Job ID = 16440163 SRX = SRX12178835 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T08:24:22 prefetch.2.10.7: 1) Downloading 'SRR15888079'... 2022-08-02T08:24:22 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:26:40 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:26:40 prefetch.2.10.7: 1) 'SRR15888079' was downloaded successfully 2022-08-02T08:26:40 prefetch.2.10.7: 'SRR15888079' has 0 unresolved dependencies Read 45711202 spots for SRR15888079/SRR15888079.sra Written 45711202 spots for SRR15888079/SRR15888079.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440964 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:56 45711202 reads; of these: 45711202 (100.00%) were paired; of these: 16116933 (35.26%) aligned concordantly 0 times 25979313 (56.83%) aligned concordantly exactly 1 time 3614956 (7.91%) aligned concordantly >1 times ---- 16116933 pairs aligned concordantly 0 times; of these: 6781717 (42.08%) aligned discordantly 1 time ---- 9335216 pairs aligned 0 times concordantly or discordantly; of these: 18670432 mates make up the pairs; of these: 10529136 (56.39%) aligned 0 times 6147299 (32.93%) aligned exactly 1 time 1993997 (10.68%) aligned >1 times 88.48% overall alignment rate Time searching: 00:41:56 Overall time: 00:41:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5232240 / 36284574 = 0.1442 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:31:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:31:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:31:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:31:40: 1000000 INFO @ Tue, 02 Aug 2022 18:31:46: 2000000 INFO @ Tue, 02 Aug 2022 18:31:51: 3000000 INFO @ Tue, 02 Aug 2022 18:31:57: 4000000 INFO @ Tue, 02 Aug 2022 18:32:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:32:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:32:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:32:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:32:07: 6000000 INFO @ Tue, 02 Aug 2022 18:32:10: 1000000 INFO @ Tue, 02 Aug 2022 18:32:13: 7000000 INFO @ Tue, 02 Aug 2022 18:32:16: 2000000 INFO @ Tue, 02 Aug 2022 18:32:19: 8000000 INFO @ Tue, 02 Aug 2022 18:32:21: 3000000 INFO @ Tue, 02 Aug 2022 18:32:25: 9000000 INFO @ Tue, 02 Aug 2022 18:32:27: 4000000 INFO @ Tue, 02 Aug 2022 18:32:30: 10000000 INFO @ Tue, 02 Aug 2022 18:32:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:32:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:32:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:32:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:32:36: 11000000 INFO @ Tue, 02 Aug 2022 18:32:38: 6000000 INFO @ Tue, 02 Aug 2022 18:32:40: 1000000 INFO @ Tue, 02 Aug 2022 18:32:42: 12000000 INFO @ Tue, 02 Aug 2022 18:32:44: 7000000 INFO @ Tue, 02 Aug 2022 18:32:46: 2000000 INFO @ Tue, 02 Aug 2022 18:32:48: 13000000 INFO @ Tue, 02 Aug 2022 18:32:49: 8000000 INFO @ Tue, 02 Aug 2022 18:32:51: 3000000 INFO @ Tue, 02 Aug 2022 18:32:54: 14000000 INFO @ Tue, 02 Aug 2022 18:32:55: 9000000 INFO @ Tue, 02 Aug 2022 18:32:57: 4000000 INFO @ Tue, 02 Aug 2022 18:33:00: 15000000 INFO @ Tue, 02 Aug 2022 18:33:01: 10000000 INFO @ Tue, 02 Aug 2022 18:33:02: 5000000 INFO @ Tue, 02 Aug 2022 18:33:06: 16000000 INFO @ Tue, 02 Aug 2022 18:33:07: 11000000 INFO @ Tue, 02 Aug 2022 18:33:07: 6000000 INFO @ Tue, 02 Aug 2022 18:33:12: 17000000 INFO @ Tue, 02 Aug 2022 18:33:13: 7000000 INFO @ Tue, 02 Aug 2022 18:33:13: 12000000 INFO @ Tue, 02 Aug 2022 18:33:18: 18000000 INFO @ Tue, 02 Aug 2022 18:33:18: 8000000 INFO @ Tue, 02 Aug 2022 18:33:18: 13000000 INFO @ Tue, 02 Aug 2022 18:33:23: 9000000 INFO @ Tue, 02 Aug 2022 18:33:24: 19000000 INFO @ Tue, 02 Aug 2022 18:33:24: 14000000 INFO @ Tue, 02 Aug 2022 18:33:29: 10000000 INFO @ Tue, 02 Aug 2022 18:33:29: 20000000 INFO @ Tue, 02 Aug 2022 18:33:30: 15000000 INFO @ Tue, 02 Aug 2022 18:33:34: 11000000 INFO @ Tue, 02 Aug 2022 18:33:35: 21000000 INFO @ Tue, 02 Aug 2022 18:33:36: 16000000 INFO @ Tue, 02 Aug 2022 18:33:39: 12000000 INFO @ Tue, 02 Aug 2022 18:33:41: 17000000 INFO @ Tue, 02 Aug 2022 18:33:41: 22000000 INFO @ Tue, 02 Aug 2022 18:33:44: 13000000 INFO @ Tue, 02 Aug 2022 18:33:47: 18000000 INFO @ Tue, 02 Aug 2022 18:33:47: 23000000 INFO @ Tue, 02 Aug 2022 18:33:49: 14000000 INFO @ Tue, 02 Aug 2022 18:33:53: 19000000 INFO @ Tue, 02 Aug 2022 18:33:53: 24000000 INFO @ Tue, 02 Aug 2022 18:33:55: 15000000 INFO @ Tue, 02 Aug 2022 18:33:58: 20000000 INFO @ Tue, 02 Aug 2022 18:33:59: 25000000 INFO @ Tue, 02 Aug 2022 18:34:00: 16000000 INFO @ Tue, 02 Aug 2022 18:34:04: 21000000 INFO @ Tue, 02 Aug 2022 18:34:05: 26000000 INFO @ Tue, 02 Aug 2022 18:34:05: 17000000 INFO @ Tue, 02 Aug 2022 18:34:10: 22000000 INFO @ Tue, 02 Aug 2022 18:34:10: 18000000 INFO @ Tue, 02 Aug 2022 18:34:10: 27000000 INFO @ Tue, 02 Aug 2022 18:34:16: 19000000 INFO @ Tue, 02 Aug 2022 18:34:16: 23000000 INFO @ Tue, 02 Aug 2022 18:34:16: 28000000 INFO @ Tue, 02 Aug 2022 18:34:21: 20000000 INFO @ Tue, 02 Aug 2022 18:34:22: 24000000 INFO @ Tue, 02 Aug 2022 18:34:22: 29000000 INFO @ Tue, 02 Aug 2022 18:34:26: 21000000 INFO @ Tue, 02 Aug 2022 18:34:27: 25000000 INFO @ Tue, 02 Aug 2022 18:34:28: 30000000 INFO @ Tue, 02 Aug 2022 18:34:31: 22000000 INFO @ Tue, 02 Aug 2022 18:34:33: 26000000 INFO @ Tue, 02 Aug 2022 18:34:33: 31000000 INFO @ Tue, 02 Aug 2022 18:34:37: 23000000 INFO @ Tue, 02 Aug 2022 18:34:39: 27000000 INFO @ Tue, 02 Aug 2022 18:34:39: 32000000 INFO @ Tue, 02 Aug 2022 18:34:42: 24000000 INFO @ Tue, 02 Aug 2022 18:34:45: 28000000 INFO @ Tue, 02 Aug 2022 18:34:45: 33000000 INFO @ Tue, 02 Aug 2022 18:34:47: 25000000 INFO @ Tue, 02 Aug 2022 18:34:50: 29000000 INFO @ Tue, 02 Aug 2022 18:34:50: 34000000 INFO @ Tue, 02 Aug 2022 18:34:53: 26000000 INFO @ Tue, 02 Aug 2022 18:34:56: 30000000 INFO @ Tue, 02 Aug 2022 18:34:56: 35000000 INFO @ Tue, 02 Aug 2022 18:34:58: 27000000 INFO @ Tue, 02 Aug 2022 18:35:02: 31000000 INFO @ Tue, 02 Aug 2022 18:35:02: 36000000 INFO @ Tue, 02 Aug 2022 18:35:03: 28000000 INFO @ Tue, 02 Aug 2022 18:35:07: 32000000 INFO @ Tue, 02 Aug 2022 18:35:08: 37000000 INFO @ Tue, 02 Aug 2022 18:35:08: 29000000 INFO @ Tue, 02 Aug 2022 18:35:13: 33000000 INFO @ Tue, 02 Aug 2022 18:35:14: 38000000 INFO @ Tue, 02 Aug 2022 18:35:14: 30000000 INFO @ Tue, 02 Aug 2022 18:35:18: 34000000 INFO @ Tue, 02 Aug 2022 18:35:19: 31000000 INFO @ Tue, 02 Aug 2022 18:35:19: 39000000 INFO @ Tue, 02 Aug 2022 18:35:24: 35000000 INFO @ Tue, 02 Aug 2022 18:35:24: 32000000 INFO @ Tue, 02 Aug 2022 18:35:25: 40000000 INFO @ Tue, 02 Aug 2022 18:35:29: 36000000 INFO @ Tue, 02 Aug 2022 18:35:29: 33000000 INFO @ Tue, 02 Aug 2022 18:35:31: 41000000 INFO @ Tue, 02 Aug 2022 18:35:35: 34000000 INFO @ Tue, 02 Aug 2022 18:35:35: 37000000 INFO @ Tue, 02 Aug 2022 18:35:36: 42000000 INFO @ Tue, 02 Aug 2022 18:35:40: 35000000 INFO @ Tue, 02 Aug 2022 18:35:41: 38000000 INFO @ Tue, 02 Aug 2022 18:35:42: 