Job ID = 16437789 SRX = SRX12261332 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26165505 spots for SRR15972588/SRR15972588.sra Written 26165505 spots for SRR15972588/SRR15972588.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438075 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:15:02 26165505 reads; of these: 26165505 (100.00%) were unpaired; of these: 1040299 (3.98%) aligned 0 times 16455678 (62.89%) aligned exactly 1 time 8669528 (33.13%) aligned >1 times 96.02% overall alignment rate Time searching: 00:15:03 Overall time: 00:15:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11050379 / 25125206 = 0.4398 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:31:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:31:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:31:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:31:32: 1000000 INFO @ Tue, 02 Aug 2022 13:31:38: 2000000 INFO @ Tue, 02 Aug 2022 13:31:45: 3000000 INFO @ Tue, 02 Aug 2022 13:31:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:31:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:31:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:31:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:31:59: 5000000 INFO @ Tue, 02 Aug 2022 13:32:04: 1000000 INFO @ Tue, 02 Aug 2022 13:32:07: 6000000 INFO @ Tue, 02 Aug 2022 13:32:13: 2000000 INFO @ Tue, 02 Aug 2022 13:32:15: 7000000 INFO @ Tue, 02 Aug 2022 13:32:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:32:24: 8000000 INFO @ Tue, 02 Aug 2022 13:32:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:32:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:32:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:32:31: 4000000 INFO @ Tue, 02 Aug 2022 13:32:33: 9000000 INFO @ Tue, 02 Aug 2022 13:32:34: 1000000 INFO @ Tue, 02 Aug 2022 13:32:39: 5000000 INFO @ Tue, 02 Aug 2022 13:32:41: 10000000 INFO @ Tue, 02 Aug 2022 13:32:43: 2000000 INFO @ Tue, 02 Aug 2022 13:32:48: 6000000 INFO @ Tue, 02 Aug 2022 13:32:50: 11000000 INFO @ Tue, 02 Aug 2022 13:32:52: 3000000 INFO @ Tue, 02 Aug 2022 13:32:58: 7000000 INFO @ Tue, 02 Aug 2022 13:32:58: 12000000 INFO @ Tue, 02 Aug 2022 13:33:01: 4000000 INFO @ Tue, 02 Aug 2022 13:33:07: 13000000 INFO @ Tue, 02 Aug 2022 13:33:07: 8000000 INFO @ Tue, 02 Aug 2022 13:33:10: 5000000 INFO @ Tue, 02 Aug 2022 13:33:15: 14000000 INFO @ Tue, 02 Aug 2022 13:33:16: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 13:33:16: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 13:33:16: #1 total tags in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:33:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:33:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:33:16: 9000000 INFO @ Tue, 02 Aug 2022 13:33:16: #1 tags after filtering in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:33:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:33:16: #1 finished! INFO @ Tue, 02 Aug 2022 13:33:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:33:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:33:17: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 13:33:17: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:33:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:33:20: 6000000 INFO @ Tue, 02 Aug 2022 13:33:25: 10000000 INFO @ Tue, 02 Aug 2022 13:33:29: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:33:34: 11000000 INFO @ Tue, 02 Aug 2022 13:33:38: 8000000 INFO @ Tue, 02 Aug 2022 13:33:44: 12000000 INFO @ Tue, 02 Aug 2022 13:33:47: 9000000 INFO @ Tue, 02 Aug 2022 13:33:53: 13000000 INFO @ Tue, 02 Aug 2022 13:33:57: 10000000 INFO @ Tue, 02 Aug 2022 13:34:02: 14000000 INFO @ Tue, 02 Aug 2022 13:34:03: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 13:34:03: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 13:34:03: #1 total tags in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:34:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:34:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:34:03: #1 tags after filtering in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:34:03: #1 finished! INFO @ Tue, 02 Aug 2022 13:34:03: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:34:04: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 13:34:04: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:34:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:34:06: 11000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 13:34:14: 12000000 INFO @ Tue, 02 Aug 2022 13:34:23: 13000000 INFO @ Tue, 02 Aug 2022 13:34:31: 14000000 INFO @ Tue, 02 Aug 2022 13:34:32: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 13:34:32: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 13:34:32: #1 total tags in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:34:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:34:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:34:32: #1 tags after filtering in treatment: 14074827 INFO @ Tue, 02 Aug 2022 13:34:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 13:34:32: #1 finished! INFO @ Tue, 02 Aug 2022 13:34:32: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:34:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:34:33: #2 number of paired peaks: 92 WARNING @ Tue, 02 Aug 2022 13:34:33: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:34:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX12261332/SRX12261332.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling