Job ID = 16439333 SRX = SRX13227891 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 38356879 spots for SRR17038004/SRR17038004.sra Written 38356879 spots for SRR17038004/SRR17038004.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439504 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:42 38356879 reads; of these: 38356879 (100.00%) were unpaired; of these: 1085406 (2.83%) aligned 0 times 27454125 (71.58%) aligned exactly 1 time 9817348 (25.59%) aligned >1 times 97.17% overall alignment rate Time searching: 00:21:42 Overall time: 00:21:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4603036 / 37271473 = 0.1235 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:34:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:34:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:34:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:34:38: 1000000 INFO @ Tue, 02 Aug 2022 15:34:47: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:34:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:34:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:34:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:34:57: 3000000 INFO @ Tue, 02 Aug 2022 15:35:06: 1000000 INFO @ Tue, 02 Aug 2022 15:35:07: 4000000 INFO @ Tue, 02 Aug 2022 15:35:16: 2000000 INFO @ Tue, 02 Aug 2022 15:35:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:35:25: 3000000 INFO @ Tue, 02 Aug 2022 15:35:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:35:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:35:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:35:26: 6000000 INFO @ Tue, 02 Aug 2022 15:35:35: 4000000 INFO @ Tue, 02 Aug 2022 15:35:36: 7000000 INFO @ Tue, 02 Aug 2022 15:35:37: 1000000 INFO @ Tue, 02 Aug 2022 15:35:44: 5000000 INFO @ Tue, 02 Aug 2022 15:35:46: 8000000 INFO @ Tue, 02 Aug 2022 15:35:48: 2000000 INFO @ Tue, 02 Aug 2022 15:35:54: 6000000 INFO @ Tue, 02 Aug 2022 15:35:55: 9000000 INFO @ Tue, 02 Aug 2022 15:35:59: 3000000 INFO @ Tue, 02 Aug 2022 15:36:03: 7000000 INFO @ Tue, 02 Aug 2022 15:36:04: 10000000 INFO @ Tue, 02 Aug 2022 15:36:11: 4000000 INFO @ Tue, 02 Aug 2022 15:36:12: 8000000 INFO @ Tue, 02 Aug 2022 15:36:14: 11000000 INFO @ Tue, 02 Aug 2022 15:36:21: 9000000 INFO @ Tue, 02 Aug 2022 15:36:21: 5000000 INFO @ Tue, 02 Aug 2022 15:36:23: 12000000 INFO @ Tue, 02 Aug 2022 15:36:30: 10000000 INFO @ Tue, 02 Aug 2022 15:36:32: 13000000 INFO @ Tue, 02 Aug 2022 15:36:33: 6000000 INFO @ Tue, 02 Aug 2022 15:36:39: 11000000 INFO @ Tue, 02 Aug 2022 15:36:41: 14000000 INFO @ Tue, 02 Aug 2022 15:36:44: 7000000 INFO @ Tue, 02 Aug 2022 15:36:49: 12000000 INFO @ Tue, 02 Aug 2022 15:36:49: 15000000 INFO @ Tue, 02 Aug 2022 15:36:55: 8000000 INFO @ Tue, 02 Aug 2022 15:36:58: 13000000 INFO @ Tue, 02 Aug 2022 15:36:58: 16000000 INFO @ Tue, 02 Aug 2022 15:37:06: 9000000 INFO @ Tue, 02 Aug 2022 15:37:06: 17000000 INFO @ Tue, 02 Aug 2022 15:37:07: 14000000 INFO @ Tue, 02 Aug 2022 15:37:15: 18000000 INFO @ Tue, 02 Aug 2022 15:37:16: 10000000 INFO @ Tue, 02 Aug 2022 15:37:17: 15000000 INFO @ Tue, 02 Aug 2022 15:37:23: 19000000 INFO @ Tue, 02 Aug 2022 15:37:26: 16000000 INFO @ Tue, 02 Aug 2022 15:37:26: 11000000 INFO @ Tue, 02 Aug 2022 15:37:32: 20000000 INFO @ Tue, 02 Aug 2022 15:37:35: 17000000 INFO @ Tue, 02 Aug 2022 15:37:37: 12000000 INFO @ Tue, 02 Aug 2022 15:37:41: 21000000 INFO @ Tue, 02 Aug 2022 15:37:44: 18000000 INFO @ Tue, 02 Aug 2022 15:37:47: 13000000 INFO @ Tue, 02 Aug 2022 15:37:49: 22000000 INFO @ Tue, 02 Aug 2022 15:37:53: 19000000 INFO @ Tue, 02 Aug 2022 15:37:57: 23000000 INFO @ Tue, 02 Aug 2022 15:37:58: 14000000 INFO @ Tue, 02 Aug 2022 15:38:01: 20000000 INFO @ Tue, 02 Aug 2022 15:38:05: 24000000 INFO @ Tue, 02 Aug 2022 15:38:08: 15000000 INFO @ Tue, 02 Aug 2022 15:38:10: 21000000 INFO @ Tue, 02 Aug 2022 15:38:13: 25000000 INFO @ Tue, 02 Aug 2022 15:38:18: 22000000 INFO @ Tue, 02 Aug 2022 15:38:19: 16000000 INFO @ Tue, 02 Aug 2022 15:38:22: 