Job ID = 16440111 SRX = SRX12178814 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T08:13:04 prefetch.2.10.7: 1) Downloading 'SRR15888100'... 2022-08-02T08:13:04 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T08:15:59 prefetch.2.10.7: HTTPS download succeed 2022-08-02T08:15:59 prefetch.2.10.7: 1) 'SRR15888100' was downloaded successfully 2022-08-02T08:15:59 prefetch.2.10.7: 'SRR15888100' has 0 unresolved dependencies Read 55009088 spots for SRR15888100/SRR15888100.sra Written 55009088 spots for SRR15888100/SRR15888100.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440954 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:49:09 55009088 reads; of these: 55009088 (100.00%) were paired; of these: 13844530 (25.17%) aligned concordantly 0 times 34760059 (63.19%) aligned concordantly exactly 1 time 6404499 (11.64%) aligned concordantly >1 times ---- 13844530 pairs aligned concordantly 0 times; of these: 5120618 (36.99%) aligned discordantly 1 time ---- 8723912 pairs aligned 0 times concordantly or discordantly; of these: 17447824 mates make up the pairs; of these: 11963812 (68.57%) aligned 0 times 3938226 (22.57%) aligned exactly 1 time 1545786 (8.86%) aligned >1 times 89.13% overall alignment rate Time searching: 00:49:09 Overall time: 00:49:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 16430600 / 46086126 = 0.3565 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:29:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:29:14: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:29:14: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:29:19: 1000000 INFO @ Tue, 02 Aug 2022 18:29:23: 2000000 INFO @ Tue, 02 Aug 2022 18:29:27: 3000000 INFO @ Tue, 02 Aug 2022 18:29:31: 4000000 INFO @ Tue, 02 Aug 2022 18:29:36: 5000000 INFO @ Tue, 02 Aug 2022 18:29:40: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:29:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:29:44: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:29:44: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:29:44: 7000000 INFO @ Tue, 02 Aug 2022 18:29:48: 1000000 INFO @ Tue, 02 Aug 2022 18:29:49: 8000000 INFO @ Tue, 02 Aug 2022 18:29:53: 2000000 INFO @ Tue, 02 Aug 2022 18:29:53: 9000000 INFO @ Tue, 02 Aug 2022 18:29:57: 10000000 INFO @ Tue, 02 Aug 2022 18:29:58: 3000000 INFO @ Tue, 02 Aug 2022 18:30:02: 11000000 INFO @ Tue, 02 Aug 2022 18:30:02: 4000000 INFO @ Tue, 02 Aug 2022 18:30:06: 12000000 INFO @ Tue, 02 Aug 2022 18:30:07: 5000000 INFO @ Tue, 02 Aug 2022 18:30:10: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:30:13: 6000000 INFO @ Tue, 02 Aug 2022 18:30:15: 14000000 INFO @ Tue, 02 Aug 2022 18:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:30:15: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:30:15: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:30:18: 7000000 INFO @ Tue, 02 Aug 2022 18:30:20: 15000000 INFO @ Tue, 02 Aug 2022 18:30:20: 1000000 INFO @ Tue, 02 Aug 2022 18:30:23: 8000000 INFO @ Tue, 02 Aug 2022 18:30:25: 2000000 INFO @ Tue, 02 Aug 2022 18:30:25: 16000000 INFO @ Tue, 02 Aug 2022 18:30:27: 9000000 INFO @ Tue, 02 Aug 2022 18:30:29: 17000000 INFO @ Tue, 02 Aug 2022 18:30:29: 3000000 INFO @ Tue, 02 Aug 2022 18:30:32: 10000000 INFO @ Tue, 02 Aug 2022 18:30:34: 18000000 INFO @ Tue, 02 Aug 2022 18:30:34: 4000000 INFO @ Tue, 02 Aug 2022 18:30:37: 11000000 INFO @ Tue, 02 Aug 2022 18:30:38: 19000000 INFO @ Tue, 02 Aug 2022 18:30:39: 5000000 INFO @ Tue, 02 Aug 2022 18:30:42: 12000000 INFO @ Tue, 02 Aug 2022 18:30:43: 20000000 INFO @ Tue, 02 Aug 2022 18:30:43: 6000000 INFO @ Tue, 02 Aug 2022 18:30:47: 13000000 INFO @ Tue, 02 Aug 2022 18:30:47: 21000000 INFO @ Tue, 02 Aug 2022 18:30:48: 7000000 INFO @ Tue, 02 Aug 2022 18:30:52: 14000000 INFO @ Tue, 02 Aug 2022 18:30:52: 22000000 INFO @ Tue, 02 Aug 2022 18:30:52: 8000000 INFO @ Tue, 02 Aug 2022 18:30:56: 15000000 INFO @ Tue, 02 Aug 2022 18:30:56: 23000000 INFO @ Tue, 02 Aug 2022 18:30:57: 9000000 INFO @ Tue, 02 Aug 2022 18:31:01: 24000000 INFO @ Tue, 02 Aug 2022 18:31:01: 16000000 INFO @ Tue, 02 Aug 2022 18:31:01: 10000000 INFO @ Tue, 02 Aug 2022 18:31:05: 25000000 INFO @ Tue, 02 Aug 2022 18:31:06: 11000000 INFO @ Tue, 02 Aug 2022 18:31:06: 17000000 INFO @ Tue, 02 Aug 2022 18:31:10: 26000000 INFO @ Tue, 02 Aug 2022 18:31:10: 18000000 INFO @ Tue, 02 Aug 2022 18:31:10: 12000000 INFO @ Tue, 02 Aug 2022 18:31:14: 27000000 INFO @ Tue, 02 Aug 2022 18:31:15: 19000000 INFO @ Tue, 02 Aug 2022 18:31:15: 13000000 INFO @ Tue, 02 Aug 2022 18:31:19: 28000000 INFO @ Tue, 02 Aug 2022 18:31:19: 20000000 INFO @ Tue, 02 Aug 2022 18:31:20: 14000000 INFO @ Tue, 02 Aug 2022 18:31:23: 29000000 INFO @ Tue, 02 Aug 2022 18:31:24: 21000000 INFO @ Tue, 02 Aug 2022 18:31:24: 15000000 INFO @ Tue, 02 Aug 2022 18:31:28: 30000000 INFO @ Tue, 02 Aug 2022 18:31:29: 22000000 INFO @ Tue, 02 Aug 2022 18:31:29: 16000000 INFO @ Tue, 02 Aug 2022 18:31:32: 31000000 INFO @ Tue, 02 Aug 2022 18:31:33: 23000000 INFO @ Tue, 02 Aug 2022 18:31:34: 17000000 INFO @ Tue, 02 Aug 2022 18:31:37: 32000000 INFO @ Tue, 02 Aug 2022 18:31:38: 24000000 INFO @ Tue, 02 Aug 2022 18:31:38: 18000000 INFO @ Tue, 02 Aug 2022 18:31:41: 33000000 INFO @ Tue, 02 Aug 2022 18:31:42: 25000000 INFO @ Tue, 02 Aug 2022 18:31:43: 19000000 INFO @ Tue, 02 Aug 2022 18:31:46: 34000000 INFO @ Tue, 02 Aug 2022 18:31:47: 26000000 INFO @ Tue, 02 Aug 2022 18:31:47: 20000000 INFO @ Tue, 02 Aug 2022 18:31:50: 35000000 INFO @ Tue, 02 Aug 2022 18:31:52: 27000000 INFO @ Tue, 02 Aug 2022 18:31:52: 21000000 INFO @ Tue, 02 Aug 2022 18:31:55: 36000000 INFO @ Tue, 02 Aug 2022 18:31:56: 28000000 INFO @ Tue, 02 Aug 2022 18:31:57: 22000000 INFO @ Tue, 02 Aug 2022 18:31:59: 37000000 INFO @ Tue, 02 Aug 2022 18:32:01: 29000000 INFO @ Tue, 02 Aug 2022 18:32:01: 23000000 INFO @ Tue, 02 Aug 2022 18:32:04: 38000000 INFO @ Tue, 02 Aug 2022 18:32:05: 30000000 INFO @ Tue, 02 Aug 2022 18:32:06: 24000000 INFO @ Tue, 02 Aug 2022 18:32:08: 39000000 INFO @ Tue, 02 Aug 2022 18:32:10: 31000000 INFO @ Tue, 02 Aug 2022 18:32:10: 25000000 INFO @ Tue, 02 Aug 2022 18:32:12: 40000000 INFO @ Tue, 02 Aug 2022 18:32:15: 32000000 INFO @ Tue, 02 Aug 2022 18:32:15: 26000000 INFO @ Tue, 02 Aug 2022 18:32:17: 41000000 INFO @ Tue, 02 Aug 2022 18:32:19: 33000000 INFO @ Tue, 02 Aug 2022 18:32:20: 27000000 INFO @ Tue, 02 Aug 2022 18:32:21: 42000000 INFO @ Tue, 02 Aug 2022 18:32:24: 34000000 INFO @ Tue, 02 Aug 2022 18:32:24: 28000000 INFO @ Tue, 02 Aug 2022 18:32:26: 43000000 INFO @ Tue, 02 Aug 2022 18:32:28: 35000000 INFO @ Tue, 02 Aug 2022 18:32:29: 29000000 INFO @ Tue, 02 Aug 2022 18:32:30: 44000000 INFO @ Tue, 02 Aug 2022 18:32:33: 36000000 INFO @ Tue, 02 Aug 2022 18:32:33: 30000000 INFO @ Tue, 02 Aug 2022 18:32:35: 45000000 INFO @ Tue, 02 Aug 2022 18:32:37: 37000000 INFO @ Tue, 02 Aug 2022 18:32:38: 31000000 INFO @ Tue, 02 Aug 2022 18:32:39: 46000000 INFO @ Tue, 02 Aug 2022 18:32:42: 38000000 INFO @ Tue, 02 Aug 2022 18:32:43: 32000000 INFO @ Tue, 02 Aug 2022 18:32:44: 47000000 INFO @ Tue, 02 Aug 2022 18:32:47: 39000000 INFO @ Tue, 02 Aug 2022 18:32:47: 33000000 INFO @ Tue, 02 Aug 2022 18:32:48: 48000000 INFO @ Tue, 02 Aug 2022 18:32:51: 40000000 INFO @ Tue, 02 Aug 2022 18:32:52: 34000000 INFO @ Tue, 02 Aug 2022 18:32:52: 49000000 INFO @ Tue, 02 Aug 2022 18:32:56: 41000000 INFO @ Tue, 02 Aug 2022 18:32:56: 50000000 INFO @ Tue, 02 Aug 2022 18:32:56: 35000000 INFO @ Tue, 02 Aug 2022 18:33:00: 42000000 INFO @ Tue, 02 Aug 2022 18:33:01: 51000000 INFO @ Tue, 02 Aug 2022 18:33:01: 36000000 INFO @ Tue, 02 Aug 2022 18:33:05: 43000000 INFO @ Tue, 02 Aug 2022 18:33:05: 52000000 INFO @ Tue, 02 Aug 2022 18:33:05: 37000000 INFO @ Tue, 02 Aug 2022 18:33:09: 44000000 INFO @ Tue, 02 Aug 2022 18:33:09: 53000000 INFO @ Tue, 02 Aug 2022 18:33:10: 38000000 INFO @ Tue, 02 Aug 2022 18:33:14: 45000000 INFO @ Tue, 02 Aug 2022 18:33:14: 54000000 INFO @ Tue, 02 Aug 2022 18:33:14: 39000000 INFO @ Tue, 02 Aug 2022 18:33:18: 55000000 INFO @ Tue, 02 Aug 2022 18:33:19: 46000000 INFO @ Tue, 02 Aug 2022 18:33:19: 40000000 INFO @ Tue, 02 Aug 2022 18:33:23: 56000000 INFO @ Tue, 02 Aug 2022 18:33:23: 47000000 INFO @ Tue, 02 Aug 2022 18:33:24: 41000000 INFO @ Tue, 02 Aug 2022 18:33:27: 57000000 INFO @ Tue, 02 Aug 2022 18:33:28: 48000000 INFO @ Tue, 02 Aug 2022 18:33:28: 42000000 INFO @ Tue, 02 Aug 2022 18:33:31: 58000000 INFO @ Tue, 02 Aug 2022 18:33:32: 49000000 INFO @ Tue, 02 Aug 2022 18:33:33: 43000000 INFO @ Tue, 02 Aug 2022 18:33:36: 59000000 INFO @ Tue, 02 Aug 2022 18:33:37: 50000000 INFO @ Tue, 02 Aug 2022 18:33:37: 44000000 INFO @ Tue, 02 Aug 2022 18:33:40: 60000000 INFO @ Tue, 02 Aug 2022 18:33:41: 51000000 INFO @ Tue, 02 Aug 2022 18:33:42: 45000000 INFO @ Tue, 02 Aug 2022 18:33:44: 61000000 INFO @ Tue, 02 Aug 2022 18:33:46: 52000000 INFO @ Tue, 02 Aug 2022 18:33:47: 46000000 INFO @ Tue, 02 Aug 2022 18:33:49: 62000000 INFO @ Tue, 02 Aug 2022 18:33:51: 53000000 INFO @ Tue, 02 Aug 2022 18:33:51: 47000000 INFO @ Tue, 02 Aug 2022 18:33:53: 63000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:33:56: 54000000 INFO @ Tue, 02 Aug 2022 18:33:56: 48000000 INFO @ Tue, 02 Aug 2022 18:33:57: 64000000 INFO @ Tue, 02 Aug 2022 18:34:00: 49000000 INFO @ Tue, 02 Aug 2022 18:34:00: 55000000 INFO @ Tue, 02 Aug 2022 18:34:01: 65000000 INFO @ Tue, 02 Aug 2022 18:34:02: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:34:02: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:34:02: #1 total tags in treatment: 26002886 INFO @ Tue, 02 Aug 2022 18:34:02: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:34:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:34:03: #1 tags after filtering in treatment: 17396826 INFO @ Tue, 02 Aug 2022 18:34:03: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 18:34:03: #1 finished! INFO @ Tue, 02 Aug 2022 18:34:03: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:34:04: #2 number of paired peaks: 540 WARNING @ Tue, 02 Aug 2022 18:34:04: Fewer paired peaks (540) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 