Job ID = 16440150 SRX = SRX12178826 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T08:21:55 prefetch.2.10.7: 1) Downloading 'SRR15888088'... 2022-08-02T08:21:55 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:26:26 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:26:26 prefetch.2.10.7: 1) 'SRR15888088' was downloaded successfully 2022-08-02T08:26:26 prefetch.2.10.7: 'SRR15888088' has 0 unresolved dependencies Read 55166418 spots for SRR15888088/SRR15888088.sra Written 55166418 spots for SRR15888088/SRR15888088.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441003 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:50:30 55166418 reads; of these: 55166418 (100.00%) were paired; of these: 17768748 (32.21%) aligned concordantly 0 times 32066884 (58.13%) aligned concordantly exactly 1 time 5330786 (9.66%) aligned concordantly >1 times ---- 17768748 pairs aligned concordantly 0 times; of these: 7338680 (41.30%) aligned discordantly 1 time ---- 10430068 pairs aligned 0 times concordantly or discordantly; of these: 20860136 mates make up the pairs; of these: 11672819 (55.96%) aligned 0 times 6782362 (32.51%) aligned exactly 1 time 2404955 (11.53%) aligned >1 times 89.42% overall alignment rate Time searching: 00:50:30 Overall time: 00:50:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8358335 / 44621040 = 0.1873 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:44:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:44:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:44:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:44:22: 1000000 INFO @ Tue, 02 Aug 2022 18:44:27: 2000000 INFO @ Tue, 02 Aug 2022 18:44:31: 3000000 INFO @ Tue, 02 Aug 2022 18:44:35: 4000000 INFO @ Tue, 02 Aug 2022 18:44:39: 5000000 INFO @ Tue, 02 Aug 2022 18:44:44: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:44:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:44:48: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:44:48: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:44:48: 7000000 INFO @ Tue, 02 Aug 2022 18:44:53: 8000000 INFO @ Tue, 02 Aug 2022 18:44:53: 1000000 INFO @ Tue, 02 Aug 2022 18:44:57: 9000000 INFO @ Tue, 02 Aug 2022 18:44:59: 2000000 INFO @ Tue, 02 Aug 2022 18:45:02: 10000000 INFO @ Tue, 02 Aug 2022 18:45:04: 3000000 INFO @ Tue, 02 Aug 2022 18:45:07: 11000000 INFO @ Tue, 02 Aug 2022 18:45:09: 4000000 INFO @ Tue, 02 Aug 2022 18:45:12: 12000000 INFO @ Tue, 02 Aug 2022 18:45:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:45:17: 13000000 INFO @ Tue, 02 Aug 2022 18:45:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:45:18: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:45:18: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:45:20: 6000000 INFO @ Tue, 02 Aug 2022 18:45:21: 14000000 INFO @ Tue, 02 Aug 2022 18:45:23: 1000000 INFO @ Tue, 02 Aug 2022 18:45:25: 7000000 INFO @ Tue, 02 Aug 2022 18:45:26: 15000000 INFO @ Tue, 02 Aug 2022 18:45:29: 2000000 INFO @ Tue, 02 Aug 2022 18:45:30: 8000000 INFO @ Tue, 02 Aug 2022 18:45:31: 16000000 INFO @ Tue, 02 Aug 2022 18:45:34: 3000000 INFO @ Tue, 02 Aug 2022 18:45:36: 17000000 INFO @ Tue, 02 Aug 2022 18:45:36: 9000000 INFO @ Tue, 02 Aug 2022 18:45:39: 4000000 INFO @ Tue, 02 Aug 2022 18:45:40: 18000000 INFO @ Tue, 02 Aug 2022 18:45:41: 10000000 INFO @ Tue, 02 Aug 2022 18:45:44: 5000000 INFO @ Tue, 02 Aug 2022 18:45:45: 19000000 INFO @ Tue, 02 Aug 2022 18:45:47: 11000000 INFO @ Tue, 02 Aug 2022 18:45:49: 6000000 INFO @ Tue, 02 Aug 2022 18:45:50: 20000000 INFO @ Tue, 02 Aug 2022 18:45:52: 12000000 INFO @ Tue, 02 Aug 2022 18:45:54: 21000000 INFO @ Tue, 02 Aug 2022 18:45:55: 7000000 INFO @ Tue, 02 Aug 2022 18:45:58: 13000000 INFO @ Tue, 02 Aug 2022 18:45:59: 22000000 INFO @ Tue, 02 Aug 2022 18:46:00: 8000000 INFO @ Tue, 02 Aug 2022 18:46:03: 14000000 INFO @ Tue, 02 Aug 2022 18:46:04: 23000000 INFO @ Tue, 02 Aug 2022 18:46:06: 9000000 INFO @ Tue, 02 Aug 2022 18:46:08: 15000000 INFO @ Tue, 02 Aug 2022 18:46:09: 24000000 INFO @ Tue, 02 Aug 2022 18:46:11: 10000000 INFO @ Tue, 02 Aug 2022 18:46:13: 25000000 INFO @ Tue, 02 Aug 2022 18:46:14: 16000000 INFO @ Tue, 02 Aug 2022 18:46:16: 11000000 INFO @ Tue, 02 Aug 2022 18:46:18: 26000000 INFO @ Tue, 02 Aug 2022 18:46:19: 17000000 INFO @ Tue, 02 Aug 2022 18:46:22: 12000000 INFO @ Tue, 02 Aug 2022 18:46:23: 27000000 INFO @ Tue, 02 Aug 2022 18:46:24: 18000000 INFO @ Tue, 02 Aug 2022 18:46:27: 13000000 INFO @ Tue, 02 Aug 2022 18:46:27: 28000000 INFO @ Tue, 02 Aug 2022 18:46:30: 19000000 INFO @ Tue, 02 Aug 2022 18:46:32: 29000000 INFO @ Tue, 02 Aug 2022 18:46:33: 14000000 INFO @ Tue, 02 Aug 2022 18:46:35: 20000000 INFO @ Tue, 02 Aug 2022 18:46:37: 30000000 INFO @ Tue, 02 Aug 2022 18:46:38: 15000000 INFO @ Tue, 02 Aug 2022 18:46:40: 21000000 INFO @ Tue, 02 Aug 2022 18:46:41: 31000000 INFO @ Tue, 02 Aug 2022 18:46:43: 16000000 INFO @ Tue, 02 Aug 2022 18:46:46: 22000000 INFO @ Tue, 02 Aug 2022 18:46:46: 32000000 INFO @ Tue, 02 Aug 2022 18:46:49: 17000000 INFO @ Tue, 02 Aug 2022 18:46:51: 33000000 INFO @ Tue, 02 Aug 2022 18:46:51: 23000000 INFO @ Tue, 02 Aug 2022 18:46:54: 18000000 INFO @ Tue, 02 Aug 2022 18:46:55: 34000000 INFO @ Tue, 02 Aug 2022 18:46:56: 24000000 INFO @ Tue, 02 Aug 2022 18:46:59: 19000000 INFO @ Tue, 02 Aug 2022 18:47:00: 35000000 INFO @ Tue, 02 Aug 2022 18:47:02: 25000000 INFO @ Tue, 02 Aug 2022 18:47:05: 20000000 INFO @ Tue, 02 Aug 2022 18:47:05: 36000000 INFO @ Tue, 02 Aug 2022 18:47:07: 26000000 INFO @ Tue, 02 Aug 2022 18:47:09: 37000000 INFO @ Tue, 02 Aug 2022 18:47:10: 21000000 INFO @ Tue, 02 Aug 2022 18:47:12: 27000000 INFO @ Tue, 02 Aug 2022 18:47:14: 38000000 INFO @ Tue, 02 Aug 2022 18:47:15: 22000000 INFO @ Tue, 02 Aug 2022 18:47:17: 28000000 INFO @ Tue, 02 Aug 2022 18:47:19: 39000000 INFO @ Tue, 02 Aug 2022 18:47:21: 23000000 INFO @ Tue, 02 Aug 2022 18:47:22: 29000000 INFO @ Tue, 02 Aug 2022 18:47:23: 40000000 INFO @ Tue, 02 Aug 2022 18:47:26: 24000000 INFO @ Tue, 02 Aug 2022 18:47:28: 41000000 INFO @ Tue, 02 Aug 2022 18:47:28: 30000000 INFO @ Tue, 02 Aug 2022 18:47:31: 25000000 INFO @ Tue, 02 Aug 2022 18:47:32: 42000000 INFO @ Tue, 02 Aug 2022 18:47:33: 31000000 INFO @ Tue, 02 Aug 2022 18:47:36: 26000000 INFO @ Tue, 02 Aug 2022 18:47:36: 43000000 INFO @ Tue, 02 Aug 2022 18:47:38: 32000000 INFO @ Tue, 02 Aug 2022 18:47:41: 44000000 INFO @ Tue, 02 Aug 2022 18:47:42: 27000000 INFO @ Tue, 02 Aug 2022 18:47:43: 33000000 INFO @ Tue, 02 Aug 2022 18:47:45: 45000000 INFO @ Tue, 02 Aug 2022 18:47:47: 28000000 INFO @ Tue, 02 Aug 2022 18:47:48: 34000000 INFO @ Tue, 02 Aug 2022 18:47:50: 46000000 INFO @ Tue, 02 Aug 2022 18:47:52: 29000000 INFO @ Tue, 02 Aug 2022 18:47:54: 35000000 INFO @ Tue, 02 Aug 2022 18:47:54: 47000000 INFO @ Tue, 02 Aug 2022 18:47:57: 30000000 INFO @ Tue, 02 Aug 2022 18:47:59: 36000000 INFO @ Tue, 02 Aug 2022 18:47:59: 48000000 INFO @ Tue, 02 Aug 2022 18:48:02: 31000000 INFO @ Tue, 02 Aug 2022 18:48:03: 49000000 INFO @ Tue, 02 Aug 2022 18:48:04: 37000000 INFO @ Tue, 02 Aug 2022 18:48:07: 32000000 INFO @ Tue, 02 Aug 2022 18:48:08: 50000000 INFO @ Tue, 02 Aug 2022 18:48:09: 38000000 INFO @ Tue, 02 Aug 2022 18:48:12: 51000000 INFO @ Tue, 02 Aug 2022 18:48:13: 33000000 INFO @ Tue, 02 Aug 2022 18:48:14: 39000000 INFO @ Tue, 02 Aug 2022 18:48:17: 52000000 INFO @ Tue, 02 Aug 2022 18:48:18: 34000000 INFO @ Tue, 02 Aug 2022 18:48:19: 40000000 INFO @ Tue, 02 Aug 2022 18:48:21: 53000000 INFO @ Tue, 02 Aug 2022 18:48:23: 35000000 INFO @ Tue, 02 Aug 2022 18:48:25: 41000000 INFO @ Tue, 02 Aug 2022 18:48:26: 54000000 INFO @ Tue, 02 Aug 2022 18:48:28: 36000000 INFO @ Tue, 02 Aug 2022 18:48:30: 42000000 INFO @ Tue, 02 Aug 2022 18:48:30: 55000000 INFO @ Tue, 02 Aug 2022 18:48:34: 37000000 INFO @ Tue, 02 Aug 2022 18:48:35: 56000000 INFO @ Tue, 02 Aug 2022 18:48:35: 43000000 INFO @ Tue, 02 Aug 2022 18:48:39: 38000000 INFO @ Tue, 02 Aug 2022 18:48:39: 57000000 INFO @ Tue, 02 Aug 2022 18:48:40: 44000000 INFO @ Tue, 02 Aug 2022 18:48:44: 58000000 INFO @ Tue, 02 Aug 2022 18:48:44: 39000000 INFO @ Tue, 02 Aug 2022 18:48:45: 45000000 INFO @ Tue, 02 Aug 2022 18:48:48: 59000000 INFO @ Tue, 02 Aug 2022 18:48:49: 40000000 INFO @ Tue, 02 Aug 2022 18:48:50: 46000000 INFO @ Tue, 02 Aug 2022 18:48:53: 60000000 INFO @ Tue, 02 Aug 2022 18:48:54: 41000000 INFO @ Tue, 02 Aug 2022 18:48:56: 47000000 INFO @ Tue, 02 Aug 2022 18:48:58: 61000000 INFO @ Tue, 02 Aug 2022 18:48:59: 42000000 INFO @ Tue, 02 Aug 2022 18:49:01: 48000000 INFO @ Tue, 02 Aug 2022 18:49:02: 62000000 INFO @ Tue, 02 Aug 2022 18:49:05: 43000000 INFO @ Tue, 02 Aug 2022 18:49:06: 49000000 INFO @ Tue, 02 Aug 2022 18:49:07: 63000000 INFO @ Tue, 02 Aug 2022 18:49:10: 44000000 INFO @ Tue, 02 Aug 2022 18:49:11: 50000000 INFO @ Tue, 02 Aug 2022 18:49:12: 64000000 INFO @ Tue, 02 Aug 2022 18:49:15: 45000000 INFO @ Tue, 02 Aug 2022 18:49:16: 65000000 INFO @ Tue, 02 Aug 2022 18:49:16: 51000000 INFO @ Tue, 02 Aug 2022 18:49:20: 46000000 INFO @ Tue, 02 Aug 2022 18:49:21: 66000000 INFO @ Tue, 02 Aug 2022 18:49:22: 52000000 INFO @ Tue, 02 Aug 2022 18:49:25: 47000000 INFO @ Tue, 02 Aug 2022 18:49:25: 67000000 INFO @ Tue, 02 Aug 2022 18:49:27: 53000000 INFO @ Tue, 02 Aug 2022 18:49:30: 68000000 INFO @ Tue, 02 Aug 2022 18:49:30: 48000000 INFO @ Tue, 02 Aug 2022 18:49:32: 54000000 INFO @ Tue, 02 Aug 2022 18:49:34: 69000000 