26000000 INFO @ Tue, 02 Aug 2022 15:38:27: 23000000 INFO @ Tue, 02 Aug 2022 15:38:29: 17000000 INFO @ Tue, 02 Aug 2022 15:38:31: 27000000 INFO @ Tue, 02 Aug 2022 15:38:35: 24000000 INFO @ Tue, 02 Aug 2022 15:38:39: 18000000 INFO @ Tue, 02 Aug 2022 15:38:39: 28000000 INFO @ Tue, 02 Aug 2022 15:38:43: 25000000 INFO @ Tue, 02 Aug 2022 15:38:47: 29000000 INFO @ Tue, 02 Aug 2022 15:38:48: 19000000 INFO @ Tue, 02 Aug 2022 15:38:51: 26000000 INFO @ Tue, 02 Aug 2022 15:38:56: 30000000 INFO @ Tue, 02 Aug 2022 15:38:59: 20000000 INFO @ Tue, 02 Aug 2022 15:39:00: 27000000 INFO @ Tue, 02 Aug 2022 15:39:04: 31000000 INFO @ Tue, 02 Aug 2022 15:39:08: 28000000 INFO @ Tue, 02 Aug 2022 15:39:09: 21000000 INFO @ Tue, 02 Aug 2022 15:39:11: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:39:16: 29000000 INFO @ Tue, 02 Aug 2022 15:39:17: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:39:17: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:39:17: #1 total tags in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:39:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:39:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:39:17: #1 tags after filtering in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:39:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:39:17: #1 finished! INFO @ Tue, 02 Aug 2022 15:39:17: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:39:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:39:20: 22000000 INFO @ Tue, 02 Aug 2022 15:39:20: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:39:20: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:39:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:39:24: 30000000 INFO @ Tue, 02 Aug 2022 15:39:29: 23000000 INFO @ Tue, 02 Aug 2022 15:39:32: 31000000 INFO @ Tue, 02 Aug 2022 15:39:39: 24000000 INFO @ Tue, 02 Aug 2022 15:39:39: 32000000 INFO @ Tue, 02 Aug 2022 15:39:45: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:39:45: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:39:45: #1 total tags in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:39:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:39:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:39:46: #1 tags after filtering in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:39:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:39:46: #1 finished! INFO @ Tue, 02 Aug 2022 15:39:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:39:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:39:48: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:39:48: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:39:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 15:39:48: 25000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:39:58: 26000000 INFO @ Tue, 02 Aug 2022 15:40:08: 27000000 INFO @ Tue, 02 Aug 2022 15:40:17: 28000000 INFO @ Tue, 02 Aug 2022 15:40:27: 29000000 INFO @ Tue, 02 Aug 2022 15:40:36: 30000000 INFO @ Tue, 02 Aug 2022 15:40:46: 31000000 INFO @ Tue, 02 Aug 2022 15:40:56: 32000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:41:03: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:41:03: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:41:03: #1 total tags in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:41:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:41:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:41:04: #1 tags after filtering in treatment: 32668437 INFO @ Tue, 02 Aug 2022 15:41:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:41:04: #1 finished! INFO @ Tue, 02 Aug 2022 15:41:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:41:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:41:06: #2 number of paired peaks: 3 WARNING @ Tue, 02 Aug 2022 15:41:06: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:41:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227891/SRX13227891.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling