Job ID = 1293778 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,633,613 reads read : 17,633,613 reads written : 17,633,613 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:02 17633613 reads; of these: 17633613 (100.00%) were unpaired; of these: 125295 (0.71%) aligned 0 times 11929096 (67.65%) aligned exactly 1 time 5579222 (31.64%) aligned >1 times 99.29% overall alignment rate Time searching: 00:08:02 Overall time: 00:08:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3549107 / 17508318 = 0.2027 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:16: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:16: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:16: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:16: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:35:16: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:35:16: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:35:23: 1000000 INFO @ Mon, 03 Jun 2019 02:35:24: 1000000 INFO @ Mon, 03 Jun 2019 02:35:25: 1000000 INFO @ Mon, 03 Jun 2019 02:35:31: 2000000 INFO @ Mon, 03 Jun 2019 02:35:31: 2000000 INFO @ Mon, 03 Jun 2019 02:35:32: 2000000 INFO @ Mon, 03 Jun 2019 02:35:38: 3000000 INFO @ Mon, 03 Jun 2019 02:35:39: 3000000 INFO @ Mon, 03 Jun 2019 02:35:39: 3000000 INFO @ Mon, 03 Jun 2019 02:35:45: 4000000 INFO @ Mon, 03 Jun 2019 02:35:46: 4000000 INFO @ Mon, 03 Jun 2019 02:35:46: 4000000 INFO @ Mon, 03 Jun 2019 02:35:52: 5000000 INFO @ Mon, 03 Jun 2019 02:35:53: 5000000 INFO @ Mon, 03 Jun 2019 02:35:53: 5000000 INFO @ Mon, 03 Jun 2019 02:35:59: 6000000 INFO @ Mon, 03 Jun 2019 02:36:00: 6000000 INFO @ Mon, 03 Jun 2019 02:36:01: 6000000 INFO @ Mon, 03 Jun 2019 02:36:06: 7000000 INFO @ Mon, 03 Jun 2019 02:36:08: 7000000 INFO @ Mon, 03 Jun 2019 02:36:08: 7000000 INFO @ Mon, 03 Jun 2019 02:36:13: 8000000 INFO @ Mon, 03 Jun 2019 02:36:15: 8000000 INFO @ Mon, 03 Jun 2019 02:36:15: 8000000 INFO @ Mon, 03 Jun 2019 02:36:20: 9000000 INFO @ Mon, 03 Jun 2019 02:36:22: 9000000 INFO @ Mon, 03 Jun 2019 02:36:22: 9000000 INFO @ Mon, 03 Jun 2019 02:36:28: 10000000 INFO @ Mon, 03 Jun 2019 02:36:29: 10000000 INFO @ Mon, 03 Jun 2019 02:36:29: 10000000 INFO @ Mon, 03 Jun 2019 02:36:35: 11000000 INFO @ Mon, 03 Jun 2019 02:36:36: 11000000 INFO @ Mon, 03 Jun 2019 02:36:37: 11000000 INFO @ Mon, 03 Jun 2019 02:36:42: 12000000 INFO @ Mon, 03 Jun 2019 02:36:42: 12000000 INFO @ Mon, 03 Jun 2019 02:36:45: 12000000 INFO @ Mon, 03 Jun 2019 02:36:49: 13000000 INFO @ Mon, 03 Jun 2019 02:36:49: 13000000 INFO @ Mon, 03 Jun 2019 02:36:53: 13000000 INFO @ Mon, 03 Jun 2019 02:36:56: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:36:56: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:36:56: #1 total tags in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:36:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:36:56: #1 tags after filtering in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:36:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:36:56: #1 finished! INFO @ Mon, 03 Jun 2019 02:36:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:36:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:36:57: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:36:57: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:36:57: #1 total tags in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:36:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:36:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:36:57: #1 tags after filtering in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:36:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:36:57: #1 finished! INFO @ Mon, 03 Jun 2019 02:36:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:36:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:36:58: #2 number of paired peaks: 47 WARNING @ Mon, 03 Jun 2019 02:36:58: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:36:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:36:58: #2 number of paired peaks: 47 WARNING @ Mon, 03 Jun 2019 02:36:58: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:36:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:37:01: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:37:01: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:37:01: #1 total tags in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:37:01: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:37:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:37:01: #1 tags after filtering in treatment: 13959211 INFO @ Mon, 03 Jun 2019 02:37:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:37:01: #1 finished! INFO @ Mon, 03 Jun 2019 02:37:01: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:37:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:37:02: #2 number of paired peaks: 47 WARNING @ Mon, 03 Jun 2019 02:37:02: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:37:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113327/SRX113327.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。