Job ID = 16439338 SRX = SRX13227896 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36033252 spots for SRR17038009/SRR17038009.sra Written 36033252 spots for SRR17038009/SRR17038009.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439467 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:45 36033252 reads; of these: 36033252 (100.00%) were unpaired; of these: 924244 (2.56%) aligned 0 times 24948843 (69.24%) aligned exactly 1 time 10160165 (28.20%) aligned >1 times 97.44% overall alignment rate Time searching: 00:15:45 Overall time: 00:15:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4236939 / 35109008 = 0.1207 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:22:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:22:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:22:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:22:16: 1000000 INFO @ Tue, 02 Aug 2022 15:22:22: 2000000 INFO @ Tue, 02 Aug 2022 15:22:27: 3000000 INFO @ Tue, 02 Aug 2022 15:22:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:22:39: 5000000 INFO @ Tue, 02 Aug 2022 15:22:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:22:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:22:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:22:46: 6000000 INFO @ Tue, 02 Aug 2022 15:22:48: 1000000 INFO @ Tue, 02 Aug 2022 15:22:53: 7000000 INFO @ Tue, 02 Aug 2022 15:22:55: 2000000 INFO @ Tue, 02 Aug 2022 15:23:00: 8000000 INFO @ Tue, 02 Aug 2022 15:23:03: 3000000 INFO @ Tue, 02 Aug 2022 15:23:08: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:23:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:23:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:23:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:23:11: 4000000 INFO @ Tue, 02 Aug 2022 15:23:15: 10000000 INFO @ Tue, 02 Aug 2022 15:23:17: 1000000 INFO @ Tue, 02 Aug 2022 15:23:19: 5000000 INFO @ Tue, 02 Aug 2022 15:23:22: 11000000 INFO @ Tue, 02 Aug 2022 15:23:25: 2000000 INFO @ Tue, 02 Aug 2022 15:23:27: 6000000 INFO @ Tue, 02 Aug 2022 15:23:30: 12000000 INFO @ Tue, 02 Aug 2022 15:23:32: 3000000 INFO @ Tue, 02 Aug 2022 15:23:34: 7000000 INFO @ Tue, 02 Aug 2022 15:23:37: 13000000 INFO @ Tue, 02 Aug 2022 15:23:39: 4000000 INFO @ Tue, 02 Aug 2022 15:23:42: 8000000 INFO @ Tue, 02 Aug 2022 15:23:44: 14000000 INFO @ Tue, 02 Aug 2022 15:23:47: 5000000 INFO @ Tue, 02 Aug 2022 15:23:50: 9000000 INFO @ Tue, 02 Aug 2022 15:23:52: 15000000 INFO @ Tue, 02 Aug 2022 15:23:54: 6000000 INFO @ Tue, 02 Aug 2022 15:23:58: 10000000 INFO @ Tue, 02 Aug 2022 15:23:59: 16000000 INFO @ Tue, 02 Aug 2022 15:24:02: 7000000 INFO @ Tue, 02 Aug 2022 15:24:06: 11000000 INFO @ Tue, 02 Aug 2022 15:24:06: 17000000 INFO @ Tue, 02 Aug 2022 15:24:09: 8000000 INFO @ Tue, 02 Aug 2022 15:24:13: 18000000 INFO @ Tue, 02 Aug 2022 15:24:14: 12000000 INFO @ Tue, 02 Aug 2022 15:24:16: 9000000 INFO @ Tue, 02 Aug 2022 15:24:21: 19000000 INFO @ Tue, 02 Aug 2022 15:24:22: 13000000 INFO @ Tue, 02 Aug 2022 15:24:24: 10000000 INFO @ Tue, 02 Aug 2022 15:24:28: 20000000 INFO @ Tue, 02 Aug 2022 15:24:30: 14000000 INFO @ Tue, 02 Aug 2022 15:24:31: 11000000 INFO @ Tue, 02 Aug 2022 15:24:35: 21000000 INFO @ Tue, 02 Aug 2022 15:24:38: 15000000 INFO @ Tue, 02 Aug 2022 15:24:38: 12000000 INFO @ Tue, 02 Aug 2022 15:24:43: 22000000 INFO @ Tue, 02 Aug 2022 15:24:46: 16000000 INFO @ Tue, 02 Aug 2022 15:24:46: 13000000 INFO @ Tue, 02 Aug 2022 15:24:50: 23000000 INFO @ Tue, 02 Aug 2022 15:24:53: 14000000 INFO @ Tue, 02 Aug 2022 15:24:53: 17000000 INFO @ Tue, 02 Aug 2022 15:24:58: 24000000 INFO @ Tue, 02 Aug 2022 15:25:00: 15000000 INFO @ Tue, 02 Aug 2022 15:25:01: 18000000 INFO @ Tue, 02 Aug 2022 15:25:05: 25000000 INFO @ Tue, 02 Aug 2022 15:25:08: 16000000 INFO @ Tue, 02 Aug 2022 15:25:09: 19000000 INFO @ Tue, 02 Aug 2022 15:25:13: 26000000 INFO @ Tue, 02 Aug 2022 15:25:15: 17000000 INFO @ Tue, 02 Aug 2022 15:25:17: 20000000 INFO @ Tue, 02 Aug 2022 15:25:20: 27000000 INFO @ Tue, 02 Aug 2022 15:25:22: 18000000 INFO @ Tue, 02 Aug 2022 15:25:25: 21000000 INFO @ Tue, 02 Aug 2022 15:25:27: 28000000 INFO @ Tue, 02 Aug 2022 15:25:29: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:25:33: 22000000 INFO @ Tue, 02 Aug 2022 15:25:34: 29000000 INFO @ Tue, 02 Aug 2022 15:25:37: 20000000 INFO @ Tue, 02 Aug 2022 15:25:41: 23000000 INFO @ Tue, 02 Aug 2022 15:25:42: 30000000 INFO @ Tue, 02 Aug 2022 15:25:44: 21000000 INFO @ Tue, 02 Aug 2022 15:25:48: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:25:48: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:25:48: #1 total tags in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:25:48: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:25:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:25:49: #1 tags after filtering in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:25:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:25:49: #1 finished! INFO @ Tue, 02 Aug 2022 15:25:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:25:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:25:50: 24000000 INFO @ Tue, 02 Aug 2022 15:25:51: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 15:25:51: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:25:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:25:51: 22000000 INFO @ Tue, 02 Aug 2022 15:25:58: 25000000 INFO @ Tue, 02 Aug 2022 15:25:58: 23000000 INFO @ Tue, 02 Aug 2022 15:26:06: 24000000 INFO @ Tue, 02 Aug 2022 15:26:07: 26000000 INFO @ Tue, 02 Aug 2022 15:26:13: 25000000 INFO @ Tue, 02 Aug 2022 15:26:15: 27000000 INFO @ Tue, 02 Aug 2022 15:26:20: 26000000 INFO @ Tue, 02 Aug 2022 15:26:22: 28000000 INFO @ Tue, 02 Aug 2022 15:26:27: 27000000 INFO @ Tue, 02 Aug 2022 15:26:30: 29000000 INFO @ Tue, 02 Aug 2022 15:26:35: 28000000 INFO @ Tue, 02 Aug 2022 15:26:38: 30000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:26:42: 29000000 INFO @ Tue, 02 Aug 2022 15:26:45: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:26:45: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:26:45: #1 total tags in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:26:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:26:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:26:46: #1 tags after filtering in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:26:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:26:46: #1 finished! INFO @ Tue, 02 Aug 2022 15:26:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:26:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:26:47: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 15:26:47: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:26:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:26:49: 30000000 INFO @ Tue, 02 Aug 2022 15:26:54: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:26:54: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:26:54: #1 total tags in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:26:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:26:55: #1 tags after filtering in treatment: 30872069 INFO @ Tue, 02 Aug 2022 15:26:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:26:55: #1 finished! INFO @ Tue, 02 Aug 2022 15:26:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:26:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:26:57: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 15:26:57: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:26:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227896/SRX13227896.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling