Job ID = 1293877 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 81,232,080 reads read : 81,232,080 reads written : 81,232,080 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:27 81232080 reads; of these: 81232080 (100.00%) were unpaired; of these: 5238016 (6.45%) aligned 0 times 69270277 (85.27%) aligned exactly 1 time 6723787 (8.28%) aligned >1 times 93.55% overall alignment rate Time searching: 00:20:27 Overall time: 00:20:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 37537780 / 75994064 = 0.4940 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 03:39:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:39:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:39:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:39:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:39:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:39:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:39:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:39:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:39:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:39:30: 1000000 INFO @ Mon, 03 Jun 2019 03:39:30: 1000000 INFO @ Mon, 03 Jun 2019 03:39:32: 1000000 INFO @ Mon, 03 Jun 2019 03:39:39: 2000000 INFO @ Mon, 03 Jun 2019 03:39:39: 2000000 INFO @ Mon, 03 Jun 2019 03:39:42: 2000000 INFO @ Mon, 03 Jun 2019 03:39:47: 3000000 INFO @ Mon, 03 Jun 2019 03:39:47: 3000000 INFO @ Mon, 03 Jun 2019 03:39:51: 3000000 INFO @ Mon, 03 Jun 2019 03:39:54: 4000000 INFO @ Mon, 03 Jun 2019 03:39:54: 4000000 INFO @ Mon, 03 Jun 2019 03:40:00: 4000000 INFO @ Mon, 03 Jun 2019 03:40:02: 5000000 INFO @ Mon, 03 Jun 2019 03:40:02: 5000000 INFO @ Mon, 03 Jun 2019 03:40:10: 5000000 INFO @ Mon, 03 Jun 2019 03:40:10: 6000000 INFO @ Mon, 03 Jun 2019 03:40:10: 6000000 INFO @ Mon, 03 Jun 2019 03:40:18: 7000000 INFO @ Mon, 03 Jun 2019 03:40:18: 7000000 INFO @ Mon, 03 Jun 2019 03:40:20: 6000000 INFO @ Mon, 03 Jun 2019 03:40:26: 8000000 INFO @ Mon, 03 Jun 2019 03:40:26: 8000000 INFO @ Mon, 03 Jun 2019 03:40:30: 7000000 INFO @ Mon, 03 Jun 2019 03:40:34: 9000000 INFO @ Mon, 03 Jun 2019 03:40:34: 9000000 INFO @ Mon, 03 Jun 2019 03:40:39: 8000000 INFO @ Mon, 03 Jun 2019 03:40:42: 10000000 INFO @ Mon, 03 Jun 2019 03:40:42: 10000000 INFO @ Mon, 03 Jun 2019 03:40:48: 9000000 INFO @ Mon, 03 Jun 2019 03:40:50: 11000000 INFO @ Mon, 03 Jun 2019 03:40:50: 11000000 INFO @ Mon, 03 Jun 2019 03:40:58: 12000000 INFO @ Mon, 03 Jun 2019 03:40:58: 12000000 INFO @ Mon, 03 Jun 2019 03:40:58: 10000000 INFO @ Mon, 03 Jun 2019 03:41:06: 13000000 INFO @ Mon, 03 Jun 2019 03:41:06: 13000000 INFO @ Mon, 03 Jun 2019 03:41:09: 11000000 INFO @ Mon, 03 Jun 2019 03:41:14: 14000000 INFO @ Mon, 03 Jun 2019 03:41:14: 14000000 INFO @ Mon, 03 Jun 2019 03:41:18: 12000000 INFO @ Mon, 03 Jun 2019 03:41:22: 15000000 INFO @ Mon, 03 Jun 2019 03:41:22: 15000000 INFO @ Mon, 03 Jun 2019 03:41:27: 13000000 INFO @ Mon, 03 Jun 2019 03:41:30: 16000000 INFO @ Mon, 03 Jun 2019 03:41:30: 16000000 INFO @ Mon, 03 Jun 2019 03:41:37: 14000000 INFO @ Mon, 03 Jun 2019 03:41:37: 17000000 INFO @ Mon, 03 Jun 2019 03:41:38: 17000000 INFO @ Mon, 03 Jun 2019 03:41:45: 18000000 INFO @ Mon, 03 Jun 2019 03:41:45: 18000000 INFO @ Mon, 03 Jun 2019 03:41:46: 15000000 INFO @ Mon, 03 Jun 2019 03:41:53: 19000000 INFO @ Mon, 03 Jun 2019 03:41:53: 19000000 INFO @ Mon, 03 Jun 2019 03:41:55: 16000000 INFO @ Mon, 03 Jun 2019 03:42:01: 20000000 INFO @ Mon, 03 Jun 2019 03:42:01: 20000000 INFO @ Mon, 03 Jun 2019 03:42:05: 17000000 INFO @ Mon, 03 Jun 2019 03:42:09: 21000000 INFO @ Mon, 03 Jun 2019 03:42:09: 21000000 INFO @ Mon, 03 Jun 2019 03:42:14: 18000000 INFO @ Mon, 03 Jun 2019 03:42:16: 22000000 INFO @ Mon, 03 Jun 2019 03:42:16: 22000000 INFO @ Mon, 03 Jun 2019 03:42:23: 19000000 INFO @ Mon, 03 Jun 2019 03:42:24: 23000000 INFO @ Mon, 03 Jun 2019 03:42:24: 23000000 INFO @ Mon, 03 Jun 2019 03:42:31: 24000000 INFO @ Mon, 03 Jun 2019 03:42:31: 24000000 INFO @ Mon, 03 Jun 2019 03:42:32: 20000000 INFO @ Mon, 03 Jun 2019 03:42:39: 25000000 INFO @ Mon, 03 Jun 2019 03:42:39: 25000000 INFO @ Mon, 03 Jun 2019 03:42:41: 