Job ID = 16440161 SRX = SRX12178833 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T08:24:22 prefetch.2.10.7: 1) Downloading 'SRR15888081'... 2022-08-02T08:24:22 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:26:59 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:26:59 prefetch.2.10.7: 1) 'SRR15888081' was downloaded successfully 2022-08-02T08:27:00 prefetch.2.10.7: 'SRR15888081' has 0 unresolved dependencies Read 58382807 spots for SRR15888081/SRR15888081.sra Written 58382807 spots for SRR15888081/SRR15888081.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441203 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:19:47 58382807 reads; of these: 58382807 (100.00%) were paired; of these: 18386348 (31.49%) aligned concordantly 0 times 30355404 (51.99%) aligned concordantly exactly 1 time 9641055 (16.51%) aligned concordantly >1 times ---- 18386348 pairs aligned concordantly 0 times; of these: 6998530 (38.06%) aligned discordantly 1 time ---- 11387818 pairs aligned 0 times concordantly or discordantly; of these: 22775636 mates make up the pairs; of these: 13871112 (60.90%) aligned 0 times 6081931 (26.70%) aligned exactly 1 time 2822593 (12.39%) aligned >1 times 88.12% overall alignment rate Time searching: 01:19:47 Overall time: 01:19:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 9988471 / 46838767 = 0.2133 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:24:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:24:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:24:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:24:53: 1000000 INFO @ Tue, 02 Aug 2022 19:24:59: 2000000 INFO @ Tue, 02 Aug 2022 19:25:06: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:25:13: 4000000 INFO @ Tue, 02 Aug 2022 19:25:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:25:15: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:25:15: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:25:20: 5000000 INFO @ Tue, 02 Aug 2022 19:25:22: 1000000 INFO @ Tue, 02 Aug 2022 19:25:28: 6000000 INFO @ Tue, 02 Aug 2022 19:25:29: 2000000 INFO @ Tue, 02 Aug 2022 19:25:35: 7000000 INFO @ Tue, 02 Aug 2022 19:25:36: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:25:43: 8000000 INFO @ Tue, 02 Aug 2022 19:25:43: 4000000 INFO @ Tue, 02 Aug 2022 19:25:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:25:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:25:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:25:51: 9000000 INFO @ Tue, 02 Aug 2022 19:25:51: 5000000 INFO @ Tue, 02 Aug 2022 19:25:53: 1000000 INFO @ Tue, 02 Aug 2022 19:25:59: 6000000 INFO @ Tue, 02 Aug 2022 19:25:59: 10000000 INFO @ Tue, 02 Aug 2022 19:26:00: 2000000 INFO @ Tue, 02 Aug 2022 19:26:07: 7000000 INFO @ Tue, 02 Aug 2022 19:26:07: 11000000 INFO @ Tue, 02 Aug 2022 19:26:08: 3000000 INFO @ Tue, 02 Aug 2022 19:26:15: 8000000 INFO @ Tue, 02 Aug 2022 19:26:16: 12000000 INFO @ Tue, 02 Aug 2022 19:26:17: 4000000 INFO @ Tue, 02 Aug 2022 19:26:24: 9000000 INFO @ Tue, 02 Aug 2022 19:26:24: 13000000 INFO @ Tue, 02 Aug 2022 19:26:25: 5000000 INFO @ Tue, 02 Aug 2022 19:26:32: 10000000 INFO @ Tue, 02 Aug 2022 