540 pairs to build model! INFO @ Tue, 02 Aug 2022 18:34:04: start model_add_line... INFO @ Tue, 02 Aug 2022 18:34:04: start X-correlation... INFO @ Tue, 02 Aug 2022 18:34:04: end of X-cor INFO @ Tue, 02 Aug 2022 18:34:04: #2 finished! INFO @ Tue, 02 Aug 2022 18:34:04: #2 predicted fragment length is 44 bps INFO @ Tue, 02 Aug 2022 18:34:04: #2 alternative fragment length(s) may be 4,44 bps INFO @ Tue, 02 Aug 2022 18:34:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05_model.r WARNING @ Tue, 02 Aug 2022 18:34:04: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:34:04: #2 You may need to consider one of the other alternative d(s): 4,44 WARNING @ Tue, 02 Aug 2022 18:34:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:34:04: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:34:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:34:05: 50000000 INFO @ Tue, 02 Aug 2022 18:34:05: 56000000 INFO @ Tue, 02 Aug 2022 18:34:09: 51000000 INFO @ Tue, 02 Aug 2022 18:34:09: 57000000 INFO @ Tue, 02 Aug 2022 18:34:14: 52000000 INFO @ Tue, 02 Aug 2022 18:34:14: 58000000 INFO @ Tue, 02 Aug 2022 18:34:18: 53000000 INFO @ Tue, 02 Aug 2022 18:34:19: 59000000 INFO @ Tue, 02 Aug 2022 18:34:23: 54000000 INFO @ Tue, 02 Aug 2022 18:34:23: 60000000 INFO @ Tue, 02 Aug 2022 18:34:27: 55000000 INFO @ Tue, 02 Aug 2022 18:34:28: 61000000 INFO @ Tue, 02 Aug 2022 18:34:32: 56000000 INFO @ Tue, 02 Aug 2022 18:34:32: 62000000 INFO @ Tue, 02 Aug 2022 18:34:36: 57000000 INFO @ Tue, 02 Aug 2022 18:34:37: 63000000 INFO @ Tue, 02 Aug 2022 18:34:37: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:34:41: 58000000 INFO @ Tue, 02 Aug 2022 18:34:41: 64000000 INFO @ Tue, 02 Aug 2022 18:34:45: 59000000 INFO @ Tue, 02 Aug 2022 18:34:46: 65000000 INFO @ Tue, 02 Aug 2022 18:34:47: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:34:47: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:34:47: #1 total tags in treatment: 26002886 INFO @ Tue, 02 Aug 2022 18:34:47: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:34:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:34:48: #1 tags after filtering in treatment: 17396826 INFO @ Tue, 02 Aug 2022 18:34:48: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 18:34:48: #1 finished! INFO @ Tue, 02 Aug 2022 18:34:48: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:34:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:34:49: #2 number of paired peaks: 540 WARNING @ Tue, 02 Aug 2022 18:34:49: Fewer paired peaks (540) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 540 pairs to build model! INFO @ Tue, 02 Aug 2022 18:34:49: start model_add_line... INFO @ Tue, 02 Aug 2022 18:34:49: start X-correlation... INFO @ Tue, 02 Aug 2022 18:34:49: end of X-cor INFO @ Tue, 02 Aug 2022 18:34:49: #2 finished! INFO @ Tue, 02 Aug 2022 18:34:49: #2 predicted fragment length is 44 bps INFO @ Tue, 02 Aug 2022 18:34:49: #2 alternative fragment length(s) may be 4,44 bps INFO @ Tue, 02 Aug 2022 18:34:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10_model.r WARNING @ Tue, 02 Aug 2022 18:34:49: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:34:49: #2 You may need to consider one of the other alternative d(s): 4,44 WARNING @ Tue, 02 Aug 2022 