Job ID = 9158445 sra ファイルのダウンロード中... Completed: 8387709K bytes transferred in 72 seconds (946602K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 70788890 spots for /home/okishinya/chipatlas/results/dm3/SRX1342244/SRR2672979.sra Written 70788890 spots total Written 215346855 spots for /home/okishinya/chipatlas/results/dm3/SRX1342244/SRR2672980.sra Written 215346855 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:26:40 286135745 reads; of these: 286135745 (100.00%) were unpaired; of these: 57098273 (19.95%) aligned 0 times 180009151 (62.91%) aligned exactly 1 time 49028321 (17.13%) aligned >1 times 80.05% overall alignment rate Time searching: 01:26:40 Overall time: 01:26:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 96 files... [bam_rmdupse_core] 184270008 / 229037472 = 0.8045 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:10:24: # Command line: callpeak -t SRX1342244.bam -f BAM -g dm -n SRX1342244.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1342244.05 # format = BAM # ChIP-seq file = ['SRX1342244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:10:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:10:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:10:24: # Command line: callpeak -t SRX1342244.bam -f BAM -g dm -n SRX1342244.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1342244.20 # format = BAM # ChIP-seq file = ['SRX1342244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:10:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:10:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:10:24: # Command line: callpeak -t SRX1342244.bam -f BAM -g dm -n SRX1342244.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1342244.10 # format = BAM # ChIP-seq file = ['SRX1342244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:10:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:10:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:10:30: 1000000 INFO @ Tue, 27 Jun 2017 19:10:30: 1000000 INFO @ Tue, 27 Jun 2017 19:10:31: 1000000 INFO @ Tue, 27 Jun 2017 19:10:37: 2000000 INFO @ Tue, 27 Jun 2017 19:10:37: 2000000 INFO @ Tue, 27 Jun 2017 19:10:38: 2000000 INFO @ Tue, 27 Jun 2017 19:10:44: 3000000 INFO @ Tue, 27 Jun 2017 19:10:44: 3000000 INFO @ Tue, 27 Jun 2017 19:10:45: 3000000 INFO @ Tue, 27 Jun 2017 19:10:50: 4000000 INFO @ Tue, 27 Jun 2017 19:10:51: 4000000 INFO @ Tue, 27 Jun 2017 19:10:52: 4000000 INFO @ Tue, 27 Jun 2017 19:10:57: 5000000 INFO @ Tue, 27 Jun 2017 19:10:58: 5000000 INFO @ Tue, 27 Jun 2017 19:10:59: 5000000 INFO @ Tue, 27 Jun 2017 19:11:04: 6000000 INFO @ Tue, 27 Jun 2017 19:11:05: 6000000 INFO @ Tue, 27 Jun 2017 19:11:07: 6000000 INFO @ Tue, 27 Jun 2017 19:11:11: 7000000 INFO @ Tue, 27 Jun 2017 19:11:12: 7000000 INFO @ Tue, 27 Jun 2017 19:11:14: 7000000 INFO @ Tue, 27 Jun 2017 19:11:18: 8000000 INFO @ Tue, 27 Jun 2017 19:11:19: 8000000 INFO @ Tue, 27 Jun 2017 19:11:21: 8000000 INFO @ Tue, 27 Jun 2017 19:11:25: 9000000 INFO @ Tue, 27 Jun 2017 19:11:26: 9000000 INFO @ Tue, 27 Jun 2017 19:11:29: 9000000 INFO @ Tue, 27 Jun 2017 19:11:32: 10000000 INFO @ Tue, 27 Jun 2017 19:11:33: 10000000 INFO @ Tue, 27 Jun 2017 19:11:36: 10000000 INFO @ Tue, 27 Jun 2017 19:11:40: 11000000 INFO @ Tue, 27 Jun 2017 19:11:41: 11000000 INFO @ Tue, 27 Jun 2017 19:11:44: 11000000 INFO @ Tue, 27 Jun 2017 19:11:47: 12000000 INFO @ Tue, 27 Jun 2017 19:11:48: 12000000 INFO @ Tue, 27 Jun 2017 19:11:51: 12000000 INFO @ Tue, 27 Jun 2017 19:11:54: 13000000 INFO @ Tue, 27 Jun 2017 19:11:55: 13000000 INFO @ Tue, 27 Jun 2017 19:11:58: 13000000 INFO @ Tue, 27 Jun 2017 19:12:01: 14000000 INFO @ Tue, 27 Jun 2017 19:12:02: 14000000 INFO @ Tue, 27 Jun 2017 19:12:06: 14000000 INFO @ Tue, 27 Jun 2017 19:12:08: 15000000 INFO @ Tue, 27 Jun 2017 19:12:10: 15000000 INFO @ Tue, 27 Jun 2017 19:12:13: 15000000 INFO @ Tue, 27 Jun 2017 19:12:15: 16000000 INFO @ Tue, 27 Jun 2017 19:12:17: 16000000 INFO @ Tue, 27 Jun 2017 19:12:21: 16000000 INFO @ Tue, 27 Jun 2017 19:12:22: 17000000 INFO @ Tue, 27 Jun 2017 19:12:24: 17000000 INFO @ Tue, 27 Jun 2017 19:12:28: 17000000 INFO @ Tue, 27 Jun 2017 19:12:30: 18000000 INFO @ Tue, 27 Jun 2017 19:12:31: 18000000 INFO @ Tue, 27 Jun 2017 19:12:36: 18000000 INFO @ Tue, 27 Jun 2017 19:12:37: 19000000 INFO @ Tue, 27 Jun 2017 19:12:39: 19000000 INFO @ Tue, 27 Jun 2017 19:12:43: 19000000 INFO @ Tue, 27 Jun 2017 19:12:44: 20000000 INFO @ Tue, 27 Jun 2017 19:12:46: 20000000 INFO @ Tue, 27 Jun 2017 19:12:50: 20000000 INFO @ Tue, 27 Jun 2017 19:12:51: 21000000 INFO @ Tue, 27 Jun 2017 19:12:53: 21000000 INFO @ Tue, 27 Jun 2017 19:12:58: 21000000 INFO @ Tue, 27 Jun 2017 19:12:58: 22000000 INFO @ Tue, 27 Jun 2017 19:13:00: 22000000 INFO @ Tue, 27 Jun 2017 19:13:05: 23000000 INFO @ Tue, 27 Jun 2017 19:13:05: 22000000 INFO @ Tue, 27 Jun 2017 19:13:07: 23000000 INFO @ Tue, 27 Jun 2017 19:13:12: 24000000 INFO @ Tue, 27 Jun 2017 19:13:12: 23000000 INFO @ Tue, 27 Jun 2017 19:13:14: 24000000 INFO @ Tue, 27 Jun 2017 19:13:19: 25000000 INFO @ Tue, 27 Jun 2017 19:13:20: 24000000 INFO @ Tue, 27 Jun 2017 19:13:22: 25000000 INFO @ Tue, 27 Jun 2017 19:13:26: 26000000 INFO @ Tue, 27 Jun 2017 19:13:27: 25000000 INFO @ Tue, 27 Jun 2017 19:13:29: 26000000 INFO @ Tue, 27 Jun 2017 19:13:33: 27000000 INFO @ Tue, 27 Jun 2017 19:13:35: 26000000 INFO @ Tue, 27 Jun 2017 19:13:36: 27000000 INFO @ Tue, 27 Jun 2017 19:13:40: 28000000 INFO @ Tue, 27 Jun 2017 19:13:42: 27000000 INFO @ Tue, 27 Jun 2017 19:13:43: 28000000 INFO @ Tue, 27 Jun 2017 19:13:47: 29000000 INFO @ Tue, 27 Jun 2017 19:13:49: 28000000 INFO @ Tue, 27 Jun 2017 19:13:51: 29000000 INFO @ Tue, 27 Jun 2017 19:13:55: 30000000 INFO @ Tue, 27 Jun 2017 19:13:57: 29000000 INFO @ Tue, 27 Jun 2017 19:13:58: 30000000 INFO @ Tue, 27 Jun 2017 19:14:02: 31000000 INFO @ Tue, 27 Jun 2017 19:14:05: 30000000 INFO @ Tue, 27 Jun 2017 19:14:06: 31000000 INFO @ Tue, 27 Jun 2017 19:14:10: 32000000 INFO @ Tue, 27 Jun 2017 19:14:13: 31000000 INFO @ Tue, 27 Jun 2017 19:14:13: 32000000 INFO @ Tue, 27 Jun 2017 19:14:17: 33000000 INFO @ Tue, 27 Jun 2017 19:14:20: 32000000 INFO @ Tue, 27 Jun 2017 19:14:21: 33000000 INFO @ Tue, 27 Jun 2017 19:14:25: 34000000 INFO @ Tue, 27 Jun 2017 19:14:28: 33000000 INFO @ Tue, 27 Jun 2017 19:14:29: 34000000 INFO @ Tue, 27 Jun 2017 19:14:33: 35000000 INFO @ Tue, 27 Jun 2017 19:14:36: 34000000 INFO @ Tue, 27 Jun 2017 19:14:37: 35000000 INFO @ Tue, 27 Jun 2017 19:14:40: 36000000 INFO @ Tue, 27 Jun 2017 19:14:44: 35000000 INFO @ Tue, 27 Jun 2017 19:14:44: 36000000 INFO @ Tue, 27 Jun 2017 19:14:48: 37000000 INFO @ Tue, 27 Jun 2017 19:14:52: 36000000 INFO @ Tue, 27 Jun 2017 19:14:52: 37000000 INFO @ Tue, 27 Jun 2017 19:14:55: 38000000 INFO @ Tue, 27 Jun 2017 19:15:00: 37000000 INFO @ Tue, 27 Jun 2017 19:15:00: 38000000 INFO @ Tue, 27 Jun 2017 19:15:03: 39000000 INFO @ Tue, 27 Jun 2017 19:15:07: 39000000 INFO @ Tue, 27 Jun 2017 19:15:07: 38000000 INFO @ Tue, 27 Jun 2017 19:15:10: 40000000 INFO @ Tue, 27 Jun 2017 19:15:15: 40000000 INFO @ Tue, 27 Jun 2017 19:15:15: 39000000 INFO @ Tue, 27 Jun 2017 19:15:18: 41000000 INFO @ Tue, 27 Jun 2017 19:15:23: 41000000 INFO @ Tue, 27 Jun 2017 19:15:23: 40000000 INFO @ Tue, 27 Jun 2017 19:15:26: 42000000 INFO @ Tue, 27 Jun 2017 19:15:30: 42000000 INFO @ Tue, 27 Jun 2017 19:15:31: 41000000 INFO @ Tue, 27 Jun 2017 19:15:34: 43000000 INFO @ Tue, 27 Jun 2017 19:15:38: 43000000 INFO @ Tue, 27 Jun 2017 19:15:39: 42000000 INFO @ Tue, 27 Jun 2017 19:15:41: 44000000 INFO @ Tue, 27 Jun 2017 19:15:45: 44000000 INFO @ Tue, 27 Jun 2017 19:15:46: 43000000 INFO @ Tue, 27 Jun 2017 19:15:48: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:15:48: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:15:48: #1 total tags in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:15:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:15:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:15:49: #1 tags after filtering in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:15:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:15:49: #1 finished! INFO @ Tue, 27 Jun 2017 19:15:49: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:15:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:15:52: #2 number of paired peaks: 28 WARNING @ Tue, 27 Jun 2017 19:15:52: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:15:52: Process for pairing-model is terminated! cat: SRX1342244.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342244.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:15:52: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:15:52: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:15:52: #1 total tags in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:15:52: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:15:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:15:53: #1 tags after filtering in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:15:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:15:53: #1 finished! INFO @ Tue, 27 Jun 2017 19:15:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:15:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:15:53: 44000000 INFO @ Tue, 27 Jun 2017 19:15:55: #2 number of paired peaks: 28 WARNING @ Tue, 27 Jun 2017 19:15:55: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:15:55: Process for pairing-model is terminated! cat: SRX1342244.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342244.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:15:59: #1 tag size is determined as 61 bps INFO @ Tue, 27 Jun 2017 19:15:59: #1 tag size = 61 INFO @ Tue, 27 Jun 2017 19:15:59: #1 total tags in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:15:59: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:15:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:16:00: #1 tags after filtering in treatment: 44767464 INFO @ Tue, 27 Jun 2017 19:16:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:16:00: #1 finished! INFO @ Tue, 27 Jun 2017 19:16:00: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:16:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:16:03: #2 number of paired peaks: 28 WARNING @ Tue, 27 Jun 2017 19:16:03: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:16:03: Process for pairing-model is terminated! cat: SRX1342244.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342244.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342244.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。