Job ID = 6527595 SRX = SRX124476 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:04:24 prefetch.2.10.7: 1) Downloading 'SRR423932'... 2020-06-29T13:04:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:05:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:05:02 prefetch.2.10.7: 'SRR423932' is valid 2020-06-29T13:05:02 prefetch.2.10.7: 1) 'SRR423932' was downloaded successfully Read 7414356 spots for SRR423932/SRR423932.sra Written 7414356 spots for SRR423932/SRR423932.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:39 7414356 reads; of these: 7414356 (100.00%) were unpaired; of these: 227675 (3.07%) aligned 0 times 5628523 (75.91%) aligned exactly 1 time 1558158 (21.02%) aligned >1 times 96.93% overall alignment rate Time searching: 00:01:39 Overall time: 00:01:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 341956 / 7186681 = 0.0476 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:09:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:09:54: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:09:54: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:09:59: 1000000 INFO @ Mon, 29 Jun 2020 22:10:04: 2000000 INFO @ Mon, 29 Jun 2020 22:10:08: 3000000 INFO @ Mon, 29 Jun 2020 22:10:13: 4000000 INFO @ Mon, 29 Jun 2020 22:10:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:10:22: 6000000 INFO @ Mon, 29 Jun 2020 22:10:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:10:24: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:10:24: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:10:27: #1 tag size is determined as 30 bps INFO @ Mon, 29 Jun 2020 22:10:27: #1 tag size = 30 INFO @ Mon, 29 Jun 2020 22:10:27: #1 total tags in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:10:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:10:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:10:27: #1 tags after filtering in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:10:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:10:27: #1 finished! INFO @ Mon, 29 Jun 2020 22:10:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:10:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:10:27: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 22:10:27: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:10:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:10:29: 1000000 INFO @ Mon, 29 Jun 2020 22:10:34: 2000000 INFO @ Mon, 29 Jun 2020 22:10:39: 3000000 INFO @ Mon, 29 Jun 2020 22:10:43: 4000000 INFO @ Mon, 29 Jun 2020 22:10:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:10:53: 6000000 INFO @ Mon, 29 Jun 2020 22:10:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:10:54: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:10:54: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:10:57: #1 tag size is determined as 30 bps INFO @ Mon, 29 Jun 2020 22:10:57: #1 tag size = 30 INFO @ Mon, 29 Jun 2020 22:10:57: #1 total tags in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:10:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:10:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:10:57: #1 tags after filtering in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:10:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:10:57: #1 finished! INFO @ Mon, 29 Jun 2020 22:10:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:10:58: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 22:10:58: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:10:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:10:59: 1000000 INFO @ Mon, 29 Jun 2020 22:11:04: 2000000 INFO @ Mon, 29 Jun 2020 22:11:08: 3000000 INFO @ Mon, 29 Jun 2020 22:11:13: 4000000 INFO @ Mon, 29 Jun 2020 22:11:18: 5000000 INFO @ Mon, 29 Jun 2020 22:11:23: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:11:27: #1 tag size is determined as 30 bps INFO @ Mon, 29 Jun 2020 22:11:27: #1 tag size = 30 INFO @ Mon, 29 Jun 2020 22:11:27: #1 total tags in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:11:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:11:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:11:27: #1 tags after filtering in treatment: 6844725 INFO @ Mon, 29 Jun 2020 22:11:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:11:27: #1 finished! INFO @ Mon, 29 Jun 2020 22:11:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:11:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:11:28: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 22:11:28: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:11:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX124476/SRX124476.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。