Job ID = 16440034 SRX = SRX13386240 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36265158 spots for SRR17206323/SRR17206323.sra Written 36265158 spots for SRR17206323/SRR17206323.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440748 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:58 36265158 reads; of these: 36265158 (100.00%) were paired; of these: 3162991 (8.72%) aligned concordantly 0 times 24885700 (68.62%) aligned concordantly exactly 1 time 8216467 (22.66%) aligned concordantly >1 times ---- 3162991 pairs aligned concordantly 0 times; of these: 34545 (1.09%) aligned discordantly 1 time ---- 3128446 pairs aligned 0 times concordantly or discordantly; of these: 6256892 mates make up the pairs; of these: 5392364 (86.18%) aligned 0 times 603762 (9.65%) aligned exactly 1 time 260766 (4.17%) aligned >1 times 92.57% overall alignment rate Time searching: 00:58:58 Overall time: 00:58:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8219496 / 33123095 = 0.2481 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:00:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:00:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:00:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:00:17: 1000000 INFO @ Tue, 02 Aug 2022 18:00:21: 2000000 INFO @ Tue, 02 Aug 2022 18:00:25: 3000000 INFO @ Tue, 02 Aug 2022 18:00:30: 4000000 INFO @ Tue, 02 Aug 2022 18:00:34: 5000000 INFO @ Tue, 02 Aug 2022 18:00:39: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:00:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:00:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:00:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:00:43: 7000000 INFO @ Tue, 02 Aug 2022 18:00:47: 1000000 INFO @ Tue, 02 Aug 2022 18:00:47: 8000000 INFO @ Tue, 02 Aug 2022 18:00:52: 9000000 INFO @ Tue, 02 Aug 2022 18:00:52: 2000000 INFO @ Tue, 02 Aug 2022 18:00:56: 10000000 INFO @ Tue, 02 Aug 2022 18:00:57: 3000000 INFO @ Tue, 02 Aug 2022 18:01:01: 11000000 INFO @ Tue, 02 Aug 2022 18:01:02: 4000000 INFO @ Tue, 02 Aug 2022 18:01:05: 12000000 INFO @ Tue, 02 Aug 2022 18:01:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:01:10: 13000000 INFO @ Tue, 02 Aug 2022 18:01:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:01:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:01:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:01:11: 6000000 INFO @ Tue, 02 Aug 2022 18:01:14: 14000000 INFO @ Tue, 02 Aug 2022 18:01:16: 1000000 INFO @ Tue, 02 Aug 2022 18:01:17: 7000000 INFO @ Tue, 02 Aug 2022 18:01:19: 15000000 INFO @ Tue, 02 Aug 2022 18:01:21: 2000000 INFO @ Tue, 02 Aug 2022 18:01:22: 8000000 INFO @ Tue, 02 Aug 2022 18:01:23: 16000000 INFO @ Tue, 02 Aug 2022 18:01:26: 3000000 INFO @ Tue, 02 Aug 2022 18:01:27: 9000000 INFO @ Tue, 02 Aug 2022 18:01:28: 17000000 INFO @ Tue, 02 Aug 2022 18:01:31: 4000000 INFO @ Tue, 02 Aug 2022 18:01:32: 10000000 INFO @ Tue, 02 Aug 2022 18:01:32: 18000000 INFO @ Tue, 02 Aug 2022 18:01:35: 5000000 INFO @ Tue, 02 Aug 2022 18:01:37: 19000000 INFO @ Tue, 02 Aug 2022 18:01:37: 11000000 INFO @ Tue, 02 Aug 2022 18:01:40: 6000000 INFO @ Tue, 02 Aug 2022 18:01:41: 20000000 INFO @ Tue, 02 Aug 2022 18:01:42: 12000000 INFO @ Tue, 02 Aug 2022 18:01:44: 7000000 INFO @ Tue, 02 Aug 2022 18:01:45: 21000000 INFO @ Tue, 02 Aug 2022 18:01:47: 13000000 INFO @ Tue, 02 Aug 2022 18:01:49: 8000000 INFO @ Tue, 02 Aug 2022 18:01:50: 22000000 INFO @ Tue, 02 Aug 2022 18:01:52: 14000000 INFO @ Tue, 02 Aug 2022 18:01:53: 9000000 INFO @ Tue, 02 Aug 2022 