Job ID = 16439168 SRX = SRX15206452 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T05:42:31 prefetch.2.10.7: 1) Downloading 'SRR19139354'... 2022-08-02T05:42:31 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T05:42:55 prefetch.2.10.7: HTTPS download succeed 2022-08-02T05:42:56 prefetch.2.10.7: 'SRR19139354' is valid 2022-08-02T05:42:56 prefetch.2.10.7: 1) 'SRR19139354' was downloaded successfully 2022-08-02T05:42:56 prefetch.2.10.7: 'SRR19139354' has 0 unresolved dependencies Read 12830987 spots for SRR19139354/SRR19139354.sra Written 12830987 spots for SRR19139354/SRR19139354.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439290 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:49 12830987 reads; of these: 12830987 (100.00%) were unpaired; of these: 218000 (1.70%) aligned 0 times 11323419 (88.25%) aligned exactly 1 time 1289568 (10.05%) aligned >1 times 98.30% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 790597 / 12612987 = 0.0627 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:53:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:53:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:53:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:53:52: 1000000 INFO @ Tue, 02 Aug 2022 14:54:00: 2000000 INFO @ Tue, 02 Aug 2022 14:54:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:54:14: 4000000 INFO @ Tue, 02 Aug 2022 14:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:54:15: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:54:15: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:54:21: 5000000 INFO @ Tue, 02 Aug 2022 14:54:22: 1000000 INFO @ Tue, 02 Aug 2022 14:54:28: 2000000 INFO @ Tue, 02 Aug 2022 14:54:29: 6000000 INFO @ Tue, 02 Aug 2022 14:54:35: 3000000 INFO @ Tue, 02 Aug 2022 14:54:36: 7000000 INFO @ Tue, 02 Aug 2022 14:54:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:54:43: 8000000 INFO @ Tue, 02 Aug 2022 14:54:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:54:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:54:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:54:48: 5000000 INFO @ Tue, 02 Aug 2022 14:54:51: 9000000 INFO @ Tue, 02 Aug 2022 14:54:53: 1000000 INFO @ Tue, 02 Aug 2022 14:54:56: 6000000 INFO @ Tue, 02 Aug 2022 14:55:00: 10000000 INFO @ Tue, 02 Aug 2022 14:55:01: 2000000 INFO @ Tue, 02 Aug 2022 14:55:04: 7000000 INFO @ Tue, 02 Aug 2022 14:55:08: 11000000 INFO @ Tue, 02 Aug 2022 14:55:08: 3000000 INFO @ Tue, 02 Aug 2022 14:55:11: 8000000 INFO @ Tue, 02 Aug 2022 14:55:14: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:55:14: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:55:14: #1 total tags in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:55:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:55:15: #1 tags after filtering in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:55:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:55:15: #1 finished! INFO @ Tue, 02 Aug 2022 14:55:15: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:55:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:55:15: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 14:55:15: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:55:16: 4000000 INFO @ Tue, 02 Aug 2022 14:55:18: 9000000 INFO @ Tue, 02 Aug 2022 14:55:24: 5000000 INFO @ Tue, 02 Aug 2022 14:55:26: 10000000 INFO @ Tue, 02 Aug 2022 14:55:31: 6000000 INFO @ Tue, 02 Aug 2022 14:55:33: 11000000 INFO @ Tue, 02 Aug 2022 14:55:39: 7000000 INFO @ Tue, 02 Aug 2022 14:55:39: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:55:39: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:55:39: #1 total tags in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:55:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:55:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:55:39: #1 tags after filtering in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:55:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:55:39: #1 finished! INFO @ Tue, 02 Aug 2022 14:55:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:55:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:55:40: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 14:55:40: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:55:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:55:45: 8000000 INFO @ Tue, 02 Aug 2022 14:55:51: 9000000 INFO @ Tue, 02 Aug 2022 14:55:57: 10000000 INFO @ Tue, 02 Aug 2022 14:56:03: 11000000 INFO @ Tue, 02 Aug 2022 14:56:08: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:56:08: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:56:08: #1 total tags in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:56:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:56:08: #1 tags after filtering in treatment: 11822390 INFO @ Tue, 02 Aug 2022 14:56:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:56:08: #1 finished! INFO @ Tue, 02 Aug 2022 14:56:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:56:09: #2 number of paired peaks: 82 WARNING @ Tue, 02 Aug 2022 14:56:09: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:56:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15206452/SRX15206452.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。