19:26:32: 14000000 INFO @ Tue, 02 Aug 2022 19:26:33: 6000000 INFO @ Tue, 02 Aug 2022 19:26:40: 11000000 INFO @ Tue, 02 Aug 2022 19:26:41: 15000000 INFO @ Tue, 02 Aug 2022 19:26:42: 7000000 INFO @ Tue, 02 Aug 2022 19:26:48: 16000000 INFO @ Tue, 02 Aug 2022 19:26:48: 12000000 INFO @ Tue, 02 Aug 2022 19:26:50: 8000000 INFO @ Tue, 02 Aug 2022 19:26:57: 17000000 INFO @ Tue, 02 Aug 2022 19:26:57: 13000000 INFO @ Tue, 02 Aug 2022 19:26:58: 9000000 INFO @ Tue, 02 Aug 2022 19:27:05: 18000000 INFO @ Tue, 02 Aug 2022 19:27:05: 14000000 INFO @ Tue, 02 Aug 2022 19:27:06: 10000000 INFO @ Tue, 02 Aug 2022 19:27:13: 19000000 INFO @ Tue, 02 Aug 2022 19:27:13: 15000000 INFO @ Tue, 02 Aug 2022 19:27:14: 11000000 INFO @ Tue, 02 Aug 2022 19:27:21: 20000000 INFO @ Tue, 02 Aug 2022 19:27:22: 16000000 INFO @ Tue, 02 Aug 2022 19:27:22: 12000000 INFO @ Tue, 02 Aug 2022 19:27:30: 21000000 INFO @ Tue, 02 Aug 2022 19:27:30: 17000000 INFO @ Tue, 02 Aug 2022 19:27:31: 13000000 INFO @ Tue, 02 Aug 2022 19:27:38: 22000000 INFO @ Tue, 02 Aug 2022 19:27:38: 18000000 INFO @ Tue, 02 Aug 2022 19:27:39: 14000000 INFO @ Tue, 02 Aug 2022 19:27:46: 23000000 INFO @ Tue, 02 Aug 2022 19:27:47: 19000000 INFO @ Tue, 02 Aug 2022 19:27:47: 15000000 INFO @ Tue, 02 Aug 2022 19:27:55: 24000000 INFO @ Tue, 02 Aug 2022 19:27:55: 20000000 INFO @ Tue, 02 Aug 2022 19:27:56: 16000000 INFO @ Tue, 02 Aug 2022 19:28:03: 25000000 INFO @ Tue, 02 Aug 2022 19:28:03: 21000000 INFO @ Tue, 02 Aug 2022 19:28:04: 17000000 INFO @ Tue, 02 Aug 2022 19:28:11: 26000000 INFO @ Tue, 02 Aug 2022 19:28:12: 22000000 INFO @ Tue, 02 Aug 2022 19:28:12: 18000000 INFO @ Tue, 02 Aug 2022 19:28:19: 27000000 INFO @ Tue, 02 Aug 2022 19:28:20: 23000000 INFO @ Tue, 02 Aug 2022 19:28:21: 19000000 INFO @ Tue, 02 Aug 2022 19:28:28: 28000000 INFO @ Tue, 02 Aug 2022 19:28:28: 24000000 INFO @ Tue, 02 Aug 2022 19:28:29: 20000000 INFO @ Tue, 02 Aug 2022 19:28:36: 29000000 INFO @ Tue, 02 Aug 2022 19:28:37: 25000000 INFO @ Tue, 02 Aug 2022 19:28:37: 21000000 INFO @ Tue, 02 Aug 2022 19:28:45: 30000000 INFO @ Tue, 02 Aug 2022 19:28:45: 26000000 INFO @ Tue, 02 Aug 2022 19:28:46: 22000000 INFO @ Tue, 02 Aug 2022 19:28:53: 31000000 INFO @ Tue, 02 Aug 2022 19:28:53: 27000000 INFO @ Tue, 02 Aug 2022 19:28:54: 23000000 INFO @ Tue, 02 Aug 2022 19:29:01: 32000000 INFO @ Tue, 02 Aug 2022 19:29:02: 28000000 INFO @ Tue, 02 Aug 2022 19:29:02: 24000000 INFO @ Tue, 02 Aug 2022 19:29:09: 33000000 INFO @ Tue, 02 Aug 2022 19:29:10: 29000000 INFO @ Tue, 02 Aug 2022 19:29:11: 25000000 INFO @ Tue, 02 Aug 2022 19:29:17: 34000000 INFO @ Tue, 02 Aug 2022 19:29:18: 30000000 INFO @ Tue, 02 Aug 2022 19:29:19: 26000000 INFO @ Tue, 02 Aug 2022 19:29:25: 35000000 INFO @ Tue, 02 Aug 2022 19:29:26: 31000000 INFO @ Tue, 02 Aug 2022 19:29:27: 27000000 INFO @ Tue, 02 Aug 2022 19:29:33: 36000000 INFO @ Tue, 02 Aug 2022 19:29:34: 32000000 INFO @ Tue, 02 Aug 2022 19:29:35: 28000000 INFO @ Tue, 02 Aug 2022 19:29:41: 37000000 INFO @ Tue, 02 Aug 2022 19:29:42: 33000000 INFO @ Tue, 02 Aug 2022 19:29:43: 29000000 INFO @ Tue, 02 Aug 2022 19:29:49: 38000000 INFO @ Tue, 02 Aug 2022 19:29:50: 34000000 INFO @ Tue, 02 Aug 2022 19:29:51: 