Job ID = 16439699 SRX = SRX11499368 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18954466 spots for SRR15192726/SRR15192726.sra Written 18954466 spots for SRR15192726/SRR15192726.sra Read 13747212 spots for SRR15192727/SRR15192727.sra Written 13747212 spots for SRR15192727/SRR15192727.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441202 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:51:26 32701678 reads; of these: 32701678 (100.00%) were paired; of these: 4127735 (12.62%) aligned concordantly 0 times 21838080 (66.78%) aligned concordantly exactly 1 time 6735863 (20.60%) aligned concordantly >1 times ---- 4127735 pairs aligned concordantly 0 times; of these: 1829443 (44.32%) aligned discordantly 1 time ---- 2298292 pairs aligned 0 times concordantly or discordantly; of these: 4596584 mates make up the pairs; of these: 2280560 (49.61%) aligned 0 times 994722 (21.64%) aligned exactly 1 time 1321302 (28.75%) aligned >1 times 96.51% overall alignment rate Time searching: 02:51:26 Overall time: 02:51:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3523755 / 26569219 = 0.1326 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:41:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:41:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:41:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:41:23: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:41:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:41:34: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:41:34: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:41:41: 2000000 INFO @ Tue, 02 Aug 2022 19:41:55: 1000000 INFO @ Tue, 02 Aug 2022 19:42:00: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 19:42:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 19:42:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 19:42:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 19:42:14: 2000000 INFO @ Tue, 02 Aug 2022 19:42:21: 4000000 INFO @ Tue, 02 Aug 2022 19:42:26: 1000000 INFO @ Tue, 02 Aug 2022 19:42:34: 3000000 INFO @ Tue, 02 Aug 2022 19:42:40: 5000000 INFO @ Tue, 02 Aug 2022 19:42:47: 2000000 INFO @ Tue, 02 Aug 2022 19:42:54: 4000000 INFO @ Tue, 02 Aug 2022 19:43:00: 6000000 INFO @ Tue, 02 Aug 2022 19:43:07: 3000000 INFO @ Tue, 02 Aug 2022 19:43:13: 5000000 INFO @ Tue, 02 Aug 2022 19:43:21: 7000000 INFO @ Tue, 02 Aug 2022 19:43:28: 4000000 INFO @ Tue, 02 Aug 2022 19:43:33: 6000000 INFO @ Tue, 02 Aug 2022 19:43:41: 8000000 INFO @ Tue, 02 Aug 2022 19:43:49: 5000000 INFO @ Tue, 02 Aug 2022 19:43:54: 7000000 INFO @ Tue, 02 Aug 2022 19:44:00: 9000000 INFO @ Tue, 02 Aug 2022 19:44:09: 6000000 INFO @ Tue, 02 Aug 2022 19:44:13: 8000000 INFO @ Tue, 02 Aug 2022 19:44:18: 10000000 INFO @ Tue, 02 Aug 2022 19:44:30: 7000000 INFO @ Tue, 02 Aug 2022 19:44:32: 9000000 INFO @ Tue, 02 Aug 2022 19:44:38: 11000000 INFO @ Tue, 02 Aug 2022 19:44:51: 8000000 INFO @ Tue, 02 Aug 2022 19:44:53: 10000000 INFO @ Tue, 02 Aug 2022 19:44:58: 12000000 INFO @ Tue, 02 Aug 2022 19:45:11: 9000000 INFO @ Tue, 02 Aug 2022 19:45:14: 11000000 INFO @ Tue, 02 Aug 2022 19:45:19: 13000000 INFO @ Tue, 02 Aug 2022 19:45:31: 10000000 INFO @ Tue, 02 Aug 2022 19:45:35: 12000000 INFO @ Tue, 02 Aug 2022 19:45:40: 14000000 INFO @ Tue, 02 Aug 2022 19:45:52: 11000000 INFO @ Tue, 02 Aug 2022 19:45:57: 13000000 INFO @ Tue, 02 Aug 2022 19:46:00: 15000000 INFO @ Tue, 02 Aug 2022 19:46:11: 12000000 INFO @ Tue, 02 Aug 2022 19:46:17: 16000000 INFO @ Tue, 02 Aug 2022 19:46:18: 14000000 INFO @ Tue, 02 Aug 2022 19:46:31: 13000000 INFO @ Tue, 02 Aug 2022 19:46:35: 17000000 INFO @ Tue, 02 Aug 2022 19:46:39: 15000000 INFO @ Tue, 02 Aug 2022 19:46:52: 14000000 INFO @ Tue, 02 Aug 2022 19:46:53: 18000000 INFO @ Tue, 02 Aug 2022 19:46:59: 