Job ID = 16438142 SRX = SRX12261368 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 45218482 spots for SRR15972624/SRR15972624.sra Written 45218482 spots for SRR15972624/SRR15972624.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439170 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:50:31 45218482 reads; of these: 45218482 (100.00%) were paired; of these: 8117938 (17.95%) aligned concordantly 0 times 26381977 (58.34%) aligned concordantly exactly 1 time 10718567 (23.70%) aligned concordantly >1 times ---- 8117938 pairs aligned concordantly 0 times; of these: 158386 (1.95%) aligned discordantly 1 time ---- 7959552 pairs aligned 0 times concordantly or discordantly; of these: 15919104 mates make up the pairs; of these: 14839892 (93.22%) aligned 0 times 602653 (3.79%) aligned exactly 1 time 476559 (2.99%) aligned >1 times 83.59% overall alignment rate Time searching: 00:50:32 Overall time: 00:50:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8810370 / 24721267 = 0.3564 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:58:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:58:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:58:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:58:39: 1000000 INFO @ Tue, 02 Aug 2022 14:58:46: 2000000 INFO @ Tue, 02 Aug 2022 14:58:53: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:59:00: 4000000 INFO @ Tue, 02 Aug 2022 14:59:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:59:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:59:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:59:07: 5000000 INFO @ Tue, 02 Aug 2022 14:59:08: 1000000 INFO @ Tue, 02 Aug 2022 14:59:14: 6000000 INFO @ Tue, 02 Aug 2022 14:59:16: 2000000 INFO @ Tue, 02 Aug 2022 14:59:20: 7000000 INFO @ Tue, 02 Aug 2022 14:59:23: 3000000 INFO @ Tue, 02 Aug 2022 14:59:27: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:59:31: 4000000 INFO @ Tue, 02 Aug 2022 14:59:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:59:31: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:59:31: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:59:33: 9000000 INFO @ Tue, 02 Aug 2022 14:59:38: 5000000 INFO @ Tue, 02 Aug 2022 14:59:38: 1000000 INFO @ Tue, 02 Aug 2022 14:59:39: 10000000 INFO @ Tue, 02 Aug 2022 14:59:44: 6000000 INFO @ Tue, 02 Aug 2022 14:59:46: 11000000 INFO @ Tue, 02 Aug 2022 14:59:46: 2000000 INFO @ Tue, 02 Aug 2022 14:59:51: 7000000 INFO @ Tue, 02 Aug 2022 14:59:53: 12000000 INFO @ Tue, 02 Aug 2022 14:59:53: 3000000 INFO @ Tue, 02 Aug 2022 14:59:58: 8000000 INFO @ Tue, 02 Aug 2022 15:00:00: 13000000 INFO @ Tue, 02 Aug 2022 15:00:00: 4000000 INFO @ Tue, 02 Aug 2022 15:00:04: 9000000 INFO @ Tue, 02 Aug 2022 15:00:07: 14000000 INFO @ Tue, 02 Aug 2022 15:00:07: 5000000 INFO @ Tue, 02 Aug 2022 15:00:11: 10000000 INFO @ Tue, 02 Aug 2022 15:00:14: 15000000 INFO @ Tue, 02 Aug 2022 15:00:15: 6000000 INFO @ Tue, 02 Aug 2022 15:00:18: 11000000 INFO @ Tue, 02 Aug 2022 15:00:21: 16000000 INFO @ Tue, 02 Aug 2022 15:00:22: 7000000 INFO @ Tue, 02 Aug 2022 15:00:25: 12000000 INFO @ Tue, 02 Aug 2022 15:00:28: 17000000 INFO @ Tue, 02 Aug 2022 15:00:30: 8000000 INFO @ Tue, 02 Aug 2022 15:00:32: 13000000 INFO @ Tue, 02 Aug 2022 15:00:34: 18000000 INFO @ Tue, 02 Aug 2022 15:00:37: 9000000 INFO @ Tue, 02 Aug 2022 15:00:39: 14000000 INFO @ Tue, 02 Aug 2022 15:00:41: 19000000 INFO @ Tue, 02 Aug 2022 15:00:44: 10000000 INFO @ Tue, 02 Aug 2022 15:00:46: 15000000 INFO @ Tue, 02 Aug 2022 15:00:48: 20000000 INFO @ Tue, 02 Aug 2022 15:00:51: 11000000 INFO @ Tue, 02 Aug 2022 15:00:52: 16000000 INFO @ Tue, 02 Aug 2022 15:00:54: 21000000 INFO @ Tue, 02 Aug 2022 15:00:57: 12000000 INFO @ Tue, 02 Aug 2022 15:00:58: 17000000 INFO @ Tue, 02 Aug 2022 15:01:01: 22000000 INFO @ Tue, 02 Aug 2022 15:01:03: 13000000 INFO @ Tue, 02 Aug 2022 15:01:05: 18000000 INFO @ Tue, 02 Aug 2022 15:01:07: 23000000 INFO @ Tue, 02 Aug 2022 15:01:09: 14000000 INFO @ Tue, 02 Aug 2022 15:01:11: 19000000 INFO @ Tue, 02 Aug 2022 15:01:13: 24000000 INFO @ Tue, 02 Aug 2022 15:01:15: 15000000 INFO @ Tue, 02 Aug 2022 15:01:17: 20000000 INFO @ Tue, 02 Aug 2022 15:01:19: 25000000 INFO @ Tue, 02 Aug 2022 15:01:22: 16000000 INFO @ Tue, 02 Aug 2022 15:01:23: 21000000 INFO @ Tue, 02 Aug 2022 15:01:25: 26000000 INFO @ Tue, 02 Aug 2022 15:01:27: 17000000 INFO @ Tue, 02 Aug 2022 15:01:30: 22000000 INFO @ Tue, 02 Aug 2022 15:01:32: 27000000 INFO @ Tue, 02 Aug 2022 15:01:33: 18000000 INFO @ Tue, 02 Aug 2022 15:01:36: 23000000 INFO @ Tue, 02 Aug 2022 15:01:38: 28000000 INFO @ Tue, 02 Aug 2022 15:01:40: 19000000 INFO @ Tue, 02 Aug 2022 15:01:42: 24000000 INFO @ Tue, 02 Aug 2022 15:01:44: 29000000 INFO @ Tue, 02 Aug 2022 15:01:46: 20000000 INFO @ Tue, 02 Aug 2022 15:01:48: 25000000 INFO @ Tue, 02 Aug 2022 15:01:50: 30000000 INFO @ Tue, 02 Aug 2022 15:01:51: 21000000 INFO @ Tue, 02 Aug 2022 15:01:54: 26000000 INFO @ Tue, 02 Aug 2022 15:01:56: 31000000 INFO @ Tue, 02 Aug 2022 15:01:57: 22000000 INFO @ Tue, 02 Aug 2022 15:02:00: 27000000 INFO @ Tue, 02 Aug 2022 15:02:02: 32000000 INFO @ Tue, 02 Aug 2022 15:02:03: 23000000 INFO @ Tue, 02 Aug 2022 15:02:06: 28000000 INFO @ Tue, 02 Aug 2022 15:02:08: 33000000 INFO @ Tue, 02 Aug 2022 15:02:10: 24000000 INFO @ Tue, 02 Aug 2022 15:02:13: 29000000 INFO @ Tue, 02 Aug 2022 15:02:14: 34000000 INFO @ Tue, 02 Aug 2022 15:02:16: 25000000 INFO @ Tue, 02 Aug 2022 15:02:18: 30000000 INFO @ Tue, 02 Aug 2022 15:02:20: 35000000 INFO @ Tue, 02 Aug 2022 15:02:22: 26000000 INFO @ Tue, 02 Aug 2022 15:02:24: 31000000 INFO @ Tue, 02 Aug 2022 15:02:26: 36000000 INFO @ Tue, 02 Aug 2022 15:02:28: 27000000 INFO @ Tue, 02 Aug 2022 15:02:31: 32000000 INFO @ Tue, 02 Aug 2022 15:02:32: 37000000 INFO @ Tue, 02 Aug 2022 15:02:34: 28000000 INFO @ Tue, 02 Aug 2022 15:02:37: 33000000 INFO @ Tue, 02 Aug 2022 15:02:39: 38000000 INFO @ Tue, 02 Aug 2022 15:02:40: 29000000 INFO @ Tue, 02 Aug 2022 15:02:43: 34000000 INFO @ Tue, 02 Aug 2022 15:02:45: 39000000 INFO @ Tue, 02 Aug 2022 15:02:46: 30000000 INFO @ Tue, 02 Aug 2022 15:02:49: 35000000 INFO @ Tue, 02 Aug 2022 15:02:51: 40000000 INFO @ Tue, 02 Aug 2022 15:02:52: 31000000 INFO @ Tue, 02 Aug 2022 15:02:55: 36000000 INFO @ Tue, 02 Aug 2022 15:02:57: 41000000 INFO @ Tue, 02 Aug 2022 15:02:59: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:03:01: 37000000 INFO @ Tue, 02 Aug 2022 15:03:03: 42000000 INFO @ Tue, 02 Aug 2022 15:03:05: 33000000 INFO @ Tue, 02 Aug 2022 15:03:08: 38000000 INFO @ Tue, 02 Aug 2022 15:03:09: 43000000 INFO @ Tue, 02 Aug 2022 15:03:11: 34000000 INFO @ Tue, 02 Aug 2022 15:03:14: 39000000 INFO @ Tue, 02 Aug 2022 15:03:16: 44000000 INFO @ Tue, 02 Aug 2022 15:03:18: 35000000 INFO @ Tue, 02 Aug 2022 15:03:20: 40000000 INFO @ Tue, 02 Aug 2022 15:03:22: 45000000 INFO @ Tue, 02 Aug 2022 15:03:24: 36000000 INFO @ Tue, 02 Aug 2022 15:03:26: 41000000 INFO @ Tue, 02 Aug 2022 15:03:28: 46000000 INFO @ Tue, 02 Aug 2022 15:03:30: 37000000 INFO @ Tue, 02 Aug 2022 15:03:32: 42000000 INFO @ Tue, 02 Aug 2022 15:03:34: 47000000 INFO @ Tue, 02 Aug 2022 15:03:36: 38000000 INFO @ Tue, 02 Aug 2022 15:03:38: 43000000 INFO @ Tue, 02 Aug 2022 15:03:40: 48000000 INFO @ Tue, 02 Aug 2022 15:03:42: 39000000 INFO @ Tue, 02 Aug 2022 15:03:44: 44000000 INFO @ Tue, 02 Aug 2022 15:03:46: 49000000 INFO @ Tue, 02 Aug 2022 15:03:48: 40000000 INFO @ Tue, 02 Aug 2022 15:03:50: 45000000 