Job ID = 1293766 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,747,945 reads read : 27,747,945 reads written : 27,747,945 spots read : 14,700,664 reads read : 14,700,664 reads written : 14,700,664 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:38 42448609 reads; of these: 42448609 (100.00%) were unpaired; of these: 8278223 (19.50%) aligned 0 times 26417115 (62.23%) aligned exactly 1 time 7753271 (18.27%) aligned >1 times 80.50% overall alignment rate Time searching: 00:10:38 Overall time: 00:10:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11426425 / 34170386 = 0.3344 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:41:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:41:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:41:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:41:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:41:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:41:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:41:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:41:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:41:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:41:56: 1000000 INFO @ Mon, 03 Jun 2019 02:41:58: 1000000 INFO @ Mon, 03 Jun 2019 02:41:59: 1000000 INFO @ Mon, 03 Jun 2019 02:42:04: 2000000 INFO @ Mon, 03 Jun 2019 02:42:08: 2000000 INFO @ Mon, 03 Jun 2019 02:42:10: 2000000 INFO @ Mon, 03 Jun 2019 02:42:14: 3000000 INFO @ Mon, 03 Jun 2019 02:42:19: 3000000 INFO @ Mon, 03 Jun 2019 02:42:20: 3000000 INFO @ Mon, 03 Jun 2019 02:42:24: 4000000 INFO @ Mon, 03 Jun 2019 02:42:29: 4000000 INFO @ Mon, 03 Jun 2019 02:42:31: 4000000 INFO @ Mon, 03 Jun 2019 02:42:32: 5000000 INFO @ Mon, 03 Jun 2019 02:42:38: 5000000 INFO @ Mon, 03 Jun 2019 02:42:40: 6000000 INFO @ Mon, 03 Jun 2019 02:42:41: 5000000 INFO @ Mon, 03 Jun 2019 02:42:48: 6000000 INFO @ Mon, 03 Jun 2019 02:42:48: 7000000 INFO @ Mon, 03 Jun 2019 02:42:51: 6000000 INFO @ Mon, 03 Jun 2019 02:42:56: 8000000 INFO @ Mon, 03 Jun 2019 02:42:57: 7000000 INFO @ Mon, 03 Jun 2019 02:43:00: 7000000 INFO @ Mon, 03 Jun 2019 02:43:06: 9000000 INFO @ Mon, 03 Jun 2019 02:43:06: 8000000 INFO @ Mon, 03 Jun 2019 02:43:10: 8000000 INFO @ Mon, 03 Jun 2019 02:43:13: 10000000 INFO @ Mon, 03 Jun 2019 02:43:16: 9000000 INFO @ Mon, 03 Jun 2019 02:43:20: 9000000 INFO @ Mon, 03 Jun 2019 02:43:20: 11000000 INFO @ Mon, 03 Jun 2019 02:43:25: 10000000 INFO @ Mon, 03 Jun 2019 02:43:29: 12000000 INFO @ Mon, 03 Jun 2019 02:43:30: 10000000 INFO @ Mon, 03 Jun 2019 02:43:34: 11000000 INFO @ Mon, 03 Jun 2019 02:43:40: 11000000 INFO @ Mon, 03 Jun 2019 02:43:40: 13000000 INFO @ Mon, 03 Jun 2019 02:43:44: 12000000 INFO @ Mon, 03 Jun 2019 02:43:48: 14000000 INFO @ Mon, 03 Jun 2019 02:43:49: 12000000 INFO @ Mon, 03 Jun 2019 02:43:53: 13000000 INFO @ Mon, 03 Jun 2019 02:43:56: 15000000 INFO @ Mon, 03 Jun 2019 02:43:59: 13000000 INFO @ Mon, 03 Jun 2019 02:44:02: 14000000 INFO @ Mon, 03 Jun 2019 02:44:04: 16000000 INFO @ Mon, 03 Jun 2019 02:44:08: 14000000 INFO @ Mon, 03 Jun 2019 02:44:11: 15000000 INFO @ Mon, 03 Jun 2019 02:44:12: 17000000 INFO @ Mon, 03 Jun 2019 02:44:17: 15000000 INFO @ Mon, 03 Jun 2019 02:44:21: 16000000 INFO @ Mon, 03 Jun 2019 02:44:22: 18000000 INFO @ Mon, 03 Jun 2019 02:44:27: 16000000 INFO @ Mon, 03 Jun 2019 02:44:30: 19000000 INFO @ Mon, 03 Jun 2019 02:44:30: 17000000 INFO @ Mon, 03 Jun 2019 02:44:38: 20000000 INFO @ Mon, 03 Jun 2019 02:44:38: 17000000 INFO @ Mon, 03 Jun 2019 02:44:39: 18000000 INFO @ Mon, 03 Jun 2019 02:44:45: 21000000 INFO @ Mon, 03 Jun 2019 02:44:47: 18000000 INFO @ Mon, 03 Jun 2019 02:44:49: 19000000 INFO @ Mon, 03 Jun 2019 02:44:53: 22000000 INFO @ Mon, 03 Jun 2019 02:44:58: 19000000 INFO @ Mon, 03 Jun 2019 02:44:58: 20000000 INFO @ Mon, 03 Jun 2019 02:45:00: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:45:00: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:45:00: #1 total tags in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:45:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:45:00: #1 tags after filtering in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:45:00: #1 finished! INFO @ Mon, 03 Jun 2019 02:45:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:45:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:45:02: #2 number of paired peaks: 44 WARNING @ Mon, 03 Jun 2019 02:45:02: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:45:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:45:07: 20000000 INFO @ Mon, 03 Jun 2019 02:45:07: 21000000 INFO @ Mon, 03 Jun 2019 02:45:16: 22000000 INFO @ Mon, 03 Jun 2019 02:45:16: 21000000 INFO @ Mon, 03 Jun 2019 02:45:23: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:45:23: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:45:23: #1 total tags in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:45:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:45:23: #1 tags after filtering in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:45:23: #1 finished! INFO @ Mon, 03 Jun 2019 02:45:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:45:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:45:25: #2 number of paired peaks: 44 WARNING @ Mon, 03 Jun 2019 02:45:25: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:45:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:45:26: 22000000 INFO @ Mon, 03 Jun 2019 02:45:32: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:45:32: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:45:32: #1 total tags in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:45:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:45:33: #1 tags after filtering in treatment: 22743961 INFO @ Mon, 03 Jun 2019 02:45:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:45:33: #1 finished! INFO @ Mon, 03 Jun 2019 02:45:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:45:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:45:35: #2 number of paired peaks: 44 WARNING @ Mon, 03 Jun 2019 02:45:35: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:45:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX113307/SRX113307.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。