Job ID = 16438941 SRX = SRX13227900 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 34635595 spots for SRR17038013/SRR17038013.sra Written 34635595 spots for SRR17038013/SRR17038013.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439161 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:23 34635595 reads; of these: 34635595 (100.00%) were unpaired; of these: 877256 (2.53%) aligned 0 times 24145561 (69.71%) aligned exactly 1 time 9612778 (27.75%) aligned >1 times 97.47% overall alignment rate Time searching: 00:20:24 Overall time: 00:20:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4332174 / 33758339 = 0.1283 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:47:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:47:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:47:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:48:08: 1000000 INFO @ Tue, 02 Aug 2022 14:48:18: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:48:28: 3000000 INFO @ Tue, 02 Aug 2022 14:48:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:48:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:48:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:48:38: 1000000 INFO @ Tue, 02 Aug 2022 14:48:38: 4000000 INFO @ Tue, 02 Aug 2022 14:48:47: 5000000 INFO @ Tue, 02 Aug 2022 14:48:47: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:48:57: 6000000 INFO @ Tue, 02 Aug 2022 14:48:57: 3000000 INFO @ Tue, 02 Aug 2022 14:48:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:48:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:48:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:49:07: 4000000 INFO @ Tue, 02 Aug 2022 14:49:07: 7000000 INFO @ Tue, 02 Aug 2022 14:49:08: 1000000 INFO @ Tue, 02 Aug 2022 14:49:18: 5000000 INFO @ Tue, 02 Aug 2022 14:49:18: 8000000 INFO @ Tue, 02 Aug 2022 14:49:18: 2000000 INFO @ Tue, 02 Aug 2022 14:49:28: 6000000 INFO @ Tue, 02 Aug 2022 14:49:28: 9000000 INFO @ Tue, 02 Aug 2022 14:49:29: 3000000 INFO @ Tue, 02 Aug 2022 14:49:38: 7000000 INFO @ Tue, 02 Aug 2022 14:49:39: 10000000 INFO @ Tue, 02 Aug 2022 14:49:40: 4000000 INFO @ Tue, 02 Aug 2022 14:49:48: 8000000 INFO @ Tue, 02 Aug 2022 14:49:50: 11000000 INFO @ Tue, 02 Aug 2022 14:49:51: 5000000 INFO @ Tue, 02 Aug 2022 14:49:58: 9000000 INFO @ Tue, 02 Aug 2022 14:50:01: 12000000 INFO @ Tue, 02 Aug 2022 14:50:02: 6000000 INFO @ Tue, 02 Aug 2022 14:50:09: 10000000 INFO @ Tue, 02 Aug 2022 14:50:12: 7000000 INFO @ Tue, 02 Aug 2022 14:50:13: 13000000 INFO @ Tue, 02 Aug 2022 14:50:20: 11000000 INFO @ Tue, 02 Aug 2022 14:50:23: 8000000 INFO @ Tue, 02 Aug 2022 14:50:23: 14000000 INFO @ Tue, 02 Aug 2022 14:50:31: 12000000 INFO @ Tue, 02 Aug 2022 14:50:33: 15000000 INFO @ Tue, 02 Aug 2022 14:50:34: 9000000 INFO @ Tue, 02 Aug 2022 14:50:42: 13000000 INFO @ Tue, 02 Aug 2022 14:50:43: 16000000 INFO @ Tue, 02 Aug 2022 14:50:45: 10000000 INFO @ Tue, 02 Aug 2022 14:50:53: 14000000 INFO @ Tue, 02 Aug 2022 14:50:53: 17000000 INFO @ Tue, 02 Aug 2022 14:50:56: 11000000 INFO @ Tue, 02 Aug 2022 14:51:03: 18000000 INFO @ Tue, 02 Aug 2022 14:51:03: 15000000 INFO @ Tue, 02 Aug 2022 14:51:07: 12000000 INFO @ Tue, 02 Aug 2022 14:51:12: 19000000 INFO @ Tue, 02 Aug 2022 14:51:13: 16000000 INFO @ Tue, 02 Aug 2022 14:51:18: 13000000 INFO @ Tue, 02 Aug 2022 14:51:22: 20000000 INFO @ Tue, 02 Aug 2022 14:51:24: 17000000 INFO @ Tue, 02 Aug 2022 14:51:28: 14000000 INFO @ Tue, 02 Aug 2022 14:51:32: 21000000 INFO @ Tue, 02 Aug 2022 14:51:34: 18000000 INFO @ Tue, 02 Aug 2022 14:51:38: 15000000 INFO @ Tue, 02 Aug 2022 14:51:42: 22000000 INFO @ Tue, 02 Aug 2022 14:51:44: 19000000 INFO @ Tue, 02 Aug 2022 14:51:48: 16000000 INFO @ Tue, 02 Aug 2022 14:51:52: 23000000 INFO @ Tue, 02 Aug 2022 14:51:54: 20000000 INFO @ Tue, 02 Aug 2022 14:51:58: 17000000 INFO @ Tue, 02 Aug 2022 14:52:02: 24000000 INFO @ Tue, 02 Aug 2022 14:52:03: 21000000 INFO @ Tue, 02 Aug 2022 14:52:09: 18000000 INFO @ Tue, 02 Aug 2022 14:52:13: 25000000 INFO @ Tue, 02 Aug 2022 14:52:13: 22000000 INFO @ Tue, 02 Aug 2022 14:52:19: 19000000 INFO @ Tue, 02 Aug 2022 14:52:22: 26000000 INFO @ Tue, 02 Aug 2022 14:52:23: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:52:28: 20000000 INFO @ Tue, 02 Aug 2022 14:52:32: 27000000 INFO @ Tue, 02 Aug 2022 14:52:33: 24000000 INFO @ Tue, 02 Aug 2022 14:52:38: 21000000 INFO @ Tue, 02 Aug 2022 14:52:42: 28000000 INFO @ Tue, 02 Aug 2022 14:52:42: 25000000 INFO @ Tue, 02 Aug 2022 14:52:47: 22000000 INFO @ Tue, 02 Aug 2022 14:52:51: 29000000 INFO @ Tue, 02 Aug 2022 14:52:52: 26000000 INFO @ Tue, 02 Aug 2022 14:52:55: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:52:55: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:52:55: #1 total tags in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:52:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:52:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:52:56: #1 tags after filtering in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:52:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:52:56: #1 finished! INFO @ Tue, 02 Aug 2022 14:52:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:52:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:52:57: 23000000 INFO @ Tue, 02 Aug 2022 14:52:58: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 14:52:58: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:52:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:53:02: 27000000 INFO @ Tue, 02 Aug 2022 14:53:07: 24000000 INFO @ Tue, 02 Aug 2022 14:53:12: 28000000 INFO @ Tue, 02 Aug 2022 14:53:16: 25000000 INFO @ Tue, 02 Aug 2022 14:53:21: 29000000 INFO @ Tue, 02 Aug 2022 14:53:25: 26000000 INFO @ Tue, 02 Aug 2022 14:53:26: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:53:26: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:53:26: #1 total tags in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:53:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:53:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:53:26: #1 tags after filtering in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:53:26: #1 finished! INFO @ Tue, 02 Aug 2022 14:53:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:53:29: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 14:53:29: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:53:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:53:35: 27000000 INFO @ Tue, 02 Aug 2022 14:53:45: 28000000 INFO @ Tue, 02 Aug 2022 14:53:54: 29000000 INFO @ Tue, 02 Aug 2022 14:53:59: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:53:59: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:53:59: #1 total tags in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:53:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:53:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:53:59: #1 tags after filtering in treatment: 29426165 INFO @ Tue, 02 Aug 2022 14:53:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:53:59: #1 finished! INFO @ Tue, 02 Aug 2022 14:53:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:53:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:54:02: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 14:54:02: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 14:54:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227900/SRX13227900.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。