Job ID = 1293777 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,354,925 reads read : 20,354,925 reads written : 20,354,925 spots read : 7,964,356 reads read : 7,964,356 reads written : 7,964,356 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:23 28319281 reads; of these: 28319281 (100.00%) were unpaired; of these: 1717484 (6.06%) aligned 0 times 21435078 (75.69%) aligned exactly 1 time 5166719 (18.24%) aligned >1 times 93.94% overall alignment rate Time searching: 00:08:23 Overall time: 00:08:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6225522 / 26601797 = 0.2340 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:33:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:33:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:33:52: 1000000 INFO @ Mon, 03 Jun 2019 02:33:55: 1000000 INFO @ Mon, 03 Jun 2019 02:33:56: 1000000 INFO @ Mon, 03 Jun 2019 02:34:00: 2000000 INFO @ Mon, 03 Jun 2019 02:34:06: 2000000 INFO @ Mon, 03 Jun 2019 02:34:07: 2000000 INFO @ Mon, 03 Jun 2019 02:34:09: 3000000 INFO @ Mon, 03 Jun 2019 02:34:17: 3000000 INFO @ Mon, 03 Jun 2019 02:34:17: 4000000 INFO @ Mon, 03 Jun 2019 02:34:17: 3000000 INFO @ Mon, 03 Jun 2019 02:34:25: 5000000 INFO @ Mon, 03 Jun 2019 02:34:27: 4000000 INFO @ Mon, 03 Jun 2019 02:34:28: 4000000 INFO @ Mon, 03 Jun 2019 02:34:33: 6000000 INFO @ Mon, 03 Jun 2019 02:34:37: 5000000 INFO @ Mon, 03 Jun 2019 02:34:39: 5000000 INFO @ Mon, 03 Jun 2019 02:34:42: 7000000 INFO @ Mon, 03 Jun 2019 02:34:48: 6000000 INFO @ Mon, 03 Jun 2019 02:34:50: 8000000 INFO @ Mon, 03 Jun 2019 02:34:51: 6000000 INFO @ Mon, 03 Jun 2019 02:34:58: 7000000 INFO @ Mon, 03 Jun 2019 02:34:59: 9000000 INFO @ Mon, 03 Jun 2019 02:35:02: 7000000 INFO @ Mon, 03 Jun 2019 02:35:07: 10000000 INFO @ Mon, 03 Jun 2019 02:35:09: 8000000 INFO @ Mon, 03 Jun 2019 02:35:13: 8000000 INFO @ Mon, 03 Jun 2019 02:35:15: 11000000 INFO @ Mon, 03 Jun 2019 02:35:19: 9000000 INFO @ Mon, 03 Jun 2019 02:35:23: 12000000 INFO @ Mon, 03 Jun 2019 02:35:24: 9000000 INFO @ Mon, 03 Jun 2019 02:35:29: 10000000 INFO @ Mon, 03 Jun 2019 02:35:32: 13000000 INFO @ Mon, 03 Jun 2019 02:35:35: 10000000 INFO @ Mon, 03 Jun 2019 02:35:39: 11000000 INFO @ Mon, 03 Jun 2019 02:35:40: 14000000 INFO @ Mon, 03 Jun 2019 02:35:46: 11000000 INFO @ Mon, 03 Jun 2019 02:35:48: 15000000 INFO @ Mon, 03 Jun 2019 02:35:50: 12000000 INFO @ Mon, 03 Jun 2019 02:35:56: 16000000 INFO @ Mon, 03 Jun 2019 02:35:57: 12000000 INFO @ Mon, 03 Jun 2019 02:36:00: 13000000 INFO @ Mon, 03 Jun 2019 02:36:05: 17000000 INFO @ Mon, 03 Jun 2019 02:36:08: 13000000 INFO @ Mon, 03 Jun 2019 02:36:10: 14000000 INFO @ Mon, 03 Jun 2019 02:36:14: 18000000 INFO @ Mon, 03 Jun 2019 02:36:19: 14000000 INFO @ Mon, 03 Jun 2019 02:36:20: 15000000 INFO @ Mon, 03 Jun 2019 02:36:24: 19000000 INFO @ Mon, 03 Jun 2019 02:36:29: 15000000 INFO @ Mon, 03 Jun 2019 02:36:31: 16000000 INFO @ Mon, 03 Jun 2019 02:36:32: 20000000 INFO @ Mon, 03 Jun 2019 02:36:35: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:36:35: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:36:35: #1 total tags in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:36:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:36:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:36:36: #1 tags after filtering in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:36:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:36:36: #1 finished! INFO @ Mon, 03 Jun 2019 02:36:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:36:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:36:37: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 02:36:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:36:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:36:40: 16000000 INFO @ Mon, 03 Jun 2019 02:36:42: 17000000 INFO @ Mon, 03 Jun 2019 02:36:52: 17000000 INFO @ Mon, 03 Jun 2019 02:36:52: 18000000 INFO @ Mon, 03 Jun 2019 02:37:02: 18000000 INFO @ Mon, 03 Jun 2019 02:37:03: 19000000 INFO @ Mon, 03 Jun 2019 02:37:13: 20000000 INFO @ Mon, 03 Jun 2019 02:37:13: 19000000 INFO @ Mon, 03 Jun 2019 02:37:17: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:37:17: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:37:17: #1 total tags in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:37:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:37:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:37:17: #1 tags after filtering in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:37:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:37:17: #1 finished! INFO @ Mon, 03 Jun 2019 02:37:17: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:37:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:37:19: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 02:37:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:37:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:37:23: 20000000 INFO @ Mon, 03 Jun 2019 02:37:27: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:37:27: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:37:27: #1 total tags in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:37:27: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:37:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:37:27: #1 tags after filtering in treatment: 20376275 INFO @ Mon, 03 Jun 2019 02:37:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:37:27: #1 finished! INFO @ Mon, 03 Jun 2019 02:37:27: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:37:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:37:29: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 02:37:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:37:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113326/SRX113326.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。