Job ID = 16437873 SRX = SRX12261334 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27248107 spots for SRR15972590/SRR15972590.sra Written 27248107 spots for SRR15972590/SRR15972590.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438078 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:15:01 27248107 reads; of these: 27248107 (100.00%) were unpaired; of these: 1055641 (3.87%) aligned 0 times 18057514 (66.27%) aligned exactly 1 time 8134952 (29.86%) aligned >1 times 96.13% overall alignment rate Time searching: 00:15:02 Overall time: 00:15:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13392635 / 26192466 = 0.5113 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:32:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:32:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:32:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:32:45: 1000000 INFO @ Tue, 02 Aug 2022 13:32:52: 2000000 INFO @ Tue, 02 Aug 2022 13:32:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:33:06: 4000000 INFO @ Tue, 02 Aug 2022 13:33:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:33:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:33:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:33:14: 5000000 INFO @ Tue, 02 Aug 2022 13:33:16: 1000000 INFO @ Tue, 02 Aug 2022 13:33:22: 6000000 INFO @ Tue, 02 Aug 2022 13:33:24: 2000000 INFO @ Tue, 02 Aug 2022 13:33:29: 7000000 INFO @ Tue, 02 Aug 2022 13:33:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:33:37: 8000000 INFO @ Tue, 02 Aug 2022 13:33:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:33:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:33:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:33:39: 4000000 INFO @ Tue, 02 Aug 2022 13:33:44: 9000000 INFO @ Tue, 02 Aug 2022 13:33:45: 1000000 INFO @ Tue, 02 Aug 2022 13:33:46: 5000000 INFO @ Tue, 02 Aug 2022 13:33:52: 10000000 INFO @ Tue, 02 Aug 2022 13:33:53: 2000000 INFO @ Tue, 02 Aug 2022 13:33:54: 6000000 INFO @ Tue, 02 Aug 2022 13:34:00: 11000000 INFO @ Tue, 02 Aug 2022 13:34:01: 3000000 INFO @ Tue, 02 Aug 2022 13:34:01: 7000000 INFO @ Tue, 02 Aug 2022 13:34:08: 12000000 INFO @ Tue, 02 Aug 2022 13:34:09: 4000000 INFO @ Tue, 02 Aug 2022 13:34:09: 8000000 INFO @ Tue, 02 Aug 2022 13:34:14: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:34:14: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:34:14: #1 total tags in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:34:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:34:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:34:14: #1 tags after filtering in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:34:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:34:14: #1 finished! INFO @ Tue, 02 Aug 2022 13:34:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:34:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:34:15: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 13:34:15: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:34:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:34:16: 5000000 INFO @ Tue, 02 Aug 2022 13:34:17: 9000000 INFO @ Tue, 02 Aug 2022 13:34:24: 6000000 INFO @ Tue, 02 Aug 2022 13:34:24: 10000000 INFO @ Tue, 02 Aug 2022 13:34:32: 7000000 INFO @ Tue, 02 Aug 2022 13:34:32: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:34:40: 8000000 INFO @ Tue, 02 Aug 2022 13:34:40: 12000000 INFO @ Tue, 02 Aug 2022 13:34:46: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:34:46: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:34:46: #1 total tags in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:34:46: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:34:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:34:47: #1 tags after filtering in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:34:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:34:47: #1 finished! INFO @ Tue, 02 Aug 2022 13:34:47: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:34:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:34:47: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 13:34:47: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:34:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:34:48: 9000000 INFO @ Tue, 02 Aug 2022 13:34:55: 10000000 INFO @ Tue, 02 Aug 2022 13:35:02: 11000000 INFO @ Tue, 02 Aug 2022 13:35:09: 12000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 13:35:14: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:35:14: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:35:14: #1 total tags in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:35:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:35:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:35:14: #1 tags after filtering in treatment: 12799831 INFO @ Tue, 02 Aug 2022 13:35:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:35:14: #1 finished! INFO @ Tue, 02 Aug 2022 13:35:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:35:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:35:15: #2 number of paired peaks: 76 WARNING @ Tue, 02 Aug 2022 13:35:15: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:35:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261334/SRX12261334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling