Job ID = 16439749 SRX = SRX14894238 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T07:05:33 prefetch.2.10.7: 1) Downloading 'SRR18795783'... 2022-08-02T07:05:33 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:07:01 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:07:01 prefetch.2.10.7: 1) 'SRR18795783' was downloaded successfully 2022-08-02T07:07:01 prefetch.2.10.7: 'SRR18795783' has 0 unresolved dependencies Read 68719034 spots for SRR18795783/SRR18795783.sra Written 68719034 spots for SRR18795783/SRR18795783.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440025 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:36 68719034 reads; of these: 68719034 (100.00%) were unpaired; of these: 6156766 (8.96%) aligned 0 times 45674910 (66.47%) aligned exactly 1 time 16887358 (24.57%) aligned >1 times 91.04% overall alignment rate Time searching: 00:24:36 Overall time: 00:24:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 18039526 / 62562268 = 0.2883 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:48:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:48:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:48:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:48:13: 1000000 INFO @ Tue, 02 Aug 2022 16:48:19: 2000000 INFO @ Tue, 02 Aug 2022 16:48:25: 3000000 INFO @ Tue, 02 Aug 2022 16:48:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:48:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:48:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:48:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:48:38: 5000000 INFO @ Tue, 02 Aug 2022 16:48:42: 1000000 INFO @ Tue, 02 Aug 2022 16:48:44: 6000000 INFO @ Tue, 02 Aug 2022 16:48:49: 2000000 INFO @ Tue, 02 Aug 2022 16:48:51: 7000000 INFO @ Tue, 02 Aug 2022 16:48:55: 3000000 INFO @ Tue, 02 Aug 2022 16:48:57: 8000000 INFO @ Tue, 02 Aug 2022 16:49:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:49:04: 9000000 INFO @ Tue, 02 Aug 2022 16:49:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:49:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:49:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:49:08: 5000000 INFO @ Tue, 02 Aug 2022 16:49:11: 10000000 INFO @ Tue, 02 Aug 2022 16:49:11: 1000000 INFO @ Tue, 02 Aug 2022 16:49:15: 6000000 INFO @ Tue, 02 Aug 2022 16:49:17: 11000000 INFO @ Tue, 02 Aug 2022 16:49:18: 2000000 INFO @ Tue, 02 Aug 2022 16:49:22: 7000000 INFO @ Tue, 02 Aug 2022 16:49:25: 12000000 INFO @ Tue, 02 Aug 2022 16:49:25: 3000000 INFO @ Tue, 02 Aug 2022 16:49:29: 8000000 INFO @ Tue, 02 Aug 2022 16:49:31: 13000000 INFO @ Tue, 02 Aug 2022 16:49:32: 4000000 INFO @ Tue, 02 Aug 2022 16:49:35: 9000000 INFO @ Tue, 02 Aug 2022 16:49:38: 14000000 INFO @ Tue, 02 Aug 2022 16:49:38: 5000000 INFO @ Tue, 02 Aug 2022 16:49:42: 10000000 INFO @ Tue, 02 Aug 2022 16:49:44: 15000000 INFO @ Tue, 02 Aug 2022 16:49:45: 6000000 INFO @ Tue, 02 Aug 2022 16:49:49: 11000000 INFO @ Tue, 02 Aug 2022 16:49:51: 16000000 INFO @ Tue, 02 Aug 2022 16:49:52: 7000000 INFO @ Tue, 02 Aug 2022 16:49:55: 12000000 INFO @ Tue, 02 Aug 2022 16:49:57: 17000000 INFO @ Tue, 02 Aug 2022 16:49:58: 8000000 INFO @ Tue, 02 Aug 2022 16:50:02: 13000000 INFO @ Tue, 02 Aug 2022 16:50:04: 18000000 INFO @ Tue, 02 Aug 2022 16:50:05: 9000000 INFO @ Tue, 02 Aug 2022 16:50:09: 14000000 INFO @ Tue, 02 Aug 2022 16:50:10: 19000000 INFO @ Tue, 02 Aug 2022 16:50:11: 10000000 INFO @ Tue, 02 Aug 2022 16:50:15: 15000000 INFO @ Tue, 02 Aug 2022 16:50:17: 20000000 INFO @ Tue, 02 Aug 2022 16:50:18: 11000000 INFO @ Tue, 02 Aug 2022 16:50:22: 16000000 INFO @ Tue, 02 Aug 2022 16:50:24: 21000000 INFO @ Tue, 02 Aug 2022 16:50:25: 12000000 INFO @ Tue, 02 Aug 2022 16:50:28: 17000000 INFO @ Tue, 02 Aug 2022 16:50:31: 22000000 INFO @ Tue, 02 Aug 2022 16:50:31: 13000000 INFO @ Tue, 02 Aug 2022 16:50:35: 18000000 INFO @ Tue, 02 Aug 2022 16:50:37: 23000000 INFO @ Tue, 02 Aug 2022 16:50:38: 14000000 INFO @ Tue, 02 Aug 2022 16:50:42: 19000000 INFO @ Tue, 02 Aug 2022 16:50:44: 24000000 INFO @ Tue, 02 Aug 2022 16:50:44: 15000000 INFO @ Tue, 02 Aug 2022 16:50:48: 20000000 INFO @ Tue, 02 Aug 2022 16:50:50: 25000000 INFO @ Tue, 02 Aug 2022 16:50:51: 16000000 INFO @ Tue, 02 Aug 2022 16:50:55: 21000000 INFO @ Tue, 02 Aug 2022 16:50:56: 26000000 INFO @ Tue, 02 Aug 2022 16:50:57: 17000000 INFO @ Tue, 02 Aug 2022 16:51:02: 22000000 INFO @ Tue, 02 Aug 2022 16:51:03: 27000000 INFO @ Tue, 02 Aug 2022 16:51:04: 18000000 INFO @ Tue, 02 Aug 2022 16:51:09: 23000000 INFO @ Tue, 02 Aug 2022 16:51:09: 28000000 INFO @ Tue, 02 Aug 2022 16:51:10: 19000000 INFO @ Tue, 02 Aug 2022 16:51:16: 24000000 INFO @ Tue, 02 Aug 2022 16:51:16: 29000000 INFO @ Tue, 02 Aug 2022 16:51:17: 20000000 INFO @ Tue, 02 Aug 2022 16:51:23: 25000000 INFO @ Tue, 02 Aug 2022 16:51:23: 30000000 INFO @ Tue, 02 Aug 2022 16:51:24: 21000000 INFO @ Tue, 02 Aug 2022 16:51:29: 26000000 INFO @ Tue, 02 Aug 2022 16:51:29: 31000000 INFO @ Tue, 02 Aug 2022 16:51:30: 22000000 INFO @ Tue, 02 Aug 2022 16:51:35: 27000000 INFO @ Tue, 02 Aug 2022 16:51:36: 32000000 INFO @ Tue, 02 Aug 2022 16:51:37: 23000000 INFO @ Tue, 02 Aug 2022 16:51:42: 28000000 INFO @ Tue, 02 Aug 2022 16:51:43: 33000000 INFO @ Tue, 02 Aug 2022 16:51:43: 24000000 INFO @ Tue, 02 Aug 2022 16:51:48: 29000000 INFO @ Tue, 02 Aug 2022 16:51:49: 34000000 INFO @ Tue, 02 Aug 2022 16:51:50: 25000000 INFO @ Tue, 02 Aug 2022 16:51:54: 30000000 INFO @ Tue, 02 Aug 2022 16:51:56: 35000000 INFO @ Tue, 02 Aug 2022 16:51:56: 26000000 INFO @ Tue, 02 Aug 2022 16:52:01: 31000000 INFO @ Tue, 02 Aug 2022 16:52:02: 36000000 INFO @ Tue, 02 Aug 2022 16:52:03: 27000000 INFO @ Tue, 02 Aug 2022 16:52:07: 32000000 INFO @ Tue, 02 Aug 2022 16:52:09: 37000000 INFO @ Tue, 02 Aug 2022 16:52:09: 28000000 INFO @ Tue, 02 Aug 2022 16:52:14: 33000000 INFO @ Tue, 02 Aug 2022 16:52:15: 38000000 INFO @ Tue, 02 Aug 2022 16:52:16: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:52:20: 34000000 INFO @ Tue, 02 Aug 2022 16:52:22: 39000000 INFO @ Tue, 02 Aug 2022 16:52:22: 30000000 INFO @ Tue, 02 Aug 2022 16:52:27: 35000000 INFO @ Tue, 02 Aug 2022 16:52:28: 40000000 INFO @ Tue, 02 Aug 2022 16:52:29: 31000000 INFO @ Tue, 02 Aug 2022 16:52:33: 36000000 INFO @ Tue, 02 Aug 2022 16:52:35: 41000000 INFO @ Tue, 02 Aug 2022 16:52:35: 32000000 INFO @ Tue, 02 Aug 2022 16:52:39: 37000000 INFO @ Tue, 02 Aug 2022 16:52:42: 42000000 INFO @ Tue, 02 Aug 2022 16:52:42: 33000000 INFO @ Tue, 02 Aug 2022 16:52:46: 38000000 INFO @ Tue, 02 Aug 2022 16:52:48: 43000000 INFO @ Tue, 02 Aug 2022 16:52:49: 