Job ID = 9158350 sra ファイルのダウンロード中... Completed: 1122762K bytes transferred in 13 seconds (674504K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 17486457 spots for /home/okishinya/chipatlas/results/dm3/SRX113317/SRR392929.sra Written 17486457 spots total Written 20194013 spots for /home/okishinya/chipatlas/results/dm3/SRX113317/SRR392928.sra Written 20194013 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:00 37680470 reads; of these: 37680470 (100.00%) were unpaired; of these: 1956432 (5.19%) aligned 0 times 27093171 (71.90%) aligned exactly 1 time 8630867 (22.91%) aligned >1 times 94.81% overall alignment rate Time searching: 00:13:00 Overall time: 00:13:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11194538 / 35724038 = 0.3134 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:37:19: # Command line: callpeak -t SRX113317.bam -f BAM -g dm -n SRX113317.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113317.10 # format = BAM # ChIP-seq file = ['SRX113317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:37:19: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:37:19: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:37:19: # Command line: callpeak -t SRX113317.bam -f BAM -g dm -n SRX113317.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113317.05 # format = BAM # ChIP-seq file = ['SRX113317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:37:19: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:37:19: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:37:19: # Command line: callpeak -t SRX113317.bam -f BAM -g dm -n SRX113317.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113317.20 # format = BAM # ChIP-seq file = ['SRX113317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:37:19: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:37:19: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:37:26: 1000000 INFO @ Tue, 27 Jun 2017 16:37:26: 1000000 INFO @ Tue, 27 Jun 2017 16:37:26: 1000000 INFO @ Tue, 27 Jun 2017 16:37:33: 2000000 INFO @ Tue, 27 Jun 2017 16:37:33: 2000000 INFO @ Tue, 27 Jun 2017 16:37:33: 2000000 INFO @ Tue, 27 Jun 2017 16:37:40: 3000000 INFO @ Tue, 27 Jun 2017 16:37:40: 3000000 INFO @ Tue, 27 Jun 2017 16:37:40: 3000000 INFO @ Tue, 27 Jun 2017 16:37:47: 4000000 INFO @ Tue, 27 Jun 2017 16:37:47: 4000000 INFO @ Tue, 27 Jun 2017 16:37:48: 4000000 INFO @ Tue, 27 Jun 2017 16:37:54: 5000000 INFO @ Tue, 27 Jun 2017 16:37:54: 5000000 INFO @ Tue, 27 Jun 2017 16:37:55: 5000000 INFO @ Tue, 27 Jun 2017 16:38:01: 6000000 INFO @ Tue, 27 Jun 2017 16:38:01: 6000000 INFO @ Tue, 27 Jun 2017 16:38:02: 6000000 INFO @ Tue, 27 Jun 2017 16:38:08: 7000000 INFO @ Tue, 27 Jun 2017 16:38:09: 7000000 INFO @ Tue, 27 Jun 2017 16:38:10: 7000000 INFO @ Tue, 27 Jun 2017 16:38:15: 8000000 INFO @ Tue, 27 Jun 2017 16:38:16: 8000000 INFO @ Tue, 27 Jun 2017 16:38:17: 8000000 INFO @ Tue, 27 Jun 2017 16:38:23: 9000000 INFO @ Tue, 27 Jun 2017 16:38:24: 9000000 INFO @ Tue, 27 Jun 2017 16:38:25: 9000000 INFO @ Tue, 27 Jun 2017 16:38:30: 10000000 INFO @ Tue, 27 Jun 2017 16:38:31: 10000000 INFO @ Tue, 27 Jun 2017 16:38:33: 10000000 INFO @ Tue, 27 Jun 2017 16:38:38: 11000000 INFO @ Tue, 27 Jun 2017 16:38:39: 11000000 INFO @ Tue, 27 Jun 2017 16:38:40: 11000000 INFO @ Tue, 27 Jun 2017 16:38:45: 12000000 INFO @ Tue, 27 Jun 2017 16:38:46: 12000000 INFO @ Tue, 27 Jun 2017 16:38:48: 12000000 INFO @ Tue, 27 Jun 2017 16:38:52: 13000000 INFO @ Tue, 27 Jun 2017 16:38:54: 13000000 INFO @ Tue, 27 Jun 2017 16:38:56: 13000000 INFO @ Tue, 27 Jun 2017 16:39:00: 14000000 INFO @ Tue, 27 Jun 2017 16:39:01: 14000000 INFO @ Tue, 27 Jun 2017 16:39:04: 14000000 INFO @ Tue, 27 Jun 2017 16:39:07: 15000000 INFO @ Tue, 27 Jun 2017 16:39:09: 15000000 INFO @ Tue, 27 Jun 2017 16:39:11: 15000000 INFO @ Tue, 27 Jun 2017 16:39:15: 16000000 INFO @ Tue, 27 