43000000 INFO @ Tue, 02 Aug 2022 18:35:45: 36000000 INFO @ Tue, 02 Aug 2022 18:35:46: 39000000 INFO @ Tue, 02 Aug 2022 18:35:47: 44000000 INFO @ Tue, 02 Aug 2022 18:35:51: 37000000 INFO @ Tue, 02 Aug 2022 18:35:52: 40000000 INFO @ Tue, 02 Aug 2022 18:35:53: 45000000 INFO @ Tue, 02 Aug 2022 18:35:56: 38000000 INFO @ Tue, 02 Aug 2022 18:35:57: 41000000 INFO @ Tue, 02 Aug 2022 18:35:58: 46000000 INFO @ Tue, 02 Aug 2022 18:36:01: 39000000 INFO @ Tue, 02 Aug 2022 18:36:03: 42000000 INFO @ Tue, 02 Aug 2022 18:36:04: 47000000 INFO @ Tue, 02 Aug 2022 18:36:06: 40000000 INFO @ Tue, 02 Aug 2022 18:36:08: 43000000 INFO @ Tue, 02 Aug 2022 18:36:09: 48000000 INFO @ Tue, 02 Aug 2022 18:36:12: 41000000 INFO @ Tue, 02 Aug 2022 18:36:14: 44000000 INFO @ Tue, 02 Aug 2022 18:36:15: 49000000 INFO @ Tue, 02 Aug 2022 18:36:17: 42000000 INFO @ Tue, 02 Aug 2022 18:36:19: 45000000 INFO @ Tue, 02 Aug 2022 18:36:20: 50000000 INFO @ Tue, 02 Aug 2022 18:36:23: 43000000 INFO @ Tue, 02 Aug 2022 18:36:25: 46000000 INFO @ Tue, 02 Aug 2022 18:36:26: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:36:28: 44000000 INFO @ Tue, 02 Aug 2022 18:36:31: 47000000 INFO @ Tue, 02 Aug 2022 18:36:32: 52000000 INFO @ Tue, 02 Aug 2022 18:36:34: 45000000 INFO @ Tue, 02 Aug 2022 18:36:36: 48000000 INFO @ Tue, 02 Aug 2022 18:36:38: 53000000 INFO @ Tue, 02 Aug 2022 18:36:40: 46000000 INFO @ Tue, 02 Aug 2022 18:36:42: 49000000 INFO @ Tue, 02 Aug 2022 18:36:43: 54000000 INFO @ Tue, 02 Aug 2022 18:36:45: 47000000 INFO @ Tue, 02 Aug 2022 18:36:48: 50000000 INFO @ Tue, 02 Aug 2022 18:36:49: 55000000 INFO @ Tue, 02 Aug 2022 18:36:51: 48000000 INFO @ Tue, 02 Aug 2022 18:36:53: 51000000 INFO @ Tue, 02 Aug 2022 18:36:55: 56000000 INFO @ Tue, 02 Aug 2022 18:36:56: 49000000 INFO @ Tue, 02 Aug 2022 18:36:59: 52000000 INFO @ Tue, 02 Aug 2022 18:37:01: 57000000 INFO @ Tue, 02 Aug 2022 18:37:02: 50000000 INFO @ Tue, 02 Aug 2022 18:37:04: 53000000 INFO @ Tue, 02 Aug 2022 18:37:06: 58000000 INFO @ Tue, 02 Aug 2022 18:37:08: 51000000 INFO @ Tue, 02 Aug 2022 18:37:09: 54000000 INFO @ Tue, 02 Aug 2022 18:37:12: 59000000 INFO @ Tue, 02 Aug 2022 18:37:13: 52000000 INFO @ Tue, 02 Aug 2022 18:37:14: 55000000 INFO @ Tue, 02 Aug 2022 18:37:17: 60000000 INFO @ Tue, 02 Aug 2022 18:37:19: 53000000 INFO @ Tue, 02 Aug 2022 18:37:20: 56000000 INFO @ Tue, 02 Aug 2022 18:37:23: 61000000 INFO @ Tue, 02 Aug 2022 18:37:24: 54000000 INFO @ Tue, 02 Aug 2022 18:37:25: 57000000 INFO @ Tue, 02 Aug 2022 18:37:29: 62000000 INFO @ Tue, 02 Aug 2022 18:37:30: 58000000 INFO @ Tue, 02 Aug 2022 18:37:30: 55000000 INFO @ Tue, 02 Aug 2022 18:37:35: 63000000 INFO @ Tue, 02 Aug 2022 18:37:35: 59000000 INFO @ Tue, 02 Aug 2022 18:37:36: 56000000 INFO @ Tue, 02 Aug 2022 18:37:41: 60000000 INFO @ Tue, 02 Aug 2022 18:37:41: 64000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:37:41: 57000000 INFO @ Tue, 02 Aug 2022 18:37:46: 61000000 INFO @ Tue, 02 Aug 2022 18:37:47: 65000000 INFO @ Tue, 02 Aug 2022 18:37:47: 58000000 INFO @ Tue, 02 Aug 2022 18:37:51: 62000000 INFO @ Tue, 02 Aug 2022 18:37:52: 66000000 INFO @ Tue, 02 Aug 2022 18:37:52: 59000000 INFO @ Tue, 02 Aug 2022 18:37:56: 63000000 INFO @ Tue, 02 Aug 2022 18:37:58: 67000000 INFO @ Tue, 02 Aug 2022 18:37:58: 60000000 INFO @ Tue, 02 Aug 2022 18:38:02: 64000000 INFO @ Tue, 02 Aug 2022 18:38:04: 68000000 INFO @ Tue, 02 Aug 2022 18:38:04: 61000000 INFO @ Tue, 02 Aug 2022 18:38:07: 65000000 INFO @ Tue, 02 Aug 2022 18:38:09: 