Job ID = 16439324 SRX = SRX13227888 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 34081160 spots for SRR17038001/SRR17038001.sra Written 34081160 spots for SRR17038001/SRR17038001.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439499 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:56 34081160 reads; of these: 34081160 (100.00%) were unpaired; of these: 815954 (2.39%) aligned 0 times 23578627 (69.18%) aligned exactly 1 time 9686579 (28.42%) aligned >1 times 97.61% overall alignment rate Time searching: 00:21:56 Overall time: 00:21:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4076420 / 33265206 = 0.1225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:30:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:30:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:30:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:08: 1000000 INFO @ Tue, 02 Aug 2022 15:31:20: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:31:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:31:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:31:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:31:33: 3000000 INFO @ Tue, 02 Aug 2022 15:31:39: 1000000 INFO @ Tue, 02 Aug 2022 15:31:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:31:53: 2000000 INFO @ Tue, 02 Aug 2022 15:31:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:31:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:31:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:32:00: 5000000 INFO @ Tue, 02 Aug 2022 15:32:06: 3000000 INFO @ Tue, 02 Aug 2022 15:32:08: 1000000 INFO @ Tue, 02 Aug 2022 15:32:13: 6000000 INFO @ Tue, 02 Aug 2022 15:32:19: 4000000 INFO @ Tue, 02 Aug 2022 15:32:21: 2000000 INFO @ Tue, 02 Aug 2022 15:32:27: 7000000 INFO @ Tue, 02 Aug 2022 15:32:33: 5000000 INFO @ Tue, 02 Aug 2022 15:32:34: 3000000 INFO @ Tue, 02 Aug 2022 15:32:40: 8000000 INFO @ Tue, 02 Aug 2022 15:32:46: 6000000 INFO @ Tue, 02 Aug 2022 15:32:47: 4000000 INFO @ Tue, 02 Aug 2022 15:32:53: 9000000 INFO @ Tue, 02 Aug 2022 15:32:59: 7000000 INFO @ Tue, 02 Aug 2022 15:32:59: 5000000 INFO @ Tue, 02 Aug 2022 15:33:07: 10000000 INFO @ Tue, 02 Aug 2022 15:33:12: 6000000 INFO @ Tue, 02 Aug 2022 15:33:13: 8000000 INFO @ Tue, 02 Aug 2022 15:33:20: 11000000 INFO @ Tue, 02 Aug 2022 15:33:23: 7000000 INFO @ Tue, 02 Aug 2022 15:33:27: 9000000 INFO @ Tue, 02 Aug 2022 15:33:34: 12000000 INFO @ Tue, 02 Aug 2022 15:33:35: 8000000 INFO @ Tue, 02 Aug 2022 15:33:40: 10000000 INFO @ Tue, 02 Aug 2022 15:33:47: 13000000 INFO @ Tue, 02 Aug 2022 15:33:47: 9000000 INFO @ Tue, 02 Aug 2022 15:33:54: 11000000 INFO @ Tue, 02 Aug 2022 15:34:00: 10000000 INFO @ Tue, 02 Aug 2022 15:34:01: 14000000 INFO @ Tue, 02 Aug 2022 15:34:07: 12000000 INFO @ Tue, 02 Aug 2022 15:34:12: 11000000 INFO @ Tue, 02 Aug 2022 15:34:13: 15000000 INFO @ Tue, 02 Aug 2022 15:34:21: 13000000 INFO @ Tue, 02 Aug 2022 15:34:24: 12000000 INFO @ Tue, 02 Aug 2022 15:34:26: 16000000 INFO @ Tue, 02 Aug 2022 15:34:33: 14000000 INFO @ Tue, 02 Aug 2022 15:34:36: 13000000 INFO @ Tue, 02 Aug 2022 15:34:40: 17000000 INFO @ Tue, 02 Aug 2022 15:34:46: 15000000 INFO @ Tue, 02 Aug 2022 15:34:49: 14000000 INFO @ Tue, 02 Aug 2022 15:34:52: 18000000 INFO @ Tue, 02 Aug 2022 15:34:59: 16000000 INFO @ Tue, 02 Aug 2022 15:35:01: 15000000 INFO @ Tue, 02 Aug 2022 15:35:05: 19000000 INFO @ Tue, 02 Aug 2022 15:35:11: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:35:15: 16000000 INFO @ Tue, 02 Aug 2022 15:35:18: 20000000 INFO @ Tue, 02 Aug 2022 15:35:24: 18000000 INFO @ Tue, 02 Aug 2022 15:35:27: 17000000 INFO @ Tue, 02 Aug 2022 15:35:31: 21000000 INFO @ Tue, 02 Aug 2022 15:35:37: 19000000 INFO @ Tue, 02 Aug 2022 15:35:40: 18000000 INFO @ Tue, 