Jun 2017 16:39:17: 16000000 INFO @ Tue, 27 Jun 2017 16:39:19: 16000000 INFO @ Tue, 27 Jun 2017 16:39:22: 17000000 INFO @ Tue, 27 Jun 2017 16:39:24: 17000000 INFO @ Tue, 27 Jun 2017 16:39:27: 17000000 INFO @ Tue, 27 Jun 2017 16:39:29: 18000000 INFO @ Tue, 27 Jun 2017 16:39:32: 18000000 INFO @ Tue, 27 Jun 2017 16:39:34: 18000000 INFO @ Tue, 27 Jun 2017 16:39:37: 19000000 INFO @ Tue, 27 Jun 2017 16:39:39: 19000000 INFO @ Tue, 27 Jun 2017 16:39:42: 19000000 INFO @ Tue, 27 Jun 2017 16:39:44: 20000000 INFO @ Tue, 27 Jun 2017 16:39:47: 20000000 INFO @ Tue, 27 Jun 2017 16:39:50: 20000000 INFO @ Tue, 27 Jun 2017 16:39:52: 21000000 INFO @ Tue, 27 Jun 2017 16:39:54: 21000000 INFO @ Tue, 27 Jun 2017 16:39:56: 21000000 INFO @ Tue, 27 Jun 2017 16:39:58: 22000000 INFO @ Tue, 27 Jun 2017 16:40:01: 22000000 INFO @ Tue, 27 Jun 2017 16:40:03: 22000000 INFO @ Tue, 27 Jun 2017 16:40:05: 23000000 INFO @ Tue, 27 Jun 2017 16:40:08: 23000000 INFO @ Tue, 27 Jun 2017 16:40:10: 23000000 INFO @ Tue, 27 Jun 2017 16:40:12: 24000000 INFO @ Tue, 27 Jun 2017 16:40:15: 24000000 INFO @ Tue, 27 Jun 2017 16:40:16: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:40:16: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:40:16: #1 total tags in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:16: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:40:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:40:16: #1 tags after filtering in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:40:16: #1 finished! INFO @ Tue, 27 Jun 2017 16:40:16: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:40:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:40:17: 24000000 INFO @ Tue, 27 Jun 2017 16:40:18: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:40:18: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:40:18: Process for pairing-model is terminated! cat: SRX113317.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113317.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:40:19: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:40:19: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:40:19: #1 total tags in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:19: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:40:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:40:19: #1 tags after filtering in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:40:19: #1 finished! INFO @ Tue, 27 Jun 2017 16:40:19: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:40:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:40:20: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:40:20: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:40:20: #1 total tags in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:20: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:40:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:40:21: #1 tags after filtering in treatment: 24529500 INFO @ Tue, 27 Jun 2017 16:40:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:40:21: #1 finished! INFO @ Tue, 27 Jun 2017 16:40:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:40:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:40:21: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:40:21: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:40:21: Process for pairing-model is terminated! cat: SRX113317.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113317.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:40:22: #2 number of paired peaks: 37 WARNING @ Tue, 27 Jun 2017 16:40:22: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:40:22: Process for pairing-model is terminated! cat: SRX113317.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113317.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113317.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。