Job ID = 16439323 SRX = SRX13227887 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36517190 spots for SRR17038000/SRR17038000.sra Written 36517190 spots for SRR17038000/SRR17038000.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439456 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:55 36517190 reads; of these: 36517190 (100.00%) were unpaired; of these: 1570213 (4.30%) aligned 0 times 25827820 (70.73%) aligned exactly 1 time 9119157 (24.97%) aligned >1 times 95.70% overall alignment rate Time searching: 00:15:55 Overall time: 00:15:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4051093 / 34946977 = 0.1159 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:20:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:20:13: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:20:13: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:20:20: 1000000 INFO @ Tue, 02 Aug 2022 15:20:28: 2000000 INFO @ Tue, 02 Aug 2022 15:20:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:20:41: 4000000 INFO @ Tue, 02 Aug 2022 15:20:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:20:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:20:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:20:50: 5000000 INFO @ Tue, 02 Aug 2022 15:20:51: 1000000 INFO @ Tue, 02 Aug 2022 15:20:58: 6000000 INFO @ Tue, 02 Aug 2022 15:21:00: 2000000 INFO @ Tue, 02 Aug 2022 15:21:06: 7000000 INFO @ Tue, 02 Aug 2022 15:21:08: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:21:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:21:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:21:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:21:14: 8000000 INFO @ Tue, 02 Aug 2022 15:21:17: 4000000 INFO @ Tue, 02 Aug 2022 15:21:22: 1000000 INFO @ Tue, 02 Aug 2022 15:21:23: 9000000 INFO @ Tue, 02 Aug 2022 15:21:25: 5000000 INFO @ Tue, 02 Aug 2022 15:21:31: 2000000 INFO @ Tue, 02 Aug 2022 15:21:32: 10000000 INFO @ Tue, 02 Aug 2022 15:21:34: 6000000 INFO @ Tue, 02 Aug 2022 15:21:40: 11000000 INFO @ Tue, 02 Aug 2022 15:21:41: 3000000 INFO @ Tue, 02 Aug 2022 15:21:43: 7000000 INFO @ Tue, 02 Aug 2022 15:21:49: 12000000 INFO @ Tue, 02 Aug 2022 15:21:51: 4000000 INFO @ Tue, 02 Aug 2022 15:21:51: 8000000 INFO @ Tue, 02 Aug 2022 15:21:57: 13000000 INFO @ Tue, 02 Aug 2022 15:22:00: 9000000 INFO @ Tue, 02 Aug 2022 15:22:00: 5000000 INFO @ Tue, 02 Aug 2022 15:22:05: 14000000 INFO @ Tue, 02 Aug 2022 15:22:08: 10000000 INFO @ Tue, 02 Aug 2022 15:22:10: 6000000 INFO @ Tue, 02 Aug 2022 15:22:14: 15000000 INFO @ Tue, 02 Aug 2022 15:22:17: 11000000 INFO @ Tue, 02 Aug 2022 15:22:19: 7000000 INFO @ Tue, 02 Aug 2022 15:22:22: 16000000 INFO @ Tue, 02 Aug 2022 15:22:26: 12000000 INFO @ Tue, 02 Aug 2022 15:22:29: 8000000 INFO @ Tue, 02 Aug 2022 15:22:31: 17000000 INFO @ Tue, 02 Aug 2022 15:22:34: 13000000 INFO @ Tue, 02 Aug 2022 15:22:38: 9000000 INFO @ Tue, 02 Aug 2022 15:22:40: 18000000 INFO @ Tue, 02 Aug 2022 15:22:43: 14000000 INFO @ Tue, 02 Aug 2022 15:22:48: 10000000 INFO @ Tue, 02 Aug 2022 15:22:48: 19000000 INFO @ Tue, 02 Aug 2022 15:22:52: 15000000 INFO @ Tue, 02 Aug 2022 15:22:57: 20000000 INFO @ Tue, 02 Aug 2022 15:22:57: 11000000 INFO @ Tue, 02 Aug 2022 15:23:01: 16000000 INFO @ Tue, 02 Aug 2022 15:23:05: 21000000 INFO @ Tue, 02 Aug 2022 15:23:07: 12000000 INFO @ Tue, 02 Aug 2022 15:23:10: 17000000 INFO @ Tue, 02 Aug 2022 15:23:14: 22000000 INFO @ Tue, 02 Aug 2022 15:23:16: 13000000 INFO @ Tue, 02 Aug 2022 15:23:18: 18000000 INFO @ Tue, 02 Aug 2022 15:23:23: 23000000 INFO @ Tue, 02 Aug 2022 15:23:26: 14000000 INFO @ Tue, 02 Aug 2022 15:23:27: 19000000 INFO @ Tue, 02 Aug 2022 15:23:32: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:23:35: 15000000 INFO @ Tue, 02 Aug 2022 15:23:36: 20000000 INFO @ Tue, 02 Aug 2022 15:23:40: 25000000 INFO @ Tue, 02 Aug 2022 15:23:44: 21000000 INFO @ Tue, 02 Aug 2022 15:23:44: 16000000 INFO @ Tue, 02 Aug 2022 15:23:49: 26000000 INFO @ Tue, 02 Aug 2022 15:23:53: 22000000 INFO @ Tue, 02 Aug 2022 15:23:54: 17000000 INFO @ Tue, 02 Aug 2022 15:23:58: 27000000 INFO @ Tue, 02 Aug 2022 15:24:02: 23000000 INFO @ Tue, 02 Aug 2022 15:24:03: 18000000 INFO @ Tue, 02 Aug 2022 15:24:07: 28000000 INFO @ Tue, 02 Aug 2022 15:24:11: 24000000 INFO @ Tue, 02 Aug 2022 15:24:12: 19000000 INFO @ Tue, 02 Aug 2022 15:24:15: 29000000 INFO @ Tue, 02 Aug 2022 15:24:19: 25000000 INFO @ Tue, 02 Aug 2022 15:24:22: 20000000 INFO @ Tue, 02 Aug 2022 15:24:24: 30000000 INFO @ Tue, 02 Aug 2022 15:24:28: 26000000 INFO @ Tue, 02 Aug 2022 15:24:31: 21000000 INFO @ Tue, 02 Aug 2022 15:24:32: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:24:32: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:24:32: #1 total tags in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:24:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:24:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:24:32: #1 tags after filtering in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:24:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:24:32: #1 finished! INFO @ Tue, 02 Aug 2022 15:24:32: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:24:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:24:34: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:24:34: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:24:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:24:37: 27000000 INFO @ Tue, 02 Aug 2022 15:24:40: 22000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:24:46: 28000000 INFO @ Tue, 02 Aug 2022 15:24:50: 23000000 INFO @ Tue, 02 Aug 2022 15:24:55: 29000000 INFO @ Tue, 02 Aug 2022 15:24:59: 24000000 INFO @ Tue, 02 Aug 2022 15:25:04: 30000000 INFO @ Tue, 02 Aug 2022 15:25:09: 25000000 INFO @ Tue, 02 Aug 2022 15:25:12: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:25:12: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:25:12: #1 total tags in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:25:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:25:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:25:13: #1 tags after filtering in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:25:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:25:13: #1 finished! INFO @ Tue, 02 Aug 2022 15:25:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:25:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:25:15: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:25:15: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:25:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:25:18: 26000000 INFO @ Tue, 02 Aug 2022 15:25:26: 27000000 INFO @ Tue, 02 Aug 2022 15:25:35: 28000000 INFO @ Tue, 02 Aug 2022 15:25:43: 29000000 INFO @ Tue, 02 Aug 2022 15:25:51: 30000000 INFO @ Tue, 02 Aug 2022 15:25:59: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:25:59: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:25:59: #1 total tags in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:25:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:25:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:25:59: #1 tags after filtering in treatment: 30895884 INFO @ Tue, 02 Aug 2022 15:25:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:25:59: #1 finished! INFO @ Tue, 02 Aug 2022 15:25:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:25:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:26:01: #2 number of paired peaks: 4 WARNING @ Tue, 02 Aug 2022 15:26:01: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:26:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX13227887/SRX13227887.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling