45: #1 finished! INFO @ Tue, 02 Aug 2022 14:51:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:51:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:51:46: #2 number of paired peaks: 87 WARNING @ Tue, 02 Aug 2022 14:51:46: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:51:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206459/SRX15206459.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling