Job ID = 16439335 SRX = SRX13227893 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33475241 spots for SRR17038006/SRR17038006.sra Written 33475241 spots for SRR17038006/SRR17038006.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439475 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:15 33475241 reads; of these: 33475241 (100.00%) were unpaired; of these: 1036078 (3.10%) aligned 0 times 23710225 (70.83%) aligned exactly 1 time 8728938 (26.08%) aligned >1 times 96.90% overall alignment rate Time searching: 00:17:15 Overall time: 00:17:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3925904 / 32439163 = 0.1210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:13: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:13: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:26:25: 1000000 INFO @ Tue, 02 Aug 2022 15:26:37: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:26:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:26:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:26:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:26:49: 3000000 INFO @ Tue, 02 Aug 2022 15:26:54: 1000000 INFO @ Tue, 02 Aug 2022 15:27:01: 4000000 INFO @ Tue, 02 Aug 2022 15:27:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:27:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:27:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:27:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:27:13: 5000000 INFO @ Tue, 02 Aug 2022 15:27:17: 3000000 INFO @ Tue, 02 Aug 2022 15:27:23: 1000000 INFO @ Tue, 02 Aug 2022 15:27:24: 6000000 INFO @ Tue, 02 Aug 2022 15:27:29: 4000000 INFO @ Tue, 02 Aug 2022 15:27:35: 7000000 INFO @ Tue, 02 Aug 2022 15:27:35: 2000000 INFO @ Tue, 02 Aug 2022 15:27:40: 5000000 INFO @ Tue, 02 Aug 2022 15:27:46: 8000000 INFO @ Tue, 02 Aug 2022 15:27:46: 3000000 INFO @ Tue, 02 Aug 2022 15:27:51: 6000000 INFO @ Tue, 02 Aug 2022 15:27:58: 9000000 INFO @ Tue, 02 Aug 2022 15:27:58: 4000000 INFO @ Tue, 02 Aug 2022 15:28:03: 7000000 INFO @ Tue, 02 Aug 2022 15:28:10: 10000000 INFO @ Tue, 02 Aug 2022 15:28:10: 5000000 INFO @ Tue, 02 Aug 2022 15:28:14: 8000000 INFO @ Tue, 02 Aug 2022 15:28:21: 11000000 INFO @ Tue, 02 Aug 2022 15:28:22: 6000000 INFO @ Tue, 02 Aug 2022 15:28:26: 9000000 INFO @ Tue, 02 Aug 2022 15:28:33: 12000000 INFO @ Tue, 02 Aug 2022 15:28:33: 7000000 INFO @ Tue, 02 Aug 2022 15:28:37: 10000000 INFO @ Tue, 02 Aug 2022 15:28:44: 13000000 INFO @ Tue, 02 Aug 2022 15:28:45: 8000000 INFO @ Tue, 02 Aug 2022 15:28:48: 11000000 INFO @ Tue, 02 Aug 2022 15:28:56: 14000000 INFO @ Tue, 02 Aug 2022 15:28:56: 9000000 INFO @ Tue, 02 Aug 2022 15:29:00: 12000000 INFO @ Tue, 02 Aug 2022 15:29:07: 15000000 INFO @ Tue, 02 Aug 2022 15:29:08: 10000000 INFO @ Tue, 02 Aug 2022 15:29:11: 13000000 INFO @ Tue, 02 Aug 2022 15:29:18: 16000000 INFO @ Tue, 02 Aug 2022 15:29:19: 11000000 INFO @ Tue, 02 Aug 2022 15:29:22: 14000000 INFO @ Tue, 02 Aug 2022 15:29:30: 17000000 INFO @ Tue, 02 Aug 2022 15:29:31: 12000000 INFO @ Tue, 02 Aug 2022 15:29:33: 15000000 INFO @ Tue, 02 Aug 2022 15:29:41: 18000000 INFO @ Tue, 02 Aug 2022 15:29:42: 13000000 INFO @ Tue, 02 Aug 2022 15:29:45: 16000000 INFO @ Tue, 02 Aug 2022 15:29:53: 19000000 INFO @ Tue, 02 Aug 2022 15:29:54: 14000000 INFO @ Tue, 02 Aug 2022 15:29:56: 17000000 INFO @ Tue, 02 Aug 2022 15:30:04: 20000000 INFO @ Tue, 02 Aug 2022 15:30:05: 15000000 INFO @ Tue, 02 Aug 2022 15:30:07: 18000000 INFO @ Tue, 02 Aug 2022 15:30:15: 21000000 INFO @ Tue, 02 Aug 2022 15:30:18: 19000000 INFO @ Tue, 02 Aug 2022 15:30:19: 16000000 INFO @ Tue, 02 Aug 2022 15:30:25: 22000000 INFO @ Tue, 02 Aug 2022 15:30:30: 20000000 INFO @ Tue, 02 Aug 2022 15:30:31: 17000000 INFO @ Tue, 02 Aug 2022 15:30:36: 23000000 INFO @ Tue, 02 Aug 2022 15:30:41: 21000000 INFO @ Tue, 02 Aug 2022 15:30:42: 18000000 INFO @ Tue, 02 Aug 2022 15:30:47: 24000000 INFO @ Tue, 02 Aug 2022 15:30:52: 22000000 INFO @ Tue, 02 Aug 2022 15:30:54: 19000000 INFO @ Tue, 02 Aug 2022 15:30:58: 25000000 INFO @ Tue, 02 Aug 2022 15:31:03: 23000000 INFO @ Tue, 02 Aug 2022 15:31:06: 20000000 INFO @ Tue, 02 Aug 2022 15:31:09: 26000000 INFO @ Tue, 02 Aug 2022 15:31:15: 24000000 INFO @ Tue, 02 Aug 2022 15:31:18: 21000000 INFO @ Tue, 02 Aug 2022 15:31:19: 27000000 INFO @ Tue, 02 Aug 2022 15:31:26: 25000000 INFO @ Tue, 02 Aug 2022 15:31:29: 22000000 INFO @ Tue, 02 Aug 2022 15:31:30: 28000000 INFO @ Tue, 02 Aug 2022 15:31:35: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:31:35: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:31:35: #1 total tags in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:31:35: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:31:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:31:36: #1 tags after filtering in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:31:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:31:36: #1 finished! INFO @ Tue, 02 Aug 2022 15:31:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:31:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:31:37: 26000000 INFO @ Tue, 02 Aug 2022 15:31:38: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:31:38: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:31:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:31:40: 23000000 INFO @ Tue, 02 Aug 2022 15:31:48: 27000000 INFO @ Tue, 02 Aug 2022 15:31:51: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:31:59: 28000000 INFO @ Tue, 02 Aug 2022 15:32:03: 25000000 INFO @ Tue, 02 Aug 2022 15:32:05: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:32:05: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:32:05: #1 total tags in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:32:05: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:32:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:32:06: #1 tags after filtering in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:32:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:32:06: #1 finished! INFO @ Tue, 02 Aug 2022 15:32:06: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:32:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:32:07: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:32:07: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:32:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:32:14: 26000000 INFO @ Tue, 02 Aug 2022 15:32:25: 27000000 INFO @ Tue, 02 Aug 2022 15:32:36: 28000000 INFO @ Tue, 02 Aug 2022 15:32:42: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:32:42: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:32:42: #1 total tags in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:32:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:32:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:32:42: #1 tags after filtering in treatment: 28513259 INFO @ Tue, 02 Aug 2022 15:32:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:32:42: #1 finished! INFO @ Tue, 02 Aug 2022 15:32:42: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:32:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:32:44: #2 number of paired peaks: 5 WARNING @ Tue, 02 Aug 2022 15:32:44: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:32:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227893/SRX13227893.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。