Job ID = 16439637 SRX = SRX13572055 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19205968 spots for SRR17399990/SRR17399990.sra Written 19205968 spots for SRR17399990/SRR17399990.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439686 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:29 19205968 reads; of these: 19205968 (100.00%) were unpaired; of these: 874130 (4.55%) aligned 0 times 15575801 (81.10%) aligned exactly 1 time 2756037 (14.35%) aligned >1 times 95.45% overall alignment rate Time searching: 00:05:29 Overall time: 00:05:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1544987 / 18331838 = 0.0843 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:59:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:59:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:59:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:59:08: 1000000 INFO @ Tue, 02 Aug 2022 15:59:16: 2000000 INFO @ Tue, 02 Aug 2022 15:59:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:59:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:59:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:59:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:59:32: 4000000 INFO @ Tue, 02 Aug 2022 15:59:37: 1000000 INFO @ Tue, 02 Aug 2022 15:59:40: 5000000 INFO @ Tue, 02 Aug 2022 15:59:45: 2000000 INFO @ Tue, 02 Aug 2022 15:59:49: 6000000 INFO @ Tue, 02 Aug 2022 15:59:53: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:59:58: 7000000 INFO @ Tue, 02 Aug 2022 15:59:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:59:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:59:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:00:01: 4000000 INFO @ Tue, 02 Aug 2022 16:00:07: 8000000 INFO @ Tue, 02 Aug 2022 16:00:08: 1000000 INFO @ Tue, 02 Aug 2022 16:00:10: 5000000 INFO @ Tue, 02 Aug 2022 16:00:16: 2000000 INFO @ Tue, 02 Aug 2022 16:00:16: 9000000 INFO @ Tue, 02 Aug 2022 16:00:19: 6000000 INFO @ Tue, 02 Aug 2022 16:00:25: 3000000 INFO @ Tue, 02 Aug 2022 16:00:25: 10000000 INFO @ Tue, 02 Aug 2022 16:00:27: 7000000 INFO @ Tue, 02 Aug 2022 16:00:33: 4000000 INFO @ Tue, 02 Aug 2022 16:00:36: 8000000 INFO @ Tue, 02 Aug 2022 16:00:36: 11000000 INFO @ Tue, 02 Aug 2022 16:00:41: 5000000 INFO @ Tue, 02 Aug 2022 16:00:45: 9000000 INFO @ Tue, 02 Aug 2022 16:00:46: 12000000 INFO @ Tue, 02 Aug 2022 16:00:50: 6000000 INFO @ Tue, 02 Aug 2022 16:00:54: 10000000 INFO @ Tue, 02 Aug 2022 16:00:55: 13000000 INFO @ Tue, 02 Aug 2022 16:00:58: 7000000 INFO @ Tue, 02 Aug 2022 16:01:02: 11000000 INFO @ Tue, 02 Aug 2022 16:01:04: 14000000 INFO @ Tue, 02 Aug 2022 16:01:06: 8000000 INFO @ Tue, 02 Aug 2022 16:01:11: 12000000 INFO @ Tue, 02 Aug 2022 16:01:13: 15000000 INFO @ Tue, 02 Aug 2022 16:01:15: 9000000 INFO @ Tue, 02 Aug 2022 16:01:19: 13000000 INFO @ Tue, 02 Aug 2022 16:01:22: 16000000 INFO @ Tue, 02 Aug 2022 16:01:23: 10000000 INFO @ Tue, 02 Aug 2022 16:01:28: 14000000 INFO @ Tue, 02 Aug 2022 16:01:29: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:01:29: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:01:29: #1 total tags in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:01:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:01:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:01:29: #1 tags after filtering in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:01:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:01:29: #1 finished! INFO @ Tue, 02 Aug 2022 16:01:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:01:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:01:30: #2 number of paired peaks: 43 WARNING @ Tue, 02 Aug 2022 16:01:30: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:01:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:01:32: 11000000 INFO @ Tue, 02 Aug 2022 16:01:36: 15000000 INFO @ Tue, 02 Aug 2022 16:01:40: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:01:44: 16000000 INFO @ Tue, 02 Aug 2022 16:01:49: 13000000 INFO @ Tue, 02 Aug 2022 16:01:51: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:01:51: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:01:51: #1 total tags in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:01:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:01:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:01:51: #1 tags after filtering in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:01:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:01:51: #1 finished! INFO @ Tue, 02 Aug 2022 16:01:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:01:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:01:52: #2 number of paired peaks: 43 WARNING @ Tue, 02 Aug 2022 16:01:52: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:01:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:01:58: 14000000 INFO @ Tue, 02 Aug 2022 16:02:06: 15000000 INFO @ Tue, 02 Aug 2022 16:02:15: 16000000 INFO @ Tue, 02 Aug 2022 16:02:22: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:02:22: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:02:22: #1 total tags in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:02:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:02:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:02:23: #1 tags after filtering in treatment: 16786851 INFO @ Tue, 02 Aug 2022 16:02:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:02:23: #1 finished! INFO @ Tue, 02 Aug 2022 16:02:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:02:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:02:24: #2 number of paired peaks: 43 WARNING @ Tue, 02 Aug 2022 16:02:24: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:02:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572055/SRX13572055.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。