16000000 INFO @ Tue, 02 Aug 2022 19:47:12: 19000000 INFO @ Tue, 02 Aug 2022 19:47:13: 15000000 INFO @ Tue, 02 Aug 2022 19:47:19: 17000000 INFO @ Tue, 02 Aug 2022 19:47:30: 20000000 INFO @ Tue, 02 Aug 2022 19:47:32: 16000000 INFO @ Tue, 02 Aug 2022 19:47:38: 18000000 INFO @ Tue, 02 Aug 2022 19:47:49: 21000000 INFO @ Tue, 02 Aug 2022 19:47:51: 17000000 INFO @ Tue, 02 Aug 2022 19:47:57: 19000000 INFO @ Tue, 02 Aug 2022 19:48:07: 22000000 INFO @ Tue, 02 Aug 2022 19:48:11: 18000000 INFO @ Tue, 02 Aug 2022 19:48:16: 20000000 INFO @ Tue, 02 Aug 2022 19:48:27: 23000000 INFO @ Tue, 02 Aug 2022 19:48:30: 19000000 INFO @ Tue, 02 Aug 2022 19:48:36: 21000000 INFO @ Tue, 02 Aug 2022 19:48:46: 24000000 INFO @ Tue, 02 Aug 2022 19:48:49: 20000000 INFO @ Tue, 02 Aug 2022 19:48:55: 22000000 INFO @ Tue, 02 Aug 2022 19:49:04: 25000000 INFO @ Tue, 02 Aug 2022 19:49:09: 21000000 INFO @ Tue, 02 Aug 2022 19:49:15: 23000000 INFO @ Tue, 02 Aug 2022 19:49:22: 26000000 INFO @ Tue, 02 Aug 2022 19:49:29: 22000000 INFO @ Tue, 02 Aug 2022 19:49:36: 24000000 INFO @ Tue, 02 Aug 2022 19:49:41: 27000000 INFO @ Tue, 02 Aug 2022 19:49:49: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:49:58: 25000000 INFO @ Tue, 02 Aug 2022 19:50:03: 28000000 INFO @ Tue, 02 Aug 2022 19:50:09: 24000000 INFO @ Tue, 02 Aug 2022 19:50:19: 26000000 INFO @ Tue, 02 Aug 2022 19:50:24: 29000000 INFO @ Tue, 02 Aug 2022 19:50:28: 25000000 INFO @ Tue, 02 Aug 2022 19:50:40: 27000000 INFO @ Tue, 02 Aug 2022 19:50:44: 30000000 INFO @ Tue, 02 Aug 2022 19:50:48: 26000000 INFO @ Tue, 02 Aug 2022 19:51:01: 28000000 INFO @ Tue, 02 Aug 2022 19:51:03: 31000000 INFO @ Tue, 02 Aug 2022 19:51:07: 27000000 INFO @ Tue, 02 Aug 2022 19:51:21: 29000000 INFO @ Tue, 02 Aug 2022 19:51:24: 32000000 INFO @ Tue, 02 Aug 2022 19:51:26: 28000000 INFO @ Tue, 02 Aug 2022 19:51:42: 30000000 INFO @ Tue, 02 Aug 2022 19:51:45: 33000000 INFO @ Tue, 02 Aug 2022 19:51:45: 29000000 INFO @ Tue, 02 Aug 2022 19:52:03: 31000000 INFO @ Tue, 02 Aug 2022 19:52:04: 30000000 INFO @ Tue, 02 Aug 2022 19:52:06: 34000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:52:23: 31000000 INFO @ Tue, 02 Aug 2022 19:52:24: 32000000 INFO @ Tue, 02 Aug 2022 19:52:27: 35000000 INFO @ Tue, 02 Aug 2022 19:52:42: 32000000 INFO @ Tue, 02 Aug 2022 19:52:44: 33000000 INFO @ Tue, 02 Aug 2022 19:52:48: 36000000 INFO @ Tue, 02 Aug 2022 19:53:02: 33000000 INFO @ Tue, 02 Aug 2022 19:53:05: 34000000 INFO @ Tue, 02 Aug 2022 19:53:07: 37000000 INFO @ Tue, 02 Aug 2022 19:53:21: 34000000 INFO @ Tue, 02 Aug 2022 19:53:25: 35000000 INFO @ Tue, 02 Aug 2022 19:53:27: 38000000 INFO @ Tue, 02 Aug 2022 19:53:40: 35000000 INFO @ Tue, 02 Aug 2022 19:53:45: 36000000 INFO @ Tue, 02 Aug 2022 19:53:48: 39000000 INFO @ Tue, 02 Aug 2022 19:54:00: 36000000 INFO @ Tue, 02 Aug 2022 19:54:05: 37000000 INFO @ Tue, 02 Aug 2022 19:54:08: 40000000 INFO @ Tue, 02 Aug 2022 19:54:19: 37000000 INFO @ Tue, 02 Aug 2022 19:54:24: 38000000 INFO @ Tue, 02 Aug 2022 19:54:28: 41000000 INFO @ Tue, 02 Aug 2022 19:54:38: 38000000 INFO @ Tue, 02 Aug 2022 19:54:43: 39000000 INFO @ Tue, 02 Aug 2022 19:54:49: 42000000 INFO @ Tue, 02 Aug 2022 19:54:57: 39000000 INFO @ Tue, 02 Aug 2022 19:55:03: 40000000 INFO @ Tue, 02 Aug 2022 19:55:10: 43000000 INFO @ Tue, 02 Aug 2022 19:55:17: 40000000 INFO @ Tue, 02 Aug 2022 19:55:25: 41000000 INFO @ Tue, 02 Aug 2022 19:55:32: 44000000 INFO @ Tue, 02 Aug 2022 19:55:37: 41000000 INFO @ Tue, 02 Aug 2022 19:55:48: 42000000 INFO @ Tue, 02 Aug 2022 19:55:55: 45000000 INFO @ Tue, 02 Aug 2022 19:55:59: 42000000 INFO @ Tue, 02 Aug 2022 19:56:10: 43000000 INFO @ Tue, 02 Aug 2022 19:56:18: 46000000 INFO @ Tue, 02 Aug 2022 19:56:21: 43000000 INFO @ Tue, 02 Aug 2022 19:56:33: 44000000 INFO @ Tue, 02 Aug 2022 19:56:39: 47000000 INFO @ Tue, 02 Aug 2022 19:56:42: 44000000 INFO @ Tue, 02 Aug 2022 19:56:53: 45000000 INFO @ Tue, 02 Aug 2022 19:57:01: 48000000 INFO @ Tue, 02 Aug 2022 19:57:03: 45000000 INFO @ Tue, 02 Aug 2022 19:57:15: 46000000 INFO @ Tue, 02 Aug 2022 19:57:23: 46000000 INFO @ Tue, 02 Aug 2022 19:57:23: 49000000 INFO @ Tue, 02 Aug 2022 19:57:36: 47000000 INFO @ Tue, 02 Aug 2022 19:57:44: 47000000 INFO @ Tue, 02 Aug 2022 19:57:45: 50000000 INFO @ Tue, 02 Aug 2022 19:57:58: 48000000 INFO @ Tue, 02 Aug 2022 19:58:06: 48000000 INFO @ Tue, 02 Aug 2022 19:58:06: 51000000 INFO @ Tue, 02 Aug 2022 19:58:20: 49000000 INFO @ Tue, 02 Aug 2022 19:58:26: 49000000 INFO @ Tue, 02 Aug 2022 19:58:27: 52000000 INFO @ Tue, 02 Aug 2022 19:58:41: 50000000 INFO @ Tue, 02 Aug 2022 19:58:47: 50000000 INFO @ Tue, 02 Aug 2022 19:58:49: 53000000 INFO @ Tue, 02 Aug 2022 19:59:04: 51000000 INFO @ Tue, 02 Aug 2022 19:59:08: 51000000 INFO @ Tue, 02 Aug 2022 19:59:11: 54000000 INFO @ Tue, 02 Aug 2022 19:59:25: 52000000 INFO @ Tue, 02 Aug 2022 19:59:29: 52000000 INFO @ Tue, 02 Aug 2022 19:59:34: 55000000 INFO @ Tue, 02 Aug 2022 19:59:48: 53000000 INFO @ Tue, 02 Aug 2022 19:59:51: 53000000 INFO @ Tue, 02 Aug 2022 19:59:57: 56000000 INFO @ Tue, 02 Aug 2022 19:59:59: #1 tag size is determined as 132 bps INFO @ Tue, 02 Aug 2022 19:59:59: #1 tag size = 132 INFO @ Tue, 02 Aug 2022 19:59:59: #1 total tags in treatment: 25182044 INFO @ Tue, 02 Aug 2022 19:59:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:59:59: #1 tags after filtering in treatment: 22802581 INFO @ Tue, 02 Aug 2022 19:59:59: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 19:59:59: #1 finished! INFO @ Tue, 02 Aug 2022 19:59:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:00:02: #2 number of paired peaks: 199 WARNING @ Tue, 02 Aug 2022 20:00:02: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 02 Aug 2022 20:00:02: start model_add_line... INFO @ Tue, 02 Aug 2022 20:00:02: start X-correlation... INFO @ Tue, 02 Aug 2022 20:00:02: end of X-cor INFO @ Tue, 02 Aug 2022 20:00:02: #2 finished! INFO @ Tue, 02 Aug 2022 20:00:02: #2 predicted fragment length is 235 bps INFO @ Tue, 02 Aug 2022 20:00:02: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 02 Aug 2022 20:00:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05_model.r WARNING @ Tue, 02 Aug 2022 20:00:02: #2 Since the d (235) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 20:00:02: #2 You may need to consider one of the other alternative d(s): 235 WARNING @ Tue, 02 Aug 2022 20:00:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 20:00:02: #3 Call peaks... INFO @ Tue, 02 Aug 2022 20:00:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 20:00:09: 54000000 INFO @ Tue, 02 Aug 2022 20:00:11: 54000000 INFO @ Tue, 02 Aug 2022 20:00:29: 55000000 INFO @ Tue, 02 Aug 2022 20:00:31: 55000000 INFO @ Tue, 02 Aug 2022 20:00:49: 56000000 INFO @ Tue, 02 Aug 2022 20:00:51: 56000000 INFO @ Tue, 02 Aug 2022 20:00:51: #1 tag size is determined as 132 bps INFO @ Tue, 02 Aug 2022 20:00:51: #1 tag size = 132 INFO @ Tue, 02 Aug 2022 20:00:51: #1 total tags in treatment: 25182044 INFO @ Tue, 02 Aug 2022 20:00:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 20:00:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 20:00:52: #1 tags after filtering in treatment: 22802581 INFO @ Tue, 02 Aug 2022 20:00:52: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 20:00:52: #1 finished! INFO @ Tue, 02 Aug 2022 20:00:52: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 20:00:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:00:53: #1 tag size is determined as 132 bps INFO @ Tue, 02 Aug 2022 20:00:53: #1 tag size = 132 INFO @ Tue, 02 Aug 2022 20:00:53: #1 total tags in treatment: 25182044 INFO @ Tue, 02 Aug 2022 20:00:53: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 20:00:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 20:00:53: #1 tags after filtering in treatment: 22802581 INFO @ Tue, 02 Aug 2022 20:00:53: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 20:00:53: #1 finished! INFO @ Tue, 02 Aug 2022 20:00:53: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 20:00:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 20:00:54: #2 number of paired peaks: 199 WARNING @ Tue, 02 Aug 2022 20:00:54: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 02 Aug 2022 20:00:54: start model_add_line... INFO @ Tue, 02 Aug 2022 20:00:54: start X-correlation... INFO @ Tue, 02 Aug 2022 20:00:54: end of X-cor INFO @ Tue, 02 Aug 2022 20:00:54: #2 finished! INFO @ Tue, 02 Aug 2022 20:00:54: #2 predicted fragment length is 235 bps INFO @ Tue, 02 Aug 2022 20:00:54: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 02 Aug 2022 20:00:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10_model.r WARNING @ Tue, 02 Aug 2022 20:00:54: #2 Since the d (235) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 20:00:54: #2 You may need to consider one of the other alternative d(s): 235 WARNING @ Tue, 02 Aug 2022 20:00:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 20:00:54: #3 Call peaks... INFO @ Tue, 02 Aug 2022 20:00:54: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 20:00:55: #2 number of paired peaks: 199 WARNING @ Tue, 02 Aug 2022 20:00:55: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 02 Aug 2022 20:00:55: start model_add_line... INFO @ Tue, 02 Aug 2022 20:00:56: start X-correlation... INFO @ Tue, 02 Aug 2022 20:00:56: end of X-cor INFO @ Tue, 02 Aug 2022 20:00:56: #2 finished! INFO @ Tue, 02 Aug 2022 20:00:56: #2 predicted fragment length is 235 bps INFO @ Tue, 02 Aug 2022 20:00:56: #2 alternative fragment length(s) may be 235 bps INFO @ Tue, 02 Aug 2022 20:00:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20_model.r WARNING @ Tue, 02 Aug 2022 20:00:56: #2 Since the d (235) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 20:00:56: #2 You may need to consider one of the other alternative d(s): 235 WARNING @ Tue, 02 Aug 2022 20:00:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 20:00:56: #3 Call peaks... INFO @ Tue, 02 Aug 2022 20:00:56: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 20:01:09: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 20:01:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05_peaks.xls INFO @ Tue, 02 Aug 2022 20:01:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 20:01:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.05_summits.bed INFO @ Tue, 02 Aug 2022 20:01:44: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4370 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 20:01:59: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 20:02:00: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 20:02:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20_peaks.xls INFO @ Tue, 02 Aug 2022 20:02:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 20:02:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.20_summits.bed INFO @ Tue, 02 Aug 2022 20:02:33: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1321 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 20:02:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10_peaks.xls INFO @ Tue, 02 Aug 2022 20:02:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 20:02:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499368/SRX11499368.10_summits.bed INFO @ Tue, 02 Aug 2022 20:02:35: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2684 records, 4 fields): 7 millis CompletedMACS2peakCalling