Job ID = 16439689 SRX = SRX11499360 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12596739 spots for SRR15192710/SRR15192710.sra Written 12596739 spots for SRR15192710/SRR15192710.sra Read 17197479 spots for SRR15192711/SRR15192711.sra Written 17197479 spots for SRR15192711/SRR15192711.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440753 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:46:58 29794218 reads; of these: 29794218 (100.00%) were paired; of these: 4831738 (16.22%) aligned concordantly 0 times 18156213 (60.94%) aligned concordantly exactly 1 time 6806267 (22.84%) aligned concordantly >1 times ---- 4831738 pairs aligned concordantly 0 times; of these: 2349696 (48.63%) aligned discordantly 1 time ---- 2482042 pairs aligned 0 times concordantly or discordantly; of these: 4964084 mates make up the pairs; of these: 1946214 (39.21%) aligned 0 times 1139429 (22.95%) aligned exactly 1 time 1878441 (37.84%) aligned >1 times 96.73% overall alignment rate Time searching: 01:46:58 Overall time: 01:46:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3116087 / 24709445 = 0.1261 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:12:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:12:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:12:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:13:08: 1000000 INFO @ Tue, 02 Aug 2022 18:13:17: 2000000 INFO @ Tue, 02 Aug 2022 18:13:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:13:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:13:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:13:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:13:35: 4000000 INFO @ Tue, 02 Aug 2022 18:13:37: 1000000 INFO @ Tue, 02 Aug 2022 18:13:45: 5000000 INFO @ Tue, 02 Aug 2022 18:13:46: 2000000 INFO @ Tue, 02 Aug 2022 18:13:55: 6000000 INFO @ Tue, 02 Aug 2022 18:13:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:13:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:13:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:13:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:14:05: 4000000 INFO @ Tue, 02 Aug 2022 18:14:05: 7000000 INFO @ Tue, 02 Aug 2022 18:14:07: 1000000 INFO @ Tue, 02 Aug 2022 18:14:15: 5000000 INFO @ Tue, 02 Aug 2022 18:14:15: 8000000 INFO @ Tue, 02 Aug 2022 18:14:17: 2000000 INFO @ Tue, 02 Aug 2022 18:14:24: 6000000 INFO @ Tue, 02 Aug 2022 18:14:25: 9000000 INFO @ Tue, 02 Aug 2022 18:14:26: 3000000 INFO @ Tue, 02 Aug 2022 18:14:34: 7000000 INFO @ Tue, 02 Aug 2022 18:14:35: 10000000 INFO @ Tue, 02 Aug 2022 18:14:36: 4000000 INFO @ Tue, 02 Aug 2022 18:14:43: 8000000 INFO @ Tue, 02 Aug 2022 18:14:45: 5000000 INFO @ Tue, 02 Aug 2022 18:14:45: 11000000 INFO @ Tue, 02 Aug 2022 18:14:52: 9000000 INFO @ Tue, 02 Aug 2022 18:14:55: 6000000 INFO @ Tue, 02 Aug 2022 18:14:55: 12000000 INFO @ Tue, 02 Aug 2022 18:15:02: 10000000 INFO @ Tue, 02 Aug 2022 18:15:04: 7000000 INFO @ Tue, 02 Aug 2022 18:15:05: 13000000 INFO @ Tue, 02 Aug 2022 18:15:11: 11000000 INFO @ Tue, 02 Aug 2022 18:15:13: 8000000 INFO @ Tue, 02 Aug 2022 18:15:14: 14000000 INFO @ Tue, 02 Aug 2022 18:15:21: 12000000 INFO @ Tue, 02 Aug 2022 18:15:23: 9000000 INFO @ Tue, 02 Aug 2022 18:15:24: 15000000 INFO @ Tue, 02 Aug 2022 18:15:30: 13000000 INFO @ Tue, 02 Aug 2022 18:15:32: 10000000 INFO @ Tue, 02 Aug 2022 18:15:33: 16000000 INFO @ Tue, 02 Aug 2022 18:15:40: 14000000 INFO @ Tue, 02 Aug 2022 18:15:42: 11000000 INFO @ Tue, 02 Aug 2022 18:15:43: 17000000 INFO @ Tue, 02 Aug 2022 18:15:49: 15000000 INFO @ Tue, 02 Aug 2022 18:15:52: 12000000 INFO @ Tue, 02 Aug 2022 18:15:52: 18000000 INFO @ Tue, 02 Aug 2022 18:15:59: 16000000 INFO @ Tue, 02 Aug 2022 18:16:01: 13000000 INFO @ Tue, 02 Aug 2022 18:16:02: 19000000 INFO @ Tue, 02 Aug 2022 18:16:09: 17000000 INFO @ Tue, 02 Aug 2022 18:16:11: 14000000 INFO @ Tue, 02 Aug 2022 18:16:12: 20000000 INFO @ Tue, 02 Aug 2022 18:16:18: 18000000 INFO @ Tue, 02 Aug 2022 18:16:21: 15000000 INFO @ Tue, 02 Aug 2022 18:16:21: 21000000 INFO @ Tue, 02 Aug 2022 18:16:28: 19000000 INFO @ Tue, 02 Aug 2022 18:16:30: 16000000 INFO @ Tue, 02 Aug 2022 18:16:31: 22000000 INFO @ Tue, 02 Aug 2022 18:16:37: 20000000 INFO @ Tue, 02 Aug 2022 18:16:40: 17000000 INFO @ Tue, 02 Aug 2022 18:16:40: 23000000 INFO @ Tue, 02 Aug 2022 18:16:47: 21000000 INFO @ Tue, 02 Aug 2022 18:16:49: 18000000 INFO @ Tue, 02 Aug 2022 18:16:49: 24000000 INFO @ Tue, 02 Aug 2022 18:16:56: 22000000 INFO @ Tue, 02 Aug 2022 18:16:59: 19000000 INFO @ Tue, 02 Aug 2022 18:16:59: 25000000 INFO @ Tue, 02 Aug 2022 18:17:05: 23000000 INFO @ Tue, 02 Aug 2022 18:17:08: 20000000 INFO @ Tue, 02 Aug 2022 18:17:08: 26000000 INFO @ Tue, 02 Aug 2022 18:17:15: 24000000 INFO @ Tue, 02 Aug 2022 18:17:18: 21000000 INFO @ Tue, 02 Aug 2022 18:17:18: 27000000 INFO @ Tue, 02 Aug 2022 18:17:24: 25000000 INFO @ Tue, 02 Aug 2022 18:17:27: 22000000 INFO @ Tue, 02 Aug 2022 18:17:27: 28000000 INFO @ Tue, 02 Aug 2022 18:17:34: 26000000 INFO @ Tue, 02 Aug 2022 18:17:36: 23000000 INFO @ Tue, 02 Aug 2022 18:17:36: 29000000 INFO @ Tue, 02 Aug 2022 18:17:43: 27000000 INFO @ Tue, 02 Aug 2022 18:17:46: 24000000 INFO @ Tue, 02 Aug 2022 18:17:46: 30000000 INFO @ Tue, 02 Aug 2022 18:17:53: 28000000 INFO @ Tue, 02 Aug 2022 18:17:56: 31000000 INFO @ Tue, 02 Aug 2022 18:17:56: 25000000 INFO @ Tue, 02 Aug 2022 18:18:03: 29000000 INFO @ Tue, 02 Aug 2022 18:18:05: 32000000 INFO @ Tue, 02 Aug 2022 18:18:05: 26000000 INFO @ Tue, 02 Aug 2022 18:18:12: 30000000 INFO @ Tue, 02 Aug 2022 18:18:15: 27000000 INFO @ Tue, 02 Aug 2022 18:18:15: 33000000 INFO @ Tue, 02 Aug 2022 18:18:21: 31000000 INFO @ Tue, 02 Aug 2022 18:18:24: 28000000 INFO @ Tue, 02 Aug 2022 18:18:25: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:18:31: 32000000 INFO @ Tue, 02 Aug 2022 18:18:34: 29000000 INFO @ Tue, 02 Aug 2022 18:18:34: 35000000 INFO @ Tue, 02 Aug 2022 18:18:40: 33000000 INFO @ Tue, 02 Aug 2022 18:18:43: 30000000 INFO @ Tue, 02 Aug 2022 18:18:44: 36000000 INFO @ Tue, 02 Aug 2022 18:18:50: 34000000 INFO @ Tue, 02 Aug 2022 18:18:53: 31000000 INFO @ Tue, 02 Aug 2022 18:18:54: 37000000 INFO @ Tue, 02 Aug 2022 18:18:59: 35000000 INFO @ Tue, 02 Aug 2022 18:19:02: 32000000 INFO @ Tue, 02 Aug 2022 18:19:03: 38000000 INFO @ Tue, 02 Aug 2022 18:19:08: 36000000 INFO @ Tue, 02 Aug 2022 18:19:11: 33000000 INFO @ Tue, 02 Aug 2022 18:19:13: 39000000 INFO @ Tue, 02 Aug 2022 18:19:18: 37000000 INFO @ Tue, 02 Aug 2022 18:19:21: 34000000 INFO @ Tue, 02 Aug 2022 18:19:23: 40000000 INFO @ Tue, 02 Aug 2022 18:19:27: 38000000 INFO @ Tue, 02 Aug 2022 18:19:30: 35000000 INFO @ Tue, 02 Aug 2022 18:19:32: 41000000 INFO @ Tue, 02 Aug 2022 18:19:37: 39000000 INFO @ Tue, 02 Aug 2022 18:19:39: 36000000 INFO @ Tue, 02 Aug 2022 18:19:42: 42000000 INFO @ Tue, 02 Aug 2022 18:19:46: 40000000 INFO @ Tue, 02 Aug 2022 18:19:49: 37000000 INFO @ Tue, 02 Aug 2022 18:19:52: 43000000 INFO @ Tue, 02 Aug 2022 18:19:56: 41000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:19:58: 38000000 INFO @ Tue, 02 Aug 2022 18:20:01: 44000000 INFO @ Tue, 02 Aug 2022 18:20:05: 42000000 INFO @ Tue, 02 Aug 2022 18:20:08: 39000000 INFO @ Tue, 02 Aug 2022 18:20:11: 45000000 INFO @ Tue, 02 Aug 2022 18:20:14: 43000000 INFO @ Tue, 02 Aug 2022 18:20:17: 40000000 INFO @ Tue, 02 Aug 2022 18:20:20: 46000000 INFO @ Tue, 02 Aug 2022 18:20:24: 44000000 INFO @ Tue, 02 Aug 2022 18:20:26: 41000000 INFO @ Tue, 02 Aug 2022 18:20:29: 47000000 INFO @ Tue, 02 Aug 2022 18:20:33: 45000000 INFO @ Tue, 02 Aug 2022 18:20:36: 42000000 INFO @ Tue, 02 Aug 2022 18:20:39: 48000000 INFO @ Tue, 02 Aug 2022 18:20:42: 46000000 INFO @ Tue, 02 Aug 2022 18:20:45: 43000000 INFO @ Tue, 02 Aug 2022 18:20:49: 49000000 INFO @ Tue, 02 Aug 2022 18:20:52: 47000000 INFO @ Tue, 02 Aug 2022 18:20:54: 44000000 INFO @ Tue, 02 Aug 2022 18:20:58: 50000000 INFO @ Tue, 02 Aug 2022 18:21:01: 48000000 INFO @ Tue, 02 Aug 2022 18:21:04: 45000000 INFO @ Tue, 02 Aug 2022 18:21:07: 51000000 INFO @ Tue, 02 Aug 2022 18:21:11: 49000000 INFO @ Tue, 02 Aug 2022 18:21:11: #1 tag size is determined as 129 bps INFO @ Tue, 02 Aug 2022 18:21:11: #1 tag size = 129 INFO @ Tue, 02 Aug 2022 18:21:11: #1 total tags in treatment: 21990366 INFO @ Tue, 02 Aug 2022 18:21:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:21:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:21:12: #1 tags after filtering in treatment: 20089458 INFO @ Tue, 02 Aug 2022 18:21:12: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:21:12: #1 finished! INFO @ Tue, 02 Aug 2022 18:21:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:21:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:21:13: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 18:21:13: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:21:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:21:13: 46000000 INFO @ Tue, 02 Aug 2022 18:21:20: 50000000 INFO @ Tue, 02 Aug 2022 18:21:23: 47000000 INFO @ Tue, 02 Aug 2022 18:21:29: 51000000 INFO @ Tue, 02 Aug 2022 18:21:32: 48000000 INFO @ Tue, 02 Aug 2022 18:21:33: #1 tag size is determined as 129 bps INFO @ Tue, 02 Aug 2022 18:21:33: #1 tag size = 129 INFO @ Tue, 02 Aug 2022 18:21:33: #1 total tags in treatment: 21990366 INFO @ Tue, 02 Aug 2022 18:21:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:21:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:21:34: #1 tags after filtering in treatment: 20089458 INFO @ Tue, 02 Aug 2022 18:21:34: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:21:34: #1 finished! INFO @ Tue, 02 Aug 2022 18:21:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:21:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:21:35: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 18:21:35: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:21:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:21:41: 49000000 INFO @ Tue, 02 Aug 2022 18:21:49: 50000000 INFO @ Tue, 02 Aug 2022 18:21:58: 51000000 INFO @ Tue, 02 Aug 2022 18:22:01: #1 tag size is determined as 129 bps INFO @ Tue, 02 Aug 2022 18:22:01: #1 tag size = 129 INFO @ Tue, 02 Aug 2022 18:22:01: #1 total tags in treatment: 21990366 INFO @ Tue, 02 Aug 2022 18:22:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:22:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:22:01: #1 tags after filtering in treatment: 20089458 INFO @ Tue, 02 Aug 2022 18:22:01: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 02 Aug 2022 18:22:01: #1 finished! INFO @ Tue, 02 Aug 2022 18:22:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:22:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:22:03: #2 number of paired peaks: 74 WARNING @ Tue, 02 Aug 2022 18:22:03: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:22:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX11499360/SRX11499360.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling