Job ID = 16437424 SRX = SRX15046048 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20486735 spots for SRR18970067/SRR18970067.sra Written 20486735 spots for SRR18970067/SRR18970067.sra Read 5210147 spots for SRR18970068/SRR18970068.sra Written 5210147 spots for SRR18970068/SRR18970068.sra fastq に変換しました。 bowtie でマッピング中... Your job 16437478 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:42 25696882 reads; of these: 25696882 (100.00%) were unpaired; of these: 1269524 (4.94%) aligned 0 times 19125828 (74.43%) aligned exactly 1 time 5301530 (20.63%) aligned >1 times 95.06% overall alignment rate Time searching: 00:07:42 Overall time: 00:07:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3022621 / 24427358 = 0.1237 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:41:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:41:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:41:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:41:16: 1000000 INFO @ Tue, 02 Aug 2022 12:41:23: 2000000 INFO @ Tue, 02 Aug 2022 12:41:29: 3000000 INFO @ Tue, 02 Aug 2022 12:41:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:41:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:41:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:41:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:41:43: 5000000 INFO @ Tue, 02 Aug 2022 12:41:47: 1000000 INFO @ Tue, 02 Aug 2022 12:41:50: 6000000 INFO @ Tue, 02 Aug 2022 12:41:55: 2000000 INFO @ Tue, 02 Aug 2022 12:41:58: 7000000 INFO @ Tue, 02 Aug 2022 12:42:02: 3000000 INFO @ Tue, 02 Aug 2022 12:42:05: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:42:09: 4000000 INFO @ Tue, 02 Aug 2022 12:42:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:42:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:42:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:42:13: 9000000 INFO @ Tue, 02 Aug 2022 12:42:17: 5000000 INFO @ Tue, 02 Aug 2022 12:42:18: 1000000 INFO @ Tue, 02 Aug 2022 12:42:21: 10000000 INFO @ Tue, 02 Aug 2022 12:42:24: 6000000 INFO @ Tue, 02 Aug 2022 12:42:25: 2000000 INFO @ Tue, 02 Aug 2022 12:42:29: 11000000 INFO @ Tue, 02 Aug 2022 12:42:32: 7000000 INFO @ Tue, 02 Aug 2022 12:42:33: 3000000 INFO @ Tue, 02 Aug 2022 12:42:37: 12000000 INFO @ Tue, 02 Aug 2022 12:42:39: 8000000 INFO @ Tue, 02 Aug 2022 12:42:41: 4000000 INFO @ Tue, 02 Aug 2022 12:42:44: 13000000 INFO @ Tue, 02 Aug 2022 12:42:47: 9000000 INFO @ Tue, 02 Aug 2022 12:42:49: 5000000 INFO @ Tue, 02 Aug 2022 12:42:52: 14000000 INFO @ Tue, 02 Aug 2022 12:42:55: 10000000 INFO @ Tue, 02 Aug 2022 12:42:57: 6000000 INFO @ Tue, 02 Aug 2022 12:43:00: 15000000 INFO @ Tue, 02 Aug 2022 12:43:02: 11000000 INFO @ Tue, 02 Aug 2022 12:43:05: 7000000 INFO @ Tue, 02 Aug 2022 12:43:08: 16000000 INFO @ Tue, 02 Aug 2022 12:43:09: 12000000 INFO @ Tue, 02 Aug 2022 12:43:12: 8000000 INFO @ Tue, 02 Aug 2022 12:43:16: 17000000 INFO @ Tue, 02 Aug 2022 12:43:17: 13000000 INFO @ Tue, 02 Aug 2022 12:43:20: 9000000 INFO @ Tue, 02 Aug 2022 12:43:24: 18000000 INFO @ Tue, 02 Aug 2022 12:43:25: 14000000 INFO @ Tue, 02 Aug 2022 12:43:27: 10000000 INFO @ Tue, 02 Aug 2022 12:43:31: 19000000 INFO @ Tue, 02 Aug 2022 12:43:32: 15000000 INFO @ Tue, 02 Aug 2022 12:43:35: 11000000 INFO @ Tue, 02 Aug 2022 12:43:39: 20000000 INFO @ Tue, 02 Aug 2022 12:43:40: 16000000 INFO @ Tue, 02 Aug 2022 12:43:42: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:43:47: 21000000 INFO @ Tue, 02 Aug 2022 12:43:48: 17000000 INFO @ Tue, 02 Aug 2022 12:43:50: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:43:50: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:43:50: #1 total tags in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:43:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:43:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:43:50: 13000000 INFO @ Tue, 02 Aug 2022 12:43:50: #1 tags after filtering in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:43:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:43:50: #1 finished! INFO @ Tue, 02 Aug 2022 12:43:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:43:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:43:52: #2 number of paired peaks: 17 WARNING @ Tue, 02 Aug 2022 12:43:52: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:43:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:43:56: 18000000 INFO @ Tue, 02 Aug 2022 12:43:57: 14000000 INFO @ Tue, 02 Aug 2022 12:44:03: 19000000 INFO @ Tue, 02 Aug 2022 12:44:04: 15000000 INFO @ Tue, 02 Aug 2022 12:44:10: 20000000 INFO @ Tue, 02 Aug 2022 12:44:11: 16000000 INFO @ Tue, 02 Aug 2022 12:44:18: 21000000 INFO @ Tue, 02 Aug 2022 12:44:19: 17000000 INFO @ Tue, 02 Aug 2022 12:44:21: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:44:21: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:44:21: #1 total tags in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:44:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:44:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:44:21: #1 tags after filtering in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:44:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:44:21: #1 finished! INFO @ Tue, 02 Aug 2022 12:44:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:44:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:44:23: #2 number of paired peaks: 17 WARNING @ Tue, 02 Aug 2022 12:44:23: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:44:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:44:25: 18000000 INFO @ Tue, 02 Aug 2022 12:44:31: 19000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:44:38: 20000000 INFO @ Tue, 02 Aug 2022 12:44:44: 21000000 INFO @ Tue, 02 Aug 2022 12:44:46: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:44:46: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:44:46: #1 total tags in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:44:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:44:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:44:47: #1 tags after filtering in treatment: 21404737 INFO @ Tue, 02 Aug 2022 12:44:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:44:47: #1 finished! INFO @ Tue, 02 Aug 2022 12:44:47: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:44:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:44:48: #2 number of paired peaks: 17 WARNING @ Tue, 02 Aug 2022 12:44:48: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 12:44:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15046048/SRX15046048.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling