Job ID = 16437801 SRX = SRX12261333 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20701701 spots for SRR15972589/SRR15972589.sra Written 20701701 spots for SRR15972589/SRR15972589.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438082 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:52 20701701 reads; of these: 20701701 (100.00%) were unpaired; of these: 756262 (3.65%) aligned 0 times 12573402 (60.74%) aligned exactly 1 time 7372037 (35.61%) aligned >1 times 96.35% overall alignment rate Time searching: 00:15:53 Overall time: 00:15:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3928198 / 19945439 = 0.1969 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:34:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:34:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:34:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:34:31: 1000000 INFO @ Tue, 02 Aug 2022 13:34:36: 2000000 INFO @ Tue, 02 Aug 2022 13:34:41: 3000000 INFO @ Tue, 02 Aug 2022 13:34:47: 4000000 INFO @ Tue, 02 Aug 2022 13:34:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:34:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:34:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:34:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:34:57: 6000000 INFO @ Tue, 02 Aug 2022 13:35:01: 1000000 INFO @ Tue, 02 Aug 2022 13:35:03: 7000000 INFO @ Tue, 02 Aug 2022 13:35:07: 2000000 INFO @ Tue, 02 Aug 2022 13:35:08: 8000000 INFO @ Tue, 02 Aug 2022 13:35:12: 3000000 INFO @ Tue, 02 Aug 2022 13:35:14: 9000000 INFO @ Tue, 02 Aug 2022 13:35:18: 4000000 INFO @ Tue, 02 Aug 2022 13:35:19: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:35:24: 5000000 INFO @ Tue, 02 Aug 2022 13:35:25: 11000000 INFO @ Tue, 02 Aug 2022 13:35:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:35:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:35:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:35:31: 6000000 INFO @ Tue, 02 Aug 2022 13:35:31: 12000000 INFO @ Tue, 02 Aug 2022 13:35:32: 1000000 INFO @ Tue, 02 Aug 2022 13:35:38: 7000000 INFO @ Tue, 02 Aug 2022 13:35:38: 2000000 INFO @ Tue, 02 Aug 2022 13:35:39: 13000000 INFO @ Tue, 02 Aug 2022 13:35:44: 8000000 INFO @ Tue, 02 Aug 2022 13:35:45: 3000000 INFO @ Tue, 02 Aug 2022 13:35:46: 14000000 INFO @ Tue, 02 Aug 2022 13:35:51: 9000000 INFO @ Tue, 02 Aug 2022 13:35:52: 4000000 INFO @ Tue, 02 Aug 2022 13:35:53: 15000000 INFO @ Tue, 02 Aug 2022 13:35:57: 10000000 INFO @ Tue, 02 Aug 2022 13:35:59: 5000000 INFO @ Tue, 02 Aug 2022 13:35:59: 16000000 INFO @ Tue, 02 Aug 2022 13:36:00: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:36:00: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:36:00: #1 total tags in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:36:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:36:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:36:00: #1 tags after filtering in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:36:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:36:00: #1 finished! INFO @ Tue, 02 Aug 2022 13:36:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:36:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:36:01: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 13:36:01: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:36:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:36:04: 11000000 INFO @ Tue, 02 Aug 2022 13:36:06: 6000000 INFO @ Tue, 02 Aug 2022 13:36:10: 12000000 INFO @ Tue, 02 Aug 2022 13:36:12: 7000000 INFO @ Tue, 02 Aug 2022 13:36:16: 13000000 INFO @ Tue, 02 Aug 2022 13:36:18: 8000000 INFO @ Tue, 02 Aug 2022 13:36:23: 14000000 INFO @ Tue, 02 Aug 2022 13:36:25: 9000000 INFO @ Tue, 02 Aug 2022 13:36:29: 15000000 INFO @ Tue, 02 Aug 2022 13:36:32: 10000000 INFO @ Tue, 02 Aug 2022 13:36:35: 16000000 INFO @ Tue, 02 Aug 2022 13:36:36: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:36:36: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:36:36: #1 total tags in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:36:36: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:36:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:36:36: #1 tags after filtering in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:36:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:36:36: #1 finished! INFO @ Tue, 02 Aug 2022 13:36:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:36:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:36:37: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 13:36:37: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:36:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:36:38: 11000000 INFO @ Tue, 02 Aug 2022 13:36:44: 12000000 INFO @ Tue, 02 Aug 2022 13:36:51: 13000000 INFO @ Tue, 02 Aug 2022 13:36:57: 14000000 INFO @ Tue, 02 Aug 2022 13:37:04: 15000000 INFO @ Tue, 02 Aug 2022 13:37:10: 16000000 INFO @ Tue, 02 Aug 2022 13:37:11: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 13:37:11: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 13:37:11: #1 total tags in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:37:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:37:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:37:11: #1 tags after filtering in treatment: 16017241 INFO @ Tue, 02 Aug 2022 13:37:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:37:11: #1 finished! INFO @ Tue, 02 Aug 2022 13:37:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:37:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:37:12: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 13:37:12: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:37:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261333/SRX12261333.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。