Job ID = 16439622 SRX = SRX11548849 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7913008 spots for SRR15243020/SRR15243020.sra Written 7913008 spots for SRR15243020/SRR15243020.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439720 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:10 7913008 reads; of these: 7913008 (100.00%) were paired; of these: 644079 (8.14%) aligned concordantly 0 times 5747591 (72.63%) aligned concordantly exactly 1 time 1521338 (19.23%) aligned concordantly >1 times ---- 644079 pairs aligned concordantly 0 times; of these: 64961 (10.09%) aligned discordantly 1 time ---- 579118 pairs aligned 0 times concordantly or discordantly; of these: 1158236 mates make up the pairs; of these: 844884 (72.95%) aligned 0 times 167835 (14.49%) aligned exactly 1 time 145517 (12.56%) aligned >1 times 94.66% overall alignment rate Time searching: 00:15:10 Overall time: 00:15:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 181222 / 7326118 = 0.0247 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:03:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:03:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:03:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:04:02: 1000000 INFO @ Tue, 02 Aug 2022 16:04:08: 2000000 INFO @ Tue, 02 Aug 2022 16:04:14: 3000000 INFO @ Tue, 02 Aug 2022 16:04:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:04:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:04:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:04:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:04:27: 5000000 INFO @ Tue, 02 Aug 2022 16:04:32: 1000000 INFO @ Tue, 02 Aug 2022 16:04:33: 6000000 INFO @ Tue, 02 Aug 2022 16:04:38: 2000000 INFO @ Tue, 02 Aug 2022 16:04:40: 7000000 INFO @ Tue, 02 Aug 2022 16:04:45: 3000000 INFO @ Tue, 02 Aug 2022 16:04:46: 8000000 INFO @ Tue, 02 Aug 2022 16:04:51: 4000000 INFO @ Tue, 02 Aug 2022 16:04:53: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:04:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:04:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:04:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:04:58: 5000000 INFO @ Tue, 02 Aug 2022 16:05:00: 10000000 INFO @ Tue, 02 Aug 2022 16:05:03: 1000000 INFO @ Tue, 02 Aug 2022 16:05:05: 6000000 INFO @ Tue, 02 Aug 2022 16:05:07: 11000000 INFO @ Tue, 02 Aug 2022 16:05:09: 2000000 INFO @ Tue, 02 Aug 2022 16:05:12: 7000000 INFO @ Tue, 02 Aug 2022 16:05:13: 12000000 INFO @ Tue, 02 Aug 2022 16:05:15: 3000000 INFO @ Tue, 02 Aug 2022 16:05:18: 8000000 INFO @ Tue, 02 Aug 2022 16:05:20: 13000000 INFO @ Tue, 02 Aug 2022 16:05:22: 4000000 INFO @ Tue, 02 Aug 2022 16:05:25: 9000000 INFO @ Tue, 02 Aug 2022 16:05:27: 14000000 INFO @ Tue, 02 Aug 2022 16:05:28: 5000000 INFO @ Tue, 02 Aug 2022 16:05:31: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:05:31: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:05:31: #1 total tags in treatment: 7088059 INFO @ Tue, 02 Aug 2022 16:05:31: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:05:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:05:31: #1 tags after filtering in treatment: 6872843 INFO @ Tue, 02 Aug 2022 16:05:31: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:05:31: #1 finished! INFO @ Tue, 02 Aug 2022 16:05:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:05:31: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:05:31: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:05:31: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 16:05:31: 10000000 cut: /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:05:35: 6000000 INFO @ Tue, 02 Aug 2022 16:05:38: 11000000 INFO @ Tue, 02 Aug 2022 16:05:41: 7000000 INFO @ Tue, 02 Aug 2022 16:05:45: 12000000 INFO @ Tue, 02 Aug 2022 16:05:47: 8000000 INFO @ Tue, 02 Aug 2022 16:05:51: 13000000 INFO @ Tue, 02 Aug 2022 16:05:54: 9000000 INFO @ Tue, 02 Aug 2022 16:05:58: 14000000 INFO @ Tue, 02 Aug 2022 16:06:00: 10000000 INFO @ Tue, 02 Aug 2022 16:06:02: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:06:02: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:06:02: #1 total tags in treatment: 7088059 INFO @ Tue, 02 Aug 2022 16:06:02: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:06:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:06:02: #1 tags after filtering in treatment: 6872843 INFO @ Tue, 02 Aug 2022 16:06:02: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:06:02: #1 finished! INFO @ Tue, 02 Aug 2022 16:06:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:06:02: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:06:02: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:06:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:06:06: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:06:12: 12000000 INFO @ Tue, 02 Aug 2022 16:06:18: 13000000 INFO @ Tue, 02 Aug 2022 16:06:23: 14000000 INFO @ Tue, 02 Aug 2022 16:06:27: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:06:27: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:06:27: #1 total tags in treatment: 7088059 INFO @ Tue, 02 Aug 2022 16:06:27: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:06:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:06:27: #1 tags after filtering in treatment: 6872843 INFO @ Tue, 02 Aug 2022 16:06:27: #1 Redundant rate of treatment: 0.03 INFO @ Tue, 02 Aug 2022 16:06:27: #1 finished! INFO @ Tue, 02 Aug 2022 16:06:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:06:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:06:28: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:06:28: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11548849/SRX11548849.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。