34000000 INFO @ Tue, 02 Aug 2022 16:52:52: 39000000 INFO @ Tue, 02 Aug 2022 16:52:55: 44000000 INFO @ Tue, 02 Aug 2022 16:52:56: 35000000 INFO @ Tue, 02 Aug 2022 16:52:59: 40000000 INFO @ Tue, 02 Aug 2022 16:52:59: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:52:59: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:52:59: #1 total tags in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:52:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:52:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:52:59: #1 tags after filtering in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:52:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:52:59: #1 finished! INFO @ Tue, 02 Aug 2022 16:52:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:52:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:53:02: 36000000 INFO @ Tue, 02 Aug 2022 16:53:03: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:53:03: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:53:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:53:05: 41000000 INFO @ Tue, 02 Aug 2022 16:53:08: 37000000 INFO @ Tue, 02 Aug 2022 16:53:11: 42000000 INFO @ Tue, 02 Aug 2022 16:53:15: 38000000 INFO @ Tue, 02 Aug 2022 16:53:18: 43000000 INFO @ Tue, 02 Aug 2022 16:53:21: 39000000 INFO @ Tue, 02 Aug 2022 16:53:24: 44000000 INFO @ Tue, 02 Aug 2022 16:53:28: 40000000 INFO @ Tue, 02 Aug 2022 16:53:28: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:53:28: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:53:28: #1 total tags in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:53:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:53:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:53:29: #1 tags after filtering in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:53:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:53:29: #1 finished! INFO @ Tue, 02 Aug 2022 16:53:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:53:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:53:32: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:53:32: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:53:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:53:34: 41000000 INFO @ Tue, 02 Aug 2022 16:53:40: 42000000 INFO @ Tue, 02 Aug 2022 16:53:47: 43000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:53:53: 44000000 INFO @ Tue, 02 Aug 2022 16:53:56: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:53:56: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:53:56: #1 total tags in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:53:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:53:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:53:57: #1 tags after filtering in treatment: 44522742 INFO @ Tue, 02 Aug 2022 16:53:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:53:57: #1 finished! INFO @ Tue, 02 Aug 2022 16:53:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:53:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:54:00: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 16:54:00: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:54:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14894238/SRX14894238.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling