Job ID = 6527603 SRX = SRX1300696 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:06:54 prefetch.2.10.7: 1) Downloading 'SRR2548372'... 2020-06-29T13:06:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:10:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:10:02 prefetch.2.10.7: 1) 'SRR2548372' was downloaded successfully Read 24483693 spots for SRR2548372/SRR2548372.sra Written 24483693 spots for SRR2548372/SRR2548372.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:03 24483693 reads; of these: 24483693 (100.00%) were unpaired; of these: 1704446 (6.96%) aligned 0 times 16328657 (66.69%) aligned exactly 1 time 6450590 (26.35%) aligned >1 times 93.04% overall alignment rate Time searching: 00:08:03 Overall time: 00:08:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2571297 / 22779247 = 0.1129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:30:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:30:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:30:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:30:44: 1000000 INFO @ Mon, 29 Jun 2020 22:30:52: 2000000 INFO @ Mon, 29 Jun 2020 22:30:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:31:06: 4000000 INFO @ Mon, 29 Jun 2020 22:31:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:31:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:31:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:31:14: 1000000 INFO @ Mon, 29 Jun 2020 22:31:15: 5000000 INFO @ Mon, 29 Jun 2020 22:31:21: 2000000 INFO @ Mon, 29 Jun 2020 22:31:23: 6000000 INFO @ Mon, 29 Jun 2020 22:31:29: 3000000 INFO @ Mon, 29 Jun 2020 22:31:31: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:31:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:31:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:31:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:31:36: 4000000 INFO @ Mon, 29 Jun 2020 22:31:40: 8000000 INFO @ Mon, 29 Jun 2020 22:31:44: 5000000 INFO @ Mon, 29 Jun 2020 22:31:45: 1000000 INFO @ Mon, 29 Jun 2020 22:31:48: 9000000 INFO @ Mon, 29 Jun 2020 22:31:52: 6000000 INFO @ Mon, 29 Jun 2020 22:31:53: 2000000 INFO @ Mon, 29 Jun 2020 22:31:56: 10000000 INFO @ Mon, 29 Jun 2020 22:31:59: 7000000 INFO @ Mon, 29 Jun 2020 22:32:01: 3000000 INFO @ Mon, 29 Jun 2020 22:32:05: 11000000 INFO @ Mon, 29 Jun 2020 22:32:07: 8000000 INFO @ Mon, 29 Jun 2020 22:32:10: 4000000 INFO @ Mon, 29 Jun 2020 22:32:13: 12000000 INFO @ Mon, 29 Jun 2020 22:32:14: 9000000 INFO @ Mon, 29 Jun 2020 22:32:18: 5000000 INFO @ Mon, 29 Jun 2020 22:32:21: 13000000 INFO @ Mon, 29 Jun 2020 22:32:22: 10000000 INFO @ Mon, 29 Jun 2020 22:32:26: 6000000 INFO @ Mon, 29 Jun 2020 22:32:29: 14000000 INFO @ Mon, 29 Jun 2020 22:32:29: 11000000 INFO @ Mon, 29 Jun 2020 22:32:34: 7000000 INFO @ Mon, 29 Jun 2020 22:32:37: 12000000 INFO @ Mon, 29 Jun 2020 22:32:38: 15000000 INFO @ Mon, 29 Jun 2020 22:32:43: 8000000 INFO @ Mon, 29 Jun 2020 22:32:44: 13000000 INFO @ Mon, 29 Jun 2020 22:32:46: 16000000 INFO @ Mon, 29 Jun 2020 22:32:51: 9000000 INFO @ Mon, 29 Jun 2020 22:32:52: 14000000 INFO @ Mon, 29 Jun 2020 22:32:55: 17000000 INFO @ Mon, 29 Jun 2020 22:32:59: 10000000 INFO @ Mon, 29 Jun 2020 22:33:00: 15000000 INFO @ Mon, 29 Jun 2020 22:33:03: 18000000 INFO @ Mon, 29 Jun 2020 22:33:07: 16000000 INFO @ Mon, 29 Jun 2020 22:33:08: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:33:11: 19000000 INFO @ Mon, 29 Jun 2020 22:33:15: 17000000 INFO @ Mon, 29 Jun 2020 22:33:16: 12000000 INFO @ Mon, 29 Jun 2020 22:33:20: 20000000 INFO @ Mon, 29 Jun 2020 22:33:22: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:33:22: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:33:22: #1 total tags in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:33:22: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:33:22: #1 tags after filtering in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:33:22: #1 finished! INFO @ Mon, 29 Jun 2020 22:33:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:33:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:33:22: 18000000 INFO @ Mon, 29 Jun 2020 22:33:23: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 22:33:23: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:33:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:33:25: 13000000 INFO @ Mon, 29 Jun 2020 22:33:30: 19000000 INFO @ Mon, 29 Jun 2020 22:33:33: 14000000 INFO @ Mon, 29 Jun 2020 22:33:37: 20000000 INFO @ Mon, 29 Jun 2020 22:33:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:33:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:33:39: #1 total tags in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:33:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:33:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:33:39: #1 tags after filtering in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:33:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:33:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:33:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:33:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:33:41: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 22:33:41: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:33:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:33:41: 15000000 INFO @ Mon, 29 Jun 2020 22:33:49: 16000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 22:33:56: 17000000 INFO @ Mon, 29 Jun 2020 22:34:03: 18000000 INFO @ Mon, 29 Jun 2020 22:34:10: 19000000 INFO @ Mon, 29 Jun 2020 22:34:18: 20000000 INFO @ Mon, 29 Jun 2020 22:34:19: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:34:19: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:34:19: #1 total tags in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:34:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:34:20: #1 tags after filtering in treatment: 20207950 INFO @ Mon, 29 Jun 2020 22:34:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:34:20: #1 finished! INFO @ Mon, 29 Jun 2020 22:34:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:34:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:34:21: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 22:34:21: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:34:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1300696/SRX1300696.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling