Job ID = 16439635 SRX = SRX13572053 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 59463883 spots for SRR17399995/SRR17399995.sra Written 59463883 spots for SRR17399995/SRR17399995.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439760 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:13 59463883 reads; of these: 59463883 (100.00%) were unpaired; of these: 2291935 (3.85%) aligned 0 times 46906836 (78.88%) aligned exactly 1 time 10265112 (17.26%) aligned >1 times 96.15% overall alignment rate Time searching: 00:16:13 Overall time: 00:16:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 6964118 / 57171948 = 0.1218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:19:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:19:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:19:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:19:09: 1000000 INFO @ Tue, 02 Aug 2022 16:19:15: 2000000 INFO @ Tue, 02 Aug 2022 16:19:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:19:29: 4000000 INFO @ Tue, 02 Aug 2022 16:19:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:19:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:19:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:19:36: 5000000 INFO @ Tue, 02 Aug 2022 16:19:38: 1000000 INFO @ Tue, 02 Aug 2022 16:19:44: 6000000 INFO @ Tue, 02 Aug 2022 16:19:46: 2000000 INFO @ Tue, 02 Aug 2022 16:19:51: 7000000 INFO @ Tue, 02 Aug 2022 16:19:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:19:59: 8000000 INFO @ Tue, 02 Aug 2022 16:20:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:20:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:20:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:20:02: 4000000 INFO @ Tue, 02 Aug 2022 16:20:07: 9000000 INFO @ Tue, 02 Aug 2022 16:20:08: 1000000 INFO @ Tue, 02 Aug 2022 16:20:10: 5000000 INFO @ Tue, 02 Aug 2022 16:20:15: 10000000 INFO @ Tue, 02 Aug 2022 16:20:16: 2000000 INFO @ Tue, 02 Aug 2022 16:20:19: 6000000 INFO @ Tue, 02 Aug 2022 16:20:23: 11000000 INFO @ Tue, 02 Aug 2022 16:20:25: 3000000 INFO @ Tue, 02 Aug 2022 16:20:27: 7000000 INFO @ Tue, 02 Aug 2022 16:20:33: 12000000 INFO @ Tue, 02 Aug 2022 16:20:35: 4000000 INFO @ Tue, 02 Aug 2022 16:20:37: 8000000 INFO @ Tue, 02 Aug 2022 16:20:41: 13000000 INFO @ Tue, 02 Aug 2022 16:20:44: 5000000 INFO @ Tue, 02 Aug 2022 16:20:45: 9000000 INFO @ Tue, 02 Aug 2022 16:20:50: 14000000 INFO @ Tue, 02 Aug 2022 16:20:52: 10000000 INFO @ Tue, 02 Aug 2022 16:20:53: 6000000 INFO @ Tue, 02 Aug 2022 16:20:58: 15000000 INFO @ Tue, 02 Aug 2022 16:21:00: 11000000 INFO @ Tue, 02 Aug 2022 16:21:01: 7000000 INFO @ Tue, 02 Aug 2022 16:21:07: 16000000 INFO @ Tue, 02 Aug 2022 16:21:07: 12000000 INFO @ Tue, 02 Aug 2022 16:21:10: 8000000 INFO @ Tue, 02 Aug 2022 16:21:15: 17000000 INFO @ Tue, 02 Aug 2022 16:21:15: 13000000 INFO @ Tue, 02 Aug 2022 16:21:19: 9000000 INFO @ Tue, 02 Aug 2022 16:21:23: 18000000 INFO @ Tue, 02 Aug 2022 16:21:23: 14000000 INFO @ Tue, 02 Aug 2022 16:21:27: 10000000 INFO @ Tue, 02 Aug 2022 16:21:31: 19000000 INFO @ Tue, 02 Aug 2022 16:21:32: 15000000 INFO @ Tue, 02 Aug 2022 16:21:35: 11000000 INFO @ Tue, 02 Aug 2022 16:21:40: 20000000 INFO @ Tue, 02 Aug 2022 16:21:40: 16000000 INFO @ Tue, 02 Aug 2022 16:21:44: 12000000 INFO @ Tue, 02 Aug 2022 16:21:48: 17000000 INFO @ Tue, 