Job ID = 9158436 sra ファイルのダウンロード中... Completed: 946479K bytes transferred in 10 seconds (746847K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 26825146 spots for /home/okishinya/chipatlas/results/dm3/SRX1300695/SRR2548371.sra Written 26825146 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:38 26825146 reads; of these: 26825146 (100.00%) were unpaired; of these: 1621589 (6.05%) aligned 0 times 17835229 (66.49%) aligned exactly 1 time 7368328 (27.47%) aligned >1 times 93.95% overall alignment rate Time searching: 00:10:38 Overall time: 00:10:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3344867 / 25203557 = 0.1327 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:03:14: # Command line: callpeak -t SRX1300695.bam -f BAM -g dm -n SRX1300695.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1300695.10 # format = BAM # ChIP-seq file = ['SRX1300695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:03:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:03:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:03:14: # Command line: callpeak -t SRX1300695.bam -f BAM -g dm -n SRX1300695.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1300695.05 # format = BAM # ChIP-seq file = ['SRX1300695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:03:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:03:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:03:14: # Command line: callpeak -t SRX1300695.bam -f BAM -g dm -n SRX1300695.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1300695.20 # format = BAM # ChIP-seq file = ['SRX1300695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:03:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:03:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:03:22: 1000000 INFO @ Tue, 27 Jun 2017 17:03:23: 1000000 INFO @ Tue, 27 Jun 2017 17:03:23: 1000000 INFO @ Tue, 27 Jun 2017 17:03:29: 2000000 INFO @ Tue, 27 Jun 2017 17:03:31: 2000000 INFO @ Tue, 27 Jun 2017 17:03:31: 2000000 INFO @ Tue, 27 Jun 2017 17:03:37: 3000000 INFO @ Tue, 27 Jun 2017 17:03:39: 3000000 INFO @ Tue, 27 Jun 2017 17:03:40: 3000000 INFO @ Tue, 27 Jun 2017 17:03:44: 4000000 INFO @ Tue, 27 Jun 2017 17:03:48: 4000000 INFO @ Tue, 27 Jun 2017 17:03:48: 4000000 INFO @ Tue, 27 Jun 2017 17:03:51: 5000000 INFO @ Tue, 27 Jun 2017 17:03:56: 5000000 INFO @ Tue, 27 Jun 2017 17:03:56: 5000000 INFO @ Tue, 27 Jun 2017 17:03:59: 6000000 INFO @ Tue, 27 Jun 2017 17:04:04: 6000000 INFO @ Tue, 27 Jun 2017 17:04:05: 6000000 INFO @ Tue, 27 Jun 2017 17:04:06: 7000000 INFO @ Tue, 27 Jun 2017 17:04:11: 7000000 INFO @ Tue, 27 Jun 2017 17:04:13: 7000000 INFO @ Tue, 27 Jun 2017 17:04:14: 8000000 INFO @ Tue, 27 Jun 2017 17:04:19: 8000000 INFO @ Tue, 27 Jun 2017 17:04:22: 8000000 INFO @ Tue, 27 Jun 2017 17:04:22: 9000000 INFO @ Tue, 27 Jun 2017 17:04:27: 9000000 INFO @ Tue, 27 Jun 2017 17:04:30: 10000000 INFO @ Tue, 27 Jun 2017 17:04:31: 9000000 INFO @ Tue, 27 Jun 2017 17:04:34: 10000000 INFO @ Tue, 27 Jun 2017 17:04:38: 11000000 INFO @ Tue, 27 Jun 2017 17:04:39: 10000000 INFO @ Tue, 27 Jun 2017 17:04:42: 11000000 INFO @ Tue, 27 Jun 2017 17:04:46: 12000000 INFO @ Tue, 27 Jun 2017 17:04:48: 11000000 INFO @ Tue, 27 Jun 2017 17:04:49: 12000000 INFO @ Tue, 27 Jun 2017 17:04:54: 13000000 INFO @ Tue, 27 Jun 2017 17:04:57: 12000000 INFO @ Tue, 27 Jun 2017 17:04:57: 13000000 INFO @ Tue, 27 Jun 2017 17:05:02: 14000000 INFO @ Tue, 27 Jun 2017 17:05:04: 14000000 INFO @ Tue, 27 Jun 2017 17:05:05: 13000000 INFO @ Tue, 27 Jun 2017 17:05:09: 15000000 INFO @ Tue, 27 Jun 2017 17:05:12: 15000000 INFO @ Tue, 27 Jun 2017 17:05:14: 14000000 INFO @ Tue, 27 Jun 2017 17:05:17: 16000000 INFO @ Tue, 27 Jun 2017 17:05:19: 16000000 INFO @ Tue, 27 Jun 2017 17:05:23: 15000000 INFO @ Tue, 27 Jun 2017 17:05:25: 17000000 INFO @ Tue, 27 Jun 2017 17:05:27: 17000000 INFO @ Tue, 27 Jun 2017 17:05:32: 16000000 INFO @ Tue, 27 Jun 2017 17:05:33: 18000000 INFO @ Tue, 27 Jun 2017 17:05:35: 18000000 INFO @ Tue, 27 Jun 2017 17:05:40: 17000000 INFO @ Tue, 27 Jun 2017 17:05:41: 19000000 INFO @ Tue, 27 Jun 2017 17:05:42: 19000000 INFO @ Tue, 27 Jun 2017 17:05:49: 18000000 INFO @ Tue, 27 Jun 2017 17:05:49: 20000000 INFO @ Tue, 27 Jun 2017 17:05:50: 20000000 INFO @ Tue, 27 Jun 2017 17:05:57: 21000000 INFO @ Tue, 27 Jun 2017 17:05:58: 21000000 INFO @ Tue, 27 Jun 2017 17:05:58: 19000000 INFO @ Tue, 27 Jun 2017 17:06:05: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:06:05: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:06:05: #1 total tags in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:05: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:06:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:06:05: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:06:05: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:06:05: #1 total tags in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:05: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:06:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:06:05: #1 tags after filtering in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:06:05: #1 finished! INFO @ Tue, 27 Jun 2017 17:06:05: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:06:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:06:05: #1 tags after filtering in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:06:05: #1 finished! INFO @ Tue, 27 Jun 2017 17:06:05: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:06:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:06:07: #2 number of paired peaks: 65 WARNING @ Tue, 27 Jun 2017 17:06:07: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:06:07: Process for pairing-model is terminated! cat: SRX1300695.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300695.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:06:07: 20000000 INFO @ Tue, 27 Jun 2017 17:06:07: #2 number of paired peaks: 65 WARNING @ Tue, 27 Jun 2017 17:06:07: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:06:07: Process for pairing-model is terminated! cat: SRX1300695.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300695.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:06:15: 21000000 INFO @ Tue, 27 Jun 2017 17:06:21: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 17:06:21: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 17:06:21: #1 total tags in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:21: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:06:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:06:21: #1 tags after filtering in treatment: 21858690 INFO @ Tue, 27 Jun 2017 17:06:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:06:21: #1 finished! INFO @ Tue, 27 Jun 2017 17:06:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:06:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:06:23: #2 number of paired peaks: 65 WARNING @ Tue, 27 Jun 2017 17:06:23: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:06:23: Process for pairing-model is terminated! cat: SRX1300695.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1300695.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1300695.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。