18:01:54: 23000000 INFO @ Tue, 02 Aug 2022 18:01:57: 15000000 INFO @ Tue, 02 Aug 2022 18:01:58: 10000000 INFO @ Tue, 02 Aug 2022 18:01:58: 24000000 INFO @ Tue, 02 Aug 2022 18:02:02: 16000000 INFO @ Tue, 02 Aug 2022 18:02:02: 11000000 INFO @ Tue, 02 Aug 2022 18:02:03: 25000000 INFO @ Tue, 02 Aug 2022 18:02:07: 12000000 INFO @ Tue, 02 Aug 2022 18:02:07: 17000000 INFO @ Tue, 02 Aug 2022 18:02:07: 26000000 INFO @ Tue, 02 Aug 2022 18:02:11: 27000000 INFO @ Tue, 02 Aug 2022 18:02:11: 13000000 INFO @ Tue, 02 Aug 2022 18:02:12: 18000000 INFO @ Tue, 02 Aug 2022 18:02:16: 28000000 INFO @ Tue, 02 Aug 2022 18:02:16: 14000000 INFO @ Tue, 02 Aug 2022 18:02:17: 19000000 INFO @ Tue, 02 Aug 2022 18:02:20: 29000000 INFO @ Tue, 02 Aug 2022 18:02:20: 15000000 INFO @ Tue, 02 Aug 2022 18:02:23: 20000000 INFO @ Tue, 02 Aug 2022 18:02:25: 30000000 INFO @ Tue, 02 Aug 2022 18:02:25: 16000000 INFO @ Tue, 02 Aug 2022 18:02:28: 21000000 INFO @ Tue, 02 Aug 2022 18:02:29: 31000000 INFO @ Tue, 02 Aug 2022 18:02:30: 17000000 INFO @ Tue, 02 Aug 2022 18:02:33: 22000000 INFO @ Tue, 02 Aug 2022 18:02:33: 32000000 INFO @ Tue, 02 Aug 2022 18:02:34: 18000000 INFO @ Tue, 02 Aug 2022 18:02:38: 33000000 INFO @ Tue, 02 Aug 2022 18:02:38: 23000000 INFO @ Tue, 02 Aug 2022 18:02:38: 19000000 INFO @ Tue, 02 Aug 2022 18:02:42: 34000000 INFO @ Tue, 02 Aug 2022 18:02:43: 20000000 INFO @ Tue, 02 Aug 2022 18:02:43: 24000000 INFO @ Tue, 02 Aug 2022 18:02:46: 35000000 INFO @ Tue, 02 Aug 2022 18:02:47: 21000000 INFO @ Tue, 02 Aug 2022 18:02:48: 25000000 INFO @ Tue, 02 Aug 2022 18:02:51: 36000000 INFO @ Tue, 02 Aug 2022 18:02:52: 22000000 INFO @ Tue, 02 Aug 2022 18:02:53: 26000000 INFO @ Tue, 02 Aug 2022 18:02:55: 37000000 INFO @ Tue, 02 Aug 2022 18:02:56: 23000000 INFO @ Tue, 02 Aug 2022 18:02:58: 27000000 INFO @ Tue, 02 Aug 2022 18:03:00: 38000000 INFO @ Tue, 02 Aug 2022 18:03:00: 24000000 INFO @ Tue, 02 Aug 2022 18:03:03: 28000000 INFO @ Tue, 02 Aug 2022 18:03:04: 39000000 INFO @ Tue, 02 Aug 2022 18:03:05: 25000000 INFO @ Tue, 02 Aug 2022 18:03:08: 29000000 INFO @ Tue, 02 Aug 2022 18:03:09: 40000000 INFO @ Tue, 02 Aug 2022 18:03:09: 26000000 INFO @ Tue, 02 Aug 2022 18:03:13: 30000000 INFO @ Tue, 02 Aug 2022 18:03:13: 41000000 INFO @ Tue, 02 Aug 2022 18:03:14: 27000000 INFO @ Tue, 02 Aug 2022 18:03:18: 42000000 INFO @ Tue, 02 Aug 2022 18:03:18: 31000000 INFO @ Tue, 02 Aug 2022 18:03:18: 28000000 INFO @ Tue, 02 Aug 2022 18:03:22: 43000000 INFO @ Tue, 02 Aug 2022 18:03:23: 29000000 INFO @ Tue, 02 Aug 2022 18:03:24: 32000000 INFO @ Tue, 02 Aug 2022 18:03:27: 44000000 INFO @ Tue, 02 Aug 2022 18:03:28: 30000000 INFO @ Tue, 02 Aug 2022 18:03:29: 33000000 INFO @ Tue, 02 Aug 2022 18:03:31: 45000000 INFO @ Tue, 02 Aug 2022 18:03:32: 31000000 INFO @ Tue, 02 Aug 2022 18:03:34: 34000000 INFO @ Tue, 02 Aug 2022 18:03:36: 46000000 INFO @ Tue, 02 Aug 2022 18:03:36: 32000000 INFO @ Tue, 02 Aug 2022 18:03:39: 35000000 INFO @ Tue, 02 Aug 2022 18:03:41: 47000000 INFO @ Tue, 02 Aug 2022 18:03:41: 33000000 INFO @ Tue, 02 Aug 2022 18:03:44: 36000000 INFO @ Tue, 02 Aug 2022 18:03:45: 48000000 INFO @ Tue, 02 Aug 2022 18:03:45: 34000000 INFO @ Tue, 02 Aug 2022 18:03:49: 37000000 INFO @ Tue, 02 Aug 2022 18:03:50: 49000000 INFO @ Tue, 02 Aug 2022 18:03:50: 35000000 INFO @ Tue, 02 Aug 2022 18:03:54: 36000000 INFO @ Tue, 02 Aug 2022 18:03:54: 50000000 INFO @ Tue, 02 Aug 2022 18:03:54: 38000000 INFO @ Tue, 02 Aug 2022 18:03:58: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:03:58: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:03:58: #1 total tags in treatment: 24886491 INFO @ Tue, 02 Aug 2022 18:03:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:03:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:03:58: #1 tags after filtering in treatment: 18744773 INFO @ Tue, 02 Aug 2022 18:03:58: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:03:58: #1 finished! INFO @ Tue, 02 Aug 2022 18:03:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:03:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:03:59: 37000000 INFO @ Tue, 02 Aug 2022 18:03:59: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:03:59: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:03:59: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 18:03:59: 39000000 cut: /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:04:03: 38000000 INFO @ Tue, 02 Aug 2022 18:04:05: 40000000 INFO @ Tue, 02 Aug 2022 18:04:08: 39000000 INFO @ Tue, 02 Aug 2022 18:04:10: 41000000 INFO @ Tue, 02 Aug 2022 18:04:13: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:04:15: 42000000 INFO @ Tue, 02 Aug 2022 18:04:17: 41000000 INFO @ Tue, 02 Aug 2022 18:04:20: 43000000 INFO @ Tue, 02 Aug 2022 18:04:22: 42000000 INFO @ Tue, 02 Aug 2022 18:04:25: 44000000 INFO @ Tue, 02 Aug 2022 18:04:26: 43000000 INFO @ Tue, 02 Aug 2022 18:04:30: 45000000 INFO @ Tue, 02 Aug 2022 18:04:31: 44000000 INFO @ Tue, 02 Aug 2022 18:04:35: 45000000 INFO @ Tue, 02 Aug 2022 18:04:35: 46000000 INFO @ Tue, 02 Aug 2022 18:04:40: 46000000 INFO @ Tue, 02 Aug 2022 18:04:40: 47000000 INFO @ Tue, 02 Aug 2022 18:04:44: 47000000 INFO @ Tue, 02 Aug 2022 18:04:45: 48000000 INFO @ Tue, 02 Aug 2022 18:04:49: 48000000 INFO @ Tue, 02 Aug 2022 18:04:50: 49000000 INFO @ Tue, 02 Aug 2022 18:04:53: 49000000 INFO @ Tue, 02 Aug 2022 18:04:55: 50000000 INFO @ Tue, 02 Aug 2022 18:04:58: 50000000 INFO @ Tue, 02 Aug 2022 18:04:59: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:04:59: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:04:59: #1 total tags in treatment: 24886491 INFO @ Tue, 02 Aug 2022 18:04:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:04:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:04:59: #1 tags after filtering in treatment: 18744773 INFO @ Tue, 02 Aug 2022 18:04:59: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:04:59: #1 finished! INFO @ Tue, 02 Aug 2022 18:04:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:04:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:05:01: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:05:01: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:05:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:05:01: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:05:01: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:05:01: #1 total tags in treatment: 24886491 INFO @ Tue, 02 Aug 2022 18:05:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:05:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:05:02: #1 tags after filtering in treatment: 18744773 INFO @ Tue, 02 Aug 2022 18:05:02: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:05:02: #1 finished! INFO @ Tue, 02 Aug 2022 18:05:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:05:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:05:03: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 18:05:03: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:05:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386240/SRX13386240.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。