Job ID = 1293828 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T17:48:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T17:48:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T17:48:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:02:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:02:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:02:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T18:06:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 51,342,224 reads read : 102,684,448 reads written : 102,684,448 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:16:08 51342224 reads; of these: 51342224 (100.00%) were paired; of these: 17637296 (34.35%) aligned concordantly 0 times 28214549 (54.95%) aligned concordantly exactly 1 time 5490379 (10.69%) aligned concordantly >1 times ---- 17637296 pairs aligned concordantly 0 times; of these: 60463 (0.34%) aligned discordantly 1 time ---- 17576833 pairs aligned 0 times concordantly or discordantly; of these: 35153666 mates make up the pairs; of these: 33589979 (95.55%) aligned 0 times 1215787 (3.46%) aligned exactly 1 time 347900 (0.99%) aligned >1 times 67.29% overall alignment rate Time searching: 01:16:08 Overall time: 01:16:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 12228207 / 33746844 = 0.3624 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:06:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:06:05: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:06:05: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:06:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:06:05: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:06:05: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:06:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:06:05: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:06:05: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:06:12: 1000000 INFO @ Mon, 03 Jun 2019 05:06:15: 1000000 INFO @ Mon, 03 Jun 2019 05:06:16: 1000000 INFO @ Mon, 03 Jun 2019 05:06:20: 2000000 INFO @ Mon, 03 Jun 2019 05:06:24: 2000000 INFO @ Mon, 03 Jun 2019 05:06:25: 2000000 INFO @ Mon, 03 Jun 2019 05:06:27: 3000000 INFO @ Mon, 03 Jun 2019 05:06:33: 3000000 INFO @ Mon, 03 Jun 2019 05:06:34: 4000000 INFO @ Mon, 03 Jun 2019 05:06:35: 3000000 INFO @ Mon, 03 Jun 2019 05:06:42: 5000000 INFO @ Mon, 03 Jun 2019 05:06:43: 4000000 INFO @ Mon, 03 Jun 2019 05:06:45: 4000000 INFO @ Mon, 03 Jun 2019 05:06:50: 6000000 INFO @ Mon, 03 Jun 2019 05:06:54: 5000000 INFO @ Mon, 03 Jun 2019 05:06:55: 5000000 INFO @ Mon, 03 Jun 2019 05:06:59: 7000000 INFO @ Mon, 03 Jun 2019 05:07:03: 6000000 INFO @ Mon, 03 Jun 2019 05:07:05: 6000000 INFO @ Mon, 03 Jun 2019 05:07:06: 8000000 INFO @ Mon, 03 Jun 2019 05:07:13: 7000000 INFO @ Mon, 03 Jun 2019 05:07:14: 9000000 INFO @ Mon, 03 Jun 2019 05:07:15: 7000000 INFO @ Mon, 03 Jun 2019 05:07:22: 10000000 INFO @ Mon, 03 Jun 2019 05:07:22: 8000000 INFO @ Mon, 03 Jun 2019 05:07:24: 8000000 INFO @ Mon, 03 Jun 2019 05:07:30: 11000000 INFO @ Mon, 03 Jun 2019 05:07:32: 9000000 INFO @ Mon, 03 Jun 2019 05:07:34: 9000000 INFO @ Mon, 03 Jun 2019 05:07:37: 12000000 INFO @ Mon, 03 Jun 2019 05:07:42: 10000000 INFO @ Mon, 03 Jun 2019 05:07:44: 10000000 INFO @ Mon, 03 Jun 2019 05:07:45: 13000000 INFO @ Mon, 03 Jun 2019 05:07:51: 11000000 INFO @ Mon, 03 Jun 2019 05:07:52: 14000000 INFO @ Mon, 03 Jun 2019 05:07:54: 11000000 INFO @ Mon, 03 Jun 2019 05:08:00: 15000000 INFO @ Mon, 03 Jun 2019 05:08:00: 12000000 INFO @ Mon, 03 Jun 2019 05:08:03: 12000000 INFO @ Mon, 03 Jun 2019 05:08:07: 16000000 INFO @ Mon, 03 Jun 2019 05:08:11: 13000000 INFO @ Mon, 03 Jun 2019 05:08:13: 13000000 INFO @ Mon, 03 Jun 2019 05:08:15: 17000000 INFO @ Mon, 03 Jun 2019 05:08:21: 14000000 INFO @ Mon, 03 Jun 2019 05:08:22: 18000000 INFO @ Mon, 03 Jun 2019 05:08:23: 14000000 INFO @ Mon, 03 Jun 2019 05:08:29: 19000000 INFO @ Mon, 03 Jun 2019 05:08:30: 15000000 INFO @ Mon, 03 Jun 2019 05:08:33: 15000000 INFO @ Mon, 03 Jun 2019 05:08:36: 20000000 INFO @ Mon, 03 Jun 2019 05:08:39: 16000000 INFO @ Mon, 03 Jun 2019 05:08:42: 16000000 INFO @ Mon, 03 Jun 2019 05:08:43: 21000000 INFO @ Mon, 03 Jun 2019 05:08:48: 17000000 INFO @ Mon, 03 Jun 2019 05:08:50: 22000000 INFO @ Mon, 03 Jun 2019 05:08:51: 17000000 INFO @ Mon, 03 Jun 2019 05:08:56: 18000000 INFO @ Mon, 03 Jun 2019 05:08:57: 23000000 INFO @ Mon, 03 Jun 2019 05:09:00: 18000000 INFO @ Mon, 03 Jun 2019 05:09:05: 24000000 INFO @ Mon, 03 Jun 2019 05:09:05: 19000000 INFO @ Mon, 03 Jun 2019 05:09:10: 19000000 INFO @ Mon, 03 Jun 2019 05:09:12: 25000000 INFO @ Mon, 03 Jun 2019 05:09:13: 20000000 INFO @ Mon, 03 Jun 2019 05:09:19: 26000000 INFO @ Mon, 03 Jun 2019 05:09:19: 20000000 INFO @ Mon, 03 Jun 2019 05:09:22: 21000000 INFO @ Mon, 03 Jun 2019 05:09:26: 27000000 INFO @ Mon, 03 Jun 2019 05:09:28: 21000000 INFO @ Mon, 03 Jun 2019 05:09:30: 22000000 INFO @ Mon, 03 Jun 2019 05:09:33: 28000000 INFO @ Mon, 03 Jun 2019 05:09:38: 22000000 INFO @ Mon, 03 Jun 2019 05:09:39: 23000000 INFO @ Mon, 03 Jun 2019 05:09:42: 29000000 INFO @ Mon, 03 Jun 2019 05:09:48: 23000000 INFO @ Mon, 03 Jun 2019 05:09:48: 24000000 INFO @ Mon, 03 Jun 2019 05:09:49: 30000000 INFO @ Mon, 03 Jun 2019 05:09:56: 31000000 INFO @ Mon, 03 Jun 2019 05:09:57: 24000000 INFO @ Mon, 03 Jun 2019 05:09:57: 25000000 INFO @ Mon, 03 Jun 2019 05:10:03: 32000000 INFO @ Mon, 03 Jun 2019 05:10:05: 26000000 INFO @ Mon, 03 Jun 2019 05:10:06: 25000000 INFO @ Mon, 03 Jun 2019 05:10:10: 33000000 INFO @ Mon, 03 Jun 2019 05:10:13: 27000000 INFO @ Mon, 03 Jun 2019 05:10:15: 26000000 INFO @ Mon, 03 Jun 2019 05:10:17: 34000000 INFO @ Mon, 03 Jun 2019 05:10:23: 28000000 INFO @ Mon, 03 Jun 2019 05:10:25: 27000000 INFO @ Mon, 03 Jun 2019 05:10:25: 35000000 INFO @ Mon, 03 Jun 2019 05:10:31: 29000000 INFO @ Mon, 03 Jun 2019 05:10:32: 36000000 INFO @ Mon, 03 Jun 2019 05:10:34: 28000000 INFO @ Mon, 03 Jun 2019 05:10:39: 37000000 INFO @ Mon, 03 Jun 2019 05:10:40: 30000000 INFO @ Mon, 03 Jun 2019 05:10:43: 29000000 INFO @ Mon, 03 Jun 2019 05:10:46: 38000000 INFO @ Mon, 03 Jun 2019 05:10:49: 31000000 INFO @ Mon, 03 Jun 2019 05:10:52: 30000000 INFO @ Mon, 03 Jun 2019 05:10:53: 39000000 INFO @ Mon, 03 Jun 2019 05:10:59: 32000000 INFO @ Mon, 03 Jun 2019 05:11:01: 40000000 INFO @ Mon, 03 Jun 2019 05:11:02: 31000000 INFO @ Mon, 03 Jun 2019 05:11:08: 33000000 INFO @ Mon, 03 Jun 2019 05:11:08: 41000000 INFO @ Mon, 03 Jun 2019 05:11:11: 32000000 INFO @ Mon, 03 Jun 2019 05:11:15: 42000000 INFO @ Mon, 03 Jun 2019 05:11:16: 34000000 INFO @ Mon, 03 Jun 2019 05:11:21: 33000000 INFO @ Mon, 03 Jun 2019 05:11:22: 43000000 INFO @ Mon, 03 Jun 2019 05:11:25: 35000000 INFO @ Mon, 03 Jun 2019 05:11:29: 44000000 INFO @ Mon, 03 Jun 2019 05:11:30: 34000000 INFO @ Mon, 03 Jun 2019 05:11:33: 36000000 INFO @ Mon, 03 Jun 2019 05:11:34: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 05:11:34: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 05:11:34: #1 total tags in treatment: 21485730 INFO @ Mon, 03 Jun 2019 05:11:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:11:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:11:34: #1 tags after filtering in treatment: 16248947 INFO @ Mon, 03 Jun 2019 05:11:34: #1 Redundant rate of treatment: 0.24 INFO @ Mon, 03 Jun 2019 05:11:34: #1 finished! INFO @ Mon, 03 Jun 2019 05:11:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:11:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:11:36: #2 number of paired peaks: 4315 INFO @ Mon, 03 Jun 2019 05:11:36: start model_add_line... INFO @ Mon, 03 Jun 2019 05:11:37: start X-correlation... INFO @ Mon, 03 Jun 2019 05:11:37: end of X-cor INFO @ Mon, 03 Jun 2019 05:11:37: #2 finished! INFO @ Mon, 03 Jun 2019 05:11:37: #2 predicted fragment length is 145 bps INFO @ Mon, 03 Jun 2019 05:11:37: #2 alternative fragment length(s) may be 145 bps INFO @ Mon, 03 Jun 2019 05:11:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20_model.r INFO @ Mon, 03 Jun 2019 05:11:37: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:11:37: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:11:39: 35000000 INFO @ Mon, 03 Jun 2019 05:11:41: 37000000 INFO @ Mon, 03 Jun 2019 05:11:48: 36000000 INFO @ Mon, 03 Jun 2019 05:11:49: 38000000 INFO @ Mon, 03 Jun 2019 05:11:57: 39000000 INFO @ Mon, 03 Jun 2019 05:11:57: 37000000 INFO @ Mon, 03 Jun 2019 05:12:06: 40000000 INFO @ Mon, 03 Jun 2019 05:12:07: 38000000 INFO @ Mon, 03 Jun 2019 05:12:14: 41000000 INFO @ Mon, 03 Jun 2019 05:12:16: 39000000 INFO @ Mon, 03 Jun 2019 05:12:22: 42000000 INFO @ Mon, 03 Jun 2019 05:12:25: 40000000 INFO @ Mon, 03 Jun 2019 05:12:30: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:12:30: 43000000 INFO @ Mon, 03 Jun 2019 05:12:34: 41000000 INFO @ Mon, 03 Jun 2019 05:12:39: 44000000 INFO @ Mon, 03 Jun 2019 05:12:43: 42000000 INFO @ Mon, 03 Jun 2019 05:12:44: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 05:12:44: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 05:12:44: #1 total tags in treatment: 21485730 INFO @ Mon, 03 Jun 2019 05:12:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:12:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:12:44: #1 tags after filtering in treatment: 16248947 INFO @ Mon, 03 Jun 2019 05:12:44: #1 Redundant rate of treatment: 0.24 INFO @ Mon, 03 Jun 2019 05:12:44: #1 finished! INFO @ Mon, 03 Jun 2019 05:12:44: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:12:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:12:46: #2 number of paired peaks: 4315 INFO @ Mon, 03 Jun 2019 05:12:46: start model_add_line... INFO @ Mon, 03 Jun 2019 05:12:47: start X-correlation... INFO @ Mon, 03 Jun 2019 05:12:47: end of X-cor INFO @ Mon, 03 Jun 2019 05:12:47: #2 finished! INFO @ Mon, 03 Jun 2019 05:12:47: #2 predicted fragment length is 145 bps INFO @ Mon, 03 Jun 2019 05:12:47: #2 alternative fragment length(s) may be 145 bps INFO @ Mon, 03 Jun 2019 05:12:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05_model.r INFO @ Mon, 03 Jun 2019 05:12:47: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:12:47: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:12:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20_peaks.xls INFO @ Mon, 03 Jun 2019 05:12:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:12:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.20_summits.bed INFO @ Mon, 03 Jun 2019 05:12:51: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (4219 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:12:52: 43000000 INFO @ Mon, 03 Jun 2019 05:13:01: 44000000 INFO @ Mon, 03 Jun 2019 05:13:07: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 05:13:07: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 05:13:07: #1 total tags in treatment: 21485730 INFO @ Mon, 03 Jun 2019 05:13:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:13:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:13:07: #1 tags after filtering in treatment: 16248947 INFO @ Mon, 03 Jun 2019 05:13:07: #1 Redundant rate of treatment: 0.24 INFO @ Mon, 03 Jun 2019 05:13:07: #1 finished! INFO @ Mon, 03 Jun 2019 05:13:07: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:13:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:13:09: #2 number of paired peaks: 4315 INFO @ Mon, 03 Jun 2019 05:13:09: start model_add_line... INFO @ Mon, 03 Jun 2019 05:13:09: start X-correlation... INFO @ Mon, 03 Jun 2019 05:13:09: end of X-cor INFO @ Mon, 03 Jun 2019 05:13:09: #2 finished! INFO @ Mon, 03 Jun 2019 05:13:09: #2 predicted fragment length is 145 bps INFO @ Mon, 03 Jun 2019 05:13:09: #2 alternative fragment length(s) may be 145 bps INFO @ Mon, 03 Jun 2019 05:13:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10_model.r INFO @ Mon, 03 Jun 2019 05:13:09: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:13:09: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:13:42: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:14:02: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:14:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05_peaks.xls INFO @ Mon, 03 Jun 2019 05:14:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:14:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.05_summits.bed INFO @ Mon, 03 Jun 2019 05:14:06: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (9402 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:14:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10_peaks.xls INFO @ Mon, 03 Jun 2019 05:14:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:14:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX127435/SRX127435.10_summits.bed INFO @ Mon, 03 Jun 2019 05:14:23: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6537 records, 4 fields): 11 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。