Job ID = 16437904 SRX = SRX12261338 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 28653044 spots for SRR15972594/SRR15972594.sra Written 28653044 spots for SRR15972594/SRR15972594.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438095 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:14 28653044 reads; of these: 28653044 (100.00%) were unpaired; of these: 1134183 (3.96%) aligned 0 times 18744136 (65.42%) aligned exactly 1 time 8774725 (30.62%) aligned >1 times 96.04% overall alignment rate Time searching: 00:15:16 Overall time: 00:15:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13396960 / 27518861 = 0.4868 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:36:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:36:14: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:36:14: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:36:19: 1000000 INFO @ Tue, 02 Aug 2022 13:36:24: 2000000 INFO @ Tue, 02 Aug 2022 13:36:30: 3000000 INFO @ Tue, 02 Aug 2022 13:36:35: 4000000 INFO @ Tue, 02 Aug 2022 13:36:40: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:36:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:36:44: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:36:44: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:36:45: 6000000 INFO @ Tue, 02 Aug 2022 13:36:50: 1000000 INFO @ Tue, 02 Aug 2022 13:36:51: 7000000 INFO @ Tue, 02 Aug 2022 13:36:56: 8000000 INFO @ Tue, 02 Aug 2022 13:36:57: 2000000 INFO @ Tue, 02 Aug 2022 13:37:02: 9000000 INFO @ Tue, 02 Aug 2022 13:37:03: 3000000 INFO @ Tue, 02 Aug 2022 13:37:08: 10000000 INFO @ Tue, 02 Aug 2022 13:37:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:37:13: 11000000 INFO @ Tue, 02 Aug 2022 13:37:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:37:14: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:37:14: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:37:16: 5000000 INFO @ Tue, 02 Aug 2022 13:37:19: 12000000 INFO @ Tue, 02 Aug 2022 13:37:20: 1000000 INFO @ Tue, 02 Aug 2022 13:37:22: 6000000 INFO @ Tue, 02 Aug 2022 13:37:24: 13000000 INFO @ Tue, 02 Aug 2022 13:37:25: 2000000 INFO @ Tue, 02 Aug 2022 13:37:28: 7000000 INFO @ Tue, 02 Aug 2022 13:37:30: 14000000 INFO @ Tue, 02 Aug 2022 13:37:31: 3000000 INFO @ Tue, 02 Aug 2022 13:37:31: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:37:31: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:37:31: #1 total tags in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:37:31: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:37:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:37:31: #1 tags after filtering in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:37:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:37:31: #1 finished! INFO @ Tue, 02 Aug 2022 13:37:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:37:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:37:32: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 13:37:32: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:37:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:37:35: 8000000 INFO @ Tue, 02 Aug 2022 13:37:36: 4000000 INFO @ Tue, 02 Aug 2022 13:37:41: 9000000 INFO @ Tue, 02 Aug 2022 13:37:42: 5000000 INFO @ Tue, 02 Aug 2022 13:37:47: 6000000 INFO @ Tue, 02 Aug 2022 13:37:48: 10000000 INFO @ Tue, 02 Aug 2022 13:37:53: 7000000 INFO @ Tue, 02 Aug 2022 13:37:54: 11000000 INFO @ Tue, 02 Aug 2022 13:37:59: 8000000 INFO @ Tue, 02 Aug 2022 13:38:01: 12000000 INFO @ Tue, 02 Aug 2022 13:38:04: 9000000 INFO @ Tue, 02 Aug 2022 13:38:07: 13000000 INFO @ Tue, 02 Aug 2022 13:38:10: 10000000 INFO @ Tue, 02 Aug 2022 13:38:14: 14000000 INFO @ Tue, 02 Aug 2022 13:38:15: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:38:15: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:38:15: #1 total tags in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:38:15: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:38:15: #1 tags after filtering in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:38:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:38:15: #1 finished! INFO @ Tue, 02 Aug 2022 13:38:15: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:38:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:38:15: 11000000 INFO @ Tue, 02 Aug 2022 13:38:16: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 13:38:16: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:38:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:38:21: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:38:27: 13000000 INFO @ Tue, 02 Aug 2022 13:38:32: 14000000 INFO @ Tue, 02 Aug 2022 13:38:33: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:38:33: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:38:33: #1 total tags in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:38:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:38:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:38:33: #1 tags after filtering in treatment: 14121901 INFO @ Tue, 02 Aug 2022 13:38:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:38:33: #1 finished! INFO @ Tue, 02 Aug 2022 13:38:33: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:38:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:38:34: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 13:38:34: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:38:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261338/SRX12261338.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。