02 Aug 2022 16:21:48: 21000000 INFO @ Tue, 02 Aug 2022 16:21:51: 13000000 INFO @ Tue, 02 Aug 2022 16:21:55: 18000000 INFO @ Tue, 02 Aug 2022 16:21:57: 22000000 INFO @ Tue, 02 Aug 2022 16:21:59: 14000000 INFO @ Tue, 02 Aug 2022 16:22:03: 19000000 INFO @ Tue, 02 Aug 2022 16:22:05: 23000000 INFO @ Tue, 02 Aug 2022 16:22:07: 15000000 INFO @ Tue, 02 Aug 2022 16:22:10: 20000000 INFO @ Tue, 02 Aug 2022 16:22:14: 24000000 INFO @ Tue, 02 Aug 2022 16:22:16: 16000000 INFO @ Tue, 02 Aug 2022 16:22:18: 21000000 INFO @ Tue, 02 Aug 2022 16:22:22: 25000000 INFO @ Tue, 02 Aug 2022 16:22:24: 17000000 INFO @ Tue, 02 Aug 2022 16:22:26: 22000000 INFO @ Tue, 02 Aug 2022 16:22:31: 26000000 INFO @ Tue, 02 Aug 2022 16:22:33: 18000000 INFO @ Tue, 02 Aug 2022 16:22:34: 23000000 INFO @ Tue, 02 Aug 2022 16:22:40: 27000000 INFO @ Tue, 02 Aug 2022 16:22:41: 19000000 INFO @ Tue, 02 Aug 2022 16:22:42: 24000000 INFO @ Tue, 02 Aug 2022 16:22:49: 20000000 INFO @ Tue, 02 Aug 2022 16:22:49: 28000000 INFO @ Tue, 02 Aug 2022 16:22:49: 25000000 INFO @ Tue, 02 Aug 2022 16:22:57: 21000000 INFO @ Tue, 02 Aug 2022 16:22:57: 26000000 INFO @ Tue, 02 Aug 2022 16:22:59: 29000000 INFO @ Tue, 02 Aug 2022 16:23:05: 27000000 INFO @ Tue, 02 Aug 2022 16:23:05: 22000000 INFO @ Tue, 02 Aug 2022 16:23:07: 30000000 INFO @ Tue, 02 Aug 2022 16:23:12: 28000000 INFO @ Tue, 02 Aug 2022 16:23:13: 23000000 INFO @ Tue, 02 Aug 2022 16:23:16: 31000000 INFO @ Tue, 02 Aug 2022 16:23:20: 29000000 INFO @ Tue, 02 Aug 2022 16:23:20: 24000000 INFO @ Tue, 02 Aug 2022 16:23:24: 32000000 INFO @ Tue, 02 Aug 2022 16:23:27: 30000000 INFO @ Tue, 02 Aug 2022 16:23:28: 25000000 INFO @ Tue, 02 Aug 2022 16:23:33: 33000000 INFO @ Tue, 02 Aug 2022 16:23:34: 31000000 INFO @ Tue, 02 Aug 2022 16:23:35: 26000000 INFO @ Tue, 02 Aug 2022 16:23:41: 32000000 INFO @ Tue, 02 Aug 2022 16:23:42: 34000000 INFO @ Tue, 02 Aug 2022 16:23:44: 27000000 INFO @ Tue, 02 Aug 2022 16:23:48: 33000000 INFO @ Tue, 02 Aug 2022 16:23:50: 35000000 INFO @ Tue, 02 Aug 2022 16:23:52: 28000000 INFO @ Tue, 02 Aug 2022 16:23:55: 34000000 INFO @ Tue, 02 Aug 2022 16:23:58: 36000000 INFO @ Tue, 02 Aug 2022 16:24:01: 29000000 INFO @ Tue, 02 Aug 2022 16:24:02: 35000000 INFO @ Tue, 02 Aug 2022 16:24:07: 37000000 INFO @ Tue, 02 Aug 2022 16:24:09: 30000000 INFO @ Tue, 02 Aug 2022 16:24:09: 36000000 INFO @ Tue, 02 Aug 2022 16:24:15: 38000000 INFO @ Tue, 02 Aug 2022 16:24:16: 37000000 INFO @ Tue, 02 Aug 2022 16:24:17: 31000000 INFO @ Tue, 02 Aug 2022 16:24:23: 38000000 INFO @ Tue, 02 Aug 2022 16:24:24: 39000000 INFO @ Tue, 02 Aug 2022 16:24:24: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:24:31: 39000000 INFO @ Tue, 02 Aug 2022 16:24:33: 33000000 INFO @ Tue, 02 Aug 2022 16:24:33: 40000000 INFO @ Tue, 02 Aug 2022 16:24:39: 40000000 INFO @ Tue, 02 Aug 2022 16:24:40: 34000000 INFO @ Tue, 02 Aug 2022 16:24:41: 41000000 INFO @ Tue, 02 Aug 2022 16:24:46: 41000000 INFO @ Tue, 02 Aug 2022 16:24:48: 35000000 INFO @ Tue, 02 Aug 2022 16:24:50: 42000000 INFO @ Tue, 02 Aug 2022 16:24:54: 42000000 INFO @ Tue, 02 Aug 2022 16:24:57: 36000000 INFO @ Tue, 02 Aug 2022 16:24:58: 43000000 INFO @ Tue, 02 Aug 2022 16:25:01: 43000000 INFO @ Tue, 02 Aug 2022 16:25:06: 37000000 INFO @ Tue, 02 Aug 2022 16:25:07: 44000000 INFO @ Tue, 02 Aug 2022 16:25:08: 44000000 INFO @ Tue, 02 Aug 2022 16:25:16: 38000000 INFO @ Tue, 02 Aug 2022 16:25:16: 45000000 INFO @ Tue, 02 Aug 2022 16:25:17: 45000000 INFO @ Tue, 02 Aug 2022 16:25:23: 46000000 INFO @ Tue, 02 Aug 2022 16:25:24: 39000000 INFO @ Tue, 02 Aug 2022 16:25:26: 46000000 INFO @ Tue, 02 Aug 2022 16:25:31: 47000000 INFO @ Tue, 02 Aug 2022 16:25:32: 40000000 INFO @ Tue, 02 Aug 2022 16:25:34: 47000000 INFO @ Tue, 02 Aug 2022 16:25:37: 48000000 INFO @ Tue, 02 Aug 2022 16:25:41: 41000000 INFO @ Tue, 02 Aug 2022 16:25:42: 48000000 INFO @ Tue, 02 Aug 2022 16:25:45: 49000000 INFO @ Tue, 02 Aug 2022 16:25:50: 49000000 INFO @ Tue, 02 Aug 2022 16:25:51: 42000000 INFO @ Tue, 02 Aug 2022 16:25:52: 50000000 INFO @ Tue, 02 Aug 2022 16:25:54: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:25:54: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:25:54: #1 total tags in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:25:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:25:54: #1 tags after filtering in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:25:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:25:54: #1 finished! INFO @ Tue, 02 Aug 2022 16:25:54: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:25:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:25:57: 50000000 INFO @ Tue, 02 Aug 2022 16:25:58: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:25:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:25:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:25:59: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:25:59: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:25:59: #1 total tags in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:25:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:25:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:26:00: #1 tags after filtering in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:26:00: #1 finished! INFO @ Tue, 02 Aug 2022 16:26:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:26:01: 43000000 INFO @ Tue, 02 Aug 2022 16:26:03: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:26:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:26:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:26:09: 44000000 INFO @ Tue, 02 Aug 2022 16:26:18: 45000000 INFO @ Tue, 02 Aug 2022 16:26:25: 46000000 INFO @ Tue, 02 Aug 2022 16:26:31: 47000000 INFO @ Tue, 02 Aug 2022 16:26:38: 48000000 INFO @ Tue, 02 Aug 2022 16:26:45: 49000000 INFO @ Tue, 02 Aug 2022 16:26:52: 50000000 INFO @ Tue, 02 Aug 2022 16:26:54: #1 tag size is determined as 49 bps INFO @ Tue, 02 Aug 2022 16:26:54: #1 tag size = 49 INFO @ Tue, 02 Aug 2022 16:26:54: #1 total tags in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:26:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:26:54: #1 tags after filtering in treatment: 50207830 INFO @ Tue, 02 Aug 2022 16:26:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:26:54: #1 finished! INFO @ Tue, 02 Aug 2022 16:26:54: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:26:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:26:58: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:26:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:26:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13572053/SRX13572053.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling