Job ID = 16439692 SRX = SRX11499363 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9584765 spots for SRR15192716/SRR15192716.sra Written 9584765 spots for SRR15192716/SRR15192716.sra Read 17605842 spots for SRR15192717/SRR15192717.sra Written 17605842 spots for SRR15192717/SRR15192717.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440996 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:20:42 27190607 reads; of these: 27190607 (100.00%) were paired; of these: 4369551 (16.07%) aligned concordantly 0 times 16884446 (62.10%) aligned concordantly exactly 1 time 5936610 (21.83%) aligned concordantly >1 times ---- 4369551 pairs aligned concordantly 0 times; of these: 2010449 (46.01%) aligned discordantly 1 time ---- 2359102 pairs aligned 0 times concordantly or discordantly; of these: 4718204 mates make up the pairs; of these: 2251142 (47.71%) aligned 0 times 1008767 (21.38%) aligned exactly 1 time 1458295 (30.91%) aligned >1 times 95.86% overall alignment rate Time searching: 02:20:43 Overall time: 02:20:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2818218 / 22251455 = 0.1267 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:58:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:58:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:58:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:58:23: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:58:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:58:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:58:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:58:39: 2000000 INFO @ Tue, 02 Aug 2022 18:58:53: 1000000 INFO @ Tue, 02 Aug 2022 18:58:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:59:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:59:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:59:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:59:09: 2000000 INFO @ Tue, 02 Aug 2022 18:59:13: 4000000 INFO @ Tue, 02 Aug 2022 18:59:23: 3000000 INFO @ Tue, 02 Aug 2022 18:59:25: 1000000 INFO @ Tue, 02 Aug 2022 18:59:30: 5000000 INFO @ Tue, 02 Aug 2022 18:59:39: 4000000 INFO @ Tue, 02 Aug 2022 18:59:42: 2000000 INFO @ Tue, 02 Aug 2022 18:59:47: 6000000 INFO @ Tue, 02 Aug 2022 18:59:54: 5000000 INFO @ Tue, 02 Aug 2022 18:59:59: 3000000 INFO @ Tue, 02 Aug 2022 19:00:03: 7000000 INFO @ Tue, 02 Aug 2022 19:00:09: 6000000 INFO @ Tue, 02 Aug 2022 19:00:16: 4000000 INFO @ Tue, 02 Aug 2022 19:00:20: 8000000 INFO @ Tue, 02 Aug 2022 19:00:24: 7000000 INFO @ Tue, 02 Aug 2022 19:00:33: 5000000 INFO @ Tue, 02 Aug 2022 19:00:37: 9000000 INFO @ Tue, 02 Aug 2022 19:00:39: 8000000 INFO @ Tue, 02 Aug 2022 19:00:48: 6000000 INFO @ Tue, 02 Aug 2022 19:00:54: 9000000 INFO @ Tue, 02 Aug 2022 19:00:54: 10000000 INFO @ Tue, 02 Aug 2022 19:01:04: 7000000 INFO @ Tue, 02 Aug 2022 19:01:09: 10000000 INFO @ Tue, 02 Aug 2022 19:01:12: 11000000 INFO @ Tue, 02 Aug 2022 19:01:21: 8000000 INFO @ Tue, 02 Aug 2022 19:01:24: 11000000 INFO @ Tue, 02 Aug 2022 19:01:29: 12000000 INFO @ Tue, 02 Aug 2022 19:01:36: 9000000 INFO @ Tue, 02 Aug 2022 19:01:39: 12000000 INFO @ Tue, 02 Aug 2022 19:01:47: 13000000 INFO @ Tue, 02 Aug 2022 19:01:51: 10000000 INFO @ Tue, 02 Aug 2022 19:01:54: 13000000 INFO @ Tue, 02 Aug 2022 19:02:03: 14000000 INFO @ Tue, 02 Aug 2022 19:02:07: 11000000 INFO @ Tue, 02 Aug 2022 19:02:10: 14000000 INFO @ Tue, 02 Aug 2022 19:02:19: 15000000 INFO @ Tue, 02 Aug 2022 19:02:23: 12000000 INFO @ Tue, 02 Aug 2022 19:02:25: 15000000 INFO @ Tue, 02 Aug 2022 19:02:36: 16000000 INFO @ Tue, 02 Aug 2022 19:02:40: 16000000 INFO @ Tue, 02 Aug 2022 19:02:40: 13000000 INFO @ Tue, 02 Aug 2022 19:02:53: 17000000 INFO @ Tue, 02 Aug 2022 19:02:55: 17000000 INFO @ Tue, 02 Aug 2022 19:02:57: 14000000 INFO @ Tue, 02 Aug 2022 19:03:10: 18000000 INFO @ Tue, 02 Aug 2022 19:03:10: 18000000 INFO @ Tue, 02 Aug 2022 19:03:12: 15000000 INFO @ Tue, 02 Aug 2022 19:03:26: 19000000 INFO @ Tue, 02 Aug 2022 19:03:27: 19000000 INFO @ Tue, 02 Aug 2022 19:03:28: 16000000 INFO @ Tue, 02 Aug 2022 19:03:41: 20000000 INFO @ Tue, 02 Aug 2022 19:03:43: 20000000 INFO @ Tue, 02 Aug 2022 19:03:45: 17000000 INFO @ Tue, 02 Aug 2022 19:03:56: 21000000 INFO @ Tue, 02 Aug 2022 19:03:58: 21000000 INFO @ Tue, 02 Aug 2022 19:04:02: 18000000 INFO @ Tue, 02 Aug 2022 19:04:11: 22000000 INFO @ Tue, 02 Aug 2022 19:04:13: 22000000 INFO @ Tue, 02 Aug 2022 19:04:18: 19000000 INFO @ Tue, 02 Aug 2022 19:04:26: 23000000 INFO @ Tue, 02 Aug 2022 19:04:29: 23000000 INFO @ Tue, 02 Aug 2022 19:04:34: 20000000 INFO @ Tue, 02 Aug 2022 19:04:41: 24000000 INFO @ Tue, 02 Aug 2022 19:04:44: 24000000 INFO @ Tue, 02 Aug 2022 19:04:51: 21000000 INFO @ Tue, 02 Aug 2022 19:04:57: 25000000 INFO @ Tue, 02 Aug 2022 19:05:00: 25000000 INFO @ Tue, 02 Aug 2022 19:05:08: 22000000 INFO @ Tue, 02 Aug 2022 19:05:11: 26000000 INFO @ Tue, 02 Aug 2022 19:05:15: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:05:24: 23000000 INFO @ Tue, 02 Aug 2022 19:05:26: 27000000 INFO @ Tue, 02 Aug 2022 19:05:31: 27000000 INFO @ Tue, 02 Aug 2022 19:05:41: 24000000 INFO @ Tue, 02 Aug 2022 19:05:41: 28000000 INFO @ Tue, 02 Aug 2022 19:05:46: 28000000 INFO @ Tue, 02 Aug 2022 19:05:55: 29000000 INFO @ Tue, 02 Aug 2022 19:05:57: 25000000 INFO @ Tue, 02 Aug 2022 19:06:01: 29000000 INFO @ Tue, 02 Aug 2022 19:06:10: 30000000 INFO @ Tue, 02 Aug 2022 19:06:13: 26000000 INFO @ Tue, 02 Aug 2022 19:06:16: 30000000 INFO @ Tue, 02 Aug 2022 19:06:25: 31000000 INFO @ Tue, 02 Aug 2022 19:06:30: 27000000 INFO @ Tue, 02 Aug 2022 19:06:32: 31000000 INFO @ Tue, 02 Aug 2022 19:06:40: 32000000 INFO @ Tue, 02 Aug 2022 19:06:46: 28000000 INFO @ Tue, 02 Aug 2022 19:06:47: 32000000 INFO @ Tue, 02 Aug 2022 19:06:57: 33000000 INFO @ Tue, 02 Aug 2022 19:07:03: 29000000 INFO @ Tue, 02 Aug 2022 19:07:04: 33000000 INFO @ Tue, 02 Aug 2022 19:07:13: 34000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:07:20: 34000000 INFO @ Tue, 02 Aug 2022 19:07:20: 30000000 INFO @ Tue, 02 Aug 2022 19:07:29: 35000000 INFO @ Tue, 02 Aug 2022 19:07:37: 31000000 INFO @ Tue, 02 Aug 2022 19:07:38: 35000000 INFO @ Tue, 02 Aug 2022 19:07:44: 36000000 INFO @ Tue, 02 Aug 2022 19:07:52: 32000000 INFO @ Tue, 02 Aug 2022 19:07:54: 36000000 INFO @ Tue, 02 Aug 2022 19:08:00: 37000000 INFO @ Tue, 02 Aug 2022 19:08:08: 33000000 INFO @ Tue, 02 Aug 2022 19:08:11: 37000000 INFO @ Tue, 02 Aug 2022 19:08:16: 38000000 INFO @ Tue, 02 Aug 2022 19:08:23: 34000000 INFO @ Tue, 02 Aug 2022 19:08:28: 38000000 INFO @ Tue, 02 Aug 2022 19:08:33: 39000000 INFO @ Tue, 02 Aug 2022 19:08:39: 35000000 INFO @ Tue, 02 Aug 2022 19:08:45: 39000000 INFO @ Tue, 02 Aug 2022 19:08:49: 40000000 INFO @ Tue, 02 Aug 2022 19:08:55: 36000000 INFO @ Tue, 02 Aug 2022 19:09:03: 40000000 INFO @ Tue, 02 Aug 2022 19:09:06: 41000000 INFO @ Tue, 02 Aug 2022 19:09:11: 37000000 INFO @ Tue, 02 Aug 2022 19:09:21: 41000000 INFO @ Tue, 02 Aug 2022 19:09:23: 42000000 INFO @ Tue, 02 Aug 2022 19:09:27: 38000000 INFO @ Tue, 02 Aug 2022 19:09:37: 42000000 INFO @ Tue, 02 Aug 2022 19:09:38: 43000000 INFO @ Tue, 02 Aug 2022 19:09:43: 39000000 INFO @ Tue, 02 Aug 2022 19:09:54: 43000000 INFO @ Tue, 02 Aug 2022 19:09:56: 44000000 INFO @ Tue, 02 Aug 2022 19:09:59: 40000000 INFO @ Tue, 02 Aug 2022 19:10:12: 45000000 INFO @ Tue, 02 Aug 2022 19:10:12: 44000000 INFO @ Tue, 02 Aug 2022 19:10:14: 41000000 INFO @ Tue, 02 Aug 2022 19:10:30: 45000000 INFO @ Tue, 02 Aug 2022 19:10:30: 46000000 INFO @ Tue, 02 Aug 2022 19:10:30: 42000000 INFO @ Tue, 02 Aug 2022 19:10:39: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 19:10:39: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 19:10:39: #1 total tags in treatment: 20120610 INFO @ Tue, 02 Aug 2022 19:10:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:10:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:10:39: #1 tags after filtering in treatment: 18741332 INFO @ Tue, 02 Aug 2022 19:10:39: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:10:39: #1 finished! INFO @ Tue, 02 Aug 2022 19:10:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:10:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:10:41: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 19:10:41: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 19:10:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:10:45: 43000000 INFO @ Tue, 02 Aug 2022 19:10:48: 46000000 INFO @ Tue, 02 Aug 2022 19:10:56: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 19:10:56: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 19:10:56: #1 total tags in treatment: 20120610 INFO @ Tue, 02 Aug 2022 19:10:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:10:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:10:57: #1 tags after filtering in treatment: 18741332 INFO @ Tue, 02 Aug 2022 19:10:57: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:10:57: #1 finished! INFO @ Tue, 02 Aug 2022 19:10:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:10:58: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 19:10:58: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 19:10:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:11:01: 44000000 INFO @ Tue, 02 Aug 2022 19:11:17: 45000000 INFO @ Tue, 02 Aug 2022 19:11:32: 46000000 INFO @ Tue, 02 Aug 2022 19:11:40: #1 tag size is determined as 130 bps INFO @ Tue, 02 Aug 2022 19:11:40: #1 tag size = 130 INFO @ Tue, 02 Aug 2022 19:11:40: #1 total tags in treatment: 20120610 INFO @ Tue, 02 Aug 2022 19:11:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:11:40: #1 tags after filtering in treatment: 18741332 INFO @ Tue, 02 Aug 2022 19:11:40: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:11:40: #1 finished! INFO @ Tue, 02 Aug 2022 19:11:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:11:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:11:42: #2 number of paired peaks: 56 WARNING @ Tue, 02 Aug 2022 19:11:42: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 19:11:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499363/SRX11499363.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling