Job ID = 9158443 sra ファイルのダウンロード中... Completed: 4995709K bytes transferred in 44 seconds (914613K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 65180940 spots for /home/okishinya/chipatlas/results/dm3/SRX1342243/SRR2672977.sra Written 65180940 spots total Written 88230860 spots for /home/okishinya/chipatlas/results/dm3/SRX1342243/SRR2672978.sra Written 88230860 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:52:19 153411800 reads; of these: 153411800 (100.00%) were unpaired; of these: 5998824 (3.91%) aligned 0 times 123150089 (80.27%) aligned exactly 1 time 24262887 (15.82%) aligned >1 times 96.09% overall alignment rate Time searching: 00:52:19 Overall time: 00:52:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 64 files... [bam_rmdupse_core] 80806749 / 147412976 = 0.5482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:17:17: # Command line: callpeak -t SRX1342243.bam -f BAM -g dm -n SRX1342243.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1342243.10 # format = BAM # ChIP-seq file = ['SRX1342243.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:17:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:17:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:17:17: # Command line: callpeak -t SRX1342243.bam -f BAM -g dm -n SRX1342243.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1342243.20 # format = BAM # ChIP-seq file = ['SRX1342243.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:17:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:17:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:17:17: # Command line: callpeak -t SRX1342243.bam -f BAM -g dm -n SRX1342243.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1342243.05 # format = BAM # ChIP-seq file = ['SRX1342243.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:17:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:17:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:17:26: 1000000 INFO @ Tue, 27 Jun 2017 18:17:26: 1000000 INFO @ Tue, 27 Jun 2017 18:17:26: 1000000 INFO @ Tue, 27 Jun 2017 18:17:35: 2000000 INFO @ Tue, 27 Jun 2017 18:17:35: 2000000 INFO @ Tue, 27 Jun 2017 18:17:35: 2000000 INFO @ Tue, 27 Jun 2017 18:17:44: 3000000 INFO @ Tue, 27 Jun 2017 18:17:44: 3000000 INFO @ Tue, 27 Jun 2017 18:17:45: 3000000 INFO @ Tue, 27 Jun 2017 18:17:53: 4000000 INFO @ Tue, 27 Jun 2017 18:17:53: 4000000 INFO @ Tue, 27 Jun 2017 18:17:53: 4000000 INFO @ Tue, 27 Jun 2017 18:18:02: 5000000 INFO @ Tue, 27 Jun 2017 18:18:03: 5000000 INFO @ Tue, 27 Jun 2017 18:18:03: 5000000 INFO @ Tue, 27 Jun 2017 18:18:11: 6000000 INFO @ Tue, 27 Jun 2017 18:18:12: 6000000 INFO @ Tue, 27 Jun 2017 18:18:12: 6000000 INFO @ Tue, 27 Jun 2017 18:18:20: 7000000 INFO @ Tue, 27 Jun 2017 18:18:20: 7000000 INFO @ Tue, 27 Jun 2017 18:18:21: 7000000 INFO @ Tue, 27 Jun 2017 18:18:28: 8000000 INFO @ Tue, 27 Jun 2017 18:18:29: 8000000 INFO @ Tue, 27 Jun 2017 18:18:29: 8000000 INFO @ Tue, 27 Jun 2017 18:18:37: 9000000 INFO @ Tue, 27 Jun 2017 18:18:38: 9000000 INFO @ Tue, 27 Jun 2017 18:18:38: 9000000 INFO @ Tue, 27 Jun 2017 18:18:46: 10000000 INFO @ Tue, 27 Jun 2017 18:18:47: 10000000 INFO @ Tue, 27 Jun 2017 18:18:47: 10000000 INFO @ Tue, 27 Jun 2017 18:18:54: 11000000 INFO @ Tue, 27 Jun 2017 18:18:56: 11000000 INFO @ Tue, 27 Jun 2017 18:18:56: 11000000 INFO @ Tue, 27 Jun 2017 18:19:03: 12000000 INFO @ Tue, 27 Jun 2017 18:19:05: 12000000 INFO @ Tue, 27 Jun 2017 18:19:05: 12000000 INFO @ Tue, 27 Jun 2017 18:19:11: 13000000 INFO @ Tue, 27 Jun 2017 18:19:14: 13000000 INFO @ Tue, 27 Jun 2017 18:19:14: 13000000 INFO @ Tue, 27 Jun 2017 18:19:20: 14000000 INFO @ Tue, 27 Jun 2017 18:19:22: 14000000 INFO @ Tue, 27 Jun 2017 18:19:23: 14000000 INFO @ Tue, 27 Jun 2017 18:19:29: 15000000 INFO @ Tue, 27 Jun 2017 18:19:31: 15000000 INFO @ Tue, 27 Jun 2017 18:19:32: 15000000 INFO @ Tue, 27 Jun 2017 18:19:37: 16000000 INFO @ Tue, 27 Jun 2017 18:19:40: 16000000 INFO @ Tue, 27 Jun 2017 18:19:40: 16000000 INFO @ Tue, 27 Jun 2017 18:19:46: 17000000 INFO @ Tue, 27 Jun 2017 18:19:49: 17000000 INFO @ Tue, 27 Jun 2017 18:19:49: 17000000 INFO @ Tue, 27 Jun 2017 18:19:55: 18000000 INFO @ Tue, 27 Jun 2017 18:19:58: 18000000 INFO @ Tue, 27 Jun 2017 18:19:58: 18000000 INFO @ Tue, 27 Jun 2017 18:20:03: 19000000 INFO @ Tue, 27 Jun 2017 18:20:08: 19000000 INFO @ Tue, 27 Jun 2017 18:20:08: 19000000 INFO @ Tue, 27 Jun 2017 18:20:12: 20000000 INFO @ Tue, 27 Jun 2017 18:20:17: 20000000 INFO @ Tue, 27 Jun 2017 18:20:17: 20000000 INFO @ Tue, 27 Jun 2017 18:20:20: 21000000 INFO @ Tue, 27 Jun 2017 18:20:26: 21000000 INFO @ Tue, 27 Jun 2017 18:20:26: 21000000 INFO @ Tue, 27 Jun 2017 18:20:28: 22000000 INFO @ Tue, 27 Jun 2017 18:20:34: 22000000 INFO @ Tue, 27 Jun 2017 18:20:34: 22000000 INFO @ Tue, 27 Jun 2017 18:20:37: 23000000 INFO @ Tue, 27 Jun 2017 18:20:43: 23000000 INFO @ Tue, 27 Jun 2017 18:20:43: 23000000 INFO @ Tue, 27 Jun 2017 18:20:45: 24000000 INFO @ Tue, 27 Jun 2017 18:20:52: 24000000 INFO @ Tue, 27 Jun 2017 18:20:52: 24000000 INFO @ Tue, 27 Jun 2017 18:20:54: 25000000 INFO @ Tue, 27 Jun 2017 18:21:01: 25000000 INFO @ Tue, 27 Jun 2017 18:21:01: 25000000 INFO @ Tue, 27 Jun 2017 18:21:02: 26000000 INFO @ Tue, 27 Jun 2017 18:21:10: 26000000 INFO @ Tue, 27 Jun 2017 18:21:10: 26000000 INFO @ Tue, 27 Jun 2017 18:21:11: 27000000 INFO @ Tue, 27 Jun 2017 18:21:18: 27000000 INFO @ Tue, 27 Jun 2017 18:21:19: 27000000 INFO @ Tue, 27 Jun 2017 18:21:19: 28000000 INFO @ Tue, 27 Jun 2017 18:21:27: 28000000 INFO @ Tue, 27 Jun 2017 18:21:27: 28000000 INFO @ Tue, 27 Jun 2017 18:21:28: 29000000 INFO @ Tue, 27 Jun 2017 18:21:36: 30000000 INFO @ Tue, 27 Jun 2017 18:21:36: 29000000 INFO @ Tue, 27 Jun 2017 18:21:36: 29000000 INFO @ Tue, 27 Jun 2017 18:21:44: 31000000 INFO @ Tue, 27 Jun 2017 18:21:45: 30000000 INFO @ Tue, 27 Jun 2017 18:21:45: 30000000 INFO @ Tue, 27 Jun 2017 18:21:53: 32000000 INFO @ Tue, 27 Jun 2017 18:21:54: 31000000 INFO @ Tue, 27 Jun 2017 18:21:54: 31000000 INFO @ Tue, 27 Jun 2017 18:22:01: 33000000 INFO @ Tue, 27 Jun 2017 18:22:02: 32000000 INFO @ Tue, 27 Jun 2017 18:22:02: 32000000 INFO @ Tue, 27 Jun 2017 18:22:09: 34000000 INFO @ Tue, 27 Jun 2017 18:22:11: 33000000 INFO @ Tue, 27 Jun 2017 18:22:11: 33000000 INFO @ Tue, 27 Jun 2017 18:22:18: 35000000 INFO @ Tue, 27 Jun 2017 18:22:20: 34000000 INFO @ Tue, 27 Jun 2017 18:22:20: 34000000 INFO @ Tue, 27 Jun 2017 18:22:26: 36000000 INFO @ Tue, 27 Jun 2017 18:22:28: 35000000 INFO @ Tue, 27 Jun 2017 18:22:28: 35000000 INFO @ Tue, 27 Jun 2017 18:22:34: 37000000 INFO @ Tue, 27 Jun 2017 18:22:37: 36000000 INFO @ Tue, 27 Jun 2017 18:22:37: 36000000 INFO @ Tue, 27 Jun 2017 18:22:43: 38000000 INFO @ Tue, 27 Jun 2017 18:22:46: 37000000 INFO @ Tue, 27 Jun 2017 18:22:46: 37000000 INFO @ Tue, 27 Jun 2017 18:22:51: 39000000 INFO @ Tue, 27 Jun 2017 18:22:55: 38000000 INFO @ Tue, 27 Jun 2017 18:22:55: 38000000 INFO @ Tue, 27 Jun 2017 18:23:00: 40000000 INFO @ Tue, 27 Jun 2017 18:23:03: 39000000 INFO @ Tue, 27 Jun 2017 18:23:03: 39000000 INFO @ Tue, 27 Jun 2017 18:23:08: 41000000 INFO @ Tue, 27 Jun 2017 18:23:12: 40000000 INFO @ Tue, 27 Jun 2017 18:23:12: 40000000 INFO @ Tue, 27 Jun 2017 18:23:17: 42000000 INFO @ Tue, 27 Jun 2017 18:23:21: 41000000 INFO @ Tue, 27 Jun 2017 18:23:21: 41000000 INFO @ Tue, 27 Jun 2017 18:23:25: 43000000 INFO @ Tue, 27 Jun 2017 18:23:30: 42000000 INFO @ Tue, 27 Jun 2017 18:23:30: 42000000 INFO @ Tue, 27 Jun 2017 18:23:33: 44000000 INFO @ Tue, 27 Jun 2017 18:23:39: 43000000 INFO @ Tue, 27 Jun 2017 18:23:39: 43000000 INFO @ Tue, 27 Jun 2017 18:23:42: 45000000 INFO @ Tue, 27 Jun 2017 18:23:47: 44000000 INFO @ Tue, 27 Jun 2017 18:23:47: 44000000 INFO @ Tue, 27 Jun 2017 18:23:50: 46000000 INFO @ Tue, 27 Jun 2017 18:23:56: 45000000 INFO @ Tue, 27 Jun 2017 18:23:56: 45000000 INFO @ Tue, 27 Jun 2017 18:23:59: 47000000 INFO @ Tue, 27 Jun 2017 18:24:05: 46000000 INFO @ Tue, 27 Jun 2017 18:24:05: 46000000 INFO @ Tue, 27 Jun 2017 18:24:07: 48000000 INFO @ Tue, 27 Jun 2017 18:24:14: 47000000 INFO @ Tue, 27 Jun 2017 18:24:14: 47000000 INFO @ Tue, 27 Jun 2017 18:24:15: 49000000 INFO @ Tue, 27 Jun 2017 18:24:22: 48000000 INFO @ Tue, 27 Jun 2017 18:24:22: 48000000 INFO @ Tue, 27 Jun 2017 18:24:23: 50000000 INFO @ Tue, 27 Jun 2017 18:24:31: 49000000 INFO @ Tue, 27 Jun 2017 18:24:31: 49000000 INFO @ Tue, 27 Jun 2017 18:24:32: 51000000 INFO @ Tue, 27 Jun 2017 18:24:39: 50000000 INFO @ Tue, 27 Jun 2017 18:24:39: 50000000 INFO @ Tue, 27 Jun 2017 18:24:40: 52000000 INFO @ Tue, 27 Jun 2017 18:24:48: 51000000 INFO @ Tue, 27 Jun 2017 18:24:48: 51000000 INFO @ Tue, 27 Jun 2017 18:24:48: 53000000 INFO @ Tue, 27 Jun 2017 18:24:57: 52000000 INFO @ Tue, 27 Jun 2017 18:24:57: 54000000 INFO @ Tue, 27 Jun 2017 18:24:58: 52000000 INFO @ Tue, 27 Jun 2017 18:25:06: 53000000 INFO @ Tue, 27 Jun 2017 18:25:07: 55000000 INFO @ Tue, 27 Jun 2017 18:25:10: 53000000 INFO @ Tue, 27 Jun 2017 18:25:16: 54000000 INFO @ Tue, 27 Jun 2017 18:25:17: 56000000 INFO @ Tue, 27 Jun 2017 18:25:22: 54000000 INFO @ Tue, 27 Jun 2017 18:25:26: 55000000 INFO @ Tue, 27 Jun 2017 18:25:27: 57000000 INFO @ Tue, 27 Jun 2017 18:25:34: 55000000 INFO @ Tue, 27 Jun 2017 18:25:36: 56000000 INFO @ Tue, 27 Jun 2017 18:25:37: 58000000 INFO @ Tue, 27 Jun 2017 18:25:45: 56000000 INFO @ Tue, 27 Jun 2017 18:25:46: 57000000 INFO @ Tue, 27 Jun 2017 18:25:47: 59000000 INFO @ Tue, 27 Jun 2017 18:25:55: 58000000 INFO @ Tue, 27 Jun 2017 18:25:57: 60000000 INFO @ Tue, 27 Jun 2017 18:25:57: 57000000 INFO @ Tue, 27 Jun 2017 18:26:05: 59000000 INFO @ Tue, 27 Jun 2017 18:26:07: 61000000 INFO @ Tue, 27 Jun 2017 18:26:09: 58000000 INFO @ Tue, 27 Jun 2017 18:26:15: 60000000 INFO @ Tue, 27 Jun 2017 18:26:17: 62000000 INFO @ Tue, 27 Jun 2017 18:26:21: 59000000 INFO @ Tue, 27 Jun 2017 18:26:25: 61000000 INFO @ Tue, 27 Jun 2017 18:26:28: 63000000 INFO @ Tue, 27 Jun 2017 18:26:33: 60000000 INFO @ Tue, 27 Jun 2017 18:26:35: 62000000 INFO @ Tue, 27 Jun 2017 18:26:38: 64000000 INFO @ Tue, 27 Jun 2017 18:26:45: 61000000 INFO @ Tue, 27 Jun 2017 18:26:46: 63000000 INFO @ Tue, 27 Jun 2017 18:26:49: 65000000 INFO @ Tue, 27 Jun 2017 18:26:56: 64000000 INFO @ Tue, 27 Jun 2017 18:26:56: 62000000 INFO @ Tue, 27 Jun 2017 18:27:01: 66000000 INFO @ Tue, 27 Jun 2017 18:27:06: 65000000 INFO @ Tue, 27 Jun 2017 18:27:07: 63000000 INFO @ Tue, 27 Jun 2017 18:27:09: #1 tag size is determined as 57 bps INFO @ Tue, 27 Jun 2017 18:27:09: #1 tag size = 57 INFO @ Tue, 27 Jun 2017 18:27:09: #1 total tags in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:09: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:27:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:27:10: #1 tags after filtering in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:27:10: #1 finished! INFO @ Tue, 27 Jun 2017 18:27:10: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:27:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:27:15: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:27:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:27:15: Process for pairing-model is terminated! cat: SRX1342243.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342243.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:27:16: 66000000 INFO @ Tue, 27 Jun 2017 18:27:19: 64000000 INFO @ Tue, 27 Jun 2017 18:27:23: #1 tag size is determined as 57 bps INFO @ Tue, 27 Jun 2017 18:27:23: #1 tag size = 57 INFO @ Tue, 27 Jun 2017 18:27:23: #1 total tags in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:23: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:27:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:27:24: #1 tags after filtering in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:27:24: #1 finished! INFO @ Tue, 27 Jun 2017 18:27:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:27:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:27:28: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:27:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:27:28: Process for pairing-model is terminated! cat: SRX1342243.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342243.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:27:29: 65000000 INFO @ Tue, 27 Jun 2017 18:27:40: 66000000 INFO @ Tue, 27 Jun 2017 18:27:47: #1 tag size is determined as 57 bps INFO @ Tue, 27 Jun 2017 18:27:47: #1 tag size = 57 INFO @ Tue, 27 Jun 2017 18:27:47: #1 total tags in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:27:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:27:49: #1 tags after filtering in treatment: 66606227 INFO @ Tue, 27 Jun 2017 18:27:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:27:49: #1 finished! INFO @ Tue, 27 Jun 2017 18:27:49: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:27:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:27:53: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:27:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:27:53: Process for pairing-model is terminated! cat: SRX1342243.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1342243.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1342243.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。