INFO @ Tue, 02 Aug 2022 15:03:53: 50000000 INFO @ Tue, 02 Aug 2022 15:03:54: 41000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:03:56: 46000000 INFO @ Tue, 02 Aug 2022 15:03:59: 51000000 INFO @ Tue, 02 Aug 2022 15:04:00: 42000000 INFO @ Tue, 02 Aug 2022 15:04:02: 47000000 INFO @ Tue, 02 Aug 2022 15:04:05: 52000000 INFO @ Tue, 02 Aug 2022 15:04:06: 43000000 INFO @ Tue, 02 Aug 2022 15:04:09: 48000000 INFO @ Tue, 02 Aug 2022 15:04:11: 53000000 INFO @ Tue, 02 Aug 2022 15:04:12: 44000000 INFO @ Tue, 02 Aug 2022 15:04:15: 49000000 INFO @ Tue, 02 Aug 2022 15:04:18: 54000000 INFO @ Tue, 02 Aug 2022 15:04:18: 45000000 INFO @ Tue, 02 Aug 2022 15:04:21: 50000000 INFO @ Tue, 02 Aug 2022 15:04:24: 55000000 INFO @ Tue, 02 Aug 2022 15:04:24: 46000000 INFO @ Tue, 02 Aug 2022 15:04:28: 51000000 INFO @ Tue, 02 Aug 2022 15:04:30: 56000000 INFO @ Tue, 02 Aug 2022 15:04:31: 47000000 INFO @ Tue, 02 Aug 2022 15:04:34: 52000000 INFO @ Tue, 02 Aug 2022 15:04:36: 57000000 INFO @ Tue, 02 Aug 2022 15:04:37: 48000000 INFO @ Tue, 02 Aug 2022 15:04:40: 53000000 INFO @ Tue, 02 Aug 2022 15:04:42: #1 tag size is determined as 66 bps INFO @ Tue, 02 Aug 2022 15:04:42: #1 tag size = 66 INFO @ Tue, 02 Aug 2022 15:04:42: #1 total tags in treatment: 28294801 INFO @ Tue, 02 Aug 2022 15:04:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:04:43: #1 tags after filtering in treatment: 15730506 INFO @ Tue, 02 Aug 2022 15:04:43: #1 Redundant rate of treatment: 0.44 INFO @ Tue, 02 Aug 2022 15:04:43: #1 finished! INFO @ Tue, 02 Aug 2022 15:04:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:04:44: 49000000 INFO @ Tue, 02 Aug 2022 15:04:44: #2 number of paired peaks: 886 WARNING @ Tue, 02 Aug 2022 15:04:44: Fewer paired peaks (886) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 886 pairs to build model! INFO @ Tue, 02 Aug 2022 15:04:44: start model_add_line... INFO @ Tue, 02 Aug 2022 15:04:44: start X-correlation... INFO @ Tue, 02 Aug 2022 15:04:44: end of X-cor INFO @ Tue, 02 Aug 2022 15:04:44: #2 finished! INFO @ Tue, 02 Aug 2022 15:04:44: #2 predicted fragment length is 61 bps INFO @ Tue, 02 Aug 2022 15:04:44: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 02 Aug 2022 15:04:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05_model.r WARNING @ Tue, 02 Aug 2022 15:04:45: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:04:45: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 02 Aug 2022 15:04:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:04:45: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:04:45: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:04:46: 54000000 INFO @ Tue, 02 Aug 2022 15:04:50: 50000000 INFO @ Tue, 02 Aug 2022 15:04:52: 55000000 INFO @ Tue, 02 Aug 2022 15:04:56: 51000000 INFO @ Tue, 02 Aug 2022 15:04:58: 56000000 INFO @ Tue, 02 Aug 2022 15:05:03: 52000000 INFO @ Tue, 02 Aug 2022 15:05:04: 57000000 INFO @ Tue, 02 Aug 2022 15:05:09: 53000000 INFO @ Tue, 02 Aug 2022 15:05:10: #1 tag size is determined as 66 bps INFO @ Tue, 02 Aug 2022 15:05:10: #1 tag size = 66 INFO @ Tue, 02 Aug 2022 15:05:10: #1 total tags in treatment: 28294801 INFO @ Tue, 02 Aug 2022 15:05:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:11: #1 tags after filtering in treatment: 15730506 INFO @ Tue, 02 Aug 2022 15:05:11: #1 Redundant rate of treatment: 0.44 INFO @ Tue, 02 Aug 2022 15:05:11: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:12: #2 number of paired peaks: 886 WARNING @ Tue, 02 Aug 2022 15:05:12: Fewer paired peaks (886) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 886 pairs to build model! INFO @ Tue, 02 Aug 2022 15:05:12: start model_add_line... INFO @ Tue, 02 Aug 2022 15:05:12: start X-correlation... INFO @ Tue, 02 Aug 2022 15:05:12: end of X-cor INFO @ Tue, 02 Aug 2022 15:05:12: #2 finished! INFO @ Tue, 02 Aug 2022 15:05:12: #2 predicted fragment length is 61 bps INFO @ Tue, 02 Aug 2022 15:05:12: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 02 Aug 2022 15:05:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10_model.r WARNING @ Tue, 02 Aug 2022 15:05:12: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:05:12: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 02 Aug 2022 15:05:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:05:12: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:05:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:05:14: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:05:15: 54000000 INFO @ Tue, 02 Aug 2022 15:05:21: 55000000 INFO @ Tue, 02 Aug 2022 15:05:27: 56000000 INFO @ Tue, 02 Aug 2022 15:05:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05_peaks.xls INFO @ Tue, 02 Aug 2022 15:05:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:05:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.05_summits.bed INFO @ Tue, 02 Aug 2022 15:05:32: Done! INFO @ Tue, 02 Aug 2022 15:05:32: 57000000 pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (19993 records, 4 fields): 47 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:05:38: #1 tag size is determined as 66 bps INFO @ Tue, 02 Aug 2022 15:05:38: #1 tag size = 66 INFO @ Tue, 02 Aug 2022 15:05:38: #1 total tags in treatment: 28294801 INFO @ Tue, 02 Aug 2022 15:05:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:39: #1 tags after filtering in treatment: 15730506 INFO @ Tue, 02 Aug 2022 15:05:39: #1 Redundant rate of treatment: 0.44 INFO @ Tue, 02 Aug 2022 15:05:39: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:40: #2 number of paired peaks: 886 WARNING @ Tue, 02 Aug 2022 15:05:40: Fewer paired peaks (886) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 886 pairs to build model! INFO @ Tue, 02 Aug 2022 15:05:40: start model_add_line... INFO @ Tue, 02 Aug 2022 15:05:40: start X-correlation... INFO @ Tue, 02 Aug 2022 15:05:40: end of X-cor INFO @ Tue, 02 Aug 2022 15:05:40: #2 finished! INFO @ Tue, 02 Aug 2022 15:05:40: #2 predicted fragment length is 61 bps INFO @ Tue, 02 Aug 2022 15:05:40: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 02 Aug 2022 15:05:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20_model.r WARNING @ Tue, 02 Aug 2022 15:05:40: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 15:05:40: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 02 Aug 2022 15:05:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 15:05:40: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:05:40: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:05:44: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:06:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10_peaks.xls INFO @ Tue, 02 Aug 2022 15:06:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:06:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.10_summits.bed INFO @ Tue, 02 Aug 2022 15:06:01: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (10472 records, 4 fields): 27 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:06:11: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:06:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20_peaks.xls INFO @ Tue, 02 Aug 2022 15:06:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:06:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12261368/SRX12261368.20_summits.bed INFO @ Tue, 02 Aug 2022 15:06:28: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2546 records, 4 fields): 4 millis CompletedMACS2peakCalling