69000000 INFO @ Tue, 02 Aug 2022 18:38:10: 62000000 INFO @ Tue, 02 Aug 2022 18:38:12: 66000000 INFO @ Tue, 02 Aug 2022 18:38:15: 70000000 INFO @ Tue, 02 Aug 2022 18:38:15: 63000000 INFO @ Tue, 02 Aug 2022 18:38:17: 67000000 INFO @ Tue, 02 Aug 2022 18:38:18: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:38:18: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:38:18: #1 total tags in treatment: 24988148 INFO @ Tue, 02 Aug 2022 18:38:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:38:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:38:18: #1 tags after filtering in treatment: 15921252 INFO @ Tue, 02 Aug 2022 18:38:18: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:38:18: #1 finished! INFO @ Tue, 02 Aug 2022 18:38:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:38:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:38:19: #2 number of paired peaks: 981 WARNING @ Tue, 02 Aug 2022 18:38:19: Fewer paired peaks (981) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 981 pairs to build model! INFO @ Tue, 02 Aug 2022 18:38:19: start model_add_line... INFO @ Tue, 02 Aug 2022 18:38:19: start X-correlation... INFO @ Tue, 02 Aug 2022 18:38:19: end of X-cor INFO @ Tue, 02 Aug 2022 18:38:19: #2 finished! INFO @ Tue, 02 Aug 2022 18:38:19: #2 predicted fragment length is 68 bps INFO @ Tue, 02 Aug 2022 18:38:19: #2 alternative fragment length(s) may be 4,68 bps INFO @ Tue, 02 Aug 2022 18:38:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05_model.r WARNING @ Tue, 02 Aug 2022 18:38:19: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:38:19: #2 You may need to consider one of the other alternative d(s): 4,68 WARNING @ Tue, 02 Aug 2022 18:38:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:38:19: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:38:19: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:38:21: 64000000 INFO @ Tue, 02 Aug 2022 18:38:23: 68000000 INFO @ Tue, 02 Aug 2022 18:38:26: 65000000 INFO @ Tue, 02 Aug 2022 18:38:28: 69000000 INFO @ Tue, 02 Aug 2022 18:38:32: 66000000 INFO @ Tue, 02 Aug 2022 18:38:33: 70000000 INFO @ Tue, 02 Aug 2022 18:38:35: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:38:35: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:38:35: #1 total tags in treatment: 24988148 INFO @ Tue, 02 Aug 2022 18:38:35: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:38:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:38:36: #1 tags after filtering in treatment: 15921252 INFO @ Tue, 02 Aug 2022 18:38:36: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:38:36: #1 finished! INFO @ Tue, 02 Aug 2022 18:38:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:38:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:38:37: #2 number of paired peaks: 981 WARNING @ Tue, 02 Aug 2022 18:38:37: Fewer paired peaks (981) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 981 pairs to build model! INFO @ Tue, 02 Aug 2022 18:38:37: start model_add_line... INFO @ Tue, 02 Aug 2022 18:38:37: start X-correlation... INFO @ Tue, 02 Aug 2022 18:38:37: end of X-cor INFO @ Tue, 02 Aug 2022 18:38:37: #2 finished! INFO @ Tue, 02 Aug 2022 18:38:37: #2 predicted fragment length is 68 bps INFO @ Tue, 02 Aug 2022 18:38:37: #2 alternative fragment length(s) may be 4,68 bps INFO @ Tue, 02 Aug 2022 18:38:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10_model.r WARNING @ Tue, 02 Aug 2022 18:38:37: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:38:37: #2 You may need to consider one of the other alternative d(s): 4,68 WARNING @ Tue, 02 Aug 2022 18:38:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:38:37: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:38:37: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:38:37: 67000000 INFO @ Tue, 02 Aug 2022 18:38:43: 68000000 INFO @ Tue, 02 Aug 2022 18:38:48: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:38:48: 69000000 INFO @ Tue, 02 Aug 2022 18:38:53: 70000000 INFO @ Tue, 02 Aug 2022 18:38:56: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:38:56: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:38:56: #1 total tags in treatment: 24988148 INFO @ Tue, 02 Aug 2022 18:38:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:38:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:38:56: #1 tags after filtering in treatment: 15921252 INFO @ Tue, 02 Aug 2022 18:38:56: #1 Redundant rate of treatment: 0.36 INFO @ Tue, 02 Aug 2022 18:38:56: #1 finished! INFO @ Tue, 02 Aug 2022 18:38:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:38:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:38:57: #2 number of paired peaks: 981 WARNING @ Tue, 02 Aug 2022 18:38:57: Fewer paired peaks (981) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 981 pairs to build model! INFO @ Tue, 02 Aug 2022 18:38:57: start model_add_line... INFO @ Tue, 02 Aug 2022 18:38:58: start X-correlation... INFO @ Tue, 02 Aug 2022 18:38:58: end of X-cor INFO @ Tue, 02 Aug 2022 18:38:58: #2 finished! INFO @ Tue, 02 Aug 2022 18:38:58: #2 predicted fragment length is 68 bps INFO @ Tue, 02 Aug 2022 18:38:58: #2 alternative fragment length(s) may be 4,68 bps INFO @ Tue, 02 Aug 2022 18:38:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20_model.r WARNING @ Tue, 02 Aug 2022 18:38:58: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:38:58: #2 You may need to consider one of the other alternative d(s): 4,68 WARNING @ Tue, 02 Aug 2022 18:38:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:38:58: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:38:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:39:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05_peaks.xls INFO @ Tue, 02 Aug 2022 18:39:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:39:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.05_summits.bed INFO @ Tue, 02 Aug 2022 18:39:03: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (19990 records, 4 fields): 27 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:39:05: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:39:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10_peaks.xls INFO @ Tue, 02 Aug 2022 18:39:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:39:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.10_summits.bed INFO @ Tue, 02 Aug 2022 18:39:20: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (9975 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:39:28: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:39:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20_peaks.xls INFO @ Tue, 02 Aug 2022 18:39:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:39:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178835/SRX12178835.20_summits.bed INFO @ Tue, 02 Aug 2022 18:39:43: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1498 records, 4 fields): 15 millis CompletedMACS2peakCalling