02 Aug 2022 15:35:45: 22000000 INFO @ Tue, 02 Aug 2022 15:35:51: 20000000 INFO @ Tue, 02 Aug 2022 15:35:52: 19000000 INFO @ Tue, 02 Aug 2022 15:36:00: 23000000 INFO @ Tue, 02 Aug 2022 15:36:05: 20000000 INFO @ Tue, 02 Aug 2022 15:36:06: 21000000 INFO @ Tue, 02 Aug 2022 15:36:13: 24000000 INFO @ Tue, 02 Aug 2022 15:36:19: 22000000 INFO @ Tue, 02 Aug 2022 15:36:19: 21000000 INFO @ Tue, 02 Aug 2022 15:36:27: 25000000 INFO @ Tue, 02 Aug 2022 15:36:31: 23000000 INFO @ Tue, 02 Aug 2022 15:36:33: 22000000 INFO @ Tue, 02 Aug 2022 15:36:41: 26000000 INFO @ Tue, 02 Aug 2022 15:36:43: 24000000 INFO @ Tue, 02 Aug 2022 15:36:47: 23000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:36:56: 25000000 INFO @ Tue, 02 Aug 2022 15:36:56: 27000000 INFO @ Tue, 02 Aug 2022 15:37:00: 24000000 INFO @ Tue, 02 Aug 2022 15:37:09: 26000000 INFO @ Tue, 02 Aug 2022 15:37:09: 28000000 INFO @ Tue, 02 Aug 2022 15:37:13: 25000000 INFO @ Tue, 02 Aug 2022 15:37:23: 29000000 INFO @ Tue, 02 Aug 2022 15:37:23: 27000000 INFO @ Tue, 02 Aug 2022 15:37:25: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:37:25: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:37:25: #1 total tags in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:37:25: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:37:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:37:26: #1 tags after filtering in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:37:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:37:26: #1 finished! INFO @ Tue, 02 Aug 2022 15:37:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:37:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:37:26: 26000000 INFO @ Tue, 02 Aug 2022 15:37:28: #2 number of paired peaks: 54 WARNING @ Tue, 02 Aug 2022 15:37:28: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:37:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:37:36: 28000000 INFO @ Tue, 02 Aug 2022 15:37:39: 27000000 INFO @ Tue, 02 Aug 2022 15:37:47: 29000000 INFO @ Tue, 02 Aug 2022 15:37:49: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:37:49: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:37:49: #1 total tags in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:37:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:37:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:37:50: #1 tags after filtering in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:37:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:37:50: #1 finished! INFO @ Tue, 02 Aug 2022 15:37:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:37:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:37:52: #2 number of paired peaks: 54 WARNING @ Tue, 02 Aug 2022 15:37:52: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:37:53: 28000000 INFO @ Tue, 02 Aug 2022 15:38:05: 29000000 INFO @ Tue, 02 Aug 2022 15:38:08: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:38:08: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:38:08: #1 total tags in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:38:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:38:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:38:08: #1 tags after filtering in treatment: 29188786 INFO @ Tue, 02 Aug 2022 15:38:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:38:08: #1 finished! INFO @ Tue, 02 Aug 2022 15:38:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:38:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:38:11: #2 number of paired peaks: 54 WARNING @ Tue, 02 Aug 2022 15:38:11: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:38:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227888/SRX13227888.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling