Job ID = 16439372 SRX = SRX14232182 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20255613 spots for SRR18080636/SRR18080636.sra Written 20255613 spots for SRR18080636/SRR18080636.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440008 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:28:18 20255613 reads; of these: 20255613 (100.00%) were paired; of these: 6111807 (30.17%) aligned concordantly 0 times 10977308 (54.19%) aligned concordantly exactly 1 time 3166498 (15.63%) aligned concordantly >1 times ---- 6111807 pairs aligned concordantly 0 times; of these: 2632844 (43.08%) aligned discordantly 1 time ---- 3478963 pairs aligned 0 times concordantly or discordantly; of these: 6957926 mates make up the pairs; of these: 5118224 (73.56%) aligned 0 times 463260 (6.66%) aligned exactly 1 time 1376442 (19.78%) aligned >1 times 87.37% overall alignment rate Time searching: 01:28:19 Overall time: 01:28:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3034054 / 16477351 = 0.1841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:54:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:54:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:54:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:54:37: 1000000 INFO @ Tue, 02 Aug 2022 16:54:49: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:54:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:54:53: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:54:53: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:01: 3000000 INFO @ Tue, 02 Aug 2022 16:55:04: 1000000 INFO @ Tue, 02 Aug 2022 16:55:13: 4000000 INFO @ Tue, 02 Aug 2022 16:55:14: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:23: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:23: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:24: 3000000 INFO @ Tue, 02 Aug 2022 16:55:25: 5000000 INFO @ Tue, 02 Aug 2022 16:55:34: 4000000 INFO @ Tue, 02 Aug 2022 16:55:36: 1000000 INFO @ Tue, 02 Aug 2022 16:55:36: 6000000 INFO @ Tue, 02 Aug 2022 16:55:43: 5000000 INFO @ Tue, 02 Aug 2022 16:55:47: 2000000 INFO @ Tue, 02 Aug 2022 16:55:48: 7000000 INFO @ Tue, 02 Aug 2022 16:55:54: 6000000 INFO @ Tue, 02 Aug 2022 16:55:58: 3000000 INFO @ Tue, 02 Aug 2022 16:56:00: 8000000 INFO @ Tue, 02 Aug 2022 16:56:04: 7000000 INFO @ Tue, 02 Aug 2022 16:56:08: 4000000 INFO @ Tue, 02 Aug 2022 16:56:10: 9000000 INFO @ Tue, 02 Aug 2022 16:56:14: 8000000 INFO @ Tue, 02 Aug 2022 16:56:19: 5000000 INFO @ Tue, 02 Aug 2022 16:56:21: 10000000 INFO @ Tue, 02 Aug 2022 16:56:25: 9000000 INFO @ Tue, 02 Aug 2022 16:56:30: 6000000 INFO @ Tue, 02 Aug 2022 16:56:31: 11000000 INFO @ Tue, 02 Aug 2022 16:56:36: 10000000 INFO @ Tue, 02 Aug 2022 16:56:40: 7000000 INFO @ Tue, 02 Aug 2022 16:56:41: 12000000 INFO @ Tue, 02 Aug 2022 16:56:47: 11000000 INFO @ Tue, 02 Aug 2022 16:56:51: 8000000 INFO @ Tue, 02 Aug 2022 16:56:51: 13000000 INFO @ Tue, 02 Aug 2022 16:56:58: 12000000 INFO @ Tue, 02 Aug 2022 16:57:01: 9000000 INFO @ Tue, 02 Aug 2022 16:57:02: 14000000 INFO @ Tue, 02 Aug 2022 16:57:08: 13000000 INFO @ Tue, 02 Aug 2022 16:57:12: 15000000 INFO @ Tue, 02 Aug 2022 16:57:12: 10000000 INFO @ Tue, 02 Aug 2022 16:57:20: 14000000 INFO @ Tue, 02 Aug 2022 16:57:22: 16000000 INFO @ Tue, 02 Aug 2022 16:57:23: 11000000 INFO @ Tue, 02 Aug 2022 16:57:32: 15000000 INFO @ Tue, 02 Aug 2022 16:57:34: 17000000 INFO @ Tue, 02 Aug 2022 16:57:34: 12000000 INFO @ Tue, 02 Aug 2022 16:57:43: 16000000 INFO @ Tue, 02 Aug 2022 16:57:44: 18000000 INFO @ Tue, 02 Aug 2022 16:57:45: 13000000 INFO @ Tue, 02 Aug 2022 16:57:54: 17000000 INFO @ Tue, 02 Aug 2022 16:57:55: 19000000 INFO @ Tue, 02 Aug 2022 16:57:57: 14000000 INFO @ Tue, 02 Aug 2022 16:58:05: 18000000 INFO @ Tue, 02 Aug 2022 16:58:06: 20000000 INFO @ Tue, 02 Aug 2022 16:58:10: 15000000 INFO @ Tue, 02 Aug 2022 16:58:16: 21000000 INFO @ Tue, 02 Aug 2022 16:58:16: 19000000 INFO @ Tue, 02 Aug 2022 16:58:21: 16000000 INFO @ Tue, 02 Aug 2022 16:58:27: 22000000 INFO @ Tue, 02 Aug 2022 16:58:27: 20000000 INFO @ Tue, 02 Aug 2022 16:58:33: 17000000 INFO @ Tue, 02 Aug 2022 16:58:37: 23000000 INFO @ Tue, 02 Aug 2022 16:58:39: 21000000 INFO @ Tue, 02 Aug 2022 16:58:44: 18000000 INFO @ Tue, 02 Aug 2022 16:58:47: 24000000 INFO @ Tue, 02 Aug 2022 16:58:50: 22000000 INFO @ Tue, 02 Aug 2022 16:58:55: 19000000 INFO @ Tue, 02 Aug 2022 16:58:58: 25000000 INFO @ Tue, 02 Aug 2022 16:59:01: 23000000 INFO @ Tue, 02 Aug 2022 16:59:06: 20000000 INFO @ Tue, 02 Aug 2022 16:59:08: 26000000 INFO @ Tue, 02 Aug 2022 16:59:11: 24000000 INFO @ Tue, 02 Aug 2022 16:59:17: 21000000 INFO @ Tue, 02 Aug 2022 16:59:19: 27000000 INFO @ Tue, 02 Aug 2022 16:59:22: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:59:28: 22000000 INFO @ Tue, 02 Aug 2022 16:59:30: 28000000 INFO @ Tue, 02 Aug 2022 16:59:33: 26000000 INFO @ Tue, 02 Aug 2022 16:59:39: 23000000 INFO @ Tue, 02 Aug 2022 16:59:42: 29000000 INFO @ Tue, 02 Aug 2022 16:59:43: 27000000 INFO @ Tue, 02 Aug 2022 16:59:46: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 16:59:46: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 16:59:46: #1 total tags in treatment: 11549603 INFO @ Tue, 02 Aug 2022 16:59:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:59:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:59:46: #1 tags after filtering in treatment: 10762735 INFO @ Tue, 02 Aug 2022 16:59:46: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 16:59:46: #1 finished! INFO @ Tue, 02 Aug 2022 16:59:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:59:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:59:47: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 16:59:47: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:59:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:59:49: 24000000 INFO @ Tue, 02 Aug 2022 16:59:53: 28000000 INFO @ Tue, 02 Aug 2022 17:00:00: 25000000 INFO @ Tue, 02 Aug 2022 17:00:04: 29000000 INFO @ Tue, 02 Aug 2022 17:00:08: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 17:00:08: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 17:00:08: #1 total tags in treatment: 11549603 INFO @ Tue, 02 Aug 2022 17:00:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:00:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:00:08: #1 tags after filtering in treatment: 10762735 INFO @ Tue, 02 Aug 2022 17:00:08: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 17:00:08: #1 finished! INFO @ Tue, 02 Aug 2022 17:00:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:00:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:00:09: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 17:00:09: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:00:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:00:11: 26000000 INFO @ Tue, 02 Aug 2022 17:00:21: 27000000 INFO @ Tue, 02 Aug 2022 17:00:31: 28000000 INFO @ Tue, 02 Aug 2022 17:00:41: 29000000 INFO @ Tue, 02 Aug 2022 17:00:45: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 17:00:45: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 17:00:45: #1 total tags in treatment: 11549603 INFO @ Tue, 02 Aug 2022 17:00:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:00:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:00:45: #1 tags after filtering in treatment: 10762735 INFO @ Tue, 02 Aug 2022 17:00:45: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 17:00:45: #1 finished! INFO @ Tue, 02 Aug 2022 17:00:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:00:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:00:46: #2 number of paired peaks: 80 WARNING @ Tue, 02 Aug 2022 17:00:46: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:00:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX14232182/SRX14232182.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。