Job ID = 14170758 SRX = SRX11704570 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2021-12-10T23:24:48 prefetch.2.10.7: 1) Downloading 'SRR15402354'... 2021-12-10T23:24:48 prefetch.2.10.7: Downloading via HTTPS... 2021-12-10T23:26:51 prefetch.2.10.7: HTTPS download succeed 2021-12-10T23:26:51 prefetch.2.10.7: 1) 'SRR15402354' was downloaded successfully 2021-12-10T23:26:51 prefetch.2.10.7: 'SRR15402354' has 0 unresolved dependencies Read 62085963 spots for SRR15402354/SRR15402354.sra Written 62085963 spots for SRR15402354/SRR15402354.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171550 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:07:45 62085963 reads; of these: 62085963 (100.00%) were paired; of these: 46477406 (74.86%) aligned concordantly 0 times 14113448 (22.73%) aligned concordantly exactly 1 time 1495109 (2.41%) aligned concordantly >1 times ---- 46477406 pairs aligned concordantly 0 times; of these: 3302065 (7.10%) aligned discordantly 1 time ---- 43175341 pairs aligned 0 times concordantly or discordantly; of these: 86350682 mates make up the pairs; of these: 69485853 (80.47%) aligned 0 times 1932082 (2.24%) aligned exactly 1 time 14932747 (17.29%) aligned >1 times 44.04% overall alignment rate Time searching: 01:07:45 Overall time: 01:07:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7906390 / 18547375 = 0.4263 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:09: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:09: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:14: 1000000 INFO @ Sat, 11 Dec 2021 09:48:18: 2000000 INFO @ Sat, 11 Dec 2021 09:48:24: 3000000 INFO @ Sat, 11 Dec 2021 09:48:28: 4000000 INFO @ Sat, 11 Dec 2021 09:48:32: 5000000 INFO @ Sat, 11 Dec 2021 09:48:37: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:48:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:48:39: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:48:39: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:48:41: 7000000 INFO @ Sat, 11 Dec 2021 09:48:45: 1000000 INFO @ Sat, 11 Dec 2021 09:48:47: 8000000 INFO @ Sat, 11 Dec 2021 09:48:51: 2000000 INFO @ Sat, 11 Dec 2021 09:48:51: 9000000 INFO @ Sat, 11 Dec 2021 09:48:56: 10000000 INFO @ Sat, 11 Dec 2021 09:48:56: 3000000 INFO @ Sat, 11 Dec 2021 09:49:01: 11000000 INFO @ Sat, 11 Dec 2021 09:49:02: 4000000 INFO @ Sat, 11 Dec 2021 09:49:06: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:49:09: 5000000 INFO @ Sat, 11 Dec 2021 09:49:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:49:09: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:49:09: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:49:11: 13000000 INFO @ Sat, 11 Dec 2021 09:49:14: 1000000 INFO @ Sat, 11 Dec 2021 09:49:15: 6000000 INFO @ Sat, 11 Dec 2021 09:49:16: 14000000 INFO @ Sat, 11 Dec 2021 09:49:19: 2000000 INFO @ Sat, 11 Dec 2021 09:49:21: 7000000 INFO @ Sat, 11 Dec 2021 09:49:21: 15000000 INFO @ Sat, 11 Dec 2021 09:49:24: 3000000 INFO @ Sat, 11 Dec 2021 09:49:26: 16000000 INFO @ Sat, 11 Dec 2021 09:49:26: 8000000 INFO @ Sat, 11 Dec 2021 09:49:29: 4000000 INFO @ Sat, 11 Dec 2021 09:49:30: 17000000 INFO @ Sat, 11 Dec 2021 09:49:32: 9000000 INFO @ Sat, 11 Dec 2021 09:49:34: 5000000 INFO @ Sat, 11 Dec 2021 09:49:36: 18000000 INFO @ Sat, 11 Dec 2021 09:49:38: 10000000 INFO @ Sat, 11 Dec 2021 09:49:39: 6000000 INFO @ Sat, 11 Dec 2021 09:49:40: 19000000 INFO @ Sat, 11 Dec 2021 09:49:44: 11000000 INFO @ Sat, 11 Dec 2021 09:49:45: 20000000 INFO @ Sat, 11 Dec 2021 09:49:46: 7000000 INFO @ Sat, 11 Dec 2021 09:49:50: 12000000 INFO @ Sat, 11 Dec 2021 09:49:50: 21000000 INFO @ Sat, 11 Dec 2021 09:49:52: 8000000 INFO @ Sat, 11 Dec 2021 09:49:54: 22000000 INFO @ Sat, 11 Dec 2021 09:49:56: 13000000 INFO @ Sat, 11 Dec 2021 09:49:58: 9000000 INFO @ Sat, 11 Dec 2021 09:49:59: 23000000 INFO @ Sat, 11 Dec 2021 09:50:02: 14000000 INFO @ Sat, 11 Dec 2021 09:50:04: 10000000 INFO @ Sat, 11 Dec 2021 09:50:04: 24000000 INFO @ Sat, 11 Dec 2021 09:50:08: 15000000 INFO @ Sat, 11 Dec 2021 09:50:08: 25000000 INFO @ Sat, 11 Dec 2021 09:50:10: 11000000 INFO @ Sat, 11 Dec 2021 09:50:13: 26000000 INFO @ Sat, 11 Dec 2021 09:50:15: 16000000 INFO @ Sat, 11 Dec 2021 09:50:16: 12000000 INFO @ Sat, 11 Dec 2021 09:50:17: 27000000 INFO @ Sat, 11 Dec 2021 09:50:21: 17000000 INFO @ Sat, 11 Dec 2021 09:50:21: 28000000 INFO @ Sat, 11 Dec 2021 09:50:22: 13000000 INFO @ Sat, 11 Dec 2021 09:50:25: 29000000 INFO @ Sat, 11 Dec 2021 09:50:26: 18000000 INFO @ Sat, 11 Dec 2021 09:50:27: 14000000 INFO @ Sat, 11 Dec 2021 09:50:29: 30000000 INFO @ Sat, 11 Dec 2021 09:50:32: 19000000 INFO @ Sat, 11 Dec 2021 09:50:33: 15000000 INFO @ Sat, 11 Dec 2021 09:50:34: 31000000 INFO @ Sat, 11 Dec 2021 09:50:36: 20000000 INFO @ Sat, 11 Dec 2021 09:50:39: 32000000 INFO @ Sat, 11 Dec 2021 09:50:40: 16000000 INFO @ Sat, 11 Dec 2021 09:50:41: 21000000 INFO @ Sat, 11 Dec 2021 09:50:43: 33000000 INFO @ Sat, 11 Dec 2021 09:50:45: 17000000 INFO @ Sat, 11 Dec 2021 09:50:45: 22000000 INFO @ Sat, 11 Dec 2021 09:50:47: 34000000 INFO @ Sat, 11 Dec 2021 09:50:50: 18000000 INFO @ Sat, 11 Dec 2021 09:50:51: 23000000 INFO @ Sat, 11 Dec 2021 09:50:51: 35000000 INFO @ Sat, 11 Dec 2021 09:50:55: 19000000 INFO @ Sat, 11 Dec 2021 09:50:55: 36000000 INFO @ Sat, 11 Dec 2021 09:50:56: 24000000 INFO @ Sat, 11 Dec 2021 09:51:00: 20000000 INFO @ Sat, 11 Dec 2021 09:51:00: 37000000 INFO @ Sat, 11 Dec 2021 09:51:01: 25000000 INFO @ Sat, 11 Dec 2021 09:51:04: 38000000 INFO @ Sat, 11 Dec 2021 09:51:04: 21000000 INFO @ Sat, 11 Dec 2021 09:51:06: 26000000 INFO @ Sat, 11 Dec 2021 09:51:08: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:51:08: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:51:08: #1 total tags in treatment: 9561155 INFO @ Sat, 11 Dec 2021 09:51:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:51:08: #1 tags after filtering in treatment: 5561461 INFO @ Sat, 11 Dec 2021 09:51:08: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 11 Dec 2021 09:51:08: #1 finished! INFO @ Sat, 11 Dec 2021 09:51:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:51:09: 22000000 INFO @ Sat, 11 Dec 2021 09:51:09: #2 number of paired peaks: 6866 INFO @ Sat, 11 Dec 2021 09:51:09: start model_add_line... INFO @ Sat, 11 Dec 2021 09:51:09: start X-correlation... INFO @ Sat, 11 Dec 2021 09:51:09: end of X-cor INFO @ Sat, 11 Dec 2021 09:51:09: #2 finished! INFO @ Sat, 11 Dec 2021 09:51:09: #2 predicted fragment length is 79 bps INFO @ Sat, 11 Dec 2021 09:51:09: #2 alternative fragment length(s) may be 79 bps INFO @ Sat, 11 Dec 2021 09:51:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05_model.r WARNING @ Sat, 11 Dec 2021 09:51:09: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:51:09: #2 You may need to consider one of the other alternative d(s): 79 WARNING @ Sat, 11 Dec 2021 09:51:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:51:09: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:51:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:51:10: 27000000 INFO @ Sat, 11 Dec 2021 09:51:14: 23000000 INFO @ Sat, 11 Dec 2021 09:51:15: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:51:19: 29000000 INFO @ Sat, 11 Dec 2021 09:51:20: 24000000 INFO @ Sat, 11 Dec 2021 09:51:20: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:51:24: 30000000 INFO @ Sat, 11 Dec 2021 09:51:25: 25000000 INFO @ Sat, 11 Dec 2021 09:51:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:51:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:51:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.05_summits.bed INFO @ Sat, 11 Dec 2021 09:51:26: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (16222 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:51:28: 31000000 INFO @ Sat, 11 Dec 2021 09:51:29: 26000000 INFO @ Sat, 11 Dec 2021 09:51:33: 32000000 INFO @ Sat, 11 Dec 2021 09:51:34: 27000000 INFO @ Sat, 11 Dec 2021 09:51:37: 33000000 INFO @ Sat, 11 Dec 2021 09:51:39: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:51:42: 34000000 INFO @ Sat, 11 Dec 2021 09:51:43: 29000000 INFO @ Sat, 11 Dec 2021 09:51:47: 35000000 INFO @ Sat, 11 Dec 2021 09:51:48: 30000000 INFO @ Sat, 11 Dec 2021 09:51:52: 36000000 INFO @ Sat, 11 Dec 2021 09:51:53: 31000000 INFO @ Sat, 11 Dec 2021 09:51:57: 37000000 INFO @ Sat, 11 Dec 2021 09:51:57: 32000000 INFO @ Sat, 11 Dec 2021 09:52:02: 33000000 INFO @ Sat, 11 Dec 2021 09:52:02: 38000000 INFO @ Sat, 11 Dec 2021 09:52:06: 34000000 INFO @ Sat, 11 Dec 2021 09:52:07: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:52:07: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:52:07: #1 total tags in treatment: 9561155 INFO @ Sat, 11 Dec 2021 09:52:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:52:07: #1 tags after filtering in treatment: 5561461 INFO @ Sat, 11 Dec 2021 09:52:07: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 11 Dec 2021 09:52:07: #1 finished! INFO @ Sat, 11 Dec 2021 09:52:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:52:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:52:08: #2 number of paired peaks: 6866 INFO @ Sat, 11 Dec 2021 09:52:08: start model_add_line... INFO @ Sat, 11 Dec 2021 09:52:08: start X-correlation... INFO @ Sat, 11 Dec 2021 09:52:08: end of X-cor INFO @ Sat, 11 Dec 2021 09:52:08: #2 finished! INFO @ Sat, 11 Dec 2021 09:52:08: #2 predicted fragment length is 79 bps INFO @ Sat, 11 Dec 2021 09:52:08: #2 alternative fragment length(s) may be 79 bps INFO @ Sat, 11 Dec 2021 09:52:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10_model.r WARNING @ Sat, 11 Dec 2021 09:52:08: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:52:08: #2 You may need to consider one of the other alternative d(s): 79 WARNING @ Sat, 11 Dec 2021 09:52:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:52:08: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:52:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:52:11: 35000000 INFO @ Sat, 11 Dec 2021 09:52:16: 36000000 INFO @ Sat, 11 Dec 2021 09:52:19: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:52:21: 37000000 INFO @ Sat, 11 Dec 2021 09:52:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:52:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:52:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.10_summits.bed INFO @ Sat, 11 Dec 2021 09:52:25: Done! INFO @ Sat, 11 Dec 2021 09:52:26: 38000000 pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (11660 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:52:31: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 09:52:31: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 09:52:31: #1 total tags in treatment: 9561155 INFO @ Sat, 11 Dec 2021 09:52:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:52:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:52:31: #1 tags after filtering in treatment: 5561461 INFO @ Sat, 11 Dec 2021 09:52:31: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 11 Dec 2021 09:52:31: #1 finished! INFO @ Sat, 11 Dec 2021 09:52:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:52:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:52:31: #2 number of paired peaks: 6866 INFO @ Sat, 11 Dec 2021 09:52:31: start model_add_line... INFO @ Sat, 11 Dec 2021 09:52:32: start X-correlation... INFO @ Sat, 11 Dec 2021 09:52:32: end of X-cor INFO @ Sat, 11 Dec 2021 09:52:32: #2 finished! INFO @ Sat, 11 Dec 2021 09:52:32: #2 predicted fragment length is 79 bps INFO @ Sat, 11 Dec 2021 09:52:32: #2 alternative fragment length(s) may be 79 bps INFO @ Sat, 11 Dec 2021 09:52:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20_model.r WARNING @ Sat, 11 Dec 2021 09:52:32: #2 Since the d (79) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 09:52:32: #2 You may need to consider one of the other alternative d(s): 79 WARNING @ Sat, 11 Dec 2021 09:52:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 09:52:32: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:52:32: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:52:43: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:52:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:52:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:52:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11704570/SRX11704570.20_summits.bed INFO @ Sat, 11 Dec 2021 09:52:49: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (6492 records, 4 fields): 41 millis CompletedMACS2peakCalling