Job ID = 1293883 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 127,252,800 reads read : 127,252,800 reads written : 127,252,800 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:25 127252800 reads; of these: 127252800 (100.00%) were unpaired; of these: 11621596 (9.13%) aligned 0 times 89995135 (70.72%) aligned exactly 1 time 25636069 (20.15%) aligned >1 times 90.87% overall alignment rate Time searching: 00:40:25 Overall time: 00:40:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 67052660 / 115631204 = 0.5799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:15:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:15:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:15:25: 1000000 INFO @ Mon, 03 Jun 2019 04:15:25: 1000000 INFO @ Mon, 03 Jun 2019 04:15:26: 1000000 INFO @ Mon, 03 Jun 2019 04:15:34: 2000000 INFO @ Mon, 03 Jun 2019 04:15:34: 2000000 INFO @ Mon, 03 Jun 2019 04:15:36: 2000000 INFO @ Mon, 03 Jun 2019 04:15:43: 3000000 INFO @ Mon, 03 Jun 2019 04:15:43: 3000000 INFO @ Mon, 03 Jun 2019 04:15:46: 3000000 INFO @ Mon, 03 Jun 2019 04:15:51: 4000000 INFO @ Mon, 03 Jun 2019 04:15:52: 4000000 INFO @ Mon, 03 Jun 2019 04:15:56: 4000000 INFO @ Mon, 03 Jun 2019 04:16:01: 5000000 INFO @ Mon, 03 Jun 2019 04:16:01: 5000000 INFO @ Mon, 03 Jun 2019 04:16:06: 5000000 INFO @ Mon, 03 Jun 2019 04:16:09: 6000000 INFO @ Mon, 03 Jun 2019 04:16:10: 6000000 INFO @ Mon, 03 Jun 2019 04:16:16: 6000000 INFO @ Mon, 03 Jun 2019 04:16:19: 7000000 INFO @ Mon, 03 Jun 2019 04:16:19: 7000000 INFO @ Mon, 03 Jun 2019 04:16:25: 7000000 INFO @ Mon, 03 Jun 2019 04:16:28: 8000000 INFO @ Mon, 03 Jun 2019 04:16:28: 8000000 INFO @ Mon, 03 Jun 2019 04:16:35: 8000000 INFO @ Mon, 03 Jun 2019 04:16:37: 9000000 INFO @ Mon, 03 Jun 2019 04:16:37: 9000000 INFO @ Mon, 03 Jun 2019 04:16:45: 9000000 INFO @ Mon, 03 Jun 2019 04:16:46: 10000000 INFO @ Mon, 03 Jun 2019 04:16:46: 10000000 INFO @ Mon, 03 Jun 2019 04:16:55: 11000000 INFO @ Mon, 03 Jun 2019 04:16:55: 11000000 INFO @ Mon, 03 Jun 2019 04:16:55: 10000000 INFO @ Mon, 03 Jun 2019 04:17:04: 12000000 INFO @ Mon, 03 Jun 2019 04:17:04: 12000000 INFO @ Mon, 03 Jun 2019 04:17:05: 11000000 INFO @ Mon, 03 Jun 2019 04:17:13: 13000000 INFO @ Mon, 03 Jun 2019 04:17:13: 13000000 INFO @ Mon, 03 Jun 2019 04:17:15: 12000000 INFO @ Mon, 03 Jun 2019 04:17:21: 14000000 INFO @ Mon, 03 Jun 2019 04:17:22: 14000000 INFO @ Mon, 03 Jun 2019 04:17:25: 13000000 INFO @ Mon, 03 Jun 2019 04:17:30: 15000000 INFO @ Mon, 03 Jun 2019 04:17:30: 15000000 INFO @ Mon, 03 Jun 2019 04:17:35: 14000000 INFO @ Mon, 03 Jun 2019 04:17:38: 16000000 INFO @ Mon, 03 Jun 2019 04:17:39: 16000000 INFO @ Mon, 03 Jun 2019 04:17:45: 15000000 INFO @ Mon, 03 Jun 2019 04:17:48: 17000000 INFO @ Mon, 03 Jun 2019 04:17:49: 17000000 INFO @ Mon, 03 Jun 2019 04:17:56: 16000000 INFO @ Mon, 03 Jun 2019 04:17:59: 18000000 INFO @ Mon, 03 Jun 2019 04:17:59: 18000000 INFO @ Mon, 03 Jun 2019 04:18:06: 17000000 INFO @ Mon, 03 Jun 2019 04:18:08: 19000000 INFO @ Mon, 03 Jun 2019 04:18:09: 19000000 INFO @ Mon, 03 Jun 2019 04:18:16: 18000000 INFO @ Mon, 03 Jun 2019 04:18:17: 20000000 INFO @ Mon, 03 Jun 2019 04:18:17: 20000000 INFO @ Mon, 03 Jun 2019 04:18:25: 21000000 INFO @ Mon, 03 Jun 2019 04:18:25: 19000000 INFO @ Mon, 03 Jun 2019 04:18:26: 21000000 INFO @ Mon, 03 Jun 2019 04:18:33: 22000000 INFO @ Mon, 03 Jun 2019 04:18:34: 22000000 INFO @ Mon, 03 Jun 2019 04:18:35: 20000000 INFO @ Mon, 03 Jun 2019 04:18:42: 23000000 INFO @ Mon, 03 Jun 2019 04:18:43: 23000000 INFO @ Mon, 03 Jun 2019 04:18:44: 21000000 INFO @ Mon, 03 Jun 2019 04:18:50: 24000000 INFO @ Mon, 03 Jun 2019 04:18:51: 24000000 INFO @ Mon, 03 Jun 2019 04:18:54: 22000000 INFO @ Mon, 03 Jun 2019 04:18:58: 25000000 INFO @ Mon, 03 Jun 2019 04:19:00: 25000000 INFO @ Mon, 03 Jun 2019 04:19:04: 23000000 INFO @ Mon, 03 Jun 2019 04:19:07: 26000000 INFO @ Mon, 03 Jun 2019 04:19:09: 26000000 INFO @ Mon, 03 Jun 2019 04:19:13: 24000000 INFO @ Mon, 03 Jun 2019 04:19:15: 27000000 INFO @ Mon, 03 Jun 2019 04:19:18: 27000000 INFO @ Mon, 03 Jun 2019 04:19:23: 25000000 INFO @ Mon, 03 Jun 2019 04:19:24: 28000000 INFO @ Mon, 03 Jun 2019 04:19:27: 28000000 INFO @ Mon, 03 Jun 2019 04:19:32: 29000000 INFO @ Mon, 03 Jun 2019 04:19:32: 26000000 INFO @ Mon, 03 Jun 2019 04:19:35: 29000000 INFO @ Mon, 03 Jun 2019 04:19:40: 30000000 INFO @ Mon, 03 Jun 2019 04:19:42: 27000000 INFO @ Mon, 03 Jun 2019 04:19:44: 30000000 INFO @ Mon, 03 Jun 2019 04:19:49: 31000000 INFO @ Mon, 03 Jun 2019 04:19:52: 28000000 INFO @ Mon, 03 Jun 2019 04:19:53: 31000000 INFO @ Mon, 03 Jun 2019 04:19:57: 32000000 INFO @ Mon, 03 Jun 2019 04:20:01: 29000000 INFO @ Mon, 03 Jun 2019 04:20:01: 32000000 INFO @ Mon, 03 Jun 2019 04:20:05: 33000000 INFO @ Mon, 03 Jun 2019 04:20:10: 33000000 INFO @ Mon, 03 Jun 2019 04:20:11: 30000000 INFO @ Mon, 03 Jun 2019 04:20:14: 34000000 INFO @ Mon, 03 Jun 2019 04:20:19: 34000000 INFO @ Mon, 03 Jun 2019 04:20:20: 31000000 INFO @ Mon, 03 Jun 2019 04:20:22: 35000000 INFO @ Mon, 03 Jun 2019 04:20:27: 35000000 INFO @ Mon, 03 Jun 2019 04:20:30: 32000000 INFO @ Mon, 03 Jun 2019 04:20:30: 36000000 INFO @ Mon, 03 Jun 2019 04:20:36: 36000000 INFO @ Mon, 03 Jun 2019 04:20:39: 37000000 INFO @ Mon, 03 Jun 2019 04:20:39: 33000000 INFO @ Mon, 03 Jun 2019 04:20:45: 37000000 INFO @ Mon, 03 Jun 2019 04:20:48: 38000000 INFO @ Mon, 03 Jun 2019 04:20:49: 34000000 INFO @ Mon, 03 Jun 2019 04:20:54: 38000000 INFO @ Mon, 03 Jun 2019 04:20:57: 39000000 INFO @ Mon, 03 Jun 2019 04:20:58: 35000000 INFO @ Mon, 03 Jun 2019 04:21:03: 39000000 INFO @ Mon, 03 Jun 2019 04:21:06: 40000000 INFO @ Mon, 03 Jun 2019 04:21:08: 36000000 INFO @ Mon, 03 Jun 2019 04:21:11: 40000000 INFO @ Mon, 03 Jun 2019 04:21:14: 41000000 INFO @ Mon, 03 Jun 2019 04:21:18: 37000000 INFO @ Mon, 03 Jun 2019 04:21:20: 41000000 INFO @ Mon, 03 Jun 2019 04:21:22: 42000000 INFO @ Mon, 03 Jun 2019 04:21:28: 38000000 INFO @ Mon, 03 Jun 2019 04:21:29: 42000000 INFO @ Mon, 03 Jun 2019 04:21:31: 43000000 INFO @ Mon, 03 Jun 2019 04:21:37: 39000000 INFO @ Mon, 03 Jun 2019 04:21:37: 43000000 INFO @ Mon, 03 Jun 2019 04:21:39: 44000000 INFO @ Mon, 03 Jun 2019 04:21:46: 44000000 INFO @ Mon, 03 Jun 2019 04:21:47: 40000000 INFO @ Mon, 03 Jun 2019 04:21:47: 45000000 INFO @ Mon, 03 Jun 2019 04:21:54: 45000000 INFO @ Mon, 03 Jun 2019 04:21:55: 46000000 INFO @ Mon, 03 Jun 2019 04:21:56: 41000000 INFO @ Mon, 03 Jun 2019 04:22:03: 46000000 INFO @ Mon, 03 Jun 2019 04:22:04: 47000000 INFO @ Mon, 03 Jun 2019 04:22:06: 42000000 INFO @ Mon, 03 Jun 2019 04:22:12: 47000000 INFO @ Mon, 03 Jun 2019 04:22:12: 48000000 INFO @ Mon, 03 Jun 2019 04:22:16: 43000000 INFO @ Mon, 03 Jun 2019 04:22:18: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:22:18: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:22:18: #1 total tags in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:22:18: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:22:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:22:19: #1 tags after filtering in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:22:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:22:19: #1 finished! INFO @ Mon, 03 Jun 2019 04:22:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:22:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:22:20: 48000000 INFO @ Mon, 03 Jun 2019 04:22:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:22:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:22:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:22:25: 44000000 INFO @ Mon, 03 Jun 2019 04:22:26: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:22:26: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:22:26: #1 total tags in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:22:26: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:22:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:22:27: #1 tags after filtering in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:22:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:22:27: #1 finished! INFO @ Mon, 03 Jun 2019 04:22:27: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:22:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:22:30: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:22:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:22:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:22:34: 45000000 INFO @ Mon, 03 Jun 2019 04:22:44: 46000000 INFO @ Mon, 03 Jun 2019 04:22:53: 47000000 INFO @ Mon, 03 Jun 2019 04:23:03: 48000000 INFO @ Mon, 03 Jun 2019 04:23:09: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 04:23:09: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 04:23:09: #1 total tags in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:23:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:23:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:23:10: #1 tags after filtering in treatment: 48578544 INFO @ Mon, 03 Jun 2019 04:23:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:23:10: #1 finished! INFO @ Mon, 03 Jun 2019 04:23:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:23:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:23:14: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 04:23:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 04:23:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135526/SRX135526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。