30000000 INFO @ Tue, 02 Aug 2022 19:29:57: 39000000 INFO @ Tue, 02 Aug 2022 19:29:58: 35000000 INFO @ Tue, 02 Aug 2022 19:29:59: 31000000 INFO @ Tue, 02 Aug 2022 19:30:05: 40000000 INFO @ Tue, 02 Aug 2022 19:30:05: 36000000 INFO @ Tue, 02 Aug 2022 19:30:06: 32000000 INFO @ Tue, 02 Aug 2022 19:30:12: 41000000 INFO @ Tue, 02 Aug 2022 19:30:13: 37000000 INFO @ Tue, 02 Aug 2022 19:30:14: 33000000 INFO @ Tue, 02 Aug 2022 19:30:20: 42000000 INFO @ Tue, 02 Aug 2022 19:30:21: 38000000 INFO @ Tue, 02 Aug 2022 19:30:22: 34000000 INFO @ Tue, 02 Aug 2022 19:30:28: 43000000 INFO @ Tue, 02 Aug 2022 19:30:29: 39000000 INFO @ Tue, 02 Aug 2022 19:30:30: 35000000 INFO @ Tue, 02 Aug 2022 19:30:36: 44000000 INFO @ Tue, 02 Aug 2022 19:30:36: 40000000 INFO @ Tue, 02 Aug 2022 19:30:37: 36000000 INFO @ Tue, 02 Aug 2022 19:30:43: 45000000 INFO @ Tue, 02 Aug 2022 19:30:44: 41000000 INFO @ Tue, 02 Aug 2022 19:30:45: 37000000 INFO @ Tue, 02 Aug 2022 19:30:51: 46000000 INFO @ Tue, 02 Aug 2022 19:30:52: 42000000 INFO @ Tue, 02 Aug 2022 19:30:53: 38000000 INFO @ Tue, 02 Aug 2022 19:30:59: 47000000 INFO @ Tue, 02 Aug 2022 19:30:59: 43000000 INFO @ Tue, 02 Aug 2022 19:31:01: 39000000 INFO @ Tue, 02 Aug 2022 19:31:07: 48000000 INFO @ Tue, 02 Aug 2022 19:31:07: 44000000 INFO @ Tue, 02 Aug 2022 19:31:08: 40000000 INFO @ Tue, 02 Aug 2022 19:31:14: 49000000 INFO @ Tue, 02 Aug 2022 19:31:15: 45000000 INFO @ Tue, 02 Aug 2022 19:31:16: 41000000 INFO @ Tue, 02 Aug 2022 19:31:22: 50000000 INFO @ Tue, 02 Aug 2022 19:31:23: 46000000 INFO @ Tue, 02 Aug 2022 19:31:24: 42000000 INFO @ Tue, 02 Aug 2022 19:31:30: 51000000 INFO @ Tue, 02 Aug 2022 19:31:31: 47000000 INFO @ Tue, 02 Aug 2022 19:31:32: 43000000 INFO @ Tue, 02 Aug 2022 19:31:38: 52000000 INFO @ Tue, 02 Aug 2022 19:31:38: 48000000 INFO @ Tue, 02 Aug 2022 19:31:39: 44000000 INFO @ Tue, 02 Aug 2022 19:31:45: 53000000 INFO @ Tue, 02 Aug 2022 19:31:46: 49000000 INFO @ Tue, 02 Aug 2022 19:31:47: 45000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:31:53: 54000000 INFO @ Tue, 02 Aug 2022 19:31:54: 50000000 INFO @ Tue, 02 Aug 2022 19:31:55: 46000000 INFO @ Tue, 02 Aug 2022 19:32:01: 55000000 INFO @ Tue, 02 Aug 2022 19:32:01: 51000000 INFO @ Tue, 02 Aug 2022 19:32:02: 47000000 INFO @ Tue, 02 Aug 2022 19:32:08: 56000000 INFO @ Tue, 02 Aug 2022 19:32:09: 52000000 INFO @ Tue, 02 Aug 2022 19:32:10: 48000000 INFO @ Tue, 02 Aug 2022 19:32:16: 57000000 INFO @ Tue, 02 Aug 2022 19:32:16: 53000000 INFO @ Tue, 02 Aug 2022 19:32:17: 49000000 INFO @ Tue, 02 Aug 2022 19:32:23: 58000000 INFO @ Tue, 02 Aug 2022 19:32:24: 54000000 INFO @ Tue, 02 Aug 2022 19:32:24: 50000000 INFO @ Tue, 02 Aug 2022 19:32:31: 59000000 INFO @ Tue, 02 Aug 2022 19:32:31: 55000000 INFO @ Tue, 02 Aug 2022 19:32:32: 51000000 INFO @ Tue, 02 Aug 2022 19:32:38: 60000000 INFO @ Tue, 02 Aug 2022 19:32:39: 56000000 INFO @ Tue, 02 Aug 2022 19:32:39: 52000000 INFO @ Tue, 02 Aug 2022 19:32:46: 61000000 INFO @ Tue, 02 Aug 2022 19:32:46: 57000000 INFO @ Tue, 02 Aug 2022 19:32:47: 53000000 INFO @ Tue, 02 Aug 2022 19:32:53: 62000000 INFO @ Tue, 02 Aug 2022 19:32:54: 58000000 INFO @ Tue, 02 Aug 2022 19:32:54: 54000000 INFO @ Tue, 02 Aug 2022 19:33:01: 63000000 INFO @ Tue, 02 Aug 2022 19:33:01: 59000000 INFO @ Tue, 02 Aug 2022 19:33:01: 55000000 INFO @ Tue, 02 Aug 2022 19:33:08: 64000000 INFO @ Tue, 02 Aug 2022 19:33:08: 60000000 INFO @ Tue, 02 Aug 2022 19:33:09: 56000000 INFO @ Tue, 02 Aug 2022 19:33:16: 65000000 INFO @ Tue, 02 Aug 2022 19:33:16: 61000000 INFO @ Tue, 02 Aug 2022 19:33:16: 57000000 INFO @ Tue, 02 Aug 2022 19:33:23: 62000000 INFO @ Tue, 02 Aug 2022 19:33:24: 66000000 INFO @ Tue, 02 Aug 2022 19:33:24: 58000000 INFO @ Tue, 02 Aug 2022 19:33:30: 63000000 INFO @ Tue, 02 Aug 2022 19:33:31: 59000000 INFO @ Tue, 02 Aug 2022 19:33:31: 67000000 INFO @ Tue, 02 Aug 2022 19:33:38: 64000000 INFO @ Tue, 02 Aug 2022 19:33:38: 60000000 INFO @ Tue, 02 Aug 2022 19:33:39: 68000000 INFO @ Tue, 02 Aug 2022 19:33:45: 65000000 INFO @ Tue, 02 Aug 2022 19:33:46: 61000000 INFO @ Tue, 02 Aug 2022 19:33:47: 69000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:33:53: 66000000 INFO @ Tue, 02 Aug 2022 19:33:53: 62000000 INFO @ Tue, 02 Aug 2022 19:33:55: 70000000 INFO @ Tue, 02 Aug 2022 19:34:01: 63000000 INFO @ Tue, 02 Aug 2022 19:34:01: 67000000 INFO @ Tue, 02 Aug 2022 19:34:02: 71000000 INFO @ Tue, 02 Aug 2022 19:34:08: 64000000 INFO @ Tue, 02 Aug 2022 19:34:08: 68000000 INFO @ Tue, 02 Aug 2022 19:34:10: 72000000 INFO @ Tue, 02 Aug 2022 19:34:15: 65000000 INFO @ Tue, 02 Aug 2022 19:34:16: 69000000 INFO @ Tue, 02 Aug 2022 19:34:18: 73000000 INFO @ Tue, 02 Aug 2022 19:34:23: 66000000 INFO @ Tue, 02 Aug 2022 19:34:23: 70000000 INFO @ Tue, 02 Aug 2022 19:34:25: 74000000 INFO @ Tue, 02 Aug 2022 19:34:30: 67000000 INFO @ Tue, 02 Aug 2022 19:34:31: 71000000 INFO @ Tue, 02 Aug 2022 19:34:33: 75000000 INFO @ Tue, 02 Aug 2022 19:34:38: 68000000 INFO @ Tue, 02 Aug 2022 19:34:39: 72000000 INFO @ Tue, 02 Aug 2022 19:34:41: 76000000 INFO @ Tue, 02 Aug 2022 19:34:46: 69000000 INFO @ Tue, 02 Aug 2022 19:34:46: 73000000 INFO @ Tue, 02 Aug 2022 19:34:49: 77000000 INFO @ Tue, 02 Aug 2022 19:34:54: 70000000 INFO @ Tue, 02 Aug 2022 19:34:54: 74000000 INFO @ Tue, 02 Aug 2022 19:34:57: 78000000 INFO @ Tue, 02 Aug 2022 19:35:01: 71000000 INFO @ Tue, 02 Aug 2022 19:35:02: 75000000 INFO @ Tue, 02 Aug 2022 19:35:05: 79000000 INFO @ Tue, 02 Aug 2022 19:35:09: 72000000 INFO @ Tue, 02 Aug 2022 19:35:10: 76000000 INFO @ Tue, 02 Aug 2022 19:35:12: 80000000 INFO @ Tue, 02 Aug 2022 19:35:17: 73000000 INFO @ Tue, 02 Aug 2022 19:35:18: 77000000 INFO @ Tue, 02 Aug 2022 19:35:20: 81000000 INFO @ Tue, 02 Aug 2022 19:35:25: 74000000 INFO @ Tue, 02 Aug 2022 19:35:26: 78000000 INFO @ Tue, 02 Aug 2022 19:35:28: 82000000 INFO @ Tue, 02 Aug 2022 19:35:33: 75000000 INFO @ Tue, 02 Aug 2022 19:35:34: 79000000 INFO @ Tue, 02 Aug 2022 19:35:36: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:35:36: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:35:36: #1 total tags in treatment: 30727776 INFO @ Tue, 02 Aug 2022 19:35:36: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:35:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:35:36: #1 tags after filtering in treatment: 20364139 INFO @ Tue, 02 Aug 2022 19:35:36: #1 Redundant rate of treatment: 0.34 INFO @ Tue, 02 Aug 2022 19:35:36: #1 finished! INFO @ Tue, 02 Aug 2022 19:35:36: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:35:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:35:38: #2 number of paired peaks: 152 WARNING @ Tue, 02 Aug 2022 19:35:38: Fewer paired peaks (152) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 152 pairs to build model! INFO @ Tue, 02 Aug 2022 19:35:38: start model_add_line... INFO @ Tue, 02 Aug 2022 19:35:38: start X-correlation... INFO @ Tue, 02 Aug 2022 19:35:38: end of X-cor INFO @ Tue, 02 Aug 2022 19:35:38: #2 finished! INFO @ Tue, 02 Aug 2022 19:35:38: #2 predicted fragment length is 88 bps INFO @ Tue, 02 Aug 2022 19:35:38: #2 alternative fragment length(s) may be 3,71,88,117 bps INFO @ Tue, 02 Aug 2022 19:35:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05_model.r WARNING @ Tue, 02 Aug 2022 19:35:38: #2 Since the d (88) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:35:38: #2 You may need to consider one of the other alternative d(s): 3,71,88,117 WARNING @ Tue, 02 Aug 2022 19:35:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:35:38: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:35:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:35:40: 76000000 INFO @ Tue, 02 Aug 2022 19:35:41: 80000000 INFO @ Tue, 02 Aug 2022 19:35:48: 77000000 INFO @ Tue, 02 Aug 2022 19:35:49: 81000000 INFO @ Tue, 02 Aug 2022 19:35:56: 78000000 INFO @ Tue, 02 Aug 2022 19:35:57: 82000000 INFO @ Tue, 02 Aug 2022 19:36:04: 79000000 INFO @ Tue, 02 Aug 2022 19:36:04: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:36:04: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:36:04: #1 total tags in treatment: 30727776 INFO @ Tue, 02 Aug 2022 19:36:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:36:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:36:05: #1 tags after filtering in treatment: 20364139 INFO @ Tue, 02 Aug 2022 19:36:05: #1 Redundant rate of treatment: 0.34 INFO @ Tue, 02 Aug 2022 19:36:05: #1 finished! INFO @ Tue, 02 Aug 2022 19:36:05: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:36:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:36:06: #2 number of paired peaks: 152 WARNING @ Tue, 02 Aug 2022 19:36:06: Fewer paired peaks (152) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 152 pairs to build model! INFO @ Tue, 02 Aug 2022 19:36:06: start model_add_line... INFO @ Tue, 02 Aug 2022 19:36:07: start X-correlation... INFO @ Tue, 02 Aug 2022 19:36:07: end of X-cor INFO @ Tue, 02 Aug 2022 19:36:07: #2 finished! INFO @ Tue, 02 Aug 2022 19:36:07: #2 predicted fragment length is 88 bps INFO @ Tue, 02 Aug 2022 19:36:07: #2 alternative fragment length(s) may be 3,71,88,117 bps INFO @ Tue, 02 Aug 2022 19:36:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10_model.r WARNING @ Tue, 02 Aug 2022 19:36:07: #2 Since the d (88) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:36:07: #2 You may need to consider one of the other alternative d(s): 3,71,88,117 WARNING @ Tue, 02 Aug 2022 19:36:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:36:07: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:36:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:36:11: 80000000 INFO @ Tue, 02 Aug 2022 19:36:15: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:36:19: 81000000 INFO @ Tue, 02 Aug 2022 19:36:27: 82000000 INFO @ Tue, 02 Aug 2022 19:36:34: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 19:36:34: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 19:36:34: #1 total tags in treatment: 30727776 INFO @ Tue, 02 Aug 2022 19:36:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:36:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:36:35: #1 tags after filtering in treatment: 20364139 INFO @ Tue, 02 Aug 2022 19:36:35: #1 Redundant rate of treatment: 0.34 INFO @ Tue, 02 Aug 2022 19:36:35: #1 finished! INFO @ Tue, 02 Aug 2022 19:36:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:36:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:36:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05_peaks.xls INFO @ Tue, 02 Aug 2022 19:36:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:36:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.05_summits.bed INFO @ Tue, 02 Aug 2022 19:36:36: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (13669 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:36:36: #2 number of paired peaks: 152 WARNING @ Tue, 02 Aug 2022 19:36:36: Fewer paired peaks (152) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 152 pairs to build model! INFO @ Tue, 02 Aug 2022 19:36:36: start model_add_line... INFO @ Tue, 02 Aug 2022 19:36:36: start X-correlation... INFO @ Tue, 02 Aug 2022 19:36:36: end of X-cor INFO @ Tue, 02 Aug 2022 19:36:36: #2 finished! INFO @ Tue, 02 Aug 2022 19:36:36: #2 predicted fragment length is 88 bps INFO @ Tue, 02 Aug 2022 19:36:36: #2 alternative fragment length(s) may be 3,71,88,117 bps INFO @ Tue, 02 Aug 2022 19:36:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20_model.r WARNING @ Tue, 02 Aug 2022 19:36:36: #2 Since the d (88) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:36:36: #2 You may need to consider one of the other alternative d(s): 3,71,88,117 WARNING @ Tue, 02 Aug 2022 19:36:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:36:36: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:36:36: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:36:43: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:37:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10_peaks.xls INFO @ Tue, 02 Aug 2022 19:37:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:37:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.10_summits.bed INFO @ Tue, 02 Aug 2022 19:37:03: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7330 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:37:13: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:37:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20_peaks.xls INFO @ Tue, 02 Aug 2022 19:37:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:37:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178833/SRX12178833.20_summits.bed INFO @ Tue, 02 Aug 2022 19:37:33: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1697 records, 4 fields): 4 millis CompletedMACS2peakCalling