Job ID = 16439696 SRX = SRX11499367 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11083998 spots for SRR15192724/SRR15192724.sra Written 11083998 spots for SRR15192724/SRR15192724.sra Read 14990321 spots for SRR15192725/SRR15192725.sra Written 14990321 spots for SRR15192725/SRR15192725.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440896 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:59:36 26074319 reads; of these: 26074319 (100.00%) were paired; of these: 3980899 (15.27%) aligned concordantly 0 times 17136553 (65.72%) aligned concordantly exactly 1 time 4956867 (19.01%) aligned concordantly >1 times ---- 3980899 pairs aligned concordantly 0 times; of these: 2003719 (50.33%) aligned discordantly 1 time ---- 1977180 pairs aligned 0 times concordantly or discordantly; of these: 3954360 mates make up the pairs; of these: 1731410 (43.78%) aligned 0 times 983545 (24.87%) aligned exactly 1 time 1239405 (31.34%) aligned >1 times 96.68% overall alignment rate Time searching: 01:59:36 Overall time: 01:59:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2623526 / 21711607 = 0.1208 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:38:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:38:44: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:38:44: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:39:00: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:39:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:39:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:39:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:39:15: 2000000 INFO @ Tue, 02 Aug 2022 18:39:28: 1000000 INFO @ Tue, 02 Aug 2022 18:39:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:39:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:39:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:39:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:39:44: 2000000 INFO @ Tue, 02 Aug 2022 18:39:47: 4000000 INFO @ Tue, 02 Aug 2022 18:40:01: 3000000 INFO @ Tue, 02 Aug 2022 18:40:03: 1000000 INFO @ Tue, 02 Aug 2022 18:40:03: 5000000 INFO @ Tue, 02 Aug 2022 18:40:18: 4000000 INFO @ Tue, 02 Aug 2022 18:40:21: 6000000 INFO @ Tue, 02 Aug 2022 18:40:23: 2000000 INFO @ Tue, 02 Aug 2022 18:40:36: 5000000 INFO @ Tue, 02 Aug 2022 18:40:38: 7000000 INFO @ Tue, 02 Aug 2022 18:40:42: 3000000 INFO @ Tue, 02 Aug 2022 18:40:54: 6000000 INFO @ Tue, 02 Aug 2022 18:40:56: 8000000 INFO @ Tue, 02 Aug 2022 18:41:02: 4000000 INFO @ Tue, 02 Aug 2022 18:41:12: 7000000 INFO @ Tue, 02 Aug 2022 18:41:14: 9000000 INFO @ Tue, 02 Aug 2022 18:41:21: 5000000 INFO @ Tue, 02 Aug 2022 18:41:30: 8000000 INFO @ Tue, 02 Aug 2022 18:41:32: 10000000 INFO @ Tue, 02 Aug 2022 18:41:41: 6000000 INFO @ Tue, 02 Aug 2022 18:41:48: 9000000 INFO @ Tue, 02 Aug 2022 18:41:49: 11000000 INFO @ Tue, 02 Aug 2022 18:42:01: 7000000 INFO @ Tue, 02 Aug 2022 18:42:06: 10000000 INFO @ Tue, 02 Aug 2022 18:42:06: 12000000 INFO @ Tue, 02 Aug 2022 18:42:22: 8000000 INFO @ Tue, 02 Aug 2022 18:42:24: 13000000 INFO @ Tue, 02 Aug 2022 18:42:25: 11000000 INFO @ Tue, 02 Aug 2022 18:42:43: 9000000 INFO @ Tue, 02 Aug 2022 18:42:43: 12000000 INFO @ Tue, 02 Aug 2022 18:42:43: 14000000 INFO @ Tue, 02 Aug 2022 18:43:01: 13000000 INFO @ Tue, 02 Aug 2022 18:43:01: 15000000 INFO @ Tue, 02 Aug 2022 18:43:04: 10000000 INFO @ Tue, 02 Aug 2022 18:43:18: 14000000 INFO @ Tue, 02 Aug 2022 18:43:20: 16000000 INFO @ Tue, 02 Aug 2022 18:43:24: 11000000 INFO @ Tue, 02 Aug 2022 18:43:36: 15000000 INFO @ Tue, 02 Aug 2022 18:43:38: 17000000 INFO @ Tue, 02 Aug 2022 18:43:45: 12000000 INFO @ Tue, 02 Aug 2022 18:43:53: 16000000 INFO @ Tue, 02 Aug 2022 18:43:57: 18000000 INFO @ Tue, 02 Aug 2022 18:44:05: 13000000 INFO @ Tue, 02 Aug 2022 18:44:11: 17000000 INFO @ Tue, 02 Aug 2022 18:44:16: 19000000 INFO @ Tue, 02 Aug 2022 18:44:26: 14000000 INFO @ Tue, 02 Aug 2022 18:44:28: 18000000 INFO @ Tue, 02 Aug 2022 18:44:34: 20000000 INFO @ Tue, 02 Aug 2022 18:44:44: 19000000 INFO @ Tue, 02 Aug 2022 18:44:45: 15000000 INFO @ Tue, 02 Aug 2022 18:44:49: 21000000 INFO @ Tue, 02 Aug 2022 18:45:01: 20000000 INFO @ Tue, 02 Aug 2022 18:45:04: 16000000 INFO @ Tue, 02 Aug 2022 18:45:05: 22000000 INFO @ Tue, 02 Aug 2022 18:45:15: 21000000 INFO @ Tue, 02 Aug 2022 18:45:21: 23000000 INFO @ Tue, 02 Aug 2022 18:45:24: 17000000 INFO @ Tue, 02 Aug 2022 18:45:29: 22000000 INFO @ Tue, 02 Aug 2022 18:45:37: 24000000 INFO @ Tue, 02 Aug 2022 18:45:43: 18000000 INFO @ Tue, 02 Aug 2022 18:45:44: 23000000 INFO @ Tue, 02 Aug 2022 18:45:52: 25000000 INFO @ Tue, 02 Aug 2022 18:45:58: 24000000 INFO @ Tue, 02 Aug 2022 18:46:03: 19000000 INFO @ Tue, 02 Aug 2022 18:46:08: 26000000 INFO @ Tue, 02 Aug 2022 18:46:12: 25000000 INFO @ Tue, 02 Aug 2022 18:46:22: 20000000 INFO @ Tue, 02 Aug 2022 18:46:23: 27000000 INFO @ Tue, 02 Aug 2022 18:46:27: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:46:39: 28000000 INFO @ Tue, 02 Aug 2022 18:46:42: 21000000 INFO @ Tue, 02 Aug 2022 18:46:42: 27000000 INFO @ Tue, 02 Aug 2022 18:46:54: 29000000 INFO @ Tue, 02 Aug 2022 18:46:56: 28000000 INFO @ Tue, 02 Aug 2022 18:47:00: 22000000 INFO @ Tue, 02 Aug 2022 18:47:10: 30000000 INFO @ Tue, 02 Aug 2022 18:47:10: 29000000 INFO @ Tue, 02 Aug 2022 18:47:19: 23000000 INFO @ Tue, 02 Aug 2022 18:47:24: 30000000 INFO @ Tue, 02 Aug 2022 18:47:26: 31000000 INFO @ Tue, 02 Aug 2022 18:47:38: 24000000 INFO @ Tue, 02 Aug 2022 18:47:39: 31000000 INFO @ Tue, 02 Aug 2022 18:47:42: 32000000 INFO @ Tue, 02 Aug 2022 18:47:53: 32000000 INFO @ Tue, 02 Aug 2022 18:47:57: 25000000 INFO @ Tue, 02 Aug 2022 18:47:59: 33000000 INFO @ Tue, 02 Aug 2022 18:48:07: 33000000 INFO @ Tue, 02 Aug 2022 18:48:15: 34000000 INFO @ Tue, 02 Aug 2022 18:48:16: 26000000 INFO @ Tue, 02 Aug 2022 18:48:21: 34000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:48:31: 35000000 INFO @ Tue, 02 Aug 2022 18:48:34: 27000000 INFO @ Tue, 02 Aug 2022 18:48:36: 35000000 INFO @ Tue, 02 Aug 2022 18:48:47: 36000000 INFO @ Tue, 02 Aug 2022 18:48:51: 36000000 INFO @ Tue, 02 Aug 2022 18:48:53: 28000000 INFO @ Tue, 02 Aug 2022 18:49:03: 37000000 INFO @ Tue, 02 Aug 2022 18:49:05: 37000000 INFO @ Tue, 02 Aug 2022 18:49:12: 29000000 INFO @ Tue, 02 Aug 2022 18:49:20: 38000000 INFO @ Tue, 02 Aug 2022 18:49:20: 38000000 INFO @ Tue, 02 Aug 2022 18:49:30: 30000000 INFO @ Tue, 02 Aug 2022 18:49:35: 39000000 INFO @ Tue, 02 Aug 2022 18:49:39: 39000000 INFO @ Tue, 02 Aug 2022 18:49:47: 31000000 INFO @ Tue, 02 Aug 2022 18:49:52: 40000000 INFO @ Tue, 02 Aug 2022 18:49:57: 40000000 INFO @ Tue, 02 Aug 2022 18:50:04: 32000000 INFO @ Tue, 02 Aug 2022 18:50:08: 41000000 INFO @ Tue, 02 Aug 2022 18:50:15: 41000000 INFO @ Tue, 02 Aug 2022 18:50:19: 33000000 INFO @ Tue, 02 Aug 2022 18:50:24: 42000000 INFO @ Tue, 02 Aug 2022 18:50:31: 42000000 INFO @ Tue, 02 Aug 2022 18:50:35: 34000000 INFO @ Tue, 02 Aug 2022 18:50:40: 43000000 INFO @ Tue, 02 Aug 2022 18:50:48: 43000000 INFO @ Tue, 02 Aug 2022 18:50:50: 35000000 INFO @ Tue, 02 Aug 2022 18:50:56: 44000000 INFO @ Tue, 02 Aug 2022 18:51:05: 36000000 INFO @ Tue, 02 Aug 2022 18:51:05: 44000000 INFO @ Tue, 02 Aug 2022 18:51:12: 45000000 INFO @ Tue, 02 Aug 2022 18:51:15: #1 tag size is determined as 141 bps INFO @ Tue, 02 Aug 2022 18:51:15: #1 tag size = 141 INFO @ Tue, 02 Aug 2022 18:51:15: #1 total tags in treatment: 19593888 INFO @ Tue, 02 Aug 2022 18:51:15: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:51:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:51:15: #1 tags after filtering in treatment: 17475589 INFO @ Tue, 02 Aug 2022 18:51:15: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 02 Aug 2022 18:51:15: #1 finished! INFO @ Tue, 02 Aug 2022 18:51:15: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:51:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:51:17: #2 number of paired peaks: 748 WARNING @ Tue, 02 Aug 2022 18:51:17: Fewer paired peaks (748) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 748 pairs to build model! INFO @ Tue, 02 Aug 2022 18:51:17: start model_add_line... INFO @ Tue, 02 Aug 2022 18:51:17: start X-correlation... INFO @ Tue, 02 Aug 2022 18:51:17: end of X-cor INFO @ Tue, 02 Aug 2022 18:51:17: #2 finished! INFO @ Tue, 02 Aug 2022 18:51:17: #2 predicted fragment length is 253 bps INFO @ Tue, 02 Aug 2022 18:51:17: #2 alternative fragment length(s) may be 253 bps INFO @ Tue, 02 Aug 2022 18:51:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10_model.r WARNING @ Tue, 02 Aug 2022 18:51:17: #2 Since the d (253) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:51:17: #2 You may need to consider one of the other alternative d(s): 253 WARNING @ Tue, 02 Aug 2022 18:51:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:51:17: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:51:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:51:19: 37000000 INFO @ Tue, 02 Aug 2022 18:51:22: 45000000 INFO @ Tue, 02 Aug 2022 18:51:25: #1 tag size is determined as 141 bps INFO @ Tue, 02 Aug 2022 18:51:25: #1 tag size = 141 INFO @ Tue, 02 Aug 2022 18:51:25: #1 total tags in treatment: 19593888 INFO @ Tue, 02 Aug 2022 18:51:25: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:51:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:51:26: #1 tags after filtering in treatment: 17475589 INFO @ Tue, 02 Aug 2022 18:51:26: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 02 Aug 2022 18:51:26: #1 finished! INFO @ Tue, 02 Aug 2022 18:51:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:51:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:51:27: #2 number of paired peaks: 748 WARNING @ Tue, 02 Aug 2022 18:51:27: Fewer paired peaks (748) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 748 pairs to build model! INFO @ Tue, 02 Aug 2022 18:51:27: start model_add_line... INFO @ Tue, 02 Aug 2022 18:51:27: start X-correlation... INFO @ Tue, 02 Aug 2022 18:51:27: end of X-cor INFO @ Tue, 02 Aug 2022 18:51:27: #2 finished! INFO @ Tue, 02 Aug 2022 18:51:27: #2 predicted fragment length is 253 bps INFO @ Tue, 02 Aug 2022 18:51:27: #2 alternative fragment length(s) may be 253 bps INFO @ Tue, 02 Aug 2022 18:51:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05_model.r WARNING @ Tue, 02 Aug 2022 18:51:27: #2 Since the d (253) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:51:27: #2 You may need to consider one of the other alternative d(s): 253 WARNING @ Tue, 02 Aug 2022 18:51:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:51:27: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:51:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:51:33: 38000000 INFO @ Tue, 02 Aug 2022 18:51:46: 39000000 INFO @ Tue, 02 Aug 2022 18:51:59: 40000000 INFO @ Tue, 02 Aug 2022 18:52:06: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:52:12: 41000000 INFO @ Tue, 02 Aug 2022 18:52:16: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:52:24: 42000000 INFO @ Tue, 02 Aug 2022 18:52:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10_peaks.xls INFO @ Tue, 02 Aug 2022 18:52:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:52:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.10_summits.bed INFO @ Tue, 02 Aug 2022 18:52:30: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (2517 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:52:37: 43000000 INFO @ Tue, 02 Aug 2022 18:52:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05_peaks.xls INFO @ Tue, 02 Aug 2022 18:52:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:52:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.05_summits.bed INFO @ Tue, 02 Aug 2022 18:52:40: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (3881 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:52:49: 44000000 INFO @ Tue, 02 Aug 2022 18:53:01: 45000000 INFO @ Tue, 02 Aug 2022 18:53:04: #1 tag size is determined as 141 bps INFO @ Tue, 02 Aug 2022 18:53:04: #1 tag size = 141 INFO @ Tue, 02 Aug 2022 18:53:04: #1 total tags in treatment: 19593888 INFO @ Tue, 02 Aug 2022 18:53:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:53:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:53:04: #1 tags after filtering in treatment: 17475589 INFO @ Tue, 02 Aug 2022 18:53:04: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 02 Aug 2022 18:53:04: #1 finished! INFO @ Tue, 02 Aug 2022 18:53:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:53:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:53:06: #2 number of paired peaks: 748 WARNING @ Tue, 02 Aug 2022 18:53:06: Fewer paired peaks (748) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 748 pairs to build model! INFO @ Tue, 02 Aug 2022 18:53:06: start model_add_line... INFO @ Tue, 02 Aug 2022 18:53:06: start X-correlation... INFO @ Tue, 02 Aug 2022 18:53:06: end of X-cor INFO @ Tue, 02 Aug 2022 18:53:06: #2 finished! INFO @ Tue, 02 Aug 2022 18:53:06: #2 predicted fragment length is 253 bps INFO @ Tue, 02 Aug 2022 18:53:06: #2 alternative fragment length(s) may be 253 bps INFO @ Tue, 02 Aug 2022 18:53:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20_model.r WARNING @ Tue, 02 Aug 2022 18:53:06: #2 Since the d (253) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 18:53:06: #2 You may need to consider one of the other alternative d(s): 253 WARNING @ Tue, 02 Aug 2022 18:53:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 18:53:06: #3 Call peaks... INFO @ Tue, 02 Aug 2022 18:53:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 18:53:55: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 18:54:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20_peaks.xls INFO @ Tue, 02 Aug 2022 18:54:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 18:54:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11499367/SRX11499367.20_summits.bed INFO @ Tue, 02 Aug 2022 18:54:18: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1491 records, 4 fields): 5 millis CompletedMACS2peakCalling