INFO @ Tue, 02 Aug 2022 18:49:35: 49000000 INFO @ Tue, 02 Aug 2022 18:49:37: 55000000 INFO @ Tue, 02 Aug 2022 18:49:39: 70000000 INFO @ Tue, 02 Aug 2022 18:49:40: 50000000 INFO @ Tue, 02 Aug 2022 18:49:42: 56000000 INFO @ Tue, 02 Aug 2022 18:49:44: 71000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:49:45: 51000000 INFO @ Tue, 02 Aug 2022 18:49:48: 57000000 INFO @ Tue, 02 Aug 2022 18:49:48: 72000000 INFO @ Tue, 02 Aug 2022 18:49:50: 52000000 INFO @ Tue, 02 Aug 2022 18:49:53: 73000000 INFO @ Tue, 02 Aug 2022 18:49:53: 58000000 INFO @ Tue, 02 Aug 2022 18:49:55: 53000000 INFO @ Tue, 02 Aug 2022 18:49:57: 74000000 INFO @ Tue, 02 Aug 2022 18:49:58: 59000000 INFO @ Tue, 02 Aug 2022 18:50:00: 54000000 INFO @ Tue, 02 Aug 2022 18:50:02: 75000000 INFO @ Tue, 02 Aug 2022 18:50:03: 60000000 INFO @ Tue, 02 Aug 2022 18:50:05: 55000000 INFO @ Tue, 02 Aug 2022 18:50:06: 76000000 INFO @ Tue, 02 Aug 2022 18:50:09: 61000000 INFO @ Tue, 02 Aug 2022 18:50:10: 56000000 INFO @ Tue, 02 Aug 2022 18:50:11: 77000000 INFO @ Tue, 02 Aug 2022 18:50:14: 62000000 INFO @ Tue, 02 Aug 2022 18:50:15: 57000000 INFO @ Tue, 02 Aug 2022 18:50:15: 78000000 INFO @ Tue, 02 Aug 2022 18:50:19: 63000000 INFO @ Tue, 02 Aug 2022 18:50:20: 79000000 INFO @ Tue, 02 Aug 2022 18:50:20: 58000000 INFO @ Tue, 02 Aug 2022 18:50:24: 80000000 INFO @ Tue, 02 Aug 2022 18:50:24: 64000000 INFO @ Tue, 02 Aug 2022 18:50:25: 59000000 INFO @ Tue, 02 Aug 2022 18:50:28: 81000000 INFO @ Tue, 02 Aug 2022 18:50:29: 65000000 INFO @ Tue, 02 Aug 2022 18:50:30: 60000000 INFO @ Tue, 02 Aug 2022 18:50:33: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:50:33: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:50:33: #1 total tags in treatment: 29908963 INFO @ Tue, 02 Aug 2022 18:50:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:50:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:50:34: #1 tags after filtering in treatment: 18205091 INFO @ Tue, 02 Aug 2022 18:50:34: #1 Redundant rate of treatment: 0.39 INFO @ Tue, 02 Aug 2022 18:50:34: #1 finished! INFO @ Tue, 02 Aug 2022 18:50:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:50:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:50:34: 66000000 INFO @ Tue, 02 Aug 2022 18:50:35: 61000000 INFO @ Tue, 02 Aug 2022 18:50:35: #2 number of paired peaks: 833 WARNING @ Tue, 02 Aug 2022 18:50:35: Fewer paired peaks (833) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 833 pairs to build model! INFO @ Tue, 02 Aug 2022 18:50:35: start model_add_line... INFO @ Tue, 02 Aug 2022 18:50:35: start X-correlation... INFO @ Tue, 02 Aug 2022 18:50:35: end of X-cor INFO @ Tue, 02 Aug 2022 18:50:35: #2 finished! INFO @ Tue, 02 Aug 2022 18:50:35: #2 predicted fragment length is 72 bps INFO @ Tue, 02 Aug 2022 18:50:35: #2 alternative fragment length(s) may be 4,72,78 bps INFO @ Tue, 02 Aug 2022 18:50:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05_model.r WARNING @ Tue, 02 Aug 2022 18:50:35: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:50:35: #2 You may need to consider one of the other alternative d(s): 4,72,78 WARNING @ Tue, 02 Aug 2022 18:50:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:50:35: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:50:35: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:50:39: 67000000 INFO @ Tue, 02 Aug 2022 18:50:40: 62000000 INFO @ Tue, 02 Aug 2022 18:50:44: 68000000 INFO @ Tue, 02 Aug 2022 18:50:45: 63000000 INFO @ Tue, 02 Aug 2022 18:50:50: 69000000 INFO @ Tue, 02 Aug 2022 18:50:50: 64000000 INFO @ Tue, 02 Aug 2022 18:50:55: 70000000 INFO @ Tue, 02 Aug 2022 18:50:55: 65000000 INFO @ Tue, 02 Aug 2022 18:51:00: 66000000 INFO @ Tue, 02 Aug 2022 18:51:00: 71000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:51:05: 67000000 INFO @ Tue, 02 Aug 2022 18:51:05: 72000000 INFO @ Tue, 02 Aug 2022 18:51:07: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:51:10: 68000000 INFO @ Tue, 02 Aug 2022 18:51:11: 73000000 INFO @ Tue, 02 Aug 2022 18:51:16: 69000000 INFO @ Tue, 02 Aug 2022 18:51:16: 74000000 INFO @ Tue, 02 Aug 2022 18:51:21: 70000000 INFO @ Tue, 02 Aug 2022 18:51:21: 75000000 INFO @ Tue, 02 Aug 2022 18:51:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05_peaks.xls INFO @ Tue, 02 Aug 2022 18:51:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:51:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.05_summits.bed INFO @ Tue, 02 Aug 2022 18:51:25: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (20835 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:51:26: 76000000 INFO @ Tue, 02 Aug 2022 18:51:26: 71000000 INFO @ Tue, 02 Aug 2022 18:51:31: 77000000 INFO @ Tue, 02 Aug 2022 18:51:31: 72000000 INFO @ Tue, 02 Aug 2022 18:51:36: 78000000 INFO @ Tue, 02 Aug 2022 18:51:37: 73000000 INFO @ Tue, 02 Aug 2022 18:51:41: 79000000 INFO @ Tue, 02 Aug 2022 18:51:42: 74000000 INFO @ Tue, 02 Aug 2022 18:51:46: 80000000 INFO @ Tue, 02 Aug 2022 18:51:47: 75000000 INFO @ Tue, 02 Aug 2022 18:51:52: 81000000 INFO @ Tue, 02 Aug 2022 18:51:52: 76000000 INFO @ Tue, 02 Aug 2022 18:51:57: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:51:57: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:51:57: #1 total tags in treatment: 29908963 INFO @ Tue, 02 Aug 2022 18:51:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:51:57: 77000000 INFO @ Tue, 02 Aug 2022 18:51:57: #1 tags after filtering in treatment: 18205091 INFO @ Tue, 02 Aug 2022 18:51:57: #1 Redundant rate of treatment: 0.39 INFO @ Tue, 02 Aug 2022 18:51:57: #1 finished! INFO @ Tue, 02 Aug 2022 18:51:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:51:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:51:59: #2 number of paired peaks: 833 WARNING @ Tue, 02 Aug 2022 18:51:59: Fewer paired peaks (833) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 833 pairs to build model! INFO @ Tue, 02 Aug 2022 18:51:59: start model_add_line... INFO @ Tue, 02 Aug 2022 18:51:59: start X-correlation... INFO @ Tue, 02 Aug 2022 18:51:59: end of X-cor INFO @ Tue, 02 Aug 2022 18:51:59: #2 finished! INFO @ Tue, 02 Aug 2022 18:51:59: #2 predicted fragment length is 72 bps INFO @ Tue, 02 Aug 2022 18:51:59: #2 alternative fragment length(s) may be 4,72,78 bps INFO @ Tue, 02 Aug 2022 18:51:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10_model.r WARNING @ Tue, 02 Aug 2022 18:51:59: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:51:59: #2 You may need to consider one of the other alternative d(s): 4,72,78 WARNING @ Tue, 02 Aug 2022 18:51:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:51:59: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:51:59: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:52:02: 78000000 INFO @ Tue, 02 Aug 2022 18:52:07: 79000000 INFO @ Tue, 02 Aug 2022 18:52:12: 80000000 INFO @ Tue, 02 Aug 2022 18:52:17: 81000000 INFO @ Tue, 02 Aug 2022 18:52:22: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:52:22: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:52:22: #1 total tags in treatment: 29908963 INFO @ Tue, 02 Aug 2022 18:52:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:52:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:52:22: #1 tags after filtering in treatment: 18205091 INFO @ Tue, 02 Aug 2022 18:52:22: #1 Redundant rate of treatment: 0.39 INFO @ Tue, 02 Aug 2022 18:52:22: #1 finished! INFO @ Tue, 02 Aug 2022 18:52:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:52:24: #2 number of paired peaks: 833 WARNING @ Tue, 02 Aug 2022 18:52:24: Fewer paired peaks (833) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 833 pairs to build model! INFO @ Tue, 02 Aug 2022 18:52:24: start model_add_line... INFO @ Tue, 02 Aug 2022 18:52:24: start X-correlation... INFO @ Tue, 02 Aug 2022 18:52:24: end of X-cor INFO @ Tue, 02 Aug 2022 18:52:24: #2 finished! INFO @ Tue, 02 Aug 2022 18:52:24: #2 predicted fragment length is 72 bps INFO @ Tue, 02 Aug 2022 18:52:24: #2 alternative fragment length(s) may be 4,72,78 bps INFO @ Tue, 02 Aug 2022 18:52:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20_model.r WARNING @ Tue, 02 Aug 2022 18:52:24: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:52:24: #2 You may need to consider one of the other alternative d(s): 4,72,78 WARNING @ Tue, 02 Aug 2022 18:52:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:52:24: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:52:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:52:31: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:52:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10_peaks.xls INFO @ Tue, 02 Aug 2022 18:52:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:52:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.10_summits.bed INFO @ Tue, 02 Aug 2022 18:52:49: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (11377 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:52:57: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:53:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20_peaks.xls INFO @ Tue, 02 Aug 2022 18:53:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:53:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178826/SRX12178826.20_summits.bed INFO @ Tue, 02 Aug 2022 18:53:14: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (2471 records, 4 fields): 4 millis CompletedMACS2peakCalling