21000000 INFO @ Mon, 03 Jun 2019 03:42:47: 26000000 INFO @ Mon, 03 Jun 2019 03:42:47: 26000000 INFO @ Mon, 03 Jun 2019 03:42:49: 22000000 INFO @ Mon, 03 Jun 2019 03:42:54: 27000000 INFO @ Mon, 03 Jun 2019 03:42:54: 27000000 INFO @ Mon, 03 Jun 2019 03:42:59: 23000000 INFO @ Mon, 03 Jun 2019 03:43:02: 28000000 INFO @ Mon, 03 Jun 2019 03:43:02: 28000000 INFO @ Mon, 03 Jun 2019 03:43:08: 24000000 INFO @ Mon, 03 Jun 2019 03:43:10: 29000000 INFO @ Mon, 03 Jun 2019 03:43:10: 29000000 INFO @ Mon, 03 Jun 2019 03:43:18: 30000000 INFO @ Mon, 03 Jun 2019 03:43:18: 30000000 INFO @ Mon, 03 Jun 2019 03:43:18: 25000000 INFO @ Mon, 03 Jun 2019 03:43:25: 31000000 INFO @ Mon, 03 Jun 2019 03:43:25: 31000000 INFO @ Mon, 03 Jun 2019 03:43:27: 26000000 INFO @ Mon, 03 Jun 2019 03:43:33: 32000000 INFO @ Mon, 03 Jun 2019 03:43:33: 32000000 INFO @ Mon, 03 Jun 2019 03:43:35: 27000000 INFO @ Mon, 03 Jun 2019 03:43:40: 33000000 INFO @ Mon, 03 Jun 2019 03:43:40: 33000000 INFO @ Mon, 03 Jun 2019 03:43:44: 28000000 INFO @ Mon, 03 Jun 2019 03:43:48: 34000000 INFO @ Mon, 03 Jun 2019 03:43:48: 34000000 INFO @ Mon, 03 Jun 2019 03:43:53: 29000000 INFO @ Mon, 03 Jun 2019 03:43:56: 35000000 INFO @ Mon, 03 Jun 2019 03:43:56: 35000000 INFO @ Mon, 03 Jun 2019 03:44:03: 30000000 INFO @ Mon, 03 Jun 2019 03:44:03: 36000000 INFO @ Mon, 03 Jun 2019 03:44:03: 36000000 INFO @ Mon, 03 Jun 2019 03:44:11: 37000000 INFO @ Mon, 03 Jun 2019 03:44:11: 37000000 INFO @ Mon, 03 Jun 2019 03:44:13: 31000000 INFO @ Mon, 03 Jun 2019 03:44:19: 38000000 INFO @ Mon, 03 Jun 2019 03:44:20: 38000000 INFO @ Mon, 03 Jun 2019 03:44:22: 32000000 INFO @ Mon, 03 Jun 2019 03:44:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:44:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:44:23: #1 total tags in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:44:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:44:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:44:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:44:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:44:23: #1 total tags in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:44:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:44:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:44:24: #1 tags after filtering in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:44:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:44:24: #1 finished! INFO @ Mon, 03 Jun 2019 03:44:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:44:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:44:24: #1 tags after filtering in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:44:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:44:24: #1 finished! INFO @ Mon, 03 Jun 2019 03:44:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:44:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:44:27: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 03:44:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:44:27: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 03:44:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:44:31: 33000000 INFO @ Mon, 03 Jun 2019 03:44:39: 34000000 INFO @ Mon, 03 Jun 2019 03:44:48: 35000000 INFO @ Mon, 03 Jun 2019 03:44:58: 36000000 INFO @ Mon, 03 Jun 2019 03:45:07: 37000000 INFO @ Mon, 03 Jun 2019 03:45:16: 38000000 INFO @ Mon, 03 Jun 2019 03:45:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:45:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:45:20: #1 total tags in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:45:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:45:21: #1 tags after filtering in treatment: 38456284 INFO @ Mon, 03 Jun 2019 03:45:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:45:21: #1 finished! INFO @ Mon, 03 Jun 2019 03:45:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:45:24: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 03:45:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:45:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX135520/SRX135520.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。