18:34:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:34:49: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:34:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:34:50: 60000000 INFO @ Tue, 02 Aug 2022 18:34:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05_peaks.xls INFO @ Tue, 02 Aug 2022 18:34:54: 61000000 INFO @ Tue, 02 Aug 2022 18:34:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:34:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.05_summits.bed INFO @ Tue, 02 Aug 2022 18:34:55: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (18517 records, 4 fields): 28 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:34:59: 62000000 INFO @ Tue, 02 Aug 2022 18:35:03: 63000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:35:08: 64000000 INFO @ Tue, 02 Aug 2022 18:35:12: 65000000 INFO @ Tue, 02 Aug 2022 18:35:13: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 18:35:13: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 18:35:13: #1 total tags in treatment: 26002886 INFO @ Tue, 02 Aug 2022 18:35:13: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:35:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:35:14: #1 tags after filtering in treatment: 17396826 INFO @ Tue, 02 Aug 2022 18:35:14: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 18:35:14: #1 finished! INFO @ Tue, 02 Aug 2022 18:35:14: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:35:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:35:15: #2 number of paired peaks: 540 WARNING @ Tue, 02 Aug 2022 18:35:15: Fewer paired peaks (540) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 540 pairs to build model! INFO @ Tue, 02 Aug 2022 18:35:15: start model_add_line... INFO @ Tue, 02 Aug 2022 18:35:15: start X-correlation... INFO @ Tue, 02 Aug 2022 18:35:15: end of X-cor INFO @ Tue, 02 Aug 2022 18:35:15: #2 finished! INFO @ Tue, 02 Aug 2022 18:35:15: #2 predicted fragment length is 44 bps INFO @ Tue, 02 Aug 2022 18:35:15: #2 alternative fragment length(s) may be 4,44 bps INFO @ Tue, 02 Aug 2022 18:35:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20_model.r WARNING @ Tue, 02 Aug 2022 18:35:15: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:35:15: #2 You may need to consider one of the other alternative d(s): 4,44 WARNING @ Tue, 02 Aug 2022 18:35:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:35:15: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:35:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:35:20: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:35:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10_peaks.xls INFO @ Tue, 02 Aug 2022 18:35:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:35:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.10_summits.bed INFO @ Tue, 02 Aug 2022 18:35:37: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (8057 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:35:46: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:36:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20_peaks.xls INFO @ Tue, 02 Aug 2022 18:36:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:36:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX12178814/SRX12178814.20_summits.bed INFO @ Tue, 02 Aug 2022 18:36:02: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (522 records, 4 fields): 25 millis CompletedMACS2peakCalling