Job ID = 16440014 SRX = SRX13386238 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 45365188 spots for SRR17206321/SRR17206321.sra Written 45365188 spots for SRR17206321/SRR17206321.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440999 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:39:38 45365188 reads; of these: 45365188 (100.00%) were paired; of these: 3762412 (8.29%) aligned concordantly 0 times 32488658 (71.62%) aligned concordantly exactly 1 time 9114118 (20.09%) aligned concordantly >1 times ---- 3762412 pairs aligned concordantly 0 times; of these: 38689 (1.03%) aligned discordantly 1 time ---- 3723723 pairs aligned 0 times concordantly or discordantly; of these: 7447446 mates make up the pairs; of these: 6463155 (86.78%) aligned 0 times 731830 (9.83%) aligned exactly 1 time 252461 (3.39%) aligned >1 times 92.88% overall alignment rate Time searching: 01:39:38 Overall time: 01:39:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 19909353 / 41622509 = 0.4783 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:42:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:42:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:42:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:42:44: 1000000 INFO @ Tue, 02 Aug 2022 18:42:51: 2000000 INFO @ Tue, 02 Aug 2022 18:42:58: 3000000 INFO @ Tue, 02 Aug 2022 18:43:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:43:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:43:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:43:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:43:12: 5000000 INFO @ Tue, 02 Aug 2022 18:43:16: 1000000 INFO @ Tue, 02 Aug 2022 18:43:18: 6000000 INFO @ Tue, 02 Aug 2022 18:43:25: 2000000 INFO @ Tue, 02 Aug 2022 18:43:25: 7000000 INFO @ Tue, 02 Aug 2022 18:43:32: 8000000 INFO @ Tue, 02 Aug 2022 18:43:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:43:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:43:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:43:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:43:39: 9000000 INFO @ Tue, 02 Aug 2022 18:43:42: 4000000 INFO @ Tue, 02 Aug 2022 18:43:46: 10000000 INFO @ Tue, 02 Aug 2022 18:43:48: 1000000 INFO @ Tue, 02 Aug 2022 18:43:51: 5000000 INFO @ Tue, 02 Aug 2022 18:43:53: 11000000 INFO @ Tue, 02 Aug 2022 18:43:58: 2000000 INFO @ Tue, 02 Aug 2022 18:43:59: 6000000 INFO @ Tue, 02 Aug 2022 18:44:00: 12000000 INFO @ Tue, 02 Aug 2022 18:44:07: 13000000 INFO @ Tue, 02 Aug 2022 18:44:09: 3000000 INFO @ Tue, 02 Aug 2022 18:44:09: 7000000 INFO @ Tue, 02 Aug 2022 18:44:15: 14000000 INFO @ Tue, 02 Aug 2022 18:44:18: 8000000 INFO @ Tue, 02 Aug 2022 18:44:19: 4000000 INFO @ Tue, 02 Aug 2022 18:44:22: 15000000 INFO @ Tue, 02 Aug 2022 18:44:27: 9000000 INFO @ Tue, 02 Aug 2022 18:44:29: 16000000 INFO @ Tue, 02 Aug 2022 18:44:30: 5000000 INFO @ Tue, 02 Aug 2022 18:44:36: 10000000 INFO @ Tue, 02 Aug 2022 18:44:38: 17000000 INFO @ Tue, 02 Aug 2022 18:44:40: 6000000 INFO @ Tue, 02 Aug 2022 18:44:44: 11000000 INFO @ Tue, 02 Aug 2022 18:44:45: 18000000 INFO @ Tue, 02 Aug 2022 18:44:51: 7000000 INFO @ Tue, 02 Aug 2022 18:44:52: 19000000 INFO @ Tue, 02 Aug 2022 18:44:53: 12000000 INFO @ Tue, 02 Aug 2022 18:44:59: 20000000 INFO @ Tue, 02 Aug 2022 18:45:01: 8000000 INFO @ Tue, 02 Aug 2022 18:45:02: 13000000 INFO @ Tue, 02 Aug 2022 18:45:07: 21000000 INFO @ Tue, 02 Aug 2022 18:45:11: 14000000 INFO @ Tue, 02 Aug 2022 18:45:12: 9000000 INFO @ Tue, 02 Aug 2022 18:45:14: 22000000 INFO @ Tue, 02 Aug 2022 18:45:20: 15000000 INFO @ Tue, 02 Aug 2022 18:45:21: 23000000 INFO @ Tue, 02 Aug 2022 18:45:21: 10000000 INFO @ Tue, 02 Aug 2022 18:45:28: 24000000 INFO @ Tue, 02 Aug 2022 18:45:29: 16000000 INFO @ Tue, 02 Aug 2022 18:45:30: 11000000 INFO @ Tue, 02 Aug 2022 18:45:35: 25000000 INFO @ Tue, 02 Aug 2022 18:45:38: 17000000 INFO @ Tue, 02 Aug 2022 18:45:39: 12000000 INFO @ Tue, 02 Aug 2022 18:45:43: 26000000 INFO @ Tue, 02 Aug 2022 18:45:47: 18000000 INFO @ Tue, 02 Aug 2022 18:45:48: 13000000 INFO @ Tue, 02 Aug 2022 18:45:50: 27000000 INFO @ Tue, 02 Aug 2022 18:45:56: 19000000 INFO @ Tue, 02 Aug 2022 18:45:58: 14000000 INFO @ Tue, 02 Aug 2022 18:46:00: 28000000 INFO @ Tue, 02 Aug 2022 18:46:05: 20000000 INFO @ Tue, 02 Aug 2022 18:46:09: 15000000 INFO @ Tue, 02 Aug 2022 18:46:11: 29000000 INFO @ Tue, 02 Aug 2022 18:46:15: 21000000 INFO @ Tue, 02 Aug 2022 18:46:21: 16000000 INFO @ Tue, 02 Aug 2022 18:46:21: 30000000 INFO @ Tue, 02 Aug 2022 18:46:26: 22000000 INFO @ Tue, 02 Aug 2022 18:46:29: 17000000 INFO @ Tue, 02 Aug 2022 18:46:32: 31000000 INFO @ Tue, 02 Aug 2022 18:46:36: 23000000 INFO @ Tue, 02 Aug 2022 18:46:37: 18000000 INFO @ Tue, 02 Aug 2022 18:46:43: 32000000 INFO @ Tue, 02 Aug 2022 18:46:44: 19000000 INFO @ Tue, 02 Aug 2022 18:46:50: 24000000 INFO @ Tue, 02 Aug 2022 18:46:52: 20000000 INFO @ Tue, 02 Aug 2022 18:46:53: 33000000 INFO @ Tue, 02 Aug 2022 18:47:00: 21000000 INFO @ Tue, 02 Aug 2022 18:47:03: 25000000 INFO @ Tue, 02 Aug 2022 18:47:04: 34000000 INFO @ Tue, 02 Aug 2022 18:47:08: 22000000 INFO @ Tue, 02 Aug 2022 18:47:15: 35000000 INFO @ Tue, 02 Aug 2022 18:47:16: 26000000 INFO @ Tue, 02 Aug 2022 18:47:16: 23000000 INFO @ Tue, 02 Aug 2022 18:47:24: 24000000 INFO @ Tue, 02 Aug 2022 18:47:26: 36000000 INFO @ Tue, 02 Aug 2022 18:47:27: 27000000 INFO @ Tue, 02 Aug 2022 18:47:32: 25000000 INFO @ Tue, 02 Aug 2022 18:47:38: 28000000 INFO @ Tue, 02 Aug 2022 18:47:38: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:47:40: 26000000 INFO @ Tue, 02 Aug 2022 18:47:48: 27000000 INFO @ Tue, 02 Aug 2022 18:47:49: 29000000 INFO @ Tue, 02 Aug 2022 18:47:49: 38000000 INFO @ Tue, 02 Aug 2022 18:47:56: 28000000 INFO @ Tue, 02 Aug 2022 18:48:00: 30000000 INFO @ Tue, 02 Aug 2022 18:48:01: 39000000 INFO @ Tue, 02 Aug 2022 18:48:04: 29000000 INFO @ Tue, 02 Aug 2022 18:48:11: 31000000 INFO @ Tue, 02 Aug 2022 18:48:12: 40000000 INFO @ Tue, 02 Aug 2022 18:48:12: 30000000 INFO @ Tue, 02 Aug 2022 18:48:20: 31000000 INFO @ Tue, 02 Aug 2022 18:48:22: 32000000 INFO @ Tue, 02 Aug 2022 18:48:23: 41000000 INFO @ Tue, 02 Aug 2022 18:48:28: 32000000 INFO @ Tue, 02 Aug 2022 18:48:34: 33000000 INFO @ Tue, 02 Aug 2022 18:48:34: 42000000 INFO @ Tue, 02 Aug 2022 18:48:36: 33000000 INFO @ Tue, 02 Aug 2022 18:48:44: 34000000 INFO @ Tue, 02 Aug 2022 18:48:45: 34000000 INFO @ Tue, 02 Aug 2022 18:48:45: 43000000 INFO @ Tue, 02 Aug 2022 18:48:52: 35000000 INFO @ Tue, 02 Aug 2022 18:48:56: 35000000 INFO @ Tue, 02 Aug 2022 18:48:56: 44000000 