Job ID = 1293772 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,833,913 reads read : 31,833,913 reads written : 31,833,913 spots read : 36,935,373 reads read : 36,935,373 reads written : 36,935,373 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:34 68769286 reads; of these: 68769286 (100.00%) were unpaired; of these: 13869589 (20.17%) aligned 0 times 32177706 (46.79%) aligned exactly 1 time 22721991 (33.04%) aligned >1 times 79.83% overall alignment rate Time searching: 00:31:34 Overall time: 00:31:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 20413229 / 54899697 = 0.3718 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 03:06:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:06:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:06:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:06:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:06:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:06:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:06:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 03:06:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 03:06:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 03:07:00: 1000000 INFO @ Mon, 03 Jun 2019 03:07:00: 1000000 INFO @ Mon, 03 Jun 2019 03:07:02: 1000000 INFO @ Mon, 03 Jun 2019 03:07:07: 2000000 INFO @ Mon, 03 Jun 2019 03:07:07: 2000000 INFO @ Mon, 03 Jun 2019 03:07:12: 2000000 INFO @ Mon, 03 Jun 2019 03:07:14: 3000000 INFO @ Mon, 03 Jun 2019 03:07:14: 3000000 INFO @ Mon, 03 Jun 2019 03:07:21: 3000000 INFO @ Mon, 03 Jun 2019 03:07:21: 4000000 INFO @ Mon, 03 Jun 2019 03:07:21: 4000000 INFO @ Mon, 03 Jun 2019 03:07:28: 5000000 INFO @ Mon, 03 Jun 2019 03:07:28: 5000000 INFO @ Mon, 03 Jun 2019 03:07:30: 4000000 INFO @ Mon, 03 Jun 2019 03:07:35: 6000000 INFO @ Mon, 03 Jun 2019 03:07:35: 6000000 INFO @ Mon, 03 Jun 2019 03:07:39: 5000000 INFO @ Mon, 03 Jun 2019 03:07:41: 7000000 INFO @ Mon, 03 Jun 2019 03:07:42: 7000000 INFO @ Mon, 03 Jun 2019 03:07:47: 6000000 INFO @ Mon, 03 Jun 2019 03:07:48: 8000000 INFO @ Mon, 03 Jun 2019 03:07:48: 8000000 INFO @ Mon, 03 Jun 2019 03:07:55: 9000000 INFO @ Mon, 03 Jun 2019 03:07:55: 9000000 INFO @ Mon, 03 Jun 2019 03:07:56: 7000000 INFO @ Mon, 03 Jun 2019 03:08:02: 10000000 INFO @ Mon, 03 Jun 2019 03:08:02: 10000000 INFO @ Mon, 03 Jun 2019 03:08:05: 8000000 INFO @ Mon, 03 Jun 2019 03:08:08: 11000000 INFO @ Mon, 03 Jun 2019 03:08:09: 11000000 INFO @ Mon, 03 Jun 2019 03:08:14: 9000000 INFO @ Mon, 03 Jun 2019 03:08:15: 12000000 INFO @ Mon, 03 Jun 2019 03:08:15: 12000000 INFO @ Mon, 03 Jun 2019 03:08:21: 13000000 INFO @ Mon, 03 Jun 2019 03:08:22: 13000000 INFO @ Mon, 03 Jun 2019 03:08:22: 10000000 INFO @ Mon, 03 Jun 2019 03:08:28: 14000000 INFO @ Mon, 03 Jun 2019 03:08:28: 14000000 INFO @ Mon, 03 Jun 2019 03:08:31: 11000000 INFO @ Mon, 03 Jun 2019 03:08:34: 15000000 INFO @ Mon, 03 Jun 2019 03:08:35: 15000000 INFO @ Mon, 03 Jun 2019 03:08:40: 12000000 INFO @ Mon, 03 Jun 2019 03:08:41: 16000000 INFO @ Mon, 03 Jun 2019 03:08:41: 16000000 INFO @ Mon, 03 Jun 2019 03:08:48: 17000000 INFO @ Mon, 03 Jun 2019 03:08:48: 13000000 INFO @ Mon, 03 Jun 2019 03:08:48: 17000000 INFO @ Mon, 03 Jun 2019 03:08:54: 18000000 INFO @ Mon, 03 Jun 2019 03:08:55: 18000000 INFO @ Mon, 03 Jun 2019 03:08:56: 14000000 INFO @ Mon, 03 Jun 2019 03:09:01: 19000000 INFO @ Mon, 03 Jun 2019 03:09:01: 19000000 INFO @ Mon, 03 Jun 2019 03:09:05: 15000000 INFO @ Mon, 03 Jun 2019 03:09:07: 20000000 INFO @ Mon, 03 Jun 2019 03:09:08: 20000000 INFO @ Mon, 03 Jun 2019 03:09:13: 16000000 INFO @ Mon, 03 Jun 2019 03:09:14: 21000000 INFO @ Mon, 03 Jun 2019 03:09:14: 21000000 INFO @ Mon, 03 Jun 2019 03:09:20: 22000000 INFO @ Mon, 03 Jun 2019 03:09:21: 17000000 INFO @ Mon, 03 Jun 2019 03:09:21: 22000000 INFO @ Mon, 03 Jun 2019 03:09:27: 23000000 INFO @ Mon, 03 Jun 2019 03:09:28: 23000000 INFO @ Mon, 03 Jun 2019 03:09:29: 18000000 INFO @ Mon, 03 Jun 2019 03:09:33: 24000000 INFO @ Mon, 03 Jun 2019 03:09:34: 24000000 INFO @ Mon, 03 Jun 2019 03:09:37: 19000000 INFO @ Mon, 03 Jun 2019 03:09:40: 25000000 INFO @ Mon, 03 Jun 2019 03:09:40: 25000000 INFO @ Mon, 03 Jun 2019 03:09:46: 20000000 INFO @ Mon, 03 Jun 2019 03:09:46: 26000000 INFO @ Mon, 03 Jun 2019 03:09:47: 26000000 INFO @ Mon, 03 Jun 2019 03:09:53: 27000000 INFO @ Mon, 03 Jun 2019 03:09:54: 27000000 INFO @ Mon, 03 Jun 2019 03:09:55: 21000000 INFO @ Mon, 03 Jun 2019 03:09:59: 28000000 INFO @ Mon, 03 Jun 2019 03:10:00: 28000000 INFO @ Mon, 03 Jun 2019 03:10:03: 22000000 INFO @ Mon, 03 Jun 2019 03:10:06: 29000000 INFO @ Mon, 03 Jun 2019 03:10:07: 29000000 INFO @ Mon, 03 Jun 2019 03:10:11: 23000000 INFO @ Mon, 03 Jun 2019 03:10:12: 30000000 INFO @ Mon, 03 Jun 2019 03:10:13: 30000000 INFO @ Mon, 03 Jun 2019 03:10:19: 31000000 INFO @ Mon, 03 Jun 2019 03:10:19: 24000000 INFO @ Mon, 03 Jun 2019 03:10:20: 31000000 INFO @ Mon, 03 Jun 2019 03:10:25: 32000000 INFO @ Mon, 03 Jun 2019 03:10:26: 32000000 INFO @ Mon, 03 Jun 2019 03:10:27: 25000000 INFO @ Mon, 03 Jun 2019 03:10:32: 33000000 INFO @ Mon, 03 Jun 2019 03:10:32: 33000000 INFO @ Mon, 03 Jun 2019 03:10:35: 26000000 INFO @ Mon, 03 Jun 2019 03:10:38: 34000000 INFO @ Mon, 03 Jun 2019 03:10:39: 34000000 INFO @ Mon, 03 Jun 2019 03:10:42: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:10:42: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:10:42: #1 total tags in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:10:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:10:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:10:42: #1 tags after filtering in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:10:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:10:42: #1 finished! INFO @ Mon, 03 Jun 2019 03:10:42: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:10:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:10:43: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:10:43: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:10:43: #1 total tags in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:10:43: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:10:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:10:43: 27000000 INFO @ Mon, 03 Jun 2019 03:10:43: #1 tags after filtering in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:10:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:10:43: #1 finished! INFO @ Mon, 03 Jun 2019 03:10:43: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:10:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:10:45: #2 number of paired peaks: 32 WARNING @ Mon, 03 Jun 2019 03:10:45: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:10:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:10:46: #2 number of paired peaks: 32 WARNING @ Mon, 03 Jun 2019 03:10:46: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:10:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 03:10:50: 28000000 INFO @ Mon, 03 Jun 2019 03:10:59: 29000000 INFO @ Mon, 03 Jun 2019 03:11:08: 30000000 INFO @ Mon, 03 Jun 2019 03:11:16: 31000000 INFO @ Mon, 03 Jun 2019 03:11:24: 32000000 INFO @ Mon, 03 Jun 2019 03:11:32: 33000000 INFO @ Mon, 03 Jun 2019 03:11:40: 34000000 INFO @ Mon, 03 Jun 2019 03:11:44: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 03:11:44: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 03:11:44: #1 total tags in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:11:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 03:11:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 03:11:45: #1 tags after filtering in treatment: 34486468 INFO @ Mon, 03 Jun 2019 03:11:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 03:11:45: #1 finished! INFO @ Mon, 03 Jun 2019 03:11:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 03:11:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 03:11:47: #2 number of paired peaks: 32 WARNING @ Mon, 03 Jun 2019 03:11:47: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 03:11:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113315/SRX113315.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。