INFO @ Tue, 02 Aug 2022 18:49:00: 36000000 INFO @ Tue, 02 Aug 2022 18:49:01: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:49:01: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:49:01: #1 total tags in treatment: 21703951 INFO @ Tue, 02 Aug 2022 18:49:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:49:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:49:02: #1 tags after filtering in treatment: 16365303 INFO @ Tue, 02 Aug 2022 18:49:02: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:49:02: #1 finished! INFO @ Tue, 02 Aug 2022 18:49:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:49:03: #2 number of paired peaks: 75 WARNING @ Tue, 02 Aug 2022 18:49:03: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:49:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:49:07: 36000000 INFO @ Tue, 02 Aug 2022 18:49:08: 37000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:49:16: 38000000 INFO @ Tue, 02 Aug 2022 18:49:18: 37000000 INFO @ Tue, 02 Aug 2022 18:49:24: 39000000 INFO @ Tue, 02 Aug 2022 18:49:28: 38000000 INFO @ Tue, 02 Aug 2022 18:49:31: 40000000 INFO @ Tue, 02 Aug 2022 18:49:39: 41000000 INFO @ Tue, 02 Aug 2022 18:49:39: 39000000 INFO @ Tue, 02 Aug 2022 18:49:46: 42000000 INFO @ Tue, 02 Aug 2022 18:49:49: 40000000 INFO @ Tue, 02 Aug 2022 18:49:53: 43000000 INFO @ Tue, 02 Aug 2022 18:49:59: 41000000 INFO @ Tue, 02 Aug 2022 18:50:00: 44000000 INFO @ Tue, 02 Aug 2022 18:50:04: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:50:04: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:50:04: #1 total tags in treatment: 21703951 INFO @ Tue, 02 Aug 2022 18:50:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:50:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:50:04: #1 tags after filtering in treatment: 16365303 INFO @ Tue, 02 Aug 2022 18:50:04: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:50:04: #1 finished! INFO @ Tue, 02 Aug 2022 18:50:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:50:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:50:05: #2 number of paired peaks: 75 WARNING @ Tue, 02 Aug 2022 18:50:05: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:50:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:50:09: 42000000 INFO @ Tue, 02 Aug 2022 18:50:20: 43000000 INFO @ Tue, 02 Aug 2022 18:50:30: 44000000 INFO @ Tue, 02 Aug 2022 18:50:34: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 18:50:34: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 18:50:34: #1 total tags in treatment: 21703951 INFO @ Tue, 02 Aug 2022 18:50:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:50:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:50:35: #1 tags after filtering in treatment: 16365303 INFO @ Tue, 02 Aug 2022 18:50:35: #1 Redundant rate of treatment: 0.25 INFO @ Tue, 02 Aug 2022 18:50:35: #1 finished! INFO @ Tue, 02 Aug 2022 18:50:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:50:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:50:36: #2 number of paired peaks: 75 WARNING @ Tue, 02 Aug 2022 18:50:36: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